中国花鲈细胞色素b基因片段的序列研究(英文)

参考鳗鲡等鱼类线粒体 DNA序列进行了中国花鲈线粒体 DNA细胞色素 b基因片断的引物设计、PCR扩增及其序列测定。得到中国花鲈的碱基序列为 4 10 bp,其 A、T、G、C含量分别为 10 1bp(2 4 .6 3% )、112 bp(2 7.32 % )、72 bp(17.56 % )、12 5bp(30 .4 9% ) ,与鳗鲡等其他鱼类相同基因片断序列碱基含量相似。     

Application of the first internal transcribed spacer(ITS-1) of ribosomal DNA as a molecular marker to population analysis in farrer''s scallop Chlamys farreri

Sequence variation of the first internal transcribed spacer of ribosomal DNA （ ITS - 1 ） was examined and its application to the study of genetic variation was explored in four populations of farter＇ s scallop Chlamys farreri. ITS - 1 fragments, with a length of about 300 bp,of 78 individuals collected from Dalian, Qingdao, Yantai in China and Korea respectively were amplified via PCR, cloned and sequenced. Intra-genomic variation was examined by sequencing several clones of single individuals. Alignment and polymorphism analysis detected 44 haplotypes and 50 polymorphic sites which consist of 30 substitutions and 20 indels, indicating a high level of polymorphisms. Sequence analysis also showed a very low level of intra-individual variation. All these features validated the feasibility of application of ITS - 1 fragment to population analysis. Polymorphism analysis showed that the Korea sample has the richest genetic variation, followed by Yantai and Qingdao samples. AMOVA （analysis of molecular variance） showed that the majority （96.26%） of genetic variation was distributed within populations and 3.74% resulted from among populations, but with P 〈 0.05 （ = 0.042）, indicating that the populations in this study have significant divergence. This output was basically concordant with the result arising from RAPD data and different from that from mitochondrial 16S rDNA sequence data. Discussion on this inconsistency was made accordingly.     

西沙礁序列的磁性地层学研究

利用西沙群岛西琛一井３３０ｍ以上井段的１４９块岩心标本，用美国２－Ｇ型超导磁力仪进行了古地磁测定，获得了一个从更新世至中新世晚期包括布容，松山，高斯和吉尔伯特四个极性时及若干亚极性的磁性地层柱，为礁序列的划分对比，提供了新的依据。     

闽江口第四纪地层中的孢粉、有孔虫、硅藻组合及其古地理意义

本文通过对闽江口琅岐岛钻孔的孢粉,有孔虫及硅藻的综合分析,发现第四纪地层中有五个孢粉组合带,五个硅藻组合带与一个微体化石层。结合~(14)C测年资料与沉积物特征认为该地区第四纪地层可以划分为晚更新世晚期与全新世(早期、中期、晚期)。气候演化依次为:冷干→温暖略干→温暖湿润→炎热潮湿→温暖略干。沉积相发展顺序为:冲积相、冲洪积相→河流相、湖沼相→湖沼相、河口相→海湾相→河口相。     

Genetic relationship between Neobenedenia girellae and N. melleni inferred from 28S rRNA sequences

The fragments of 350 bp in 28S rRNA from the closely related monogenea of trematoda, Neobenedenia girellae and N. melleni are obtained by polymerase chain reaction (PCR) amplified using a couple of special primers and then sequenced. The results show that the comparison of 28S rRNA sequences, with only a base varying in 337 bp accounting for 0.3% genetic difference, from the relative species N. girellae and N. melleni parasitized on the different fishes in different farms displays that they possess a very high genetic similarity of 99.7%, higher than that of 99.41% for the single species N. melleni sampled in different areas, and the intraspecific divergence of N.melleni is 0.59%. Meanwhile, the interspecific differences between the two Neobenedenia and three Benedenia (i.e., B. lutjani, B. rohdei and B. seriolae) range from 2.08% tol 1.73%. In addition, UPGMA and MP molecular phylogenetic trees are constructed and proved to be consistent with each other. Though the morphological characteristics and the results of genetic diversity for the two Neobenedenia show a high similarity, whether they belong to a single species or not are still undefined, and the more genes of them should be further investigated, in combination with the systematical and detailed morphological study.     

Main Controlling Factors on Hydrocarbon Accumulation and Distribution in Marine Sedimentary Sequences in South China

