Expression of putative zinc-finger protein lcn61 gene in lymphocystis disease virus China （LCDV-cn） genome

An open reading frame (lcn61) of lymphocystis disease virus China (LCDV-cn), probably responsible for encoding putative zinc-finger proteins was amplified and inserted into pET24a (+) vector. Then it expressed in E. coli BL21 (DE3), and His-tag fusion protein of high yield was obtained. It was found that the fusion protein existed in E. coli mainly as inclusion bodies. The bioinformatics analysis indicates that LCN61 is C2H2 type zinc-finger protein containing four C2H2 zinc-finger motifs. This work provides a theory for functional research of lcn61 gene. Supported by High Technology Research and Development Program of China (863 Program, No. 2006AA100309)     

微分算子展开与耦合势三维有限体积法快速计算随钻超深前视电阻率测井响应非线性Born逼近

本文将非均质各向异性地层中电磁场耦合势Helmhotz方程分解为各向异性背景场方程与散射场方程,并应用算子展开技术推导出耦合势Born级数解,建立一套三维非均质各向异性地层中随钻超深前视电阻率测井响应各阶散射电磁场与非线性Born逼近的有效计算方法.首先,利用传输线法与二维插值技术确定背景电磁场空间分布,得到零阶Born逼近解,然后根据电磁场耦合势的Born级数解推导出各阶散射电磁场方程与递推关系,通过三维有限体积法实现各阶散射场耦合势方程的离散,在此基础上结合MKL PARDISO并行技术,通过递推方式逐步计算各阶散射电磁场,并根据Born级数部分和确定不同阶次的非线性Born逼近.最后利用数值模拟结果对正演算法加以检验,并深入研究分析各阶散射场的变化特征与Born级数的收敛性质.

     

Laboratory study of deep-water breaking waves

In an attempt to elucidate the mechanics of deep-water wave breaking, a variety of breaking waves, including spilling and plunging waves, of different length scales and geometries was studied. The waves were generated through wave-wave interactions using wave packets with constant-steepness components, constant-amplitude components, and also components following the Pierson-Moskowitz distribution. Wave steepening prior to breaking were found to cause an increase in the high frequency spectral slope of the wave spectrum. The slopes were correlated to the type of breaking and the intensity of the breaking. The energy loss through breaking varied with the spectral characteristics of the wave packet. On the other hand, it was also noted that, irrespective of the wave packet, the losses were from the higher frequency end of the first harmonics.     

Estimated mass and stiffness matrices of shear building from modal test data

A method that estimates mass and stiffness matrices of shear building from modal test data is presented in this paper. The method depends on only measurable points that are less in number than the total structural degrees of freedom, and on the first two orders of structural mode measured. So it is applicable to most of the general test. Based on this method modal data of unmeasurable points are estimated, then global mass and stiffness matrices of structure are obtained by using the first two orders of modal data. Taking advantage of iteration the optimum global mass and stiffness matrices are gained. Finally, an example is studied in this paper. Its result shows that this method is reliable. © 1998 John Wiley & Sons, Ltd.     

Finite element analysis of the effect of corrosion on the behavior of reinforced earth walls

A simple model for the corrosion‐induced loss of stiffness and strength of the steel strips of earth‐reinforced walls was introduced in a finite element simulation of the long‐term behavior of the wall, in which the backfill‐strips interactions are taken into account by means of a generalized homogenization procedure (called a multiphase model). The results show an initial phase of slow displacements induced by the loss of stiffness, followed after a few decades by a steep acceleration of the displacements, leading to wall failure. The influences of the parameter controlling corrosion, the backfill cohesion and the heterogeneity of the corrosion process are discussed. Results are used to discuss a strategy for reinforced earth wall surveillance. Copyright © 2011 John Wiley & Sons, Ltd.     

Application of saddlepoint approximation in reliability analysis of dynamic systems

The application of the saddlepoint approximation to reliability analysis of dynamic systems is investigated. The failure event in reliability problems is formulated as the exceedance of a single performance variable over a prescribed threshold level. The saddlepoint approximation technique provides a choice to estimate the cumulative distribution function (CDF) of the performance variable. The failure probability is obtained as the value of the complement CDF at a specif ied threshold. The method requires computing the saddlepoint from a simple algebraic equation that depends on the cumulant generating function (CGF) of the performance variable. A method for calculating the saddlepoint using random samples of the performance variable is presented. The applicable region of the saddlepoint approximation is discussed in detail. A 10-story shear building model with white noise excitation illustrates the accuracy and effi ciency of the proposed methodology.     

Point-block covariance: A general solution for the two-dimensional stationary random functions

The application of the theory of random functions to problems of ore evaluation may involve computations of the covariance between the mean value of a given block and the functional value at a given point. However, an analytical solution for such a covariance does not exist for nonspherical blocks and for commonly applied models of covariance functions. Further, because this covariance is a function of the spatial arrangements of the block and the point, it has to be evaluated numerically each time for given point—block arrangements. This paper presents a readily available general solution to this problem in the form of a series of graduated curves which, together with some geometric manipulations, may be used to compute the covariance between a pointand a two-dimensional block for all possible point—block arrangements. The availability of the graph thus eliminates the necessity of using the time-absorbing programs on computers for such computations. Finally, many of the approximations that are made in order to avoid cumbersome covariance evaluations are no longer necessary due to the ease of such computations with the help of the graph provided.     

基于Matlab的断裂带温泉水地球化学特征及地震活动性研究

基于Matlab开发出地下流体分析软件,对比分析了甘肃西秦岭北缘的武山、街子和清水温泉水化学组分特征;初步分析了温泉水水质类型、补给来源、水-岩平衡状态以及循环深度,并讨论了温泉水深循环对地震活动性的影响.研究表明温泉水均为大气降水成因,其水化特征受围岩的控制作用;清水温泉水的成熟度没有武山和街子温泉水高.地下水循环深度较浅的武山温泉和街子温泉水化类型属于Na-HCO3·SO4·Cl,所处断裂的地震活动频繁;地下水循环深度较深的清水温泉水化类型属于Na-SO4·Cl,所处断裂的地震活动性弱.     

Effect of non-linear soil deformation on the interaction among energy piles

This study investigates the effect of non-linear soil deformation on the displacement interaction among energy piles. The work is based on interaction factor analyses of full-scale pile group tests, whose results are compared with experimental evidence. The results presented highlight the tendency of interaction factor analyses that ignore non-linear soil deformation to overestimate the interaction and the displacement of energy pile groups. This outcome, in accordance with previous studies for conventional pile groups subjected to mechanical loads, may be considered in the analysis and design of energy pile groups subjected to thermal (and mechanical) loads through the interaction factor method.