黄豆脲酶诱导碳酸钙沉淀多变量试验研究

植物源脲酶诱导碳酸钙沉淀（enzyme induced carbonate precipitation,简称EICP）可以显著改善砂土的工程力学特性,但在具体操作时,参数取值无对应规范,固化效果有待提升。基于黄豆脲酶,研究了温度、脲酶浓度、尿素浓度、钙浓度、pH值、钙源种类等变量对脲酶活性与碳酸钙沉淀的影响,并进行了沉淀物（碳酸钙晶体）的扫描式电子显微镜（scanning electron microscope,简称SEM）与X射线衍射（X-ray diffraction,简称XRD）测试,在此基础上开展了黄豆脲酶固化砂的无侧限抗压强度与固化效果试验研究。结果表明：脲酶活性随脲酶浓度的增加而线性增长,但存在温度阈值,温度超过阈值后,脲酶将完全失活,且阈值随脲酶浓度的增大而降低;尿素浓度与pH值共同影响脲酶活性,二者存在一个最优组合,当尿素浓度在0.1～1.0 mol/L时最优pH值为7,当尿素浓度在1.0～1.5 mol/L时最优pH值为8。脲酶是沉淀反应的催化剂,脲酶浓度越高,反应越完全,碳酸钙沉淀率越高;尿素与钙溶液则主要通过掺入量影响碳酸钙沉淀量,掺量比例宜为1:1,且二者浓度与pH值可通过影响脲酶活性来影响碳酸钙的沉淀情况;不同钙源对碳酸钙沉淀量的影响幅度不大。不同钙源沉淀碳酸钙晶体的成分与密度基本相同,但晶体结构差异较大,氯化钙沉淀碳酸钙晶体以块状为主,表面分布球状、类球状晶体,胶结面大,可作为EICP技术中较为理想的钙源。基于黄豆脲酶和氯化钙钙源固化砂的无侧限抗压强度约为掺粉煤灰砂样的6倍,通过SEM图像可发现,沉淀碳酸钙晶体包裹并黏结砂粒成为整体,固化效果非常理想。     

Experimental study of deformable connection consisting of buckling‐restrained brace and rubber bearings to connect floor system to lateral force resisting system

This paper presents experimental and numerical studies of a full‐scale deformable connection used to connect the floor system of the flexible gravity load resisting system to the stiff lateral force resisting system (LFRS) of an earthquake‐resistant building. The purpose of the deformable connection is to limit the earthquake‐induced horizontal inertia force transferred from the floor system to the LFRS and, thereby, to reduce the horizontal floor accelerations and the forces in the LFRS. The deformable connection that was studied consists of a buckling‐restrained brace (BRB) and steel‐reinforced laminated low‐damping rubber bearings (RB). The test results show that the force–deformation responses of the connection are stable, and the dynamic force responses are larger than the quasi‐static force responses. The BRB+RB force–deformation response depends mainly on the BRB response. A detailed discussion of the BRB experimental force–deformation response is presented. The experimental results show that the maximum plastic deformation range controls the isotropic hardening of the BRB. The hardened BRB force–deformation responses are used to calculate the overstrength adjustment factors. Details and limitations of a validated, accurate model for the connection force–deformation response are presented. Numerical simulation results for a 12‐story reinforced concrete wall building with deformable connections show the effects of including the RB in the deformable connection and the effect of modeling the BRB isotropic hardening on the building seismic response. Copyright © 2016 John Wiley & Sons, Ltd.     

A longer-term perspective on soil moisture,groundwater and stream flow response to the 2018 drought in an experimental catchment in the Scottish Highlands

The drought of summer 2018, which affected much of Northern Europe, resulted in low river flows, biodiversity loss and threats to water supplies. In some regions, like the Scottish Highlands, the summer drought followed two consecutive, anomalously dry, winter periods. Here, we examine how the drought, and its antecedent conditions, affected soil moisture, groundwater storage, and low flows in the Bruntland Burn; a sub-catchment of the Girnock Burn long-term observatory in the Scottish Cairngorm Mountains. Fifty years of rainfall-runoff observations and long-term modelling studies in the Girnock provided unique contextualisation of this extreme event in relation to more usual summer storage dynamics. Whilst summer precipitation in 2018 was only 63% of the long-term mean, soil moisture storage across much of the catchment were less than half of their summer average and seasonal groundwater levels were 0.5 m lower than normal. Hydrometric and isotopic observations showed that ~100 mm of river flows during the summer (May-Sept) were sustained almost entirely by groundwater drainage, representing ~30% of evapotranspiration that occurred over the same period. A key reason that the summer drought was so severe was because the preceding two winters were also dry and failed to adequately replenish catchment soil moisture and groundwater stores. As a result, the drought had the biggest catchment storage deficits for over a decade, and likely since 1975–1976. Despite this, recovery was rapid in autumn/winter 2018, with soil and groundwater stores returning to normal winter values, along with stream flows. The study emphasizes how long-term data from experimental sites are key to understanding the non-linear flux-storage interactions in catchments and the “memory effects” that govern the evolution of, and recovery from, droughts. This is invaluable both in terms of (a) giving insights into hydrological behaviours that will become more common water resource management problems in the future under climate change and (b) providing extreme data to challenge hydrological models.     