Multiple source rock assemblages were deposited in the sedimentary provinces in South China in geologic history,and some of them were destructed by and some survived against multiple tectonic movements.Therefore,multiple sources,mixed sources,and uneven distribution of sources occurred in the marine sedimentary basins in South China during the late stage of hydrocarbon pooling.Epidiagenesis of the marine carbonate reservoirs and its modification to reservoir poroperm characteristics determined the formation and the scale of natural gas pools.The exploration practices show that the large to medium gas fields mainly occur in areas with high-quality reservoirs.Detailed study of the paleo-oil accumulations and typical oil and gas reservoirs reveals that the basins experienced multiphase superimposition and modification,leading to the distribution of the Paleozoic paleo-oil accumulations and bitumen in the peripheral areas.The phenomenon that oil and gas production concentrates in the Sichuan basin indicates that the overall sealing conditions of a basin determine the oil/gas potentials and the scale of oil and gas production.This is a critical factor controlling the accumulation and distribution of gas in the marine sequences in South China.The early oil and gas pools in the Yangtze platform left billions of bitumen in the peripheral areas due to the destruction of seals.Since the Himalayan,"late-generation and late-accumulation" gas pools represented by the gas pools in the Sichuan (四川) basin were formed in the marine sedimentary sequences in South China as a result of the change of the sealing conditions.Current gas discoveries appear to be "paleo-generation and paleo-accumulation" gas pools but actually are "late-generation and late-accumulation" gas pools.These patterns of hydrocarbon pooling clearly depict themselves in western Sichuan basin and Weiyuan (威远)gas field.It is revealed that the gas pools in the Sichuan basin were mainly formed as a result of hydrocarbon phase change (thermal cracking of oil to gas),miscible migration,and dynamic equilibration since the Himalayan.A large number of gas pools were formed in the Himalayan and the gas pools in the marine sequences are characterized by late pooling; this kind of gas fields/pools are controlled by:(1) effectiveness of modification and superimposition of the marine basins,(2) effectiveness of the source rocks,(3) effectiveness of the overall preservation conditions,and (4) effectiveness of plays.     

基于钻孔数据的三维地质建模与可视化研究

三维地质模型能够完整准确地表达复杂地质现象的边界条件及地质体内含的各种地质构造,直观地再现地质单元的空间展布及其相互关系,最大限度地提高地质分析的直观性和准确性,因此三维地质建模已引起地质、采矿、岩土工程等诸多领域的日益重视。笔者在综合考察多种建模方法的基础上,结合工程实践,提出了一种实用的工程地质三维建模实施方法。该方法以钻孔资料和地质剖面数据为三维地质建模的源数据,允许用户手工修正模型剖面地层分界线,从而实现对建模过程的干预和控制,克服了单纯依靠钻孔数据建模结果不精确且难以修正的问题。最后,通过一个研究实例展示了该方法的实际建模效果。     

化学地层学研究中的若干注意事项

化学地层学因其交叉学科特点和研究对象的复杂性使得研究过程中任何一个环节的不足都将造成测试出现偏差,成果的可信度也将出现问题。高昂的成本也要求研究者把有限的资源集中到正确的目标上。近20年研究历程却恰恰将此类性质的问题陆续暴露了出来。如采样阶段获取原生性状考虑不充分或依据不足、测试阶段缺乏校验、数据分析阶段挖潜和综合利用有待完善以及化学岩、碎屑岩和生物岩各自的基础性和针对性研究有待提高等。有些问题带有相当程度的普遍性,有些甚至是严重的核心技术问题。笔者结合最新相关研究成果对上述问题进行了逐一分析和系统修正。     

Distribution of Palygorskite in the Lingtai Profile of Chinese Loess Plateau: Its Paleoclimatic Implications

Palygorskite is a typical indicator mineral of the arid and strong evaporation environment. Distribution of palygorskite in loess-red clay sequences may act as an important indicator for reconstruction of the paleoenvironment and paleoclimate. In this paper, field emission scanning electron microscope and high-resolution transmission electron microscope observations on the red clay-loess-paleosol of the Renjiapo profile at Lingtai, Gansu Province indicate that palygorskite occurs widely in red clay sequences formed before 3.6 Ma, but no occurrence has been found in eolian sediments since 3.2 Ma. Micromorphological features and microstructure of palygorskite show that it is an autogenic mineral formed during pedogenesis, and transformed from iilite-montmorilionite under the pore water action. In the Lingtai profile, the disappearance horizon of palygorskite is consistent with those of increasing magnetic susceptibility, dust flux and depositional rate. The distribution of palygorskite in the profile indicates that the interval of around 3.6 Ma was an important transformation period of the East Asian paleomonsoon, when changes took place in the East Asia paleoclimate pattern, i.e. a high-frequency strong fluctuation alternative evolution of the environment. Therefore, palygorskite is a key indicator mineral of the East Asian paleomonsoon evolution of that time.     

准噶尔盆地中拐地区下侏罗统层序地层特征与聚煤控制因素分析

在分析前人对准噶尔盆地层序地层学研究成果的基础上,综合应用岩心、测井和地震资料,对准噶尔盆地西北缘中拐地区下侏罗统进行层序地层划分,总结了层序和体系域界面的识别标志,将下侏罗统划分为4个三级层序和7个体系域,同时叙述了各三级层序的结构特征。通过对层序内含煤岩系的发育特点分析,发现其主力煤层主要发育在JSQI和JSQ3湖侵体系域中,本文作者认为研究区内湖侵时期为有利的聚煤时期,聚煤作用主要受构造沉降、物源供给、气候和沉积环境等因素的控制。