固定化氮循环细菌修复城市湖泊水体脱氮效果及N2O排放

利用从镇江金山湖天然水体中筛选分离出的土著氨化、亚硝化、硝化和反硝化细菌,将氮循环细菌固定化后在金山湖示范工程区进行水体脱氮净化效果研究.结果表明,运行一段时间后水质明显得到改善,氨氮、亚硝酸盐氮、硝酸盐氮以及总氮浓度均显著下降,氨氮浓度指标达到国家水质Ⅰ类标准,总氮浓度指标达到水质Ⅱ类标准.同时,对湖泊水体的N_2O气体排放通量进行了检测,结果显示,随着实验的进行,金山湖区的N_2O气体排放通量逐渐升高,4月份的平均值为23,51μg/(m~2·h)、5月份的平均值为29.52μg/(m~2·h)、6月份的平均值为59.10μg/(m~2·h).水质氮素指标以及N_2O气体排放通量数据说明利用微生物固定化载体强化净化技术对城市湖泊水质净化具有显著效果和重要意义.     

添加固体碳源对垂直流人工湿地污水处理效果的影响

以垂直流人工湿地小试系统为研究对象,探讨了不同位置(表层、上层、中层和下层)添加固体碳源对系统氮、磷及COD_Cr去除效果的影响.结果表明:湿地下层硝态氮去除率低于中层,最适宜碳源添加位置为垂直流人工湿地中、下层.添加碳源系统中,碳源添加位置为表层的系统COD_Cr去除率最高,各系统出水COD_Cr浓度均低于进水,不引起系统出水中COD_Cr浓度的增加.添加碳源显著提高脱氮除磷效果,碳源添加位置为下层的系统TN去除率最高,碳源添加位置为表层的系统氨氧化作用明显,出水铵态氮浓度最高,各系统对亚硝态氮和硝态氮去除率差异不明显,但对硝态氮都表现出良好的去除效果.碳源添加位置为下层的系统硝化作用最完全,TP去除能力也显著优于其他各系统.添加碳源至垂直流人工湿地下层可以达到同步脱氮除磷的效果.     

自组装膜进样质谱系统及其在砂质沉积物异化硝酸盐还原研究中的应用

沉积物中的异化硝酸盐还原过程对于海洋氮循环起着至关重要的作用。基于¹⁵N标记的培养技术是目前测定沉积物异化硝酸盐还原的主要手段。准确快速测定¹⁵N标记的产物(²⁹N₂、 ³⁰N₂)是量化异化硝酸盐还原各个过程速率的关键。本研究自行组装膜进样质谱系统用于²⁹N₂和³⁰N₂的测定,对其测量条件进行了优化。结果表明,进样蠕动泵进样流速0.80 mL/min,进样时间3～3.5 min,恒温槽温度20～25℃,同时铜还原炉温度在300～600℃的条件下,²⁹N₂/²⁸N₂和³⁰N₂/²⁸N₂的测试精密度分别可以控制在0.1%和1%以内,比较适合²⁹N₂和     

夏季长江河口潮间带反硝化作用和N2O的排放与吸收

采用培养箱乙炔抑制和现场静态箱法,于夏季(7月)在长江河口潮滩潮间带进行了采样,研究表明,长江河口潮滩水体自身N2O产生速率很低,在潮汐淹没期沉积物是上覆水体N2O的来源,其来自沉积物中反硝化、硝化等氮素循环的多个反应过程,沉积物中N2O自然产生速率在0.10～8.50μmol/(m2·h)之间,反硝化速率在21.91～35.87μmol/(m2·h)之间。退潮出露期中潮滩是大气N2O的排放源犤交换速率在-11.03～13.17μmol/(m2·h)之间犦,5～10cm地温是影响N2O排放速率的显著性因素;低潮滩-大气界面N2O排放、吸收速率在-5.75～0.49μmol/(m2·h)之间。总体上看,中潮滩是大气N2O的排放源;而低潮滩对大气N2O有明显的吸收作用。潮滩植被(海三棱草和底栖藻类)的光合作用明显抑制了N2O的排放并可能导致吸收,而其呼吸作用则增加了N2O的排放,潮间带-大气界面N2O的排放和吸收与CO2的排放、吸收有显著的正相关关系。     

