Antiangiogenic activity of low-temperature lysozyme from a marine bacterium <Emphasis Type="Italic">in vivo</Emphasis> and <Emphasis Type="Italic">in vitro</Emphasis>

We extracted marine low-temperature lysozyme (MLTL), a novel lysozyme, from a marine microorganism through fermentation. Our previous study suggested that a low molecular weight (16 kDa) may exert anti-tumor activity through antiangiogenesis. In this study, we extracted a high weight (39 kDa) and investigated its antiangiogenic activity in vivo and in vitro. Using zebrafish embryos as an in vivo study model, we found that treatment with MLTL significantly inhibited the growth of subintestinal vessels (SIVs) in a dose-dependent manner and that 400 μg/ml MLTL was sufficient to block the growth of SIVs. An in vitro study conducted using human umbilical vein endothelial cells (HUVECs) revealed that MLTL suppressed the proliferation, migration and tube formation of HUVECs in a dose-dependent manner. Interestingly, assays by flow cytometry and DNA electrophoresis indicated that MLTL was able to induce apoptosis of HUVECs. Moreover, further study demonstrated that the disruption of intracellular Ca²⁺ homeostasis may play an important role in MLTL induced apoptosis of HUVECs. Taken together, the results of this study demonstrate for the first time that MLTL inhibits angiogenesis through its pleiotropic effects on vascular endothelial cells and induces apoptosis through regulation of cellular Ca²⁺ levels. The results of this study also revealed a possible mechanism underlying the antiangiogenic effect of MLTL and suggested that MLTL may be a promising new antiangiogenic agent for use in cancer therapy.     

Distribution and characteristics of volcanic reservoirs in China

About forty productive oil/gas fields hosted in volcanic reservoirs have been found since 1957 in fourteen basins of China. They can be simply subdivided into two groups, the east and the west. Reservoir volcanic rocks of the east group are predominantly composed of Late Jurassic to Early Cretaceous rhyolite and Tertiary basalt, preferred being considered as rift type volcanics developed in the circum-Pacific tectonic regime. Those of the west are Permo-Carboniferous intermediate/basic volcanic rocks, being island-arc type ones developed in paleo-Asian Ocean tectonic regime.     

Predicting size effect on diffusion-limited current density of oxygen reduction by copper wire

The size effect of copper wire radius (0.04–0.82 mm) on the diffusion-limited current density of an oxygen reduction reaction in stagnant simulated seawater (naturally aerated 0.5 mol/L NaCl) is investigated by potentiodynamic polarization and electrochemical impedance spectroscopy (EIS) and compared with the results obtained in 0.5 mol/L H2SO4. In the oxygen diffusion-limited range, size effect is found to occur independent of electrolytes, which is attributed to non-linear diffusion. Additionally, to sati...     

松辽盆地太东斜坡区葡萄花油层油水分布规律及主控因素

太东斜坡区为松辽盆地大庆长垣向三肇凹陷倾没的单倾斜坡.利用岩心、测井和地震等资料,研究葡萄花油层油藏类型和油水分布规律.结果表明:斜坡区油平面受4条近南北向"断层带"分割,具有东西分带特征,且受源外油源供给不足和油东西向运移影响,自东向西单一"断阶带"含油性逐级变差;油垂向分布受沉积演化和优势输导通道控制表现为"双峰"特征,主要分布在葡萄花油层中部;油藏类型主要为东南倾斜坡背景下的近南北向断层与北西向河道砂体匹配形成的断层—岩性油藏.在油水分布规律研究的基础上,结合单一圈闭内断层与单砂体匹配样式,提出源外斜坡区油聚集机制受构造部位、优质储层、断层封闭性和断层与砂体匹配样式控制,即单一"断阶带"高点控制油气聚集部位;浅水三角洲水下分流河道和河控席状砂构成油气优质储层;断层侧向封闭性影响油气垂向富集高度和平面"互补"特征;断层与单砂体匹配样式决定油气富集层位.     

Occurrence and Diversity of Candida Genus in Marine Environments

A total of 317 yeast isolates from seawater,sediments,mud of salterns,guts of marine fishes and marine algae wereobtained.The results of routine identification and molecular characterization showed that six isolates among these marine yeastsbelonged to Candida genus as Candida interrnedia for YA01a,Candida parapsilosis for 3eA2,Candida quercitrusa for JHSb,Can-die rugosa for wl8,Candida zeylanoides for TJY13a,and Candida membranifaciens for W14-3.Isolates YA01a (Candida interme-die),wl8 (Candida rugosa),3eA2 (Candida parapsilosis),and JHSb (Candida quercitrusa) were found producing cell-bound lipase,while isolate W14-3 (Candida membranifaciens) producing riboflavin.These marine yeast Candida spp.Seem to have wide potentialapplications in biotechnology.     

