斑鳢(Channa maculata)微囊藻毒素去毒相关基因sGST的克隆与序列分析

采用RT-PCR技术和RACE技术成功克隆淡水鱼类斑鳢sGST基因cDNA全序列,推测得到氨基酸序列,初步分析其结构功能域及系统进化关系。结果表明,斑鳢sGST基因cDNA序列全长为898bp,编码225个氨基酸。斑鳢sGST与真鲷、金头鲷、鲽、黑头鲦、川鲽、大口黑鲈等最新定名为ρ型的sGST氨基酸同源性较高,ρ型sGST为水生生物所特有并共同占据进化树上独立的分枝;与大鼠、小鼠、人等哺乳动物sGST现有所有类型同源性均很低,并且在进化树上距离也较远,表明本研究成功克隆的sGST基因应属于ρ型,可能在鱼类等水生生物对水栖环境的适应上有重要作用。     

Elemental geochemical records of seafloor hydrothermal activities in the sediments from the Okinawa Trough

The major and minor element contents in the sediment core H9 from the hydrothermal fields of the Okinawa Trough show a sharp change at the depth of 80 cm. The elements enriched in the upper 80 cm core are those enriched in the hydrothermal deposits and in the surface sediments recovered from the hydrothermal fields in the trough, which indicates the input of hydrothermal materials. Comparing with other hydrothermal sediments from Mid-ocean Ridges or the Lau Basin, the degree of the enrichment of elements iron, copper,cobalt, and nickel is relatively low. However, the enrichment of elements manganese, lead, arsenic, antimony and mercury is remarkable. The average contents of these elements in the upper 80 cm core sediments are three to six times those in the lower section, and 3~12 times those in the surface sediments which are not influenced by hydrothermal activities. Hydrothermal activities have contributed significant manganese, lead, arsenic, antimony and mercury to the sediments, and these elements are distinct indicators for the hydrothermal activity in the Okinawa Trough. The significant enrichment of these elements in Core H9 upward from the depth 80 cm indicates the start or the significant enhancing of the hydrothermal activity in this area at about 5 740 aB.P. The average accumulation rate of manganese during this period is about 40 461 μg/(cm2·ka), which is similar to the hydrothermal sediments in the Lau Basin or the East Pacific Rise.     

南黄海表层沉积物中氮的区域地球化学特征

通过分级浸取分离的方法获得了南黄海表层沉积物不同形态的氮，首次探讨其分布的区域性特征：南黄海表层沉积物中各形态氮在不同测线上均呈现出波状分布。B测线的基本上呈现出中部海域高，两边海域低，而西部海域又略高于东部海域的趋势，D和E测线的基本上呈现出西高、东低的趋势，C测线的则呈现出西低、东高的趋势，F测线的变化趋势比较平缓，从整体上看，SOEF—N和TN的分布呈现出西部海区比东部海区低的趋势。受各测线上沉积物种类差异的影响，自北向南各形态氮的变化幅度逐渐减小，其中SOEF-N在各测线上的变化幅度最大，且自北向南降幅最大。受水动力及沉积物粒度的影响，不同形态氮在黄海槽内各站位沉积物中的含量较高。沉积物中各形态氮在不同测线上的分布还表明，陆源输入对南黄海沉积物中氮测线分布有较小的影响，海洋自生氮对南黄海表层沉积物中氮的形态、含量与分布具有重要影响。     

基于GIS数据仓库的激光单分子海洋油气化探数据可视化处理

采用基于地理信息系统(GIS)的数据仓库技术,以激光单分子海洋油气化探数据处理与评价作为数据仓库主题,实现了激光单分子海洋油气化探数据的可视化处理。将可视化处理分为4个步骤:数据预处理、异常背景分析、异常分析、异常综合评价;使用MAPX、DELPHI等软件研制开发了相应的可视化软件模块。通过对渤海湾盆地某测区实测的甲苯化探数据的处理,验证了所开发技术的实用性。     

Summer distribution and geochemical composition of suspended-particulate matter in the east china sea