高含沙泥浆模型试验及其糙率纠正

国内许多年久失修的地下设施在废弃时面临巨大的安全隐患,而利用高含沙泥浆进行填充是有效而经济的方法。在进行填充前,对管道填充的可行性需要建立模型进行试验研究。在模型试验研究时遇到的模型不相似问题是影响模型试验准确性的重要因素,因此要对试验所得数据进行修正。在本次研究中我们通过对于试验所得流速数据进行糙率纠正解决了糙率不相似的问题。     

高温硬岩空气潜孔锤钻头设计

干热岩试验性开发井钻井施工,在高温硬岩的地层条件下,如何实现高效钻进仍存在很多技术难点。空气潜孔锤钻进技术是解决硬岩地层钻进的有效方法之一,潜孔锤钻头是空气潜孔锤钻进的关键器具。根据青海共和干热岩GH-01井空气潜孔锤钻进技术应用情况,分析探讨了空气潜孔锤钻头断齿、掉齿、磨损严重、使用寿命短等问题,从钻头结构设计、钻头材料选取、钻头冷压固齿工艺等几个方面对空气潜孔锤钻头进行了优化。固齿优化过程中采用有限元分析方法,对钻头冷压固齿过盈量进行仿真分析计算,根据分析计算结果确定最优固齿过盈量。通过对潜孔锤钻头结构、材料、加工等优化,为适用于高温硬岩的空气潜孔锤钻头研制提供了借鉴。为提高空气潜孔锤钻进综合效率,为干热岩钻探开发提供了技术支持和技术储备。     

酸改性红辉沸石特征研究

采用物理比表面积、扫描电镜、红外光谱、X射线衍射、差热等方法来表征酸改性后的红辉沸石矿石特征,获得通过酸改性后红辉沸石比表面积、孔容和原矿中的杂质都会发生变化,从而可提高红辉沸石的吸附和交换性能,达到改性红辉沸石处理污染物的能力。     

实验矿物学方法研究进展

基于3S技术的数字填图RGMAP区域地质调查技术实现了区域填图中数据采集的全过程信息化,其中背景图层数据参数的正确调整是数字化区调项目野外各项工作顺利开展的前提。本文根据笔者在数字填图技术应用中得切身体会,对背景图层参数不规范,比例尺不统一及路线或剖面导入后无法正常显示等常见问题的解决方法进行了较为详细的介绍。     

斥水性土壤水分入渗试验和模型

为研究斥水性土壤水分入渗规律,并探寻适用于斥水性土壤水分入渗的数学模型,以妫水河流域亲水性和斥水性土壤为研究对象,开展室内一维垂直土柱入渗试验;分别采用Kostiakov分段函数、Fourier级数、一阶Gaussian函数以及Gaussian分段函数对斥水性土壤入渗率进行拟合。试验结果表明:亲水性土壤入渗率随时间呈单调减小变化趋势,斥水性土壤入渗一段时间后累积入渗量会出现翘尾现象,入渗率为先增大后减小的单峰曲线。模型图形分析表明: Kostiakov分段函数入渗率在拐点处同时存在一个极大值和极小值,Fourier级数存在多个波峰,一阶Gaussian函数不能反映入渗率在拐点后大于拐点前的试验现象,因而均难以真实反映斥水性水分入渗过程。忽略水分开始快速入渗过程,Gaussian分段函数模型不仅能够反映入渗率在拐点前的单调增以及拐点后的单调减过程,同时也能够体现入渗率在拐点后大于拐点前的试验现象。     

同轴双向回转钻进破岩效率研究

国内外相关研究均显示同轴双向回转可有效降低钻具振动和提高破岩效率,但双向回转相对传统单向回转的钻进效率的提升水平研究较少。据此,本文建立双向回转钻头以及单向回转钻头与岩石相互作用的有限元模型,以破岩比功作为评价指标量化比较分析了2种钻头的破岩效率。通过定义双向钻头内外壁尺寸差、内外钻头的间隙、内钻头壁厚与双向钻头内外壁尺寸差之比、内外钻头转速比和内外钻头底部高度差5种核心参数,并设计正交试验完成了仿真分析。结果显示,内外钻头高度差与内外钻头间隙对于破岩比功影响比较小,内外钻头尺寸差、内外钻头转速比和内钻头壁厚与双向钻头内外尺寸差之比对破岩比功影响显著。     

菲律宾蛤仔碱性磷酸酶的分离纯化及部分性质研究

经Tris-HCl缓冲液浸提、硫酸铵分级沉淀盐析、DEAE-Sepharose Fast Flow离子交换层析和Sephadex G-75凝胶过滤层析等步骤,从菲律宾蛤仔内脏团中分离得到碱性磷酸酶,SDS-PAGE显示为单一条带,提纯倍数为14.42,比活力达到131.08U/mg。酶学性质研究表明:该酶亚基分子量约44kD,最适反应温度为45℃,最适pH值为9.0,米氏常数Km为0.098mmol/L,最大反应速度Vmax为1.01μmol/(mL·min)。Ca2+、Mg2+对酶活力表现出显著的激活作用。