大银鱼胚胎发育的观察

根据1975、1979和1982三年冬季在洪泽湖、淮河和太湖人工授精以及采自产卵场的发育受精卵,于实验室内孵化、培育所得的材料,对大银鱼的卵子和前期仔鱼的发育特征作了描述。     

温度和盐度对方斑东风螺胚胎发育的影响

研究了温度和盐度对方斑东风螺胚胎发育的影响。结果表明:方斑东风螺胚胎发育的最低和最高临温分别为20℃和32℃,最适温度范围为24~28℃,在此温度范围内,方斑东风螺胚胎发育所需时间[T(h) ]温[X(℃) ]呈负相关关系,它们之间的回归关系符合指数函数方程:T=438 .30e-0. 043 3X(R2 =0. 982 3,P<0 胚胎发育的最低和最高临界盐度分别为14和32,最适盐度范围为20 ~28,在适宜盐度范围内,不同盐度对方风螺的胚胎发育速度无显著影响。     

暗纹东方鲀的人工繁殖

１９９５－１９９７年,以暗纹东方Ｔｕｎ进行人工繁殖,经ＬＨＲＨ－Ａ２催熟处理,雌鱼的性腺可以发育到Ⅳ期末,性腺发育达到Ⅳ期中以后的雌鱼注射激素能引进正常的排卵反应,试验用的催产药物为ＬＨＲＨ－Ａ２,ＨＣＧ和ＰＧ。在水温１６－２１℃的条件下,效应时间为６－８６ｈ,平均催产率为８７．５％,平均受精率为６１．８％,平均孵化率为５１．７％。     

Three‐dimensional Evolutionary Models of the Qiongxi Structures,Southwestern Sichuan Basin,China: Evidence from Seismic Interpretation and Geomorphology

Fold terminations are key features in the study of compressional fault-related folds. Such terminations could be due to loss of displacement on the thrust fault or/and forming a lateral or oblique ramp. Thus, high-quality seismic data would help unambiguously define which mechanism should be responsible for the termination of a given fault-related fold. The Qiongxi and Qiongxinan structures in the Sichuan Basin, China are examples of natural fault-propagation folds that possess a northern termination and a structural saddle between them. The folds/fault geometry and along-strike displacement variations are constrained by the industry 3-D seismic volume. We interpret that the plunge of the fold near the northern termination and the structural saddle are due to the loss of displacement along strike. The fault geometry associated with the northern termination changes from a flat-ramp at the crest of the Qiongxinan structure, where displacement is the greatest, to simply a ramp near the northern tip of the Qiongxi structure, without forming a lateral or oblique ramp. In this study, we also use the drainage pattern, embryonic structure preserved in the crest of the Qiongxinan structure and the assumption that displacement along a fault is proportional to the duration of thrusting to propose a model for the lateral propagation of the Qiongxinan and Qiongxi structures. Specifically, we suggest that the structure first initiated as an isolated fault ramp within brittle units. With increased shortening, the fault grows to link with lower detachments in weaker shale units to create a hybridized fault-propagation fold. Our model suggests a possible explanation for the lateral propagation history of the Qiongxinan and Qiongxi structures, and also provides an alternative approach to confirming the activity of the previous Pingluoba structure in the southwestern Sichuan Basin in the late Cenozoic.     

犬齿牙鲆胚胎及仔、稚、幼鱼形态发育观察

犬齿牙鲆(Paralichthys dentatus)卵为端黄卵,受精卵浮性,卵径950~1100μm,油球径200~225μm.在水温20.5~22.0℃,盐度33条件下培育,受精后1 h 20 min 进入卵裂期,6 h 10 min 进入囊胚期,11 h 20 min 进入原肠胚期,19 h 10 min 发育到胚孔封闭期,胚体形成,26 h 50 min 尾芽出现,33 h 35 min心跳开始,48 h 10 min 开始孵化出仔鱼.初孵仔鱼平均全长2.59 mm±0.02 mm,在培育水温为17.0~21.0℃,盐度24~34条件下,胚后发育时序为:1日龄~9日龄为仔鱼前期,10日龄平均全长5.52 mm±0.05 mm,卵黄囊和油球完全吸收进入仔鱼后期;40日龄平均全长20.82 mm±0.67 mm,各鳍鳍条发育形成进入稚鱼期;65日龄平均全长41.13 mm±0.88 mm,全身鳞被完整进入幼鱼早期     