The distribution and geochemical composition of suspended-particulate matter (SPM) in the East China Sea (ECS) were investigated during the summer period of high continental runoff to elucidate SPM sources, distribution and cross-shelf transport. The spatial variability of SPM distribution (0.3–6.5 mg l⁻¹) and geochemical composition (POC, Al, Si, Fe, Mn, Ca, Mg and K) in the ECS was pronounced during summer when the continental fluxes of freshwater and terrestrial materials were highest during the year. Under the influences of Changjiang runoff, Kuroshio intrusion, surface production and bottom resuspension, the distribution generally showed strong gradients decreasing seaward for both biogenic and lithogenic materials. Particulate organic carbon was enriched in surface water (mean ∼18%) due to the influence of biological productivity, and was diluted by resuspended and/or laterally-transported materials in bottom water (mean 9.4%). The abundance of lithogenic elements (Al, Si, Fe, Mn) increased toward the bottom, and the distribution correlations were highly significant. Particulate CaCO₃ distribution provided evidence that the SPM of the bottom water in the northern part of the study area was likely mixed with sediments originally derived from Huanghe. A distinct benthic nepheloid layer (BNL) was present in all seaward transects of the ECS shelf. Sediment resuspension may be caused by tidal fluctuation and other forcing and be regarded as the principal agent in the formation of BNL. This BNL was likely responsible for the transport of biogenic and lithogenic particles across or along the ECS shelf. Total inventories of SPM, POC and PN are 46, 2.8 and 0.4 Tg, respectively, measured over the total area of 0.45 × 10⁶ km² of the ECS shelf. Their mean residence times are about 27, 13 and 11 days, respectively. The inventory of SPM in the water column was higher in the northernmost and southernmost transects and lower in the middle transects, reflecting the influences of terrestrial inputs from Changjiang and/or resuspended materials from Huanghe deposits in the north and perhaps from Minjiang and/or Taiwan’s rivers in the south. The distribution and transport patterns of SPM and geochemical elements strongly indicate that continental sources and cross-shelf transport modulate ECS particulate matter in summer.     

海底天然气水合物地球化学探测技术

海底天然气水合物是未来的新型能源，地球化学探测与分析技术在天然气水合物勘探、研究和开发中发挥巨大作用。简要介绍了天然气水合物地球化学探测方法及相关的分析测试技术，包括海底沉积物、海水、海面低层大气中烃类气体(主要为甲烷)、孔隙水中阴阳离子和同位素地球化学异常等。并对发展天然气水舍物地球化学探测与分析新技术提出建议。     

火山热液型铅锌矿床岩石地球化学特征及预测指标

火山热液型铅锌矿床赋矿围岩、后期脉岩和蚀变岩石中成矿元素铅锌银含量高,矿体元素组合复杂,原生异常发育,并存在着原生分带现象。矿体前缘元素为I、Hg、As、Sb、B、Ba;近矿指示元素是Pb、Zn、Ag、Au、Cd、Mn、Cu;尾部或矿下指示元素为Cu、Mo、W、Sn。根据矿床指示元素的分布规律和原生晕的分带性,研制了判别矿床剥蚀程度和评价异常的地球化学指标,建立了该类矿床的地球化学找矿预测标志。     

上宫构造蚀变岩型金矿床地质特征及化探找金经验

上宫金矿位于河南省西部熊耳山北麓,在该区1 020 km²范围内进行了1:5万的地球化学水系沉积物测量,按水系划分取样单元,取样本着"小沟多取,支流放稀,大河不管"的原则进行。本次水系沉积物测量的特点是:取样少,控制面积大,速度快,省钱、省人、省物,找矿效果好、经济效益好。本区水系沉积物测量中所发现的金异常,后经异常检查、地质普查评价及钻探验证,确定为一大型构造蚀变岩型金矿床。     

商城县汤家坪钼矿地球化学特征及找矿远景

矿区地质、地球化学特征研究入手,总结了综合找矿标志,指出了进一步找矿方向。研究表明该矿床成矿组分单一,异常元素组合主要为Mo、Bi、Ag,次为W、Sn、Zn、Cu、Pb,仅有钼能形成较大规模的异常;矿床元素水平分带序列为(Mo-Bi-Ag)-(W-Sn)-(Pb-Zn-Cu),垂向分带序列为(Mo-Ag-Pb-Zn)-Cu-(Bi-W-Sn),并以w(Mo)/[w(Bi)×100]≥65,[w(Pb)×w(Zn)/w(W)×w(Sn)]≥25为判别标志,来预测深部盲矿体的存在。     

基于传统吸附烃概念的烃类检测新技术

烃类是地表油气地球化学勘查地下油气藏的直接指标.目前我国油气化探中常用的酸解烃法检测出的烃类,不应定义为"吸附烃",它是介质吸收的产物,属结合态烃类,应定义为"吸收烃".地表酸解烃异常十分稳定,重现性好,能够很好地证明地质历史上曾经有过的烃类运移过程,但在反映目前深部油气信息方面具有多解性.针对酸解烃法的弱点,依据烃类垂向运移理论和物质吸附作用原理,自行设计开发、研制了一套吸附烃脱气装置,建立了土壤吸附烃提取新技术和检测方法.该方法有利于在近地表获取油气系统的动态信息,强化烃类测量法对其信息源的探测,可以提高地表油气化探预测油气分布的成功率.