Estimating the mean speed of laminar overland flow using dye injection‐uncertainty on rough surfaces

A common method for estimating mean flow speeds in studies of surface runoff is to time the travel of a dye cloud across a measured flow path. Motion of the dye front reflects the surface flow speed, and a correction must be employed to derive a value for the profile mean speed, which is always lower. Whilst laminar flow conditions are widespread in the interrill zone, few data are available with which to establish the relationship linking surface and profile mean speeds, and there are virtually none for the flow range 100 < Re < 500 (Re = Reynolds number) which is studied here. In laboratory experiments on a glued sand board, mean flow speeds were estimated from both dye speeds and the volumetric flow relation v = Q/ wd with d measured using a computer‐controlled needle gauge at 64 points. In order to simulate conditions applicable to many dryland soils, the board was also roughened with plant litter and with ceramic tiles (to simulate surface stone cover). Results demonstrate that in the range 100 < Re < 500, there is no consistent relation between surface flow speeds and the profile mean. The mean relationship is v = 0·56 v _surf, which departs significantly from the theoretical smooth‐surface relation v = 0·67 v _surf, and exhibits a considerable scatter of values that show a dependence on flow depth. Given the inapplicability of any fixed conversion factor, and the dependence on flow depth, it is suggested that the use of dye timing as a method for estimating v be abandoned in favour of precision depth measurement and the use of the relation v = Q/ wd , at least within the laminar flow range tested. Copyright © 2001 John Wiley & Sons, Ltd.     

中国对虾弧菌病的间接荧光抗体诊断技术研究

于１９９４年８月－１９９５年５建立中国对虾病原菌－副溶血弧菌的间接荧光抗体检测技术，其中副溶血弧菌的特异抗血清由家兔制备，羊抗兔免疫球蛋白用异硫氰酸荧光素标记，并以罗丹明标占血清白蛋白为背影染色。     

应用亮兰染色剂指示溶质迁移边界层的研究

应用边界层方法确定溶质迁移参数是一种简单可靠的方法,但如何确定边界层的位置是限制边界层方法应用的关键因素。而亮兰染色剂在土壤中迁移时,具有边界层清晰、容易确定的特点。基于此,以Cl-为例,研究了该染色剂指示非反应性溶质迁移边界层的可行性。研究结果表明:在同一土柱中,亮兰染色剂的边界层与Cl-的边界层的运动规律相似,亮兰染色剂和Cl-的边界层随时间的变化在弥散度很小的情况下都可以用线性关系式近似描述。根据这一规律,得出在风沙土和黄绵土中Cl-边界层与亮兰染色剂边界层之间的关系表达式。两者边界层之间转换关系的求得,为应用亮兰染色剂准确指示非反应性溶质迁移边界层成为可能提供了一条思路。     

浒苔绿潮暴发期间海水溶解有机碳的生物可利用性及其碳汇作用

自2007年以来,浒苔绿潮已经连续15年在南黄海暴发。浒苔(Ulva prolifera)作为主要肇事藻种,在暴发过程中向海水释放大量的溶解有机碳(DOC)。然而,这些藻源DOC能否长期保存在海洋中,主要取决于它们的生物可利用性,目前关于此方面的研究甚少。本研究在浒苔绿潮大规模暴发时期(2019年6月),分别在浒苔暴发海区和无浒苔海区各选择3个站位富集表层海水,在实验室进行长期(300 d)的DOC降解实验。结果发现,在60 d内,不同站位富集海水中的DOC浓度随着微生物的利用快速下降,微生物丰度也在第60天达到峰值,表明这些被消耗的DOC是生物可利用性高的活性DOC(LDOC)。60 d后,剩余的DOC可抵抗微生物的降解,在60～300 d内保持稳定,表明这些DOC是具有强稳定性的惰性DOC(RDOC)。最终发现,浒苔暴发海水的RDOC占富集DOC的46%,明显高于无浒苔海水的(36%)。并且,荧光溶解有机物(FDOM)中活性的类蛋白组分随着微生物的利用被快速消耗,惰性的类腐殖质组分逐渐积累,暗示了在降解过程中LDOC逐渐向RDOC转化。可见,浒苔绿潮暴发除了在短时间内增加海水中的D...     

海洋荧光溶解有机物质的研究

海洋溶解有机物质参与海洋中的许多物理、化学和生物作用,对认识海洋环境中所发生的各种过程具有重要意义。作者对海洋溶解有机物中的荧光组分即荧光溶解有机物的种类、来源、分布变化及其地球化学行为进行较为系统的评述。海洋中其可能的来源包括陆源输入,浮游植物生长、代谢及分解产物,沉积物释放,降雨等。其主要的去除途径则是光化学降解。     

