用钨酸钠分离法研究诏安湾甲藻休眠孢子

本文介绍了一种分离富集沉积物中甲藻休眠孢子的新方法－钨酸钠法，并用这种方法在诏安湾沉积物中分离到了12种甲藻休眠孢子。     

Use of Cell-Specific PAM-Fluorometry to Characterize Host Shading in the Epiphytic Dinoflagellate Gambierdiscus toxicus

Abstract. Cell‐specific fluorescence characteristics were used to characterize the light tolerance of the toxic benthic dinoflagellate Gambierdiscus toxicus. The fluorescence parameter F_v : F_m was measured using pulse amplitude modulation (PAM) fluorometry on individual cells collected from foliose red algae growing in the sub‐tidal margin of South Water Cay, Belize. Samples were collected over several days during sunny and cloudy conditions and compared to samples incubated in situ. The data from individual cells were used to generate both F_v : F_m frequency histograms and averages. Maximum individual cell values of F_v : F_m reached 0.81 in pre‐dawn samples, a value near the theoretical maximum for PAM fluorometry. In field samples from macroalgal hosts, average F_v : F_m values declined only slightly during the day, but cells incubated in bottles under 47 % incident sunlight showed a significant mid‐day depression. In freshly collected samples, near‐maximum F_v : F_m values could be found in individual cells during the entire day; however, the frequency histograms indicated a greater range in F_v : F_m values during the afternoon than in the morning. In contrast, cultures of G. toxicus showed a tight distribution around a mean. Field samples showed a rapid recovery to near‐maximum F_v : F_m within 2 min when assayed using a standardized actinic light series. Similar results were obtained in laboratory cultures of G. toxicus grown at 73 µmol photons · m^‐2 · s^‐1, but not at 383 µmol photons · m^‐2 · s^‐1. These data provide empirical support for suggestions that G. toxicus exploits the three‐dimensional structure of the algal host thallus to minimize light exposure. This strategy permits G. toxicus, a high‐light intolerant species in culture, to thrive in shallow, well‐lit tropical seas. It may also partially explain the observed preference of G. toxicus for complex, foliose macroalgae as hosts.     

甲藻赤潮与水体中营养盐的关系初探

作者在国家海洋局第三海洋研究所的陆基水池中，应用围隔实验技术，排除了水动力、盐度等环境因子的变动性的干扰，人工诱发甲藻赤潮．在这个围隔实验的两个围隔体中，分别发生了一种裸甲藻（Gymnodinium sp.）和海洋原甲藻（Prorocentum micans）的赤潮．在赤潮发生和发展过程中所监测到的营养盐数据表明有些甲藻增殖不一定需要水体中高营养盐；营养盐供给的多寡会导致甲藻赤潮优势种的差异和赤潮规模．     

The Early and Middle Miocene transgression at the southern border of the North Sea Basin (northern Belgium)

The Lower–Middle Miocene Berchem Formation of northern Belgium is an essentially sandy sequence with a varying glauconite content and often abundant shelly intervals. The formation was deposited in a shallow marine environment and rests unconformably on stiff Rupelian clays or Chattian sands. The lithological recognition of the four members (Edegem Sands, Kiel Sands, Antwerpen Sands and Zonderschot Sands members) of the Berchem Formation solely based on lithological criteria proved to be difficult, especially in boreholes. The geometry of the Formation in the subsurface of northern Belgium remained largely unknown. Diverse and well preserved dinoflagellate cyst associations have been recovered from the four members in seven boreholes and two outcrops, and allow a refinement of the biostratigraphy of these deposits. A Miocene biozonation defined in mid‐latitude shallow marine deposits in the Atlantic Coastal Plain of the USA (Salisbury Embayment, Maryland) is readily applicable to this material, and has led to a detailed stratigraphic assessment of each member. Three detailed profiles depicting the distribution of the biozones in the subsurface of northern Belgium allow the reconstruction of the geometry and depositional history of the Berchem Formation. The oldest Miocene deposits are of early Burdigalian age and they testify to a transgression, which invaded Belgium from a north–northwestern direction. The maximum flooding took place during early Serravallian times. The upper boundary of the formation is a major erosional surface of late Serravallian or (slightly) younger age. Copyright © 2005 John Wiley & Sons, Ltd.     

