Profiling Planctomycetales diversity with reference to anammox‐related bacteria in a South China Sea,deep‐sea sediment

A systematic assessment of Planctomycetales diversity in a South China Sea, deep‐sea sediment (1657 m) was conducted using the 16S rRNA gene analysis approach. PCR amplification of the samples from seven sediment layers (0.1, 1, 3, 5, 7, 9 and 11 m below the surface sediment) using the primer set Pla‐46‐F/1392‐R showed that the Planctomycetales existed within a limited range of sediment depths (≤ 5 m), and had a decreasing trend in diversity with increasing depth. The majority of the retrieved Pla‐46‐F/1392‐R sequences belonged to Pirellula‐related Planctomycetales, and two sequences retrieved from the 0.1‐m layer (GenBank accession numbers: DQ996944 and DQ996945 ) shared the same anammox‐related signature oligonucleotides and were closely related to commonly recognized anammox organisms. To identify new anammox‐related biomarkers, three primer sets were designed for amplifying the fragments of hydroxylamine oxidoreductase and S‐adenosylmethionine radical enzyme genes, but no related sequences were found. Our multiple 16S rRNA gene primer sets (Journal of Rapid Methods and Automation in Microbiology, 2008, in press) revealed even an higher diversity of Planctomycetales in the 0.1‐m layer of the sediment, especially at genus level. Our data profiled the distribution pattern of Planctomycetales diversity along sediment depths, and provided molecular evidence for the existence of anammox‐related bacteria in a new location, which broadens our understanding of Planctomycetales diversity in deep sea sediments.     

The influence of light availability and predatory behavior of the decapod crustacean Nephrops norvegicus on the activity rhythms of continental margin prey decapods

The day–night cycle is one of the strongest geophysical cycles modulating species' behavioral rhythms. However, in deep-water continental margins, where light intensity decreases over depth, interspecific competition may alter behavioral responses to day–night cycles. The burrowing decapod crustacean Nephrops norvegicus is a large-size predator in benthic communities, exerting despotic territorial behavior. In this study, we analysed how the effect of light intensity cycles on decapod behavioral rhythms is reduced as one moves from shelves to slopes. In the Western Mediterranean, the predatory behavior and interspecific competition for substrate use of Nephrops increases moving from the shelf (100–110 m) to the slope (400–430 m). Vector fitting and generalized additive models were used to assess the effect of light intensity and behavioral rhythms of N. norvegicus on the temporal variation of prey decapods co-occurring in trawl tow catches carried out on the shelf and the slope during October 1999 and June 2000. The combination of diel variations in light intensity and N. norvegicus abundance influences the activity rhythms of prey decapods in a depth- and seasonal-dependent manner. Light modulation is stronger on the shelf and weaker on the slope, where Nephrops population size is greater. Although present regression analysis does not necessarily imply a direct cause–effect relationship between rhythms of predators and prey, we suggest that Nephrops alters the temporal patterning in the behavior of its prey on the slope, where light intensity is reduced. This alteration is stronger in endobenthic species than in benthopelagic species; the former rely on bottom substrate for the expression of behavioral rhythms, experiencing stronger interspecific competitions with Nephrops at time of activity.     

基于融合U-Net及ConvLSTM的海面高度异常预报方法研究

海面高度异常是反映海洋环境状况的主要变量之一。本文使用1993—2019年的融合月均海面高度异常数据,建立了基于深度学习的海面高度异常预测神经网络模型,提出了基于融合U型网络(U-Net)和卷积长短记忆网络(ConvLSTM)的中长期海面高度异常预报模型。在研究海域0.25°×0.25°的空间分辨率下,模型测试集预报结果的均方根误差和平均绝对误差分别为0.039 m和0.027 m,均优于全连接LSTM预报模型和ConvLSTM+CNN预报模型,为大中尺度的海面高度异常预报提供了新的方法。     

西太平洋马里亚纳海沟附近海山的金相柳珊瑚属(珊瑚虫纲:软珊瑚目:金柳珊瑚科)二新记录种

为探究热带西太平洋海山生物多样性,2016年利用"发现"号遥控无人潜水器(ROV)对西太平洋马里亚纳海沟附近的M2海山进行了采样调查,其中获得了3个深水金相柳珊瑚样本。通过光学显微镜和扫描电镜观察,鉴定其为黑发金相柳珊瑚Metallogorgia melanotrichos(WrightStuder,1889)和长刺金相柳珊瑚Metallogorgia macrospina Kükenthal,1919。这两种金相柳珊瑚均为该区域的新记录种,对其作了详细的形态描述,并依据特征性状对该属所有物种作了分类检索。     

