微量元素锰对底栖甲藻热带库里亚藻(Coolia tropicalis)生长及叶绿素荧光特性的影响

以热带库里亚藻(Coolia tropicalis)为研究对象, 在不同锰浓度(0、1、5、10、50μmol·L ^-1)的人工海水培养15d, 利用叶绿素荧光动力学技术研究了其生长和光合作用对不同锰环境的响应。结果表明: 1)比生长速率(μ)和最大相对电子传递速率(rETR_max)与锰浓度均呈指数关系且对锰胁迫具有相同程度的响应; 2)锰浓度至少大于1μmol·L ^-1才能维持热带库里亚藻正常的光合作用活性, 当锰浓度低于该浓度时, 光合作用活性(F_v/F_m)在6d后开始下降, 而单位反应中心吸收光能(ABS/RC)和热能耗散(DI₀/RC)升高; 两个反应中心之间的电子传递(φ_E0)及生长并未受影响, 表明此阶段锰缺乏只影响活性光反应中心数量并提高热耗散途径; 当锰缺乏延长至15d时, 胁迫作用显现(F₀上升)并且电子传递(φ_E0)和生长受到抑制, 这阶段锰缺乏使光反应中心关闭且电子传递受阻; 3)锰缺乏的修复损伤比(r/k)并未降低, 表明锰缺乏并未影响热带库里亚藻的光保护能力。     

桑沟湾扇贝养殖对甲藻数量的影响

研究了1983-1984年和1999-2000年桑沟湾的扇贝养殖区内、外甲藻密度的变化。结果表明：扇贝养殖区内甲藻细胞的绝对密度和相对密度均低于养殖区外;在扇贝存养生物量高且摄食活跃的8月,扇贝养殖区内浮游植物的主要优势种也由甲藻变为硅藻。这表明桑沟湾的扇贝养殖对于甲藻的密度有一定的控制作用。     

南海甲藻卡氏前沟藻的生长特性研究

从海南省三亚海域收集分离了甲藻卡氏前沟藻Amphidinium carterae藻种,并在实验室成功进行了人工培养,同时对其生长特性进行了研究。实验结果表明,接种初始的细胞密度对其生长率和最高细胞密度影响很小,实验室培养和大量养殖可以采取低密度接种;最适宜培养的环境条件盐度为34,温度为26℃,营养盐浓度为2K。在温度为18—30℃、盐度为26—42、K培养液浓度系列为1/16—2 K的条件下都可存活,最大指数期平均生长率为0.958分裂.d-1,获得的最大细胞密度为7.27×105cells.ml-1。实验结果与属温带的胶州湾同物种对比,表明该藻种生长特性与温带品系有明显差异。     

Constraining Holocene sea‐surface conditions in the south‐western Black Sea using dinoflagellate cysts

This paper presents two dinoflagellate cyst records from the south‐western shelf of the Black Sea. A new site, MAR05‐13, from the Sakarya shelf is described and placed into context with site MAR02‐45, ～250 km distant on the Thracian shelf. The records provide a centennial resolution of surface water conditions in the Holocene. Analysis of the data suggests that the surface salinity of the south‐western shelf increased in a gradual and progressive manner. In the period ～11 000–9000 cal a BP the assemblages suggest surface‐water salinities between 7–13 psu. The initial arrival of euryhaline species, ～8100 cal a BP, is linked to the reconnection of the Black Sea and Marmara Sea. The suggested surface water changes related to the reconnection took approximately 1000 years. Following this initial change in assemblages, a further increase in the number of euryhaline species is noted between 5000 and 4000 cal a BP. This is linked to the establishment of more saline surface‐water conditions, close to present‐day values. The record for MAR05‐13 highlights the complexity of the changes in cyst assemblages during the mid‐Holocene. Copyright © 2012 John Wiley & Sons, Ltd.     

Distribution of dinoflagellate cysts in Yellow Sea sediments

To investigate the distribution,abundance,and species composition of dinoflagellate cysts in the Yellow Sea,surface sediment samples were collected at 37 sites,including the Korean dump site.Twenty-one dinoflagellate cyst taxa were identified,with the assemblages dominated mainly by Spiniferites bulloideus,Operculodinium centrocarpum,and cyst of Alexandrium catenella/tamarense type.A high frequency of O.centrocarpum in the Yellow Sea was observed for the first time,and it is likely that this can be attributed to the dynamics of the Yellow Sea Cold Water Mass and the Changjiang(Yangtze) River runoff.Total cyst concentrations ranged from 23 to 48 442 cysts/g dry weight,and high cyst concentrations were recorded adjacent to the dumping site.This result suggests that anthropogenic activities such as ocean dumping stimulate the growth of dinoflagellates in the Yellow Sea,which in turn leads to high levels of dinoflagellate cyst production.     