1998年春季珠江口海域大规模赤潮原因生物的形态分类和生物学特征Ⅱ

对 １９９８年 ４月 １５日采集于广东珠江口万山群岛桂山岛网箱养殖区的赤潮新鲜水样和福尔马林固定样品进行赤潮原因生物的形态分类和生物学特征的研究。结果发现,此次赤潮共有 ７种原因生物,全部属于裸甲藻类,其中最占优种三宅裸甲藻（Ｇｙｍｎｏｄｉｎｉｕｍ　 ｍｉｋｉｍｏｔｏｉ）和优势种斯氏多沟藻（Ｐｏｌｙｋｒｉｋｏｓ ｓｃｈｗａｒｔｚｉｉ）、螺旋螺沟藻（Ｇｙｒｏｄｉｎｉｕｍ ｓｐｉｒａｌｅ）的形态分类及生物学特征研究结果已在本系列论文的第一篇报道。本文报道其余 ４种红色裸甲藻（Ｇｙｍ．ｓａｎｇｕｉｎｅｕｍ）、灰白下沟藻（Ｋａｔｏｄｉｎｉｕｍ ｇｌａｕｃｕｍ）、螺沟藻９８型（Ｇｙｒｏｄｉｎｉｕｍ ｓｐ． ｔｙｐｅ－９８）和多环旋沟藻（Ｃｏｃｈｌｏｄｉｎｉｕｍ　 ｐｏｌｙｋｒｉｋｏｉｄｅｓ）的形态分类和生物学特征。其中,红色裸甲藻是此次赤潮的优势种之一,灰白下沟藻和多环旋沟藻在我国是新记录,螺沟藻９８型则可能是新种。     

台湾海峡浮游甲藻的分布特征和变化趋势

根据2006-2007年“国家908”专项台湾海峡4个航次调查,分析该海域浮游甲藻的种类组成、群落结构和时空分布特征。共鉴定甲藻18属131种,其中高温高盐种为主体,占总种数的72．52％,其次为广温广盐种,占总种数的25．19％,近岸种仪占2．29％。夏季甲藻的种类最丰富,而春季甲藻的丰度最高。平均丰度为404．96×10^2cells／m3,其平面分布呈现从近岸向外海、从北向南递增的趋势。与1984-1985年的调查结果相比较,甲藻丰度的平面分布格局和季节变化趋势没有明显变化,但丰度增加3．01倍。从季节变化看,冷季甲藻丰度增加较为显著;从平面分布看,台湾海峡北部增加较多。此外,该海域甲藻群落结构也发生变化,种类多样性指数和均匀度上升,其丰度占浮游植物总丰度的比例由0．55％上升到1．02％。     

长江口海域春季浮游植物的年际变化

于2014年5月在长江口海域采集海水样品,分析了浮游植物种类组成、细胞丰度、优势种等群落结构特征。共鉴定浮游植物105种,其中硅藻30属56种,甲藻19属41种,以及隶属于金藻、蓝藻等的8个种。浮游硅藻以近岸半咸水种为主,优势种类是中肋骨条藻(Skeletonema costatum);浮游甲藻以沿岸广布种为主,典型代表是东海原甲藻(Prorocentrum donghaiense)。为了更好地了解长江口海域浮游植物的年际变化趋势,本文收集整理了该海域1986—2014年间的春季数据,分析了浮游硅藻、浮游甲藻主要群落特征的年际变化,以及浮游硅藻优势种类细胞粒径的变化趋势。结果表明:(1)浮游硅藻、浮游甲藻的种类数均呈现不同程度的上升趋势,浮游甲藻的上升幅度更为明显;(2)浮游甲藻种类数在浮游植物种类数中的占有比例呈上升趋势,而浮游硅藻所占比例呈下降趋势;(3)浮游硅藻优势种类的细胞粒径呈现小型化趋势。     