Cement Deep Soil Mixing (CDSM) for Solidification of Soft Estuarine Sediments

A preliminary study was conducted to determine the potential for cement deep soil mixing (CDSM) technology as a method for in-situ solidification of contaminated river and estuarine sediments. The study was conducted in Newark Bay, near the mouth of the Passaic River, New Jersey. The primary objective of the study was to evaluate the viability of CDSM for the in-situ S/S with a focus on: 1) determining the correct mix of the cement slurry, which provides rapid stabilization of the sediment matrix, 2) potential resuspension of solids during CSDM operations, 3) the effects of high organic content on the solidification process, and 4) the feasibility of using conventional dredging/extraction methods once the sediments have been stabilized and allowed to cure. The results of the study show CDSM slurry mixtures, as low as 7% in cement content, result in significant solidification and strength gain of in-situ sediments under ambient conditions. In sediments with very high organic contents (> 20%), the slurry mix would need to be adjusted to account for retardation effects of organics on cement hydration. Sediment resuspension during application was shown to be minimal at a distance of as little as 75 feet from the mixing head. Strength gains were considerable, effectively consolidating the sediment particles in a secure matrix, but not so high as to preclude extraction of solidified sediments with conventional dredging equipment. Dredged solidified sediment exhibited characteristics of a stiff glacial clay, and as such was easier to handle and transport than untreated dredged sediments. This technique has high potential to be used as an interim remedial measure prior to either extraction and decontamination/disposal or proper capping.     

大深度载人潜水器低速大漂角模糊滑模航向控制研究

通过模型试验测量大深度载人潜水器低速大漂角运动时所受到的非线性水动力。基于一种新的模糊滑模控制策略,为潜水器设计了鲁棒航向控制器。在不同的漂角子区间内分别设计局部镇定的滑模控制器,然后通过Takagi-Sugeno模糊推理系统将它们光滑连接,得到模糊滑模控制。仿真计算结果充分显示了该控制策略的有效性。     

华南花岗岩型铀矿床成矿机理研究进展

本文介绍了华南花岗岩型铀矿床地质特征,系统总结了前人对其成矿热液来源、物质来源,铀的迁移沉淀机制、碱交代及花岗岩与成矿的关系等方面的研究成果。指出铀源体中的晶质铀矿及富铀矿物在深部相对还原的环境中被氧化而进入成矿热液中起主要作用的可能是大量幔源F和放射性衰变诱发产生的氧及碱交代溶蚀作用,热液中的大部分U6 主要被S2-和Fe2 等还原剂在浅部相对氧化的环境中还原成矿;给出了一种较简易的物质来源定量方法和铀成矿模式。     

新一轮就矿找矿

本文简要回顾了二十世纪五、六十年代就矿找矿的历史,进而提出新一轮的就矿找矿。相比之下,新一轮就矿找矿具有新的特点。深部找矿实则是一种就矿找矿,地壳连续成矿理论、深部流体(成矿)作用理论、成矿台阶理论都为新一轮就矿找矿提供了理论依据。深部找矿在国内外初显成效,湘中地区亦有很多成功案例。本文从3个方面总结了深部找矿的一些经验标志,即矿种及矿种组合系列标志、花岗岩成矿专属性标志、幔源成因矿床的鉴别标志。此外,辨别成矿流体的深(幔)源标志、加强成矿规律研究、更新观念、引进新技术新方法,都是实际深部找矿工作中需要注意的。     

深层砂泥岩储层继承性裂缝发育特征

西部挤压盆地内低渗性含油气砂泥岩储层构造运动期次多、埋藏深度大、泥质夹层多、高角度网状缝继承性发育,裂缝不仅是油气的有效运移通道,也是储层压裂开发效果的主要影响因素。选择西部天山山前某区块深层砂泥岩裂缝性气藏为研究区,以裂缝形成机制的探讨和研究为突破点,在岩心观察、CT层析成像扫描、成像测井解释的基础上,精细统计裂缝产状、开度、充填度,结合流体包裹体及古应力场划分裂缝发育期次,最后总结继承性裂缝的发育模式。研究结果表明,砂泥岩储层裂缝继承性缝的有利发育条件为多期次构造运动、低应力差、低逼近角度、适当泥质含量或低摩擦因数,岩性是内因,构造应力是外因。     

新型复合胶无粘土冲洗液研究及应用

针对松散覆盖层、强风化破碎层取心率低,岩心松散破碎,孔壁稳定性差等问题,进行了以天然植物胶为主原料的处理剂复配改性研究和冲洗液优化配方研究。主要研究方法是通过有机聚合物和无机交联剂的作用,对羟丙基瓜尔胶(GRJ)进行交联改性,以此获得新型复合胶无粘土冲洗液优化配方,并进行了现场应用。应用效果表明,该冲洗液密度低,粘度可调范围大,流变性和润滑性好,成膜护心、护壁作用强,完全满足第四系覆盖层、胶结差的砂卵石层和强风化破碎地层取心钻进要求。