Determination of Trace Copper (Ⅱ) in Water Samples by Kinetic-spectrophotometry

A new kinetic-spectrophotometric method is proposed for the determination of copper ( Ⅱ ). The method is based on the catalytic effect of copper ( Ⅱ ) on the oxidation of weak acid brilliant blue dye (RAWL) by hydrogen peroxide. The copper ( Ⅱ ) can be determined spectrophotometrically by measuring the decrease of absorbance of RAWL at λ = 626 nm using the fix-time method. The optimum reaction conditions are as follows: pH 7.20, buffer solution NaOH-KH2PO4, RAWL (200 mgL-1) 5.00 mL, H2O2 (30％) 0.50 mL, reaction temperature 80 ℃ and reaction time 20 min. The linear range of this method is between 0 μg L-1 and 12 μg L-1 and the limit of detection is 0.011 μg L-1, the relative standard deviation (RSD) in five replicate determinations for 2 and 8 μg L-1 copper ( Ⅱ ) are 3.2％ and 2.3％, respectively. Twenty ions do not interfere in the determination of copper ( Ⅱ ). The method has been applied satisfactorily to the determination of copper ( Ⅱ ) in freshwater samples (tap water and Yellow River water from Lijin, Shandong, China) and seawater samples (from the South China Sea), the recovery rates are 98.0％, 102.5％ and 96.0％, respectively.     

The Effect of Oxygen on Anaerobic Color Removal of Azo Dye in a Sequencing Batch Reactor

In this study, various amounts of oxygen were added to the anaerobic phase of an anaerobic‐aerobic sequencing batch reactor (SBR) receiving azo dye remazol brilliant violet 5R to mimic the input of oxygen into the anaerobic zones of biological textile wastewater treatment plants. The effect of oxygen on the anaerobic biodegradative capability of the mixed microbial culture for remazol brilliant violet 5R was investigated. To investigate the effect of oxygen on anaerobic azo dye biodegradation, the anaerobic phase of the SBR cultures were exposed to a very low limited amount of oxygen for various air flow rates. Initially, an air flow rate of 20 mL/min was applied, further on the air flow rate in the anaerobic phase was increased up to 40 mL/min. System performance was determined by monitoring chemical oxygen demand, color removal rate, activities of anaerobic (azo reductase) and aerobic enzymes (catechol 2,3‐dioxygenase, catechol 1,2‐dioxygenase). The results of percentage COD reduction at each stage were similar for all runs, giving an overall reduction of 96%. Anaerobic color removal efficiency and azo reductase activity of anaerobic microorganisms were adversely affected by the addition of oxygen. Color removal efficiencies of the anaerobic phases decreased from 80% down to 42 and 38% for the limited oxygen conditions of 20 mL/min and 40 mL/min, respectively. It was observed that the activity of catechol 2,3‐dioxygenase and catechol 1,2‐dioxygenase, involved in breakage of aromatic rings, increased after they are exposed to oxygen limited conditions compared to fully anaerobic conditions. It was also observed that catechol 1,2‐dioxygenase enzyme activity increased by increasing the oxygen level on oxygen limited conditions in the anaerobic zone.     

腐泥型煤二次生烃热解模拟的荧光特征

通过对4块腐泥型煤原样及热解模拟残渣样的荧光特征的研究,对腐泥煤二次生烃特征加以探讨,得出主要结论为:(1)当开始二次生烃时,将出现蓝移现象,蓝移现象标志着二次生烃的开始;(2)蓝移现象发生的起始位置(镜质组反射率为标志)及蓝移现象的强度与样品的原始成熟度有密切关系,伴随着原始成熟度的提高,蓝移现象发生的成熟度位置将逐渐后移,其与初始成熟度的差值将先增大后减小,增大与减小的转折点推测位于1.4 6%~1.80%之间。      

文昌鱼胚胎整装荧光原位杂交技术的建立

文昌鱼(Branchiostoma belcheri tsingtauense)是进化发育生物学研究的重要模式生物,文昌鱼胚胎整装荧光原位杂交(WFISH,whole-mounted fluorescent in situ hybridization)技术将有助于鉴定文昌鱼胚胎发育过程中具有重要调控作用的功能基因。报告了文昌鱼胚胎整装荧光原位杂交技术,用以快速灵敏分析特定基因在文昌鱼胚胎发育过程中的时空表达图式。用体外转录合成的地高辛标记文昌鱼FGFR基因的反义RNA探针,检测到FGFR在文昌鱼原肠胚中表达于发生内陷的中内胚层细胞中,而预定发育为外胚层的细胞不表达FGFR。     

崇陵流域土石山区坡面优先流发育路径研究

大孔隙优先流对山坡产汇流过程有重要影响。为摸清太行山土石山坡不同坡位点(坡顶、坡中上、坡中、坡中下、坡脚)的大孔隙优先流发育路径规律,以崇陵流域的典型山坡为研究对象,采用亮蓝染色剂开展了野外双环入渗染色剂示踪试验,并从水平方向与垂直方向对比分析大孔隙优先流发育路径。结果表明:(1)从坡顶到坡脚,垂直方向优先流发育减弱,水平方向优先流增强。(2)坡中以下,大孔隙优先流水平发育明显;而坡中以上,垂直方向优先流发育明显,水平方向大孔隙优先流鲜有发育。(3)崇陵流域土山区坡面表层深度20 cm以上很少出现水平方向的优先流侧向补给,为垂直向下的活塞式下渗方式。20 cm以下开始出现水平方向的大孔隙优先流,30～70 cm为优先流发育显著区。以上结论可以为基于优先流的山坡产汇流模拟提供参考。