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91.
92.
Amphioxus, a cephalochordate, is an important model fish for studies in evolution and comparative biology. A successful cell
culture from amphioxus tissues in vitro would help understanding some basic issues. To determine the optimal culture conditions for proliferation of amphioxus cells,
primary cultures were initiated from buccal cirri, tail, gill, gut and metapleural fold of amphioxus Branchiostoma belcheri tsingtauense. The media tested were L-15, F-12, M 199, MEM, DMEM, PRMI 1640 and LDF, each was supplemented with 20% fetal bovine serum.
The optimal conditions include tail tissue cultured in L-15 or F-12 with supplement of 20% FBS and 1.5% NaCl at about 25°C.
Supported by Doctoral Initial Fund of Ludong University (No.43304) 相似文献
93.
The way in which rocks and engineering materials heat‐up and dry‐out in the intertidal zone is of relevance to both weathering and ecology. These behaviours can be measured in the laboratory under controlled conditions designed to replicate those occurring in the field. Previous studies have demonstrated differences in thermal behaviours between rock types and through time as a result of soiling in terrestrial environments, but the influence of weathering and colonization on rock behaviours in the intertidal zone has not been previously assessed. We measured the warming and drying of blocks of rock (limestone and granite) and marine concrete during ‘low‐tide’ events simulated in the laboratory, before and after a period of exposure (eight months) on rock platforms in Cornwall, UK. As well as differences between the material types, temperatures of control (unexposed) and field‐exposed blocks differed in the order of 1 to 2 °C. Drying behaviours were also different after field exposure. Differences during the first few hours of exposure to air and heat were attributed to discolouration and albedo effects. Over longer periods of time, changes in the availability of near‐surface pore water as a result of micro‐scale bioerosion of limestone and the development of bio‐chemical crusts on marine concrete [observed using scanning electron microscopy (SEM)] are suggested as mechanisms enhancing and reducing, respectively, the efficiency of evaporative cooling. The retention of moisture by epilithic biofilms may also influence thermal and drying behaviours of granite. These observations represent one of the first examples of cross‐scalar biogeomorphic linkages in the intertidal zone. The significance of the results for the subsequent efficiency of weathering, and near‐surface micro‐climatic conditions experienced by colonizing organisms is discussed. The involvement of microorganisms in the creation of more (or less) ecologically stressful conditions through the alteration of substratum geomorphic properties and behaviours is suggested as an example of ‘biogeomorphic ecosystem engineering’. Copyright © 2011 John Wiley & Sons, Ltd. 相似文献
94.
95.
新近纪以来的构造运动是中国气藏形成的重要因素 总被引:14,自引:2,他引:14
新近纪以来的构造运动是全球最新的地壳形变和造山运动的发展演化阶段。由于中国地处印度陆块、西伯利亚陆块与太平洋板块所围限的三角地带。是世界上新近纪以来构造运动比较活跃的地区,以及天然气极易散失,也难以保存的特点,因此,新近纪以来构造运动特征是研究中国气藏形成和保存不可忽视的重要地质因素。总结中国天然气的成藏历程,虽然各类盆地有所不同,但都有上新世以来构造运动的影响。上新世以来的构造运动对于中国天然气的成藏及分布有一定的破坏作用,促使一些盆地天然气受到了一定程度的散失,但总体上以促进成藏、聚集作用为主:①在西部盆岭之间形成了一系列中国式的前陆型盆地,为形成大中型气田刨造了有利条件。并控制了盆地本部气藏的最终定型。②在中部的四川盆地形成了成排成带的背斜,是四川盆地气藏形成、定型的主要时期;鄂尔多斯盆地的整体快速隆升及进一步西倾,促进了从靖边至乌审旗气田群天然气的进一步聚集。③在东部,上新世末期的渤海运动为以渤中拗陷为主体的渤海海域晚期成藏刨造了有利的地质条件;在其他盆地也促进了二次生烃及次生气藏的形成;并形成了一批无机成因的二氧化碳气田。④在近海海域,既促进了有机成因气藏的形成,也促进了无机成因非烃气藏以及无机与有机成因天然气的混源,致使区内形成了比较复杂的气藏特征。总之,新近纪以来活跃的构造运动形成了中国天然气以晚期、超晚期生烃成藏、定型为主的成藏模式,是研究中国气藏形成不可忽视的重要的地质阶段。 相似文献
96.
