产杀虫活性物质海洋放线菌的筛选和初步鉴定

从浙江宁波海域的滩涂泥样中分离到54株海洋放线菌,用卤虫液体测定法进行了杀虫活性菌株的筛选,结果显示多株放线菌具有较高的杀虫活性,菌株XS902,XS904,BL803,BL807对卤虫的ID50高达100以上。同时研究了XS904活性菌株的形态培养特征和生理生化特征,初步鉴定该菌株属于链霉菌属。     

Risk identification and perception in the fisheries sector: Comparisons between the Faroes,Greece, Iceland and UK

This paper presents the findings of risk identification and risk perception research conducted in relation to the fisheries systems of four distinct and diverse European countries: Faroes, Iceland, Greece and the UK. Risk research traditionally attempts to quantify the potential threat or consequences from a range of risk events or hazards. This research, however, adopted a social sciences perspective and so assumed that a risk event or hazard can mean different things to different people and that these perceptions are also context and culturally dependent. Risk perceptions were examined and risk registers developed in each country for a range of stakeholder groups. A ‘mental modelling’ approach was adopted in a series of qualitative interviews. Findings were examined in terms of a wide range of psychological, social and cultural risk theories. Differences in risk perceptions were noted between stakeholder groups and countries and contextual influences were examined such as the widely differing fisheries management systems used in each country. This research provides one of the first attempts to systematically evaluate risks and perceptions across a range of fisheries-systems. The findings support social science theories which argue that risk is a subjective, as opposed to objective, concept and that this subjectivity will therefore affect our attempts to assess and manage those hazards we think we can potentially influence or control.     

地震平点技术与应用进展

利用平点技术进行地震勘探虽然经历了50多年的发展历程,但是国内外尚缺乏对平点技术与应用进展的系统分析。通过对平点技术的基本原理、平点识别方法、平点技术应用进展及应用"陷阱"进行全面的调研与梳理认为,不同流体纵波阻抗的差异是产生平点反射的基本条件;水平反射与AVO振幅异常突变处吻合并且具有统一的深度值,是烃类平点最重要的判别标准;通过地震反射特征,结合共等值线抽道集叠加技术和基于信号强化处理的平点增强技术等,可以提高平点的识别效果;地震资料产生的假象、非储层因素导致的平点假象以及流体界面由于速度和压力的变化都会导致平点"陷阱"的产生。研究指出,加强地质与地球物理综合分析,进一步提高地震资料的保真度、信噪比和分辨率,加强与其他烃类检测方法的综合应用及大力发展多波多分量地震技术在平点勘探中的应用是平点技术的发展趋势,未来其应用必然会更加广泛。     

一例异育银鲫(Carassius auratus gibelio)暴发性出血病病原分析

2016年6月,江苏某异育银鲫(Carassius auratus gibelio)养殖场暴发一种传染性急性出血病,造成养殖银鲫大量死亡。为分析此次疾病病因及流行规律,本研究从发病养殖场采集患出血病的异育银鲫,从细菌、病毒及寄生虫三个方面对病原进行了分析。采用病原菌分离、组织病理学观察、超薄切片电镜观察、病毒核酸分析、回感实验等对病原进行鉴定。结果显示从发病鲫鱼体内分离到病毒一株,未发现寄生虫及细菌感染。经测序及序列分析,该病毒为鲤疱疹病毒Ⅱ型(Cyprinid herpesvirus2,CyHV-2)病毒,组织病理学观察结果显示患病鱼的鳃和肾脏有明显病变,电镜下可观察到病鱼脾脏组织有带囊膜的球形病毒,囊膜直径约为170—200nm,病毒衣壳直径约为110—120nm,核心直径约为60nm,用组织匀浆感染鲫鱼囊胚细胞系(CGB)可稳定地观察到典型的细胞病变,用患病鱼组织匀浆液人工感染异育银鲫的死亡率高达100%,荧光定量PCR检测到该病毒可感染多器官,其中以脾脏中病毒含量最高,其次是脑,肝脏中最少。本研究可为CyHV-2的诊断防控及疫苗研制提供资料。     

Establishment and application of an intelligent treating method for oil spill identification

In the identifying process of an oil spill accident, manual integral and artificial visual comparison are commonly used at present to determine the oil spill sources, these methods are time-consuming and easily affected by human factors. Therefore, it is difficult to achieve the purpose of rapid identification of an oil spill accident. In this paper, an intelligent method of automatic recognition, integration and calculation of diagnostic ratio of Gas Chromatography-Mass Spectrometer (GC/MS) spectrum are established. Firstly, four hundreds of samples collected around the world were analyzed using a standard method and Retention time locking technology (RTL) was applied to reduce the change of retention time of GC/MS spectrum. Secondly, the automatic identification, integration of n-alkanes, biomarker compounds, polycyclic aromatic hydrocarbons and calculation of the diagnostic ratios were realized by MATLAB software. Finally, a database of oil fingerprints were established and applied successfully in a spill oil accident. Based on the new method and database, we could acquire the diagnostic ratios of an oil sample and find out the suspected oil within a few minutes. This method and database can improve the efficiency in spilled oil identification.     

