Molecular ecological study of <Emphasis Type="Italic">Siganus spinus</Emphasis> and <Emphasis Type="Italic">S. guttatus</Emphasis> from Okinawan waters based on mitochondrial DNA control region sequences

The mitochondrial DNA (mtDNA) control region was sequenced to examine the genetic variability and gene flow of juvenile Siganus spinus and S. guttatus. In total, 461 nucleotide sequences were obtained from 69 specimens of juvenile S. spinus from Okinawa Island and Ishigaki Island, in which 17 variable sites and 22 haplotypes were identified. Haplotype diversity (h) was high (0.9244 in Okinawa and 0.8984 in Ishigaki), whereas nucleotide diversity (π) was low (0.0063 in Okinawa and 0.0059 in Ishigaki). The two populations were not genetically distinct. Siganus guttatus, which do not form large schools at recruitment, in contrast S. spinus, were also analyzed by studying 152 individuals collected off Okinawa Island, Miyako Island, and Ishigaki Island. Of 476 nucleotide sequences, 50 were variable, and 42 haplotypes were identified. Genetic variability values were high (h = 0.8766 and π = 0.0151 in Okinawa; h = 0.9640 and π = 0.0192 in Miyako; h = 0.9161 and π = 0.0199 in Ishigaki). The Okinawa population was genetically isolated from the Miyako and Ishigaki populations. As a result, genetic diversity was high for each of these siganid populations despite their being target species for fisheries; however, the degree of inter-population gene flow was higher for S. spinus than S. guttatus, suggesting that these species exhibit different dispersal strategies.     

五个短蛸群体等位基因酶的遗传变异

采用水平淀粉凝胶电泳技术研究了山东沿海莱州（LZ），烟台（YT）、青岛（QD），日照（RZ）和大连（DL）五地短蛸自然群体的等位基因酶遗传变异。分析了AAT，ALP，EST，GRS，G6PD，SDH，CAT，SOD，IDH，GPI，PGM，G3PD，MPI，ME，MDH，CK，AK等同工酶在短蛸外套肌和肝脏组织中的表达情况，并对同工酶进行了生化遗传分析，LZ，YT，QD，RZ，DL五个群体的多态位点比例分别为15％，5％，10%,10%,10%(P0．99），群体平均杂合度观测值分别为0．024，0．001，0．009，0．007，0．006，平均位点有效等位基因数分别为1．200，1．022，1．061，1．103，1．077。结果表明，LZ群体的遗传多样性较高，同时，各群体中普遍存在杂合子缺失现象，对五个群体的遗传距离进行聚类分析，构建了系统树图。     

中国沿海星虫动物门名录

取1980～1989年间在我国沿海进行星虫动物区系调查的标本和有关资料做了整理、鉴定,共39种,分隶于2纲,4目,6科,12属。其中1种在我国为首次记录。     

Genetic difference of Chinese horseshoe crab (Tachypleus tridentatus) in southeast coast of China based on mitochondrial COI gene analysis

Population genetic structure and historical demography of Chinese horseshoe crab (T. tridentatus) along southeast coast of China were inferred from the sequence data of mitochondrial cytochrome c oxidase subunit I (COI) fragment. The sequence analysis for 964 bp COI fragment was conducted in 28 individuals collected from five localities: Ninghai in Zhejiang Province, Meizhou and Zhangpu in Fujian Province, Beihai of Guangxi Zhuang Autonomous Region and Danzhou of Hainan Province. Sequence variation was relatively low with a total of seven transitions observed. In all localities, Haplotype H3 was the dominant type observed among eight haplotypes defined previously, and was at the center of radiation in Median-Joining network. The prolonged star-like network suggests a signature of population expansions. The level of diversity was low in total, with haplotype diversity (H_d) being equal to 0.765 and nucleotide diversity (π) being equal to 0.001 18, respectively. The genetic structure analysis revealed the significant genetic difference between Ninghai and Danzhou populations. Both mismatch distribution analysis and Fu's F_s test provided consistent inference of historic population expansion. The low genetic diversity of horseshoe crab observed along China coast indicated that urgent measures should be taken to protect this rare marine animal.     

海水养殖鱼类细菌性疾病研究概况

本文概述性地介绍了发生在海水养殖中的常见细菌性疾病包括弧菌现、链球菌症、卡诺氏菌症、细菌性肾病、巴斯德氏杀菌症、爱德华氏症、滑走细菌症和杀鲑气单胞菌症等的主要症状、流行情况及病原菌特征，通过遗传育种筛选有抗病力的养殖品种是人们正在努力的方向，目前的研究重点主要在鱼类抗病能力方差分析研究及抗病育种的方法研究，免疫接种是提高养殖鱼类对某一特定性病原菌的最有效的方法，目前已有三十多种菌苗上市，通过使用免疫刺激剂提高鱼类的非特异性免疫力已显示一定的应用前景，虽然抗菌素由于存在抗药菌株和鱼体残留，但它仍是目前对细菌性疾病疾病的常规疗法，而海洋藻类提取物对细菌的抑制作用已被人们所认识，它将会成为一种有潜力的抗菌药物。     