黄河中游岩土侵蚀问题及防治对策探讨 总被引:1,自引:0,他引:1
黄河中游出露地层岩性主要有古生界奥陶系碳酸盐岩、石炭系和二叠系碎屑岩;中生界三叠系砂泥岩、侏罗系砂泥岩和白垩系砂泥岩;新生界古近系砂泥岩、新近系(原为第三系)红土及第四系风积黄土和现代风积沙。第四系黄土厚度大,结构松散,遇水易解体,具湿陷性,是黄河中游粗泥砂的主要物源;第四系风积沙分布于黄河中游北部,亦是黄河中游粗泥砂的主要物源。岩土侵蚀类型有五:一是风力侵蚀—风沙侵蚀;二是降雨对谷坡面的面状冲刷—坡面侵蚀;三是地表径流对谷底的侧蚀—沟谷侵蚀;四是滑塌对谷坡坡体的破坏—重力侵蚀;五是潜蚀。并对不同侵蚀类型提出了不同的防治对策。 相似文献
97.
98.
滑坡灾害成因机理复杂、影响因素众多,深度学习作为当前人工智能领域的热点,能够更好地模拟滑坡灾害的形成并准确预测潜在的斜坡。为了挖掘深度学习在滑坡易发性的应用潜能,本文构建了一维、二维和三维的滑坡数据表达形式,并提出3种基于卷积神经网络模型(Convolutional Neural Networks, CNN)的滑坡易发性分析处理框架:基于CNN分类器、基于CNN与逻辑回归的融合和基于CNN集成,最后以江西省铅山县为研究对象进行验证,结果表明:所有基于CNN的易发性模型都能够获得准确且可靠的滑坡易发性分析结果。其中,基于二维数据的CNN模型在所有单分类器中预测精度最高,为78.95%。此外,二维CNN特征提取能够显著提升逻辑回归的预测精度,其准确率提升7.9%。最后,异质集成策略能够大幅度提升基于CNN分类器的滑坡预测精度,其准确率提升4.35%~8.78%。 相似文献
99.
In this study, 16 combinations of the ECMWF (European Centre for Medium-Range Weather Forecast) reanalyzed daily rainfall and the pentad CMAP in China for the period 1980- 1993( 1May - 31 Dec. ) were calculated. Correlation analysis was used to roughly evaluate daily rainfall for the whole of China and a combination of RPC (rotated principal component) and wavelet analyses was applied to data on observed and combined daily rainfall to obtain a detailed evaluation of the quality of these combined datasets in 6 selected major rainfall regions of eastern China. The results showed that except for intraweekly fluctuation, the best combination was roughly similar to or accorded well with observation in the aspects of space variation patterns and long period rainfall fluctuations related to monsoon onset and serious meteorologic disasters, indicating that this combination yielded better values of long term daily mean and standard deviation through the pentad CMAP ( CPC Merged Analysis of Precipitation), and can also represent rainfall fluctuations through the reanalyzed daily rainfall. 相似文献
100.
研究了麻城地区重、磁异常场特征,经反演计算获得了深部结构特征。对重力布格异常利用Parker-Oldenburg快速位场反演方法计算出莫霍面的展布,对航磁异常利用对数功率谱法和矩谱法计算出居里面深度。在此基础上,探讨该震区深部蕴震条件。主要结论是:①麻城-团凤断裂带为该区切割较深的主要控震构造;②1932年麻城6.0级地震发生在区域重力异常等值线梯度最大处,磁力异常区两局部正异常间的低值带,莫霍面及居里面隆起区边缘;③发震部位有其特定的深部蕴震构造及应力蕴震条件。 相似文献