6-磷酸山梨醇脱氢酶(gutD)基因克隆、测序和表达

本研究根据6-磷酸山梨醇脱氢酶基因(gutD)两端序列设计引物，以假单胞杆菌(Pseudonmonas XM)的总DNA为模板通过PCR的方法克隆gutD基因并进行序列分析，将克隆得到的gutD基因与原核表达载体pBV220连接并转化大肠杆菌(Escherichia coli)JM101后检测其蛋白表达及菌株生长耐盐性。     

Fischer图解及其在旋回层序研究中的应用──以北京西山张夏组为例

Ｆｉｓｃｈｅｒ图解（又称为容纳空间图解）为人们研究沉积旋回在空间上的叠置规律、相对海平面变化、层序级次的划分以及地层层序对比等提供了一种客观实用的方法。本文较详细地讨论了该图解的绘制方法及应注意的问题。运用该图解，将北京西山下苇甸剖面张夏组划分为１个大的三级旋回层序和４个四级旋回层序，根据图解反映的相对海平面变化讨论了旋回层序与构造运动间的关系，提出华北板块晚寒武世之前的＂翘翘板运动＂应始于中寒武世张夏期之早期到中期。     

Molecular ecological study of <Emphasis Type="Italic">Siganus spinus</Emphasis> and <Emphasis Type="Italic">S. guttatus</Emphasis> from Okinawan waters based on mitochondrial DNA control region sequences

The mitochondrial DNA (mtDNA) control region was sequenced to examine the genetic variability and gene flow of juvenile Siganus spinus and S. guttatus. In total, 461 nucleotide sequences were obtained from 69 specimens of juvenile S. spinus from Okinawa Island and Ishigaki Island, in which 17 variable sites and 22 haplotypes were identified. Haplotype diversity (h) was high (0.9244 in Okinawa and 0.8984 in Ishigaki), whereas nucleotide diversity (π) was low (0.0063 in Okinawa and 0.0059 in Ishigaki). The two populations were not genetically distinct. Siganus guttatus, which do not form large schools at recruitment, in contrast S. spinus, were also analyzed by studying 152 individuals collected off Okinawa Island, Miyako Island, and Ishigaki Island. Of 476 nucleotide sequences, 50 were variable, and 42 haplotypes were identified. Genetic variability values were high (h = 0.8766 and π = 0.0151 in Okinawa; h = 0.9640 and π = 0.0192 in Miyako; h = 0.9161 and π = 0.0199 in Ishigaki). The Okinawa population was genetically isolated from the Miyako and Ishigaki populations. As a result, genetic diversity was high for each of these siganid populations despite their being target species for fisheries; however, the degree of inter-population gene flow was higher for S. spinus than S. guttatus, suggesting that these species exhibit different dispersal strategies.     

Aequorea taiwanensis n. sp. (Hydrozoa, Leptomedusae) and mtCOI sequence analysis for the genus Aequorea

Aequorea taiwanensis, a new hydrozoan species from the Taiwan Strait was described using morphological and molecular characteristics. Both morphological and mitochondrial cytochrome oxidase subunit I (mtCOI) data supported A. taiwanensis n. sp. as a valid species. Sequence divergence and genetic distance of A. taiwanensis n. sp., A. papillata and A. conica were analysed based on the mtCOI gene sequences. The mtCOI sequences from these three species of the genus Aequorea showed high variation frequency, with sequence divergences ranging from 9.10% to 11.9%, and pairwise genetic distances ranging from 0.097 to 0.130. MtCOI sequence analysis provided diagnostic molecular systematic characteristics for accurate identification and discrimination of the species of Aequorea or their populations, and will be used to resolve evolutionary relationships among them.It was suggested that 10%—20% pairwise mtCOI sequence differences indicated the specieslevel divergence among congeneric species in the Hydromedusae.     

Mineralogical characteristics of polymetallic sulfides from the Deyin-1 hydrothermal field near 15°S,southern Mid-Atlantic Ridge

A seafloor hydrothermal field, named Deyin-1 later, near 15°S southern Mid-Atlantic Ridge(SMAR) was newly found during the 22 nd cruise carried out by the China Ocean Mineral Resources Research Development Association(COMRA). Sulfide samples were collected at three stations from the hydrothermal field during the26 th cruise in 2012. In this paper, mineralogical characteristics of the sulfides were analyzed with optical microscope, X-ray diffractometer, scanning electron microscope and electron microprobe to study the crystallization sequence of minerals and the process of hydrothermal mineralization. According to the difference of the ore-forming metal elements, the sulfide samples can be divided into three types:(1) the Ferich sulfide, which contains mainly pyrite and chalcopyrite;(2) the Fe-Cu-rich sulfide consisting predominantly of pyrite, chalcopyrite and isocubanite, with lesser amount of sphalerite, marmatite and pyrrhotine; and(3) the Fe-Zn-rich sulfide dominated by pyrite, sphalerite and marmatite, with variable amounts of chalcopyrite, isocubanite, pyrrhotine, marcasite, galena and gratonite. Mineral precipitations in these sulfides are in the sequence of chalcopyrite(isocubanite and possible coarse pyrite), fine pyrite,sphalerite(marmatite), galena, gratonite and then the minerals out of the dissolution. Two morphologically distinct generations(Py-I and Py-II) of pyrite are identified in each of the samples; inclusions of marmatite tend to exist in the coarse pyrite crystals(Py-I). Sphalerite in the Fe-Zn-rich sulfide is characterized by a"chalcopyrite disease" phenomenon. Mineral paragenetic relationships and a wide range of chemical compositions suggest that the environment of hydrothermal mineralization was largely changing. By comparison, the Fe-rich sulfide was formed in a relatively stable environment with a high temperature, but the conditions for the formation of the Fe-Cu-rich sulfide were variable. The Fe-Zn-rich sulfide was precipitated during the hydrothermal venting at relatively low temperature.     

