东海原甲藻cDNA文库构建及尝试性EST分析

东海原甲藻(Prorocentrumdonghaiense)是中国沿海频繁发生的大规模赤潮的原因种之一,大规模分离鉴定东海原甲藻功能基因是理解东海原甲藻赤潮形成过程和机理的重要基础。取对数生长期藻体,经微量总RNA抽提、cDNA合成、cDNA扩增和克隆等步骤,构建了东海原甲藻cDNA文库并进行了尝试性表达序列标签分析。从含有约5000个转化子的文库中随机取150个测序,获得126条EST序列。经网上BlastN及BlastX分析,共发现11个是已知功能的基因的标签。这些基因与东海原甲藻生长发育、物质转换和能量代谢相关。     

南黄海辐射沙脊群西洋潮流通道的浅部沉积层序及其形成演化再认识

晚第四纪以来,黄河、长江都曾经江苏中部海岸注入南黄海,河海交互作用形成一系列沉积,全新世海侵后发育岸外辐射沙脊群。沙脊群西北部、由岸滩与沙脊所夹持的西洋潮流通道,位于北侧废黄河三角洲和南侧长江三角洲两大地貌单元间的过渡区,成为揭示不同大河交互作用下的海岸、陆架晚第四纪沉积层序模式的重要窗口。最近通过更多晚第四纪钻孔对比和浅层地震剖面集成研究发现：① 由于混乱的测年结果和陆相硬黏土层对比不当,造成之前基于07SR01孔和Y1孔构建的辐射沙脊群西洋潮流通道浅部沉积（标高−60 m以内）的年代框架有误,其主体应是晚更新世沉积且发育两个沉积旋回,末次冰盛期硬黏土层多被潮流侵蚀而缺失,表层全新世沉积厚度在水下沙脊处基本不足10 m,其余普遍不足5 m,甚至缺失;② 仅在西洋西北段稳定分布的浅层地震单元U3指示了MIS 3古黄河三角洲的南缘,自晚更新世以来西洋所在的江苏中部海岸可能深受古黄河物源的影响,这尚需在西洋西北段的关键位置钻取新孔,并结合已有浅层地震剖面和东南段钻孔来进一步研究证实。提出下一步工作将基于层序地层学方法,通过对已有控制性浅层地震剖面进行地震层序格架的三维可视化、提取地震单元和反射界面的空间分布特征,结合已有及新增控制性钻孔的沉积学和年代学研究,构建可靠年代框架、判识大河物源,并参考邻区钻孔资料,来探明西洋潮流通道的浅部沉积层序,反演其形成演化。     

基于构造—地层学的隐蔽圈闭预测——以松南十屋断陷深层为例

综合运用高精度层序地层学、构造一地层学方法,认为十屋断陷深层发育的各类隐蔽圈闭是下一步勘探工作的重要目标。同时,由于构造对沉积的控制作用,在断陷内部不同的构造部位具有不同的隐蔽圈闭分布特征。十屋断陷西部皮家地区、北部张家屯一八屋地区位于油气主要运移指向区,分别由断裂坡折带和以挠曲型褶皱为特征的弯折带控制的储集砂体十分发育,盖层及保存条件也十分优越,是深部原生岩性、地层油气藏最有利的勘探目标区带。     

GIS专题数据综合的研究

随着GIS技术在各种应用领域的推广使用，各领域的专业数据与G1S的空间数据相结合，形成描述特定地理现象的各种专题数据。与GIS空间数据一样，专题数据的应用也由微观的、较为详细地描述小区域地理现象特征，转向宏观的、较为概略地描述较大范围地理现象特征，这是专题数据综合的主要任务。该文在讨论GIS专题数据类型及特征的基础上，着重分析了专题数据综合的基本类型，并提出了专题数据综合的概念模型，为GIS专题数据自动综合的实施提供理论依据和实施方案。     

闽江口外海域MIS 5和MIS 3的地震地层学特征和古环境

通过闽江口外海域进行的高分辨率浅地震剖面(拖筏)调查,揭示了MIS 6末期以来最大厚度约90 m的沉积地层。研究了MIS 5和MIS 3地震层序的反射波结构特征,基于地震相分析识别沉积地貌类型;结合深海氧同位素海平面变化曲线,阐述了两时期研究区的沉积环境、特殊地貌的分布和体系域阶段;最后简要探讨了海侵视强度(视高度)及其影响因素。在MIS 5,研究区主要为滨浅海环境,在MIS 5.3和MIS 5.1局部发育了赛江水下三角洲、潮流沙脊和滨海沙坝等沉积地貌;在初期和低海面亚期为陆相环境,发育了闽江和赛江河道,分别经南部和东北部向东入海(台湾海峡)。在MIS 3,研究区普遍为海水覆盖,以滨浅海环境为主,局部发育闽江水下三角洲叶瓣和潮流沙脊。MIS 5的海平面远高于MIS 3,但是典型地震剖面的研究表明,前者的海侵视强度(视高度)低于后者,推断这一现象主要与构造沉降有关,沉积物压缩也有一定的影响,这也可以解释南部海区MIS 5.2的古闽江河道要远低于当时海平面的现象。     

Population genetic diversity and structure of a dominant tropical seagrass,Cymodocea rotundata,in the Western Pacific region