鄂尔多斯盆地苏里格南部地区盒8段沉积相特征及其意义

依据野外露头、岩芯、测井及相关测试资料,对鄂尔多斯盆地苏里格南部地区上古生界中二叠统石盒子组盒8沉积期的沉积类型、沉积微相特征及沉积相对天然气成藏富集的影响进行了分析。结果表明:鄂尔多斯盆地苏里格南部地区盒8沉积期自北向南依次发育缓坡浅水辫状河三角洲和滨浅湖沉积相,亚相主要为三角洲平原、三角洲前缘、滨湖和浅湖,微相包括分流河道、分流间洼地、水下分流河道、水下天然堤、分流间湾、滨湖砂坝、滨湖泥湾和浅湖泥湾;各微相在空间上相互叠置,复合加积;各微相水动力条件的差异造成微相在沉积物成分和组构上各异,导致其成岩作用不同;分流河道和水下分流河道微相发育处密集形成相互叠置的大面积骨架砂体,构成区内主砂带,成为天然气优质储集层,是有利勘探相带;沉积相明显控制优质储集层的分布,进而影响着天然气的富集成藏。总之,水下分流河道和分流河道微相是砂岩优质储集层分布和发育的最有利相带,也是今后勘探开发的方向。     

Field experiments of multi-channel oceanographic fluorescence lidar for oil spill and chlorophyll-a detection

A Multi-channel Oceanographic Fluorescence Lidar (MOFL), with a UV excitation at 355 nm and multiple receiving channels at typical wavelengths of fluorescence from oil spills and chlorophyll-a (Chl-a), has been developed using the Laser-induced Fluorescence (LIF) technique. The sketch of the MOFL system equipped with a compact multi-channel photomultiplier tube (MPMT) is introduced in the paper. The methods of differentiating the oil fluorescence from the background water fluorescence and evaluating the Chl-a concentration are described. Two field experiments were carried out to investigate the field performance of the system, i.e., an experiment in coastal areas for oil pollution detection and an experiment over the Yellow Sea for Chl-a monitoring. In the coastal experiment, several oil samples and other fluorescence substances were used to analyze the fluorescence spectral characteristics for oil identification, and to estimate the thickness of oil films at the water surface. The experiment shows that both the spectral shape of fluorescence induced from surface water and the intensity ratio of two channels (I ₄₉₅/I ₄₀₅) are essential to determine oil-spill occurrence. In the airborne experiment, MOFL was applied to measure relative Chl-a concentrations in the upper layer of the ocean. A comparison of relative Chl-a concentration measurements by MOFL and the Moderate Resolution Imaging Spectroradiometer (MODIS) indicates that the two datasets are in good agreement. The results show that the MOFL system is capable of monitoring oil spills and Chl-a in the upper layer of ocean water.     

水资源开发利用引起的环境负效应——以玛纳斯河流域为例

通过对玛纳斯河流域环境负效应研究,确定了水资源开发利用过程中引起环境负效应的制约因子,引入地下水动态均衡方法进行半定量分析,明确地下水量的变化为引起环境负效应的直接因素,为定量进行水资源综合开发与利用、防止环境负效应的产生提供方法和依据。     

桑塔木地区三叠系曲流河三角洲存在证据与沉积特征

为了厘清桑塔木地区三叠系砂体成因类型,指导油气勘探,利用岩心、钻测井、分析化验及高精度三维地震资料,研究碎屑岩的粒度、结构、沉积构造、砂体的空间展布、地震反射特征等.结果表明:三叠系TⅡ油组具有粒度细、分选磨圆好、发育小型交错层理、波状层理及水平层理,呈指状产出,地震剖面上具有前积反射构型,典型相干体地层切片见曲流特征的特点,是典型的曲流河三角洲沉积;同时,研究区曲流河三角洲发育2种亚相6种微相,其中平原亚相发育天然堤、水上分流河道及河漫沼泽微相,前缘亚相发育水下分流河道、河口坝和支流间湾3种微相;区内TⅡ油组以发育北部和西南部2个主要方向的曲流河三角洲为主,其中北部三角洲规模大,平原和前缘亚相较发育,西南部三角洲以前缘亚相为主,平原亚相以分流河道沉积为主,前缘亚相主要发育分流河道和河口坝微相.该三角洲的形成与地形坡度及气候的变化密切相关.     

烟台芝罘湾底质沉积物粒度特征和沉积动力环境研究

根据芝罘湾海域110个底质沉积物样品粒度分析结果,对沉积物类型和粒度参数分布进行了总结。在此基础上,利用粒径趋势分析模型(GSTA)和沉积动力判别图解,探讨了底质沉积物的运移趋势及沉积水动力规律。研究表明,芝罘湾海域底质沉积物类型共有8种,其中砂质泥、砂质粉砂、泥和粉砂分布最广泛;平均粒径值具有从岸向海逐渐增大的趋势,分选性、偏度和峰度分别为差、正偏—极正偏、平坦—中等的特征;粒度运移趋势分析反映出套子湾泥沙向西、西南运移,芝罘湾及以东海域泥沙呈顺时针方向运移;沉积动力判别图解揭示出芝罘湾海域沉积水动力具有整体较弱的特征。