基于梯度依赖客观分析技术的全球Argo网格化数据集:构建及初步应用

采用梯度依赖相关尺度方法构建了1套2004—2017年间,月平均的全球海洋(0~1 500 m)1°×1°的Argo数据集,并在对该数据集进行对比检验的基础上,将其初步应用于中西太平洋黄鳍金枪鱼的渔场分析研究。结果表明,所构建的Argo数据集与WOA13数据集的温、盐偏差在上表层(150 m)稍大,最大值分别约为0.5 ℃和0.1,且偏差均随深度的增加而逐渐减小;其与TAO浮标时间序列的温度偏差,2004—2017年间均小于1 ℃,最大盐度偏差则小于0.5,且大部分海域接近0。中西太平洋海域,黄鳍金枪鱼中心渔场多集中在 28~29 ℃ 等温线范围内,在 22 ℃以下的海域单位捕捞努力量渔获量(catch per unit effort,CPUE)值极小;中心渔场区温跃层上界深度范围在20~120 m之间,且中心渔场在各个深度上形成的频数大体呈正态分布,温跃层上界深度为90 m时,形成中心渔场的可能性达到最大。研究表明所构建的数据集在水文环境分析及资源评估中有一定的应用价值。     

江苏海岸中部近岸冬季潮汐和潮流特征

南黄海西侧的江苏海岸近岸区域,素以地形复杂、潮流强劲、悬沙输运剧烈著称,但是较长期的同步潮位和潮流观测数据仍然缺乏,尤其是在近岸(20 km)浅水(20 m)区域。2014年1月在大丰港附近开展了连续潮位和潮流观测,获得的数据揭示了一系列特征。此地潮汐潮流为正规半日潮,浅水分潮显著。平均潮差为3.05 m,最显著的两个分潮为M2和S2分潮,振幅分别为1.45 m和0.52 m。潮流最显著的半日分潮M2分潮和最显著的浅水分潮M4分潮在沿岸方向上振幅分别为0.84m/s和0.12m/s,在跨岸方向上振幅分别为0.24 m/s和0.01 m/s,沿岸方向占绝对优势。潮波的沿岸传播介于前进波和驻波之间,驻波的特征稍强。M2分潮潮流椭圆最大流(长轴)方向为南偏东7.4°。存在冬季沿岸向北的余流,垂向平均值的大小为2.2 cm/s。以上潮汐潮流特征为该区域海洋物质输运研究提供了基础资料。     

地理信息系统在海岸海洋地貌研究中的应用

随着现代海洋观测技术的发展,通过海岸海洋地貌调查获得的数据急剧增长。运用地理信息系统(GIS)将不同学科、不同来源、不同格式的数据进行集成管理、分析和表达已成为当前海岸海洋地貌研究的重点。系统介绍了GIS技术在海岸海洋地貌研究中新的方法体系,包括数据获取、数据处理、数据分析和数据表达四个部分,重点研究了GIS空间技术、海底地形建模和用三维可视化技术解译海洋地学,探索陆海交互作用中隐含信息的方法,并将其应用到沿海城市规划、海港选址、大陆架岛礁地貌环境研究的具体实例中,研究范围从陆到海涵盖整个海陆交互作用带,为海岸海洋社会发展、经济建设及外交权益提供科学基础和决策支持。通过上述方法体系与应用研究说明,以GIS为核心建立的多源地理空间信息平台使传统海岸海洋地貌研究的范围、内容及方法都发生了重大变化,但多源数据信息的精确解译和成功应用必须同时具有空间信息学和地貌学专家知识。     