反硝化增强去除乙醇对多孔介质渗透性的影响

随着乙醇混合汽油的不断推广应用,乙醇将成为地下水中与苯、甲苯、乙苯及二甲苯的同分异构体（BTEX）共存的一种新型污染物。通过4 个含水砂柱实验,研究了乙醇存在及其强化去除对含水介质渗透性能的影响。结果表明：在有限溶解氧与反硝化增强修复条件下,乙醇去除率达92% 以上;生物过程对介质渗透能力影响程度随乙醇初始浓度、消耗速率与补充频率而变化：乙醇初始浓度接近1 000 mg/L 和3 000 mg/L 时,乙醇消耗快,补充频率高,渗透系数下降总体上有连续性,最大下降幅度达一个数量级（×10-1 cm/s）;乙醇初始浓度达到5 000 mg/L 时,渗透性下降显著,可下降两个数量级,但乙酸的积累可影响生物活性,并使得渗透性变化出现反复;当不含乙醇时,汽油溶解组分对介质渗透性能的影响相对不明显。     

Nitrogen Removal in Wastewater Irrigation Systems and Its Influence on Groundwater Pollution

The experiment included ten soil columns and field investigation in 1-2 year duration. Data on the columns continuously flooded with waste water indicated that when total input of NH_4-N reached to above 70％ of the NH_4-N adsorption capacity in soil the breakthrough would appear in the output . Adequate removal of nitrogen from the waste water would require at least 170 cm deep groundwater table . Fine textured soil would promote denitrification . The columns simulating discontinuous waste water irrigation indicated that denitrification existed only in the partial microenviroument of reduction . Groundwater table depth had no strong influence on nitrogen removal . The investigation in field revealed that the groundwater recharged with waste water was not polluted by nitrogen when the aeration profile was in finer textures owing to the combined contribution of nitrification and denitrification.     

Nitrate loss and transformation in 2 vegetated headwater streams

The processes of nitrate removal from the stream waters of 2 headwater catchments were studied. In study stretches where the stream channels were vegetated with thick mats of grass (Gly‐ceria fluitans), nitrate removal processes were particularly active. In such grassed stream channels, c. 75% of the nitrate removal was attributable to plant uptake and the remainder to denitrifica‐tion. Both of these nitrate removal processes were linearly dependent on nitrate concentration, resulting in an exponential decline of nitrate level from the springs along the stream's length. Regeneration of nitrogen, by plant decay within the stream channel, results in export of dissolved organic nitrogen and paniculate nitrogen from the catchments. The overall impact of vegetated stream channels in modifying nitrogen exports from catchments is discussed.     

Upper Water Column Nitrous Oxide Distributions in the Northeast Subarctic Pacific Ocean

This is the first study to investigate the magnitude and distribution of N₂O concentrations along the Line P oceanographic transect in the Northeast (NE) subarctic Pacific Ocean. Concentrations of N₂O were measured from the surface to 600 m depth at five stations between 126°W and 145°W. Although nitrification within the mixed layer may produce some N₂O, we conclude that mixing and diffusion processes, which vertically transport N₂O upwards from below the mixed layer, are the primary sources of N₂O to the surface waters of the NE subarctic Pacific Ocean. Below the mixed layer, nitrification appears to be the dominant source of N₂O, and based on correlations of excess N₂O (ΔN₂O) versus apparent oxygen utilization and NO₃ ^? concentrations, we estimated that the N₂O yield from nitrification was approximately 0.028 to 0.040%. The longitudinal distributions of N₂O concentrations below the mixed layer were variable and we consider the potential role that different transiting water masses may play in contributing to this variability. Finally, we estimated that the regional average sea-to-air N₂O flux was 4.37 mol of N₂O km^?2 d^?1, a value which is approximately four times that of the global average seawater-to-air flux rate. Our N₂O yield estimates are within the range of those expected under oxic conditions, leading us to conclude that decreasing dissolved O₂ concentrations in the NE subarctic Pacific Ocean, and the water masses that influence this region, over the past 50 years have yet to produce a substantial increase in N₂O production. Given the expectation that dissolved O₂ concentrations in the subarctic Pacific Ocean will continue to decrease during this century, this study has provided an important baseline from which future studies will be able to track changes in seawater N₂O concentrations and fluxes to the atmosphere.