层序成因动力学中的构造因素研究

本文以层序－盆地－构造为主线，对层序成因动力学中的构造控制进行了研究。结果表明，层序发育的构造控制主要表现为：1）构造控制盆地边界，进而控制层序充填轮廓；2）构造级次控制层序和层序级别；3)构造属性控制盆地性质，进而控制层序成因格架和界面成因类型；4)随着层序规模和界面级别的增大，构造控制的意义和痕迹越明显；5)同沉积断裂活动控制层序的内部构型；6)构造基底活动形式控制着层序容纳空间和结构型式；7)构造演化控制着盆地演化及性质，进而控制着层序充填样式和组合类型。     

三肇凹陷葡萄花油层高分辨率层序地层划分及沉积特征研究

应用高分辨率层序地层学理论,通过对各级基准面旋回的沉积动力学分析,依据１０口井岩心和１２５６口井测井资料,把松辽盆地三肇凹陷葡萄花油层划分为１个长期基准面旋回、２个中期基准面旋回、１２个短期基准面旋回,提出了葡萄花油层南部为同沉积构造抬升控制的中部缺失而非底部缺失的层序地层新格架。通过对短期基准面旋回内储层沉积特征、单砂体展布规律的研究,认为葡萄花油层主要储层水下河道砂体比原认识更连续,且延伸较远, 分流平原、内前缘相带南移达３６ｋｍ,这对深入认识该区油藏类型、聚油规律、特别是高含水期剩余油挖潜具有重要意义,对指导整个松辽盆地北部岩性油气藏勘探与开发具有重大影响。     

Microbial release of potassium from K-bearing minerals by thermophilic fungus Aspergillus fumigatus

A strain of thermophilic fungus Aspergillus fumigatus was cultured with K-bearing minerals to determine if microbe-mineral interactions enhance the release of mineralic potassium. Experiments were carried out in two settings, one with the mineral grains and the fungal cells in direct contact, and the other employing a membrane (pore size 0.22 μm) to separate the two. Measurements over a period of 30 days showed that, irrespective of the experimental setup, the concentration of free K in the culture was drastically higher than those in any of the control experiments where no living organism was present. Moreover, the occurrence of mineral-cell physical contact enhanced potassium release by an additional factor of 3 to 4 in comparison to the separation experiments. For contact experiments, Electron Probe Microanalysis revealed the formation of mycelium-mineral aggregates, and Atomic Force Microscopy imaging further indicated the possible ingestion of mineral particles by the fungus cells. Contrasting to what was observed and expected in control experiments, the potassium solubilization rate showed a positive dependence upon pH when fungi and minerals were mixed directly, and exhibited no correlations with solution acidity if cell-rock contact was restrained. These results appear to suggest that A. fumigatus promoted potassium release by means of at least three likely routes, one through the complexation of soluble organic ligands, another appealing to the immobile biopolymers such as the insoluble components of secretion, and the third related to the mechanical forces in association with the direct physical contact between cells and mineral particles.     

惠民凹陷唐庄地区沙河街组层序地层和沉积相

针对研究区的区域地质概况,并借鉴了前人的研究成果,利用地震和测井资料识别出了三级层序边界,并对各层序进行了体系域划分,建立了研究区的等时地层格架。在此基础上,通过对岩芯和测井相的综合分析,以体系域为单位进行了沉积相的划分,建立了研究区的精细沉积相模型,并对沉积相的展布特征进行了分析。     

祁连-海原地震带中强地震序列类型分布及其应用

研究了1970年以来发生于祁连—海原地震带的18次中强地震序列特征，在此基础上确定了地震序列类型，研究了各种类型地震序列的分布规律．结果表明，该带的18次中强地震序列中有44%为主震型，56%为孤立型．西海固地区的中强地震序列一般为孤立型；101°～103.5°E范围内的祁连山中东段的中强地震序列一般为主震型；99°～101°E范围内的祁连山中段为孤立型和主震型序列并存；99°E以西的祁连山西段的中强地震序列为孤立型．将上述结果应用于2000年6月6日景泰MS5.9地震的震后趋势快速判定，判定结果与实际地震活动情况符合．