胶州湾表层沉积物中甲藻孢囊的分布

为了解胶州湾表层沉积物中甲藻孢囊的分布特征,于2015年7月采集胶州湾12个站位的表层沉积物进行甲藻孢囊分析,共鉴定甲藻孢囊37种(分属5个类群)和2种未定种,包括自养型20种和异养型17种,优势种为膝沟藻。其Shannon-Wiener多样性指数[H′(log2)]介于2.84—3.91,平均值为3.42;均匀度指数介于0.75—0.98,平均值为0.88,总体值较高。胶州湾海域各站位的甲藻孢囊丰度介于96—969cyst/g DW(干重),平均丰度为401cyst/g DW,呈现出临近湾口处丰度较高,而湾内较低的空间分布差异特征。尤其值得注意的是,有两种产虾夷扇贝毒素(Yessotoxin,YTX)甲藻[具刺膝沟藻(Gonyaulax spinifera)和多边舌甲藻(Lingulodinium polyedrum)]的孢囊和3种产麻痹性贝类毒素(PSP)甲藻[塔玛/链状亚历山大藻(Alexandrium catenella/tamarense)、微小/相似亚历山大藻(A.minutum/affine)和链状裸甲藻(Gymnodinium catenatum)]的孢囊在胶州湾海域大多数站位均有分布,尽管其数量不高,但其广泛的分布范围仍需引起我们的重视。     