海洋寄生性甲藻——阿米巴藻Amoebophrya spp.的研究进展

寄生性甲藻阿米巴藻Amoebophrya是一类广泛寄生于纤毛虫类、放射虫类、甲藻类等海水浮游生物的原生生物,在北大西洋、北太平洋和地中海等营养盐丰富、宿主密度较高的河口和近海水环境中普遍存在,是海洋浮游食物网的重要组成部分。这类寄生性甲藻能够特异性感染海洋浮游甲藻,在有害藻华(harmful algal bloom,HAB)的发生过程中起下行控制作用,将逃脱了浮游动物摄食的浮游植物补充到微食物环(microbial loop)中去。Amoebophrya在近海海洋生态系统中的独特作用日益受到国际上越来越多研究者的关注和重视,并逐渐成为国际上海洋微型生物研究的新热点之一。近年来,已有初步调查研究表明这类寄生性甲藻在我国近海海域广泛存在;然而,目前我国尚缺乏该类寄生性甲藻的相关研究。本文系统综述了国际上该类寄生性甲藻的研究进展,针对目前研究中存在的问题并结合我国有害藻华发生机制相关研究的现状做出了分析和展望,以期推动我国该类寄生性甲藻的相关研究,为进一步阐释寄生性甲藻等海洋微型生物在有害藻华消长过程和海洋微食物环中的作用奠定基础。     

Norwegian sea‐surface palaeoenvironments of marine oxygen‐isotope stage 3: the paradoxical response of dinoflagellate cysts

High‐resolution marine palynological data have been obtained from two very long sediment cores (MD952009 and MD952010) retrieved from the southern Norwegian Sea. The dinoflagellate cyst assemblages show pronounced fluctuations in composition, which correlate strongly with magnetic susceptibility records and also mimic the δ¹⁸O signal of the GISP2 Greenland ice‐core. If focusing on the period from 48 to 30 cal. kyr BP, this correlation suggests a paradoxical response of the sea‐surface environments to the atmospheric conditions over Greenland: when the Greenland δ¹⁸O signal reflects warm interstadial conditions, the Norwegian Sea depicts cold sea‐surface temperatures with quasi‐perennial sea‐ice cover (based on dinoflagellate cysts). In contrast, when the Greenland δ¹⁸O records cold stadial periods, the Norwegian Sea‐surface temperatures are warm (based on dinoflagellate cysts), probably linked to inflow of the North Atlantic Drift. These results, similar in both cores, are contrary to those of previous studies and shed light on a possible decoupling of Norwegian sea surface‐water conditions and atmospheric conditions over Greenland. This decoupling could be linked to an atmosphere–ocean system behaving similar to that which the Northern Hemisphere is experiencing at present, i.e. strongly variable owing to the North Atlantic Oscillation. Copyright © 2002 John Wiley & Sons, Ltd.     

济阳坳陷古近纪沟鞭藻分子化石的分布与控制因素

济阳坳陷古近纪沟鞭藻化石十分丰富,沟鞭藻为有机质富集层的形成作出了重要贡献。三芳甲藻甾烷和甲藻甾烷是沟鞭藻及其祖先在古代沉积物中两种重要的存在形式,并且它们几乎专一性地由沟鞭藻提供。研究发现,沟鞭藻的不同属种,德弗兰藻属、多刺甲藻属、渤海藻类等,均可以提供丰富的三芳甲藻甾烷和甲藻甾烷,但是三芳甲藻甾烷和甲藻甾烷对沉积环境的变化具有不同的响应。在淡水、半咸水、咸水等不同水介质条件下,三芳甲藻甾烷指数稳定分布在0.50～0.96之间,高丰度的三芳甲藻甾烷与地层中丰富的沟鞭藻化石相一致,因而三芳甲藻甾烷是指示沟鞭藻输入的有效分子化石。甲藻甾烷的丰度与古沉积环境存在密切关系,高盐环境有利于甲藻甾烷的形成和保存,随着盐度的降低甲藻甾烷指数在0.04～0.74这样一个较大的范围内变化,其发育程度与有机质沉降过程中和成岩作用早期遭受的微生物降解作用有关,强烈的生物降解不利于甲藻甾烷的保存。     

Seasonal and sub-seasonal oxygen and nutrient fluctuations in an embayment of an eastern boundary upwelling system: St Helena Bay

Seasonal, sub-seasonal and spatial fluctuations in bottom dissolved oxygen (DO) were examined in St Helena Bay, South Africa’s largest and most productive embayment, between November 2013 and November 2014. Alongshore bay characteristics were assessed through comparison of variables along the 50-m depth contour. A mean coefficient of variation of 0.35 provided a measure of the relative variability of near-bottom DO concentrations along this contour. Consistently lower DO concentrations in the southern region of the bay in summer and autumn are attributed to enhanced retention. Across-shelf transects captured the seasonal development of hypoxia in relation to the distribution of phytoplankton biomass. Exceptional dinoflagellate blooms form extensive subsurface thin layers preceding the autumn DO minima in the south of the bay, prior to winter ventilation of the bottom waters. The seasonal decline in DO concentrations in the bottom waters was marked by sub-seasonal events of hypoxia, and ultimately anoxia linked to episodic deposition of organic matter, as indicated by increases in bottom chlorophyll-a concentrations. Seasonal changes in bottom water macronutrient concentrations followed trends in apparent oxygen utilisation (AOU), both of which mirrored DO concentrations. In the south of the bay, nitrogen loss through denitrification/anammox in suboxic waters was indicated by a dissolved inorganic N deficit in the bottom waters, which was most pronounced in autumn.     

Pfiesteria shumwayae (Pfiesteriaceae) in New Zealand

Pfiesteria shumwayae Steidinger et Burkholder is now known to be present in New Zealand and occurs in estuaries around the country. The presence of Pfiesteria was initially determined by a polymerase chain reaction (PCR)‐based detection assay, using oligonucleotide primers targeted at ribosomal DNA extracted from estuarine water and sediments. Presence was confirmed by isolation from fresh sediments in the presence offish (Oreochromis mossambicus), followed by identification by scanning electron microscopy. The New Zealand isolates of P. shumwayae were ichthyotoxic in bioassays, but there is no historic evidence offish kills in New Zealand associated with the dinoflagellate.