基于GF-3的不同极化方式SAR图像海面油膜识别的比较 ——以"桑吉"轮事故为例

基于我国自主研发的首颗分辨率达到1m的C频段多极化合成孔径雷达高分三号卫星(GF-3),通过典型溢油案例定性和定量地比较了双极化与单极化图像预处理性能,分析了不同的极化组合对海面油膜识别结果的影响。得出结论:同向极化与异向极化成像差异明显,VV极化明显比VH极化回波强度高,并且油膜识别度较高;相反,双极化VHVV方式与单极化VH方式图像效果区别不大,双极化方式并不能绝对性地提高地物识别度。     

台湾以东黑潮路径识别与变化规律

为研究对中国台湾以东海域黑潮路径及其变化,本文基于法国空间局AVISO中心提供的1993—2015年的卫星遥感海表流场逐日资料,对121°—125°E,22.4°—25°N海域黑潮路径进行了逐日识别,得到了共计23年累计8400天的台湾以东黑潮流轴的逐日路径,并研究其在不同纬度的流轴位置及其对应的表面黑潮流量的时空变化规律。主要结论如下:(1)采用模糊C-均值聚类法对台湾以东黑潮流轴路径进行聚类分析,发现台湾以东黑潮流轴在24°N以南出现明显摆动,形成正常和偏东两种路径;黑潮流轴存在明显的时间变化,流轴偏东现象年平均大约出现25次,大致每隔3年出现一次偏东较少的现象,各月流轴偏东次数以4、5月最少,10月至次年3月较多;(2)台湾以东黑潮表面流量大小在6.2—8.3×104m2/s之间;总体上来说,纬度越高流量越大,在23.5°N左右范围内存在一个流量低值中心;在24.3°N以北流量总体较大,且增长趋势稳定,同时表面流量大小具有较强的季节和年际变化特征。     

南海北部珠江口盆地深水区碳酸盐岩发育特征及地震识别

由于南海的多次扩张、拉伸、消减运动,在珠江口盆地白云深水区发生了陆架裂陷和海水入侵等地质现象,形成了海相碳酸盐岩生长发育的基础地质条件。对珠江口盆地深水区碳酸盐岩的发育地质背景、地质特征、发育规律、地震响应特征以及深水区灰岩发育的主控因素进行了探讨,结果表明白云深水区主要发育两种类型的碳酸盐台地;古隆起镶边台地以及火山建隆孤立台地。台地沉积主要发育在古隆起周缘、构造高部位、火山隆起周缘,并被钻井所证实。在白云深水区东沙隆起西南缘、云荔低凸起、荔湾凹陷北缘、荔湾凹陷南缘及顺鹤隆起发育典型的生物礁,钻井证实有珊瑚、红藻、苔藓虫等造礁生物。生物礁地震特征具有强的连续反射顶、底界面,丘状反射外形,内部结构或空白、或杂乱,具有前积、加积、退积等层序结构。     

柳珊瑚Leptogorgia rigida中甾体类成分研究

首次对南海柳珊瑚Leptogorgia rigida中甾体类次生代谢产物进行研究。采用硅胶柱柱层析、十八烷基硅烷键合硅胶填料、Sephadex LH-20、高效液相色谱(HPLC)及薄层色谱(TLC)等多种方法进行分离纯化, 通过核磁共振波谱(NMR)、质谱(MS)等现代波谱分析及物理常数对照等方法进行结构鉴定。从南海柳珊瑚Leptogorgia rigida次生代谢产物中分离得到8个甾类化合物, 结构依次为: 3β, 6β, 11-trihydroxy-9, 11-seco-5α-chloest-7-en-9-one (1); 3β, 6α, 11- trihydroxy-9, 11-seco-5α-chloest-7-en-9-one (2); 3β, 6α, 11-trihydroxy-5α-chloest-7, 22(E)-dien-9-one (3); pregnan-4-en-3, 20-dione (4); 5α-pregnan-3, 20-dione (5); 3β-hydroxy-pregnan-5-en-20-dion (6); 3β-hydroxy-5α-pregnan-20-one (7); 和3β, 6α, 11-trihydroxy-9, 11-seco-5α-gorgosterol (8)。所有化合物均首次从Leptogorgia属中得到。