光谱增感染料的结构与其光谱吸收

增感染料在摄影、印刷制版等领域中的应用是十分广泛的，增感染料可以使感光材料的感光度提高，可以改善和扩大其感色性和画面层次等。增感染料的增感效率与其光吸收有着直接的关系，而增感染料的光吸收作用又受到其结构的影响。本文分析了增感染料的结构对其光谱吸收的影响。     

Comparative tracing experiments in a porous aquifer using bacteriophages and fluorescent dye on a test field located at Wilerwald (Switzerland) and simultaneously surveyed in detail on a local scale by radio-magneto-tellury (12–240 kHz)

This article presents an example of a tracing experiment using two bacteriophages, T7 and f1, and a fluorescent dye (naphthionate), in a saturated porous environment. The test field was equipped with an injection borehole and 22 sampling piezometers set in three concentric half-circles. The distribution of permeabilities and the thickness of the aquifer were indirectly determined by Radio-Magneto-Tellury (RMT, 12–240 kHz). The results reveal a good correlation between the distribution of permeabilities obtained by RMT and the breakthrough curves and speed of migration of all three tracers. The restitution levels are far superior (by two to three orders of magnitude) in the more permeable zones, as opposed to those observed in th piezometers situated in less permeable areas. The speed of migration of the biological tracers is much greater than that of the naphthionate. In the most extreme case, the T7 bacteriophage migrated about 3.15 times faster than the chemical solution. These results indicate that bacteriophages are able to travel considerable distances along permeable gravel channels. They may be used as biological tracers and as models for the migration of pathogenic viruses. The simultaneous use of tracing techniques and appropriate geophysical methods leads to a better knowledge of the hydrogeological parameters of the underground terrain. This combination allows for a better interpretation both of the speeds of migration and of the maximal concentrations of the tracers, and thus considerably increases the interpretability of hydrogeological impact studies.     

污水污泥对染料废水的吸附脱色性能研究

为探讨污泥的资源化利用和开发新型的染料废水吸附剂 ,在对污水污泥吸附处理染料废水的工艺条件进行优化的基础上 ,得到污水污泥对 3种染料 (中性橙、中性黑BL ,元青 )的最大吸附量及其吸附类型。结果表明 ,污泥焙烧温度、废水pH值和吸附时间对吸附效果有影响 ,而废水温度的变化则不会引起脱色率的较大改变。在最佳工艺条件 (污泥经 40 0℃焙烧 ,废水pH值为 1,吸附时间为 3 0min ,水温为 3 0℃ )下 ,3种染料废水的脱色率均达到 98% ,其最大吸附量都在 2 9mg·g-1左右 ,与活性炭的效果相当 ,而高于其它种类的吸附剂。焙烧后的污泥对中性黑BL的吸附符合Langmuir方程 ,对中性橙和元青的吸附符合Henry方程。焙烧污泥的优良脱色性能使其在染料废水处理方面具有较好的应用前景。     

Routing of canopy drip in the snowpack below a spruce crown

Snow that is retained by a forest canopy may either sublimate or evaporate directly from the crown or drop as snow clumps or meltwater to the ground. Redistributed snow and meltwater affect the snow structure and prevent the formation of mechanically weak layers, which is the prerequisite for avalanche formation in forests. In this paper we describe the results of dye tracer experiments conducted in a subalpine forest near Davos, Switzerland. Before a snowfall event we stained snow‐free branches of a spruce with a dye tracer solution. After snowfall the coloured meltwater dripping from the branches down on to the snowpack stained the percolation pathways of the meltwater in the snowpack. Photographs of the snow profiles indicate that the meltwater seeped almost vertically through the isothermal snowpack to the soil surface not exceeding the projected crown edge. Meltwater of different events moves along different preferential flow channels in the snow suggesting that old channels are not non‐conducting and additional meltwater fronts create new channels. Copyright © 1999 John Wiley & Sons, Ltd.     

Seasonal changes in the morphology of the subglacial drainage system,Haut Glacier d'Arolla,Switzerland

Dye tracing techniques were used to investigate the glacier-wide pattern of change in the englacial/subglacial drainage system of Haut Glacier d'Arolla during the ablation seasons of 1990 and 1991. Analysis of breakthrough curve characteristics indicate that over the course of a melt season, a system of major channels developed by headward growth at the expense of a hydraulically inefficient distributed system. By the end of the melt season, this channel system extended at least 3·3 km from the snout of the 4 km long glacier and drained the bulk of supraglacially derived meltwater passing through the glacier. The upper limit of the channel system closely followed the retreating snowline up-glacier. Rates of headward channel growth reached c. 65 m d⁻¹, although these rates decreased in the upper 1 km of the glacier where snowline retreat exposed a patchy firn aquifer. It appears that the removal of snow (with its high albedo and significant water storage capacity) from the glacier surface resulted in a dramatic increase in the volume of runoff into moulins, and in the peakedness of daily runoff cycles. This induced transient high water pressures within the distributed drainage system, which caused it to evolve rapidly into a channelised system. It is therefore likely that, at a local scale, channel growth occurred down-glacier from moulins, and that the overall up-glacier-directed pattern of channel formation was caused by the retreating snowline exposing new moulins and crevasses to inputs of ice-derived meltwater. Damping of diurnal melt inputs by storage in the firm aquifer accounts for the slowing of channel growth in the upper glacier. © 1998 John Wiley & Sons, Ltd.     