[Traduit par la rédaction] La présente étude est la première à s'intéresser à la valeur et à la distribution des concentrations de N₂O le long du transect océanographique de la ligne P dans le Pacifique Nord-Est subarctique. Les concentrations de N₂O ont été mesurées de la surface jusqu’à une profondeur de 600 m à cinq stations entre 126°O et 145°O. Bien que la nitrification à l'intérieur de la couche de mélange puisse produire du N₂O, nous concluons que le mélange et les processus de diffusion, qui transportent verticalement le N₂O vers le haut à partir d'en dessous de la couche de mélange, sont les sources principales de N₂O pour les eaux de surface du Pacifique Nord-Est subarctique. En dessous de la couche mélange, la nitrification semble être la principale source de N₂O, et d'après les corrélations de l'excès de N₂O (ΔN₂O) par rapport à l'utilisation apparente d'oxygène et aux concentrations de NO₃ ^?, nous avons estimé que le rendement en N₂O de la nitrification était approximativement de 0,028 à 0,040%. Les distributions longitudinales des concentrations de N₂O en dessous de la couche de mélange étaient variables et nous considérons le rôle possible que des différentes masses d'eau transitoires peuvent avoir à jouer dans cette variabilité. Finalement, nous avons estimé que la moyenne régionale du flux mer–air de N₂O était de 4,37 moles de N₂O km^?2 j^?1, une valeur qui est environ quatre fois celle du taux planétaire moyen du flux eau de mer–air. Nos estimations du rendement en N₂O sont de l'ordre de celles attendues dans des conditions oxyques, ce qui nous amène à conclure que la diminution des concentrations d'oxygène dissout dans le Pacifique Nord–Est subarctique, et dans les masses d'eau qui influencent cette région, au cours des cinquante dernières années ont encore à produire une augmentation substantielle de production de N₂O. Étant donné qu'on s'attend à ce que les concentrations d'oxygène dissout dans le Pacifique subarctique continuent à diminuer durant le présent siècle, cette étude a fourni une importante base de référence à partir de laquelle de futures études pourront suivre les changements dans les concentrations de N₂O dans l'eau de mer et dans ses flux vers l'atmosphère.     

~(15)N示踪法测定海洋反硝化速率中~(29)N_2浓度的准确定量

利用15 N示踪法实测南海水体反硝化速率的研究发现,培养水样在长时间密闭放置过程中也会受到外界空气的污染,且其29N2/28N2比值恒定为0.007 35。根据空气背景中29N2/28N2比值恒定的特征,提出基于质量平衡关系校正空气N2污染的方法,通过将样品实测29N2浓度扣除由外界空气贡献的29N2浓度,可获得由生物反硝化作用所产生的29N2准确浓度,进而可计算出准确的反硝化速率。经空气29N2背景校正后,29N2浓度的偏差明显小于未经校正的结果,且29N2浓度与培养时间之间的线性相关性显著加强,凸显出空气29N2背景校正是获取准确反硝化速率的关键。鉴于15 N示踪法已被广泛应用于海洋水体与沉积物反硝化速率的测定中,所提出的空气29N2背景校正方法具有重要的意义。     

Rates and regulation of nitrogen cycling in seasonally hypoxic sediments during winter (Boknis Eck,SW Baltic Sea): Sensitivity to environmental variables

This study investigates the biogeochemical processes that control the benthic fluxes of dissolved nitrogen (N) species in Boknis Eck – a 28 m deep site in the Eckernförde Bay (southwestern Baltic Sea). Bottom water oxygen concentrations (O_2−BW) fluctuate greatly over the year at Boknis Eck, being well-oxygenated in winter and experiencing severe bottom water hypoxia and even anoxia in late summer. The present communication addresses the winter situation (February 2010). Fluxes of ammonium (NH₄⁺), nitrate (NO₃⁻) and nitrite (NO₂⁻) were simulated using a benthic model that accounted for transport and biogeochemical reactions and constrained with ex situ flux measurements and sediment geochemical analysis. The sediments were a net sink for NO₃⁻ (−0.35 mmol m⁻² d⁻¹ of NO₃⁻), of which 75% was ascribed to dissimilatory reduction of nitrate to ammonium (DNRA) by sulfide oxidizing bacteria, and 25% to NO₃⁻ reduction to NO₂⁻ by denitrifying microorganisms. NH₄⁺ fluxes were high (1.74 mmol m⁻² d⁻¹ of NH₄⁺), mainly due to the degradation of organic nitrogen, and directed out of the sediment. NO₂⁻ fluxes were negligible. The sediments in Boknis Eck are, therefore, a net source of dissolved inorganic nitrogen (DIN = NO₃⁻ + NO₂⁻ + NH₄⁺) during winter. This is in large part due to bioirrigation, which accounts for 76% of the benthic efflux of NH₄⁺, thus reducing the capacity for nitrification of NH₄⁺. The combined rate of fixed N loss by denitrification and anammox was estimated at 0.08 mmol m⁻² d⁻¹ of N₂, which is at the lower end of previously reported values. A systematic sensitivity analysis revealed that denitrification and anammox respond strongly and positively to the concentration of NO₃⁻ in the bottom water (NO₃⁻_BW). Higher O_2−BW decreases DNRA and denitrification but stimulates both anammox and the contribution of anammox to total N₂ production (%R_amx). A complete mechanistic explanation of these findings is provided. Our analysis indicates that nitrification is the geochemical driving force behind the observed correlation between %R_amx and water depth in the seminal study of Dalsgaard et al. (2005). Despite remaining uncertainties, the results provide a general mechanistic framework for interpreting the existing knowledge of N-turnover processes and fluxes in continental margin sediments, as well as predicting the types of environment where these reactions are expected to occur prominently.