Cymodocea rotundata is an ecologically important tropical pioneer seagrass species distributed in the Indo‐Pacific region. The population genetic diversity and structure of this species were analysed at 46 sites spanning the Philippines, Ryukyu Islands (northern limit) and Hainan Island, by using microsatellite simple sequence repeat (SSR) markers. Analyses revealed the persistence of C. rotundata likely relies on local population dynamics and fitness influenced by environmental gradients, with sexual reproduction prevalent in the Philippines while the Ryukyu and Hainan populations were predominantly established by clonal spread. Analysis of molecular variance showed significant genetic differentiation (P < 0.001) among three geographic regions: the Philippines, Ryukyu and Hainan. Furthermore, the mean fixation index value was very high (F_ST = 0.36), indicating poor dispersal potential or limited gene flow. Allelic richness and heterozygosity of C. rotundata was comparable in the Philippines and Ryukyu Islands populations. More private alleles were found in the Philippines and excess heterozygotes in the Ryukyu Islands. STRUCTURE analysis revealed that the Ryukyu and Hainan populations were mosaics of admixed alleles of individuals from the Philippines. An assignment test suggested that recruitment occurs from the Northeast Philippines to Ryukyu Islands. These results suggest that the Philippine populations are tropical seagrass hotspots and perhaps the origin of the populations in the Ryukyu and Hainan Islands. This scenario is most likely driven by the Kuroshio Current and island integration events during plate tectonic activities. The Philippine Archipelago is considered of high importance for conservation objectives and management plan of seagrasses. Likewise, the Ryukyu Islands are also important in terms of conserving regional diversity because locally adapted genotypes have important evolutionary potential in the face of environmental change.     

南黄海盆地中部隆起的形成与演化

南黄海盆地是在前震旦系克拉通基础上发育的中、古生界海相与中、新生界陆相多旋回叠合盆地。通过地震资料解释,结合邻区钻井与区域地质资料,对南黄海盆地中部隆起中、古生代地层及其形成演化进行了研究,结果表明,南黄海盆地中部隆起沉积了较全的中、古生界海相地层,发育第四系—新近系、中—下三叠统青龙组、上二叠统、下二叠统—上泥盆统、中—下志留统,奥陶系—震旦系和前震旦系变质岩系等7套地震地质层序;主要经历了前震旦纪基底形成、震旦纪—早古生代克拉通发育、晚古生代—中三叠世稳定台地—陆内裂陷、晚三叠世—古近纪形成与抬升剥蚀及新近纪-第四纪坳陷沉降5个阶段。     

复杂线-线对象的拓扑关系描述与计算方法

空间拓扑关系是GIS中空间查询和分析的基础。针对当前空间拓扑关系模型在表达较复杂对象间拓扑关系存在局限性的突出问题,以线对象为实例,根据点集拓扑理论,重新定义和区分线对象的复杂性;以9I模型为基础,提出一种适合二维复杂线对象的拓扑关系的线性序列描述模型,将复杂线-线的拓扑关系表示成基本拓扑关系的组合。分析不同情形下线之间拓扑关系不同的计算方法。为实现复杂线-线拓扑关系的计算,提高扫描线算法的效率,探讨包络矩形粗滤、线节点重合或共线的斜率坐标判断法等改进方法,提出判断线-线是否相交的矢量叉乘法,具有快速高效的特点。最后,通过实验系统导入线坐标串,进行图形绘制、拓扑关系计算并输出结果,从而验证该模型和算法的可行性。     

Adding the missing third and fourth dimensions to trajectory analysis in carbonate systems

We developed a seismic geomorphology-based procedure to enhance traditional trajectory analysis with the ability to visualize and quantify lateral variability along carbonate prograding-margin types (ramps and rimmed shelves) in 3D and 4D. This quantitative approach analysed the shelf break geometric evolution of the Oligo-Miocene carbonate clinoform system in the Browse Basin and delineated the feedback between antecedent topography and carbonate system response as controlling factor on shelf break rugosity. Our geometrical analysis identified a systematic shift in the large-scale average shelf break strike direction over a transect of 10 km from 62° to 55° in the Oligo-Miocene interval of the Browse Basin, which is likely controlled by far-field allogenic forcing from the Timor Trough collision zone. Plotting of 3D shelf break trajectories represents a convenient way to visualize the lateral variability in shelf break evolution. Shelf break trajectories that indicate contemporaneous along-strike progradation and retrogradation correlate with phases of autogenic slope system re-organization and may be a proxy for morphological stability of the shelf break. Shelf break rugosity and shelf break trajectory rugosity are not inherited parameters and antecedent topography does not dictate long-term differential movement of the shelf margin through successive depositional sequences. The autogenic carbonate system response to antecedent topography smooths high-rugosity areas by filling accommodation and maintains a relatively constant shelf break rugosity of ~150 m. Color-coding of the vertical component in the shelf break trajectory captures the creation and filling of accommodation, and highlights areas of the transect that are likely to yield inconsistent 2D sequence stratigraphic interpretations.     

廊固凹陷近岸水下扇特征及控制因素

通过对廊固凹陷18口井541 m岩心的系统观察、156口井测井序列的建立、1km×1 km三维地震测网密度的地震相识别,分析了该沉积体系的相带展布特征。该砂砾岩层总体趋势为向上厚度减薄、层数减少,共识别出5期扇体,多呈条带状沿大兴断层断面分布,每期之间发育稳定的暗色泥岩段。该近岸水下扇的形成受3级层序、大兴断层、古构造、古气候、古地形以及物源供给等多种因素的控制。3级层序的发育特点、大兴断层的分段活动以及特殊的物源供给背景因素的匹配对近岸水下扇形成起到决定性作用。2~3期扇体扇中分布范围广,储集条件好,是凹陷下一步开发工作的有利目标。