Spatial variability in growth and reproduction of the Pacific oyster Crassostrea gigas (Thunberg, 1793) along the west European coast

The Pacific oyster Crassostrea gigas was introduced in Europe for commercial purposes in the mid 1960s. It was initially thought that low winter temperatures would restrain this species' reproduction and settlement; however, its present distribution in areas where no introduction has taken place suggests that natural invasion and expansion has occurred. Along the European coast, wild populations of Pacific oysters are already found from northern Germany to southern Portugal. Whether C. gigas will continue to further expand through northern waters will depend on its physiological performance. In this study, the performance of wild oyster populations has been studied in terms of growth and reproduction at three stations: La Rochelle (France; 46°N), Yerseke (Oosterschelde estuary, The Netherlands, 51°N), and Texel (Wadden Sea estuary, The Netherlands, 53°N). The French population had the lowest somatic-shell mass ratio and an increase in maximum shell length, somatic and gonadal mass was observed from France to the Netherlands. In addition, mean oocyte diameter decreased significantly from south to north. The combination of increasing gonadal mass and decreasing oocyte volume suggests an increasing reproductive output in terms of egg numbers from France to The Netherlands. Differences in temperature between locations will at least be partly responsible for the observed patterns; however, other environmental factors (such as food availability, predation pressure, sediment type and/or seston concentration) cannot be excluded. Since smaller eggs (oocytes) are thought to have a longer development time, the environmental conditions along the Dutch coast may result in increased larval dispersal and possibly in further population expansion.     

Application of Atomic Force Microscopy to Observation of Marine Bacteria

The atomic force microscopy (AFM) is a recently developed, bench-top instrument that can image the surface structures of biological specimens at high resolution with simultaneous measurement of their size. This paper describes the application of AFM to marine bacteria. Both natural and cultured bacteria were retained on a filter or placed on glass, washed, air-dried and observed by AFM. The instrumental condition, the choice of suitable filter, effect of fixation and filtration, comparison with epifluorescent microscopic (EFM) count, and the size and shape of bacterial cells were investigated. An Isopore filter was best for concentration and subsequent observation because of its surface flatness. Cross section images showed that both rod and coccoid cells were flattened, the former usually having a two-humped shape. Bacterial cells were differentiated from non-living particles based on their cross section shape and size. Bacterial counts by AFM and EFM showed good agreement. Although size measurement is easily done by the instrument, AFM tends to overestimate the size of microspheres. More work is thus needed on the size measurement of living organisms. Because AFM easily provides images of natural bacterial cells at high magnification, it can be used as a new tool to study the fine structures of marine bacteria. This revised version was published online in July 2006 with corrections to the Cover Date.     

南海东北部末次冰期以来的沉积环境演变

利用南海东北部上陆坡处所取的D孔柱样进行硅藻分析,结合粒度与碎屑矿物分析结果,探讨该海域末次冰期以来沉积环境演变过程.结果表明：粒度与硅藻分析结果较为吻合,共同显示D孔柱样可分为上下两层.上表层（0～2 cm）的砂质沉积层为冰后期海进过程改造的晚更新世残留沉积层,即变余沉积;下层（2～130 cm）主要是末次冰期形成的浅海沉积.整个柱样可反映出海洋同位素MIS 4期海退至MIS 3期海进与MIS 2期海退至冰后期海进的沉积变化韵律.     

西太平洋暖池区热带波动的天气效应

为了探讨西太平洋暧地区热带波动的天气效应，利用1980年2－9月140°E日本静止卫星纬度时间剖面卫星云图，分析了5d和14d左右周期热带波动云的演变特征，井综合分析了14d周期的云系演变型式与流场的关系，为预报热带中期天气变化规律提供了依据；热带波动中30－60d大气低频振荡的云量变化最显著，北半球夏半年热带波动的天气压主要在10°N－0°，各半年在10°N－10°S，超过这个区域热带云量的港分布型式就有明显的变化。     

Experimental study of the breaking limit of multi-directional random waves passing over an impermeable submerged breakwater

This study investigates experimentally the breaking wave height of multi-directional random waves passing over an impermeable submerged breakwater. Experiments have been conducted in a three-dimensional wave basin equipped with a multi-directional random wave generator. A special type of wave gauge has been newly devised to record the water surface elevations in the breaker zone as accurately as possible. The records are analyzed to estimate the location and limit of wave breaking. Comparisons have also been made with the results of regular waves. The influence of the incident wave conditions on the breaking wave height normalized by the breakwater dimensions has been investigated. Empirical formulae have been presented to estimate the breaking limit of multi-directional random waves based on the experimental records. The formulae have been tested and found to work well not only for multi-directional random waves, but for regular waves as well.     

Regression Formulations for the Stress at Hot Spot of Multiplanar Tubular DT Joints

- Stress concentration analysis of multiplanar tubular DT joints plays an important role in the fatigue design of offshore platforms. A semi-analytic method for stress analysis under the condition of any loads is briefly introduced in the paper. Nineteen multiplanar tubular DT joints with one of two braces of the same dimension subjected to axial loads and out- of- plane bending moments are computed for parametric stress analysis by using the present method. The influence of geometrical parameters on the stresses of multiplanar tubular DT joints is discussed and compared with corresponding uniplanar T joints. The regression formulae for the stress at hot spot of multiplanar DT joints are found by modification of SCF of corresponding uniplanar T joints. The parametric formulae for the maximum stress by superposition. Finally, the influences of stiffening effect and load-interaction effect on the maximum stress of DT joints are discussed.