非海相白垩纪沟鞭藻生物地层学

现代沟鞭藻可以在淡水中生存,在1995年美国报道了中新世淡水多甲藻类沟鞭藻后,证实了有淡水沟鞭藻化石的存在。白垩纪非海相沟鞭藻最早发现于英格兰南部,之后在澳大利亚西南端以及中国的很多陆相沉积盆地中陆续被报道。本文在了解白垩纪非海相沟鞭藻的组合面貌和它们的地质时代以及地层对比关系的基础上,讨论淡水沟鞭藻的生物地层和古环境意义。中国白垩纪沟鞭藻组合面貌如下:早白垩世时松辽盆地的非海相沟鞭藻组合自下而上为:沙河子组和营城组的Vesperopsis-Balmula组合、登楼库组的Vesperopsis(Vesperopsis)zhaodongensis组合、泉头组三、四段的Quantouendinium dictyophorum-Operculodinium组合、青山口组的Kiokansium-Tetrachacysta tuberculata组合、姚家组二、三段的Dinogymniopsis daqingensis组合、嫩江组一、二段的Cleistosphaeridium-Dinogymniopsis minor组合、嫩江组三、四段的Nenjiangella granulata-Valensiella dictyophora组合、四方台组中的Pediastrum-Aquadulcum组合和明水组中的Tetranguladinium组合。黑龙江省三江盆地的非海相沟鞭藻组合自下而上为:城子河组的Nyktericysta(Nyktericysta)puyangensis-Vesperopsis(Vesperopsis)sanjiangensis组合、Oligosphaeridium-Nyktericysta-Vesperopsis组合、Vesperopsis(Vesperopsis)sanjiangensis组合、Nyktericysta(Nyktericysta)puyangensis-Lecaniella proteiformis组合和Vesperopsi,(Ves-peropsis)jixianensis-Vesperopsis(Vesperopsis)suibinensis组合、穆棱组的Quantouendinium cf.dictyophorum组合。黑龙江省鸡西盆地滴道组有Vesperopsis(Vesperopsis)didaoensis-Lagenorhytis granorugosa非海相沟鞭藻组合。吉林省延吉盆地有铜佛寺组二段的Vesperopsis(Vesperopsis)cf.zhaodongensis-Vesperopsis(Vesperopsis)yanjiensis非海相沟鞭藻组合和铜佛寺组三段的Filisphaeridium-Sentusidinium非海相沟鞭藻组合;大砬子组中有零星非海相沟鞭藻出现,未能建组合。辽宁省阜新盆地阜新组产Australisphaera fragilis-Ripea sussexensis非海相沟鞭藻组合。内蒙古开鲁盆地非海相沟鞭藻共自下而上分为5个组合:九佛堂组沙海组Quantouendinium cf.microreticulata-Vesperopsis(Vesperopsis)suibinensis组合、阜新组Vesperosis(V.)sp.2-Quantouendinium cf.dictyophorum组合、姚家组Concentricystes组合、嫩江组Leiosphaeridia组合、四方台组Subtilisphaera-Pediastrum组合。内蒙古海拉尔盆地非海相沟鞭藻自下而上可分为5个组合:南屯组Protoellisodinium fibratum-Dinogymniopsis daqingensis组合、大磨拐河组一段上部至二段Versperopsis(Contrangularia)granulata-Vesperopsis(Contrangularia)reticulata组合、大磨拐河组三段Nyktericysta(Nyktericysta)reticulata组合、大磨拐河组四段Palaeoperidinium-Pareodinia组合和伊敏组一段Nyktericysta(Hailaera)hailaerensis组合。内蒙古二连盆地非海相沟鞭藻自下而上2个组合:赛汗塔拉组三段Parabohaidina-Fromea-Quantouendinium-Vesperopsis组合和哈达图组Porusphaeraerenensis组合。江苏省淮安地区泰州组有Palaeoperidinium-Laciniadinium非海相沟鞭藻组合。晚白垩世时广东省三水盆地大塱山组含两个非海相沟鞭藻组合(自下而上):Palaeostomocystis-Campenia组合和Saeptodinium-Laciniadinium组合;湖北省江汉盆地渔洋组有Saeptodinium-Laciniadinium非海相沟鞭藻组合;海南省乐东地区鹿母湾组有Laciniadinium granulatum-Granodiscusgranulatus非海相沟鞭藻组合。国外已报道的非海相沟鞭藻有英格兰Wessex-Weald盆地Weald Clay群Wessex组和Vectis组的Australisphaerafragilis-Ripea sussexensis组合;澳大利亚西南部Perth盆地Parmelia组的Fusiformacystatumida组合带。这些组合中所含沟鞭藻大多为地方性属种,能进行洲际地层对比的属种较少,因此它们的地质时代的确定有时还要依靠其他生物门类和手段。非海相沟鞭藻组合有一些共同的特征,如分异度通常都较低,但丰度可变;沟鞭藻囊孢的形态与海相的相比形态和纹饰都较简单等。一些非海相的属如Nyktericysta和Vesperopsis可视作早白垩世的标准化石,但白垩纪非海相沟鞭藻组合中的多数种都是地方性的,这些组合之间很难直接对比,也不能与海相组合作种级的对比,因此确定它们的地质时代经常需要依靠其他化石。非海相沟鞭藻组合中不同组成分子可以指示古盐度的变化,如角藻类囊孢占主导时,表示接近于淡水,而膝沟藻类囊孢占主导时,则为微咸水或半咸水。古盐度的变化可能是盆地附近海平面变化和古气候变化的结果。一些淡水和微咸水的其他藻类与沟鞭藻的共同存在,如Pediastrum,Scenedesmus和Botryococcus等,是指示古环境的证据之一。虽然非海相沟鞭藻的发现颠覆了传统认为沟鞭藻是海相的观念,但是并不意味着它们不能指示古环境,因为非海相与海相沟鞭藻组合是可以区分开来的。     

塔里木盆地北部塔河油田原油分子地球化学特征及成因类型划分

GC、GC-MS、GC-MS-MS分析表明,塔河17区块各油藏原油饱和烃、芳烃生物标志物组成和稳定碳同位素特征具有同源性,即塔河原油来自相同的烃源灶（或烃源层）。然而,对原油物性特征、成熟度特征、生物降解特征及原油馏分碳同位素组成等的深入研究发现,塔河原油属于早、晚两次充注成藏:早期充注为一般成熟型原油,相当于Ro值0.70%0.80%成熟阶段的生排烃产物,遭受生物降解后具有重、稠油特征,主要分布在4、6、7区下奥陶统储层中;晚期成藏原油相当于Ro值0.80%1.00%的高成熟阶段的生烃产物,表现为正常油、甚至轻质油或凝析油,在塔河油田广泛分布,并可以与早期充注油藏原油的生物降解残留成分进行充分混合,呈现复杂的地球化学特征。