6-磷酸山梨醇脱氢酶(gutD)基因克隆、测序和表达

本研究根据6-磷酸山梨醇脱氢酶基因(gutD)两端序列设计引物，以假单胞杆菌(Pseudonmonas XM)的总DNA为模板通过PCR的方法克隆gutD基因并进行序列分析，将克隆得到的gutD基因与原核表达载体pBV220连接并转化大肠杆菌(Escherichia coli)JM101后检测其蛋白表达及菌株生长耐盐性。     

动态基准实时测量系统的开发与应用

介绍了动态基准实时测量系统的硬件组成、站点上全站仪棱镜组合安装与检测方法、系统软件的开发及特点，最后对系统在广州地铁某地铁站结构变形自动监测中的应用情况进行了阐述。通过实际应用表明该系统能应用在自动化、高精度的变形监测领域。     

地层学的发展推动油气地质理论研究的进步

自上个世纪50年代以来,地层学经历了彻底的变革,逐渐由“相模式”、“沉积体系”、“地震地层学”发展到目前的“层序地层学”,形成高分辨率层序地层学和事件地层学的理论体系和研究方法。文章围绕层序地层学、高分辨率层序地层学基本方法及理论体系,与含油气系统相关关系进行了讨论。     

Two-Dimensional Mathematical Model of Tidal Current and Sediment for Oujiang Estnary and Wenzhou Bay

A 2-D mathematical model of tidal current and sediment has been developed for the Oujiang Estuary and the WenzhouBay. This model accomodates complicated features including multiple islands, existence of turbidity, and significant differ-ence in size distribution of bed material. The governing equations for non-uniform suspended load and bed load transport arepresented in a boundary-fitted orthogonal curvilinear coordinate system. The numerical solution procedures along with theirinitial conditions, boundary conditions, and movable boundary technique are presented. Strategies for computation of thecritical condition of deposition or erosion, sediment transport capacity, non-uniform bed load discharge, etc. are suggested.The model verification computation shows that, the tidal levels computed from the model are in good agreement with the fielddata at the 18 tidal gauge stations. The computed velocities and flow directions also agree well with the values measuredalong the totally 52 synchronously observed verticals distributed over 8 cross sections. The computed tidal water throughputsthrough the Huangda‘ao cross section are close to the measured data. And the computed values of bed deformation fromYangfushan to the estuary outfall and in the outer-sea area are in good agreement with the data observed from 1986 to 1992.The changes of tidal volumes through the estuary, velocities in different channels and the bed form due to the influence of thereclamation project on the Wenzhou shoal are predicted by means of this model.     

Hydrocarbon Potential of Pre-cenozoic Strata in the North Yellow Sea Basin

The North Yellow Sea Basin ( NYSB ), which was developed on the basement of North China (Huabei) continental block, is a typical continental Mesozoic Cenozoic sedimentary basin in the sea area. Its Mesozoic basin is a residual basin, below which there is probably a larger Paleozoic sedimentary basin. The North Yellow Sea Basin comprises four sags and three uplifts. Of them, the eastern sag is a Mesozoic Cenozoic sedimentary sag in NYSB and has the biggest sediment thickness; the current Korean drilling wells are concentrated in the eastern sag. This sag is comparatively rich in oil and gas resources and thus has a relatively good petroleum prospect in the sea. The central sag has also accommodated thick Mesozoic-Cenozoic sediments. The latest research results show that there are three series of hydrocarbon source rocks in the North Yellow Sea Basin, namely, black shales of the Paleogene, Jurassic and Cretaceous. The principal hydrocarbon source rocks in NYSB are the Mesozoic black shale. According to the drilling data of Korea, the black shales of the Paleogene, Jurassic and Cretaceous have all come up to the standards of good and mature source rocks. The NYSB owns an intact system of oil generation, reservoir and capping rocks that can help hydrocarbon to form in the basin and thus it has the great potential of oil and gas. The vertical distribution of the hydrocarbon resources is mainly considered to be in the Cretaceous and then in the Jurassic.     

Influence of large-diameter pipe pile driving on surrounding marine soils and adjacent submarine pipelines

Abstract

With the large-scale development and utilization of ocean resources and space, it is inevitable to encounter existing submarine facilities in pile driving areas, which necessitates a safety assessment. In this article, by referring to a wharf renovation project as a reference, the surrounding soil response and buried pipe deformation during pile driving in a near-shore submarine environment are investigated by three-dimensional (3D) numerical models that consider the pore water effect. Numerical studies are carried out in two different series: one is a case of a single pile focusing on the effect of the minimum plane distance of the pile–pipe, and the other is a case of double piles focusing on the effect of the pile spacing.     

台湾海峡及其西边地区正常地震动态及危险性特征

本文从地震的时，空分布特征，区域应力场动态，能量释放方式，ｂ值及震群特征６个方面研究了台湾海峡及其西边地区地震活动的正常动态及异常特征。结果表明，具有前兆意义的变化模式表现为区域地震活动在时间，空间及功能方面的有序性变化，即：（ａ）地震空间分布由分散转为集中，形成条带或空区；（ｂ）断裂活动由多组转为单一，应力场趋向一致；（display status     

Tectonic Significance of the Taitung Canyon, Huatung Basin, East of Taiwan

Since the beginning of formation of Proto-Taiwan, the subducting Philippine (PH) Sea plate has moved continuously through time in the N307° direction with respect to Eurasia (EU), tearing the EU plate. The subducting EU plate includes a continental part in the north and an oceanic part in the south. The boundary B between these two domains corresponds to the eastern prolongation of the northeastern South China Sea ocean-continent transition zone. In the Huatung Basin (east of Taiwan), the Taitung Canyon is N065° oriented and is close and parallel to B. Seismic profiles show that the southern flank of the canyon corresponds to a fault with a normal component of a few tens of meters in the sediments and possible dextral shearing. Several crustal earthquakes of magnitude >%6 are located beneath the trend of the Taitung Canyon and focal mechanisms suggest that the motion is right-lateral. Thus, faulting within the sedimentary sequence beneath the Taitung Canyon is a consequence of underlying dextral strike-slip crustal motions. As the continental part of the EU slab located north of B has been recently detached, some subsequent dextral strike-slip motion might be expected within the EU slab, along the ocean-continent transition zone, which is a potential zone of weakness. We suggest that the dextral strike-slip motion along the ocean-continent boundary of the EU slab might trigger the observed dextral strike-slip motion within the overlying PH Sea crust and the associated faulting within the sediments of the Huatung Basin, beneath the Taitung Canyon. An erratum to this article is available at .     

土工布充砂袋的应用及其研究进展

对土工布充砂袋技术和国内外的应用进行了介绍。鉴于土工布充砂袋属于一种新兴起的土工新材料和新技术，在设计和工程实际中还存在着许多急待解决的问题，着重对各种典型状态和典型环境下的土工布充砂袋的变形、应力和土工布充砂袋堆的稳定性研究进展进行综述，最后提出进一步研究的思路。