牙鲆GHR基因Promoter区微卫星序列多态性与生长性状关系的初步研究

采用牙鲆GHR基因5'端Promoter区的1个微卫星标记,对胶南和日照2个牙鲆养殖群体进行了群体遗传多样性的研究,并探索该基因多态性位点与牙鲆生长性状之间的相关性.结果表明,2个群体在该座位的等位基因数为12和9个,有效等位基因数为6.26和5.04个,多态信息含量为0.84和0.80.2个群体该座位的Hardy-Weinberg遗传偏离指数均为正值,并没有显示出杂合子缺失,但各基因型分布频率都在一定程度上偏离Hardy-Weinberg平衡(P<0.01).连锁分析中发现,在胶南群体中,IM基因型对应的个体在全重、全长、体长、头长、体高和眼径形态学数据中均是最大的;在日照群体中,BC基因型对应的个体在全重、全长、体高、尾柄高、尾柄长和眼径数据中均是最大的;而CJ基因型对应的个体在体长和头长这两组数据中是最大的.该结果为研究GHR基因的变异对鱼类生长发育的影响及探索将该基因作为生长遗传标记的可行性奠定了实验基础.     

波浪能发电平台系泊系统耦合动力响应及水动力分析

本文以一种新型波浪能发电平台为研究对象,分别采用基于三维势流理论的软件Sesam和基于有限体积法的Flow3D软件,对平台和发电浮子进行水动力分析。应用Sesam-HydroD模块计算了平台和发电浮子在频域内的运动响应,将平台和发电浮子的垂荡运动响应进行对比分析,结果表明,在正常海况下,平台与发电浮子垂荡运动相对幅值满足捕能系统的发电需求。应用Flow3D软件对平台整体进行水动力分析,结果表明,平台与发电浮子相对运动振幅在0.3～0.4m间,可满足发电需求。在此基础之上,应用Orcaflex软件,通过时域耦合动力分析的方法,计算了平台在自存工况和作业工况下的运动响应和系泊缆动张力响应,结果表明:在自存工况下,平台锚泊线的安全系数符合规范要求,平台具有良好的安全性能,能够适应恶劣的海洋环境;在作业工况下,平台的垂荡运动响应对波浪方向变化并不敏感,捕能系统不受波浪方向变化的影响,满足发电需求。另外,本文的研究结果能为类似的海洋平台的研究提供建议和参考。     

Comparative study of otolith and sulcus morphology for stock discrimination of yellow drum along the Chinese coast

Otolith morphology is widely used for fish stock identification. The sulcus, a structure on the medial side of the otolith, is an important feature in morphological analysis. This study was conducted to evaluate the feasibility of using sulcus morphology for stock identification and to compare its performance with commonly used otolith morphology analysis. Otoliths were collected and analyzed from three geographical groups (the Huanghe (Yellow) River estuary, HHE;the Jiaozhou Bay, JZB;and the Changjiang (Yangtze) River estuary, CJE) of yellow drum Nibea albiflora. The results show that the analysis of sulcus morphology based on shape indices (SIs), elliptic Fourier coefficients (EFc), and a combination of the two parameters identified stocks at overall classification rates of 51.0%, 72.5%, and 73.2%, respectively. These classification rates are similar to those obtained using otolith morphology analysis (57.0%, 73.8%, and 76.5% by SIs, EFc, and their combination, respectively). The findings suggest that sulcus morphology is comparable to the commonly used otolith morphology for identifying stocks of sciaenids, such as the yellow drum. For both otolith and sulcus morphology, EFc could identify the stocks more efficiently than SIs, while the combination of SIs and EFc was even better.     

Numerical Simulation on Flow Past Two Side-by-Side Inclined Circular Cylinders at Low Reynolds Number

A series of three-dimensional numerical simulations is carried out to investigate the effect of inclined angle on flow behavior behind two side-by-side inclined cylinders at low Reynolds number Re=100 and small spacing ratio T/D=1.5 (T is the center-to-center distance between two side-by-side cylinders, D is the diameter of cylinder). The instantaneous and time-averaged flow fields, force coefficients and Strouhal numbers are analyzed. Special attention is focused on the axial flow characteristics with variation of the inclined angle. The results show that the inclined angle has a significant effect on the gap flow behaviors behind two inclined cylinders. The vortex shedding behind two cylinders is suppressed with the increase of the inclined angle as well as the flip-flop gap flow. Moreover, the mean drag coefficient, root-mean-square lift coefficient and Strouhal numbers decrease monotonously with the increase of the inclined angle, which follows the independent principle at small inclined angles.     