鄱阳湖换水周期与示踪剂传输时间特征的数值模拟

在复杂湖泊水动力环境作用下,换水周期和传输时间变化直接影响着污染物的迁移和转化.本文运用数值模拟方法,定量研究了季节水情动态下鄱阳湖换水周期和示踪剂传输时间的空间分布.结果表明,不同季节下鄱阳湖换水周期均具有较高的空间异质性,贯穿整个湖区的主河道换水周期约10 d,大多湖湾区的换水周期则长达300多天.尽管不同季节下换水周期空间分布格局几乎相似,但受鄱阳湖水动力场的季节变化影响,夏、秋季的换水周期要明显大于春、冬季.基于换水周期频率分布曲线的统计表明,80%的鄱阳湖区的换水周期约30 d,其余湖区换水周期为几十天至几百天,表明鄱阳湖应该更加确切地描述为一个快速换水和慢速换水同时共存的湖泊系统.鄱阳湖示踪剂传输时间介于4~32 d,夏、秋季的传输时间(11~32 d)约为春、冬季(4~8 d)的4倍,主要与鄱阳湖季节性水情特征及示踪剂的迁移路径有关.本文所获取的换水周期和示踪剂传输时间的时空分布信息可为今后鄱阳湖水质、水环境和生态系统管理和维护等方面提供重要科学参考.     

基于离散三维活体荧光光谱法的湖泊水体叶绿素a浓度原位测量

荧光光谱分析技术具有灵敏度高、检测快速等优点,三维荧光光谱提供的指纹荧光信息比普通荧光光谱更丰富,选择性更好,在多组分分离上更具优势.离散三维活体荧光光谱法通过提取水体蓝藻、绿藻、硅藻、甲藻和隐藻5个门类藻类荧光光谱的指纹特征,分类测量藻类叶绿素a浓度,经过加和得到总的水体叶绿素a浓度.将基于该方法研制的三维荧光光谱水体藻类原位测量仪用于太湖水体叶绿素a浓度的测定,并与YSI多参数水质检测仪、BBE藻类现场分析仪、分光光度法等测定结果对比,结果表明:该方法与分光光度法间无显著性差异,与分光光度法、BBE法间的相关性好,相关系数达0.96以上,精密度、准确率优于基于普通荧光法的原位测量仪,是一种快速有效的原位测量方法.     

GFP标记的迟缓爱德华菌感染诸氏鲻虾虎鱼后组织分布研究

为探讨迟缓爱德华菌(Edwarsiellatarda)入侵途径,建立感染模型,作者通过电转化法构建GFP标记的迟缓爱德华菌EtMc1512(质粒PMDpp-EGFP),实验设立浸泡组、腹腔注射组和肌肉注射组,感染后采集各组实验诸氏鲻虾虎鱼(Mugilogobius chulae)血液、鳃、肝脏、肠、肌肉,培养法统计分析各组织中的荧光细菌数;浸泡组取样时间为0、2、4、6、8、12、24 h,腹腔注射组和肌肉注射组取样时间为6、12、24、48、72、96h。结果显示,构建的EtMc1512-GFP具有较强荧光,GFP标记前后菌株毒力基因(citC、mukF、esrB、katB、fimA、gadB)检测结果均为阳性。浸泡感染后实验鱼各组织内的荧光菌随时间表现为先升后降的趋势,最高菌量出现在肠道(2.51×106CFU/g),其次为鳃(4.19×104CFU/g)、血液(1.65×104CFU/g),肠道荧光菌显著高于其他组织(P0.05);腹腔注射感染后肝脏(4.55×106CFU/g)和血液(4.65×106CFU/g)菌量最高;肌肉注射感染后肌肉在48h首先检出荧光菌,血液(2.93×104 CFU/g)菌量最高。结果表明,肠道、肝脏和肌肉分别是迟缓爱德华菌浸泡感染、腹腔注射感染和肌肉注射感染诸氏鲻虾虎鱼的主要组织器官,在自然条件下迟缓爱德华菌经口感染诸氏鲻虾虎鱼风险较高。     

海洋有机质的光谱分析方法评述

海洋有机质的光谱分析已成为海洋碳循环的研究热点之一。本文从天然有机质吸收光谱和荧光光谱分析的基本原理入手,系统梳理了有机质光谱分析中容易被忽视的基本概念,讨论了过滤与保存方式、p H值、荧光内滤效应等对有机质光谱分析的影响。结合色氨酸、酪氨酸、腐殖酸及大量现场样品的实验结果,对吸收光谱和三维荧光光谱的不同解析方法进行了系统的分析与对比。基于在南海、西菲律宾海获取的现场调查数据,对于文献中常用的荧光指数(FI)、腐殖化指数(HIX)、自生源指数(BIX)等光谱参数的内在含义及其在海洋环境中的适用性进行了评述。此外,还归纳和总结了胶体有机质和颗粒有机质荧光分析的最新进展,并对今后在光谱分析与观测领域有待完善和突破的一些关键领域进行了展望。