水温和余氯对泥蚶胚胎发育的毒性效应

为了研究三门湾海域温排水污染对水生动物发育的影响,本实验通过室内模拟三门湾核电站温排水的余氯残留和温升效应,研究了不同温度和不同游离余氯质量浓度耦合下对泥蚶(Tegillarca granosa)胚胎发育的影响.实验水温为29℃、32℃和35℃,游离余氯质量浓度为0.025、0.050、0.100、0.200、0.40...     

星斑裸颊鲷胚胎及卵黄囊期仔鱼发育研究

对星斑裸颊鲷(Lethrinus nebulosusForska.l)的胚胎发育过程做了详细的观察和描述,并跟踪了孵化仔鱼的生长情况。结果表明,星斑裸颊鲷受精卵为浮性卵,卵径0.761 mm±0.019 mm,在水温27℃±1℃,盐度30~32的条件下,受精后约16.5 h孵化;在水温25℃±1℃,盐度30~32的条件下,受精后约17.83 h孵化。初孵仔鱼全长1.739~1.772 mm,仔鱼孵出2 d后开口,卵黄囊与油球已基本消耗完毕,大部分仔鱼开始摄食L-S型轮虫,开始由内源营养期向外源营养期过渡。同时发现在不同温度下,胚胎发育的时间、初孵仔鱼的大小、生长速度及卵黄囊的大小均不同。     

黄条鰤(Seriola aureovittata)MyHC基因克隆及其在早期发育阶段表达研究

为解析肌球蛋白重链(MyHC)基因在黄条鰤(Seriola aureovittata)生长发育过程中作用机制,本研究采用RACE技术,克隆了黄条鰤MyHC基因全长cDNA序列,采用荧光定量PCR(qRT-PCR)方法对MyHC在黄条鰤不同组织、胚胎发育过程以及仔稚幼鱼发育过程中的表达特征进行了研究。结果表明:黄条鰤MyHC基因全长为6143bp,开放阅读框为5811bp,编码了1936个氨基酸,由3个保守结构域组成即MYSc-classⅡ、Myosin taill和SH3;系统进化树分析显示黄条鰤MyHC与高体鰤进化关系最近。qRT-PCR分析发现黄条鰤MyHC在各组织中均有表达,但在肌肉中表达量最高(P<0.05);随着黄条鰤胚胎发育的进行,MyHC在16细胞期之前表达量较高,在胚体下包2/3时期表达量显著升高,且在孵化期达到峰值(P<0.05:);在仔稚幼鱼发育阶段,MyHC在孵化后20d后表达量显著升高,30d表达水平达到峰值(P<0.05),随后的35d到40d表达水平略有下降但仍保持较高表达趋势,黄条鰤MyHC表达具有发育阶段表达的特异性。MyHC表达特征揭...     

菊黄东方纯胚胎及仔稚幼鱼的发育

本文报道了菊黄东方纯胚胎及仔稚幼鱼发育情况．其结果表明：在水温20～21℃、盐度20～30、pH值8．0～8．3的条件下，受精卵受精后2h为2细胞期、15h为囊胚期、30h为原肠期、55h为胚体期、152h孵出仔鱼；孵出仔鱼在水温19～28℃、盐度20～30、pH8．0～8．3条件下，孵化后5d开口、18d进入稚鱼期、40d进入幼鱼期．此外，对胚胎及仔稚幼鱼各发育阶段进行较详细描述、测量并给出示意图．