An efficient hybrid integral-equation method for point-absorber wave energy converters with a vertical axis of symmetry

To assist in the prototyping and controller design of point-absorber wave energy converters (WECs), an easy-to-implement hybrid integral-equation method is presented for computing the frequency-domain hydrodynamic properties of bodies with a vertical axis of symmetry in waves. The current hybrid method decomposes the flow domain into two parts: an inner domain containing the body and an outer domain extending to infinity. The solution in the inner domain is computed using the boundary-element method, and the outer-domain solution is expressed using eigenfunctions. Proper matching at the domain boundary is achieved by enforcing continuity of velocity potential and its normal derivative. Body symmetry allows efficient computation using ring sources in the inner domain. The current method is successfully applied to three different body geometries including a vertical truncated floating cylinder, the McIver toroid, and the coaxial-cylinder WEC being developed in the authors’ laboratory. In particular, the current results indicate that, by replacing the flat bottom of the coaxial-cylinder WEC with the Berkeley-Wedge (BW) shape, viscous effect can be significantly reduced with only minor negative impact on wave-exciting force, thus increasing WEC efficiency. Finally, by comparing to experimental measurements, the current method is demonstrated to accurately predict the heave added mass and wave-exciting force on the coaxial-cylinder WEC with BW geometry. If a viscous damping correction factor is used, the heave motion amplitude can also be accurately computed.     

Coupled analysis for response and instability of sloping seabed under wave action

Wave-induced instability of seabed may cause damage to coastal and offshore structures. This issue has been investigated mostly for mildly sloping ($<5^{°}$) seabed considering uncoupled or one-way coupled response of wave and seabed interaction. However, some of the marine structures are founded on seabed with steeper slopes. In this study, the wave-induced response and instability of sloping seabed are evaluated using a coupled finite element model. The interaction between fluid and porous seabed accounting for the effect of fluid motion on the seabed response, and conversely the effect of seabed response on the fluid motion (but not on the surface wave profile) is considered. The results indicate that the system response (fluid pressure, stresses, etc.) and the extent of instantaneously liquefied zone within the sloping seabed with significant steepness are lesser than those for horizontal seabed. Moreover, for typical sediment and wave characteristics, for the flat seabed, the response obtained from fully coupled analysis is not significantly different from those obtained by uncoupled analysis. For the sloping bed, such difference is slightly greater as compared to that for the flat bed.     

文蛤亮氨酸氨肽酶LAP3的基因克隆及其在幼体不同发育时期、成体不同组织的表达分析

为探索贝类LAP3基因的结构和功能特征以及在生长发育过程中的作用,利用cDNA末端快速扩增（RACE）法获得文蛤LAP3（Mm-LAP3）基因的cDNA全长序列,并对其生物信息学、组织及发育时期表达特征进行了分析。结果显示,Mm-LAP3基因cDNA全长2 037 bp,ORF区1 254 bp,编码417个氨基酸;Mm-LAP3蛋白由Peptidase_M17超家族序列的N-端结构域和Peptidase_M17催化结构域组成。荧光实时定量PCR结果表明,Mm-LAP3基因在文蛤成体6个组织和幼体10个发育时期中均有表达,其中在斧足中的表达量显著高于其他组织（P<0.05）,这与斧足蛋白含量丰富、代谢旺盛相关;在幼体10个发育时期中的表达量呈现逐渐升高的趋势,在D形幼虫时期达到最高,随后又有所降低,推测Mm-LAP3基因在早期发育时期参与了某些组织器官的形成。