场地效应研究的传递台站线性反演法——以芦山地震为例

采用线性反演法研究了地震动的场地效应,并针对线性反演法的局限性,提出了“传递台站”的方法来扩展其应用.通过传递台站,地震事件不再局限于必须被参考台记录到,台站也不再局限于须与参考台站同时获取到地震记录,改进后可大大增加数据样本,使得结果更趋稳定.同时,对于原来难以参与分析的台站,也可得到其场地反应.研究中,还采用了基于遗传算法的非参考台方法,进一步分析了场地反应和验证所提方法的合理性.将所提方法应用于2013年芦山7.0级地震周边区域,分析了这一地区的强震台站的场地反应和地震波衰减因子Q_s值.研究结果显示,当采用一个和二个传递台站进行分析时,所能利用的地震事件和所能求解场地反应的台站数目逐步增加,随着数据样本的增加,分析结果趋于稳定.研究区域的Q_s值拟合结果为Q_s（f）=334.16f^0.73.遗传算法结果表明,线性反演法中所选取的参考台站有其自身的场地反应.当利用遗传算法获得的参考场的场地反应去校正线性反演法的结果时,对于多数场地,两者的结果符合得很好,说明了遗传算法的结果可以合理地认为是场地较为真实的反应.

     

抗震设计反应谱特征周期研究

搜集整理1933—2015年美国西部地区166个有详细钻孔资料台站场地上的1 237条水平方向强震记录,按照我国建筑抗震设计规范(GB 50011-2010)中反应谱的形状对强震记录的加速度反应谱进行拟合,提取反应谱的特征周期。分析特征周期随场地类别、震级、震中距的变化特征;按特征周期分区统计计算不同场地类别上的特征周期平均值,并与我国现行的建筑抗震设计规范中给定的特征周期值进行对比研究;最后探讨地震动峰值加速度对特征周期的影响。根据研究分析结果提出关于抗震设计反应谱特征周期值的改进建议。     

Hierarchically nested river landform sequences. Part 2: Bankfull channel morphodynamics governed by valley nesting structure

River corridors exhibit landforms nested within landforms repeatedly down spatial scales. In Pasternack et al. ( 2018 ), a new, scale‐independent, hierarchical river classification was developed that uses five landform types to map the domains of a single fluvial process – flow convergence routing – at each of three–five spatial scales. Given those methods, this study investigated the details of how flow convergence routing organizes nested landform sequences. The method involved analyzing landform abundance, sequencing, and hierarchical nesting along the 35 km gravel/cobble lower Yuba River in California. Independent testing of flow convergence routing found that hydraulic patterns at every flow matched the essential predictions from classification, substantiating the process–morphology link. River width and bed elevation sequences exhibit large, nonrandom, and linked oscillations structured to preferentially yield wide bars and constricted pools at base flow and bankfull flow. At a flow of 8.44 times bankfull, there is still an abundance of wide bar and constricted pool landforms, but larger topographic drivers also yield an abundance of nozzle and oversized landforms. The nested structure of flow convergence routing landforms reveals that base flow and bankfull landforms are nested together within specific floodprone valley landform types, and these landform types control channel morphodynamics during moderate to large floods. As a result, this study calls into question the prevailing theory that the bankfull channel of a gravel/cobble river is controlled by in‐channel, bankfull, and/or small flood flows. Such flows may initiate sediment transport, but they are too small to control landform organization in a gravel/cobble river with topographic complexity. Copyright © 2018 John Wiley & Sons, Ltd.     

分区分类的生命线工程地震直接经济损失研究

生命线工程的震害及损失评估工作对于震害防御和震后地震应急工作有着极其重要的意义。 但是, 生命线工程是一个复杂庞大的网络系统, 目前对其开展的震害及损失评估研究工作存在着很大的局限性和不确定性。 本文充分利用研究较多且较系统的建筑物震害及损失评估结果, 结合历次典型历史地震震害中建筑物和生命线震害情况, 通过分析建筑物损失和生命线工程损失的数量关系, 建立二者之间的关联模型; 通过对人口、 GDP、 土地利用等公里网格数据进行分析, 给出中国大陆地区的分区分类原则, 建立分区分类的生命线工程地震直接经济损失分析模型; 基于GIS软件平台, 开发了生命线工程地震直接经济损失分析模块, 利用该模块, 得出了四川省不同地震烈度下的生命线工程直接经济损失空间分布情况。     

Soil characteristics in Doon Valley (north west Himalaya,India) by inversion of H/V spectral ratios from ambient noise measurements

Past and recent observations have shown that the local site conditions significantly affect the behavior of seismic waves and its potential to cause destructive earthquakes. Thus, seismic microzonation studies have become crucial for seismic hazard assessment, providing local soil characteristics that can help to evaluate the possible seismic effects. Among the different methods used for estimating the soil characteristics, the ones based on ambient noise measurements, such as the H/V technique, become a cheap, non-invasive and successful way for evaluating the soil properties along a studied area.In this work, ambient noise measurements were taken at 240 sites around the Doon Valley, India, in order to characterize the sediment deposits. First, the H/V analysis has been carried out to estimate the resonant frequencies along the valley. Subsequently, some of this H/V results have been inverted, using the neighborhood algorithm and the available geotechnical information, in order to provide an estimation of the S-wave velocity profiles at the studied sites.Using all these information, we have characterized the sedimentary deposits in different areas of the Doon Valley, providing the resonant frequency, the soil thickness, the mean S-wave velocity of the sediments, and the mean S-wave velocity in the uppermost 30 m.