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31.
麻痹性贝毒(Paralytic Shellfish Poisoning,PSP)毒素由石房蛤毒素(saxitoxin,STX)及其衍生物组成,目前己发现20余种,在赤潮研究、分子生物学和神经生物学基础研究、医药、军事防化等方面都有应用潜力[其结构、类型和应用见本集刊王云峰等(2003)“麻痹性贝毒毒素的应用研究进展”一文]。由于PSP毒素的稀有来源和国际社会对STX交易的禁止,限制了国内PSP毒素应用及研究的全面深入展开,因此本文作者对PSP毒素进行了制备,并采用不同方法对制备的PSP毒素进行了研究。
PSP毒素能够选择性地可逆抑制可兴奋膜的电压依赖钠离子通道的开放,从而阻止神经冲动的发生和传导,使神经、肌肉丧失兴奋性(Frace et al.,1986;Penzotti et al.,1998)。本文利用神经束膜下记录和全细胞膜片钳技术,报道了从塔玛亚历山大藻(Alexandrium tamarense)中提取的PSP粗毒素对小鼠运动神经末梢膜电流和NG108-15细胞钠离子通道的作用研究结果,并与STX标准毒素的作用结果进行了比较。
NG108-15细胞是由小鼠神经母细胞瘤和大鼠胶质细胞瘤融合的杂交细胞,经分化剂分化后,显示诸+L1196如兴奋性、合成和释放乙酰胆碱、与培养肌细胞形成突触联系等多种神经细胞的基本特性(Hamprcht,1977)和Na+、K+、Ca2+等多种离子通道,已作为神经细胞模型被广泛应用于分析药物对离子通道作用的研究(Enomoto et al.,1992; Docherty et al.,1992;Shi et al.,1993;Hu et al.,1997a;Hu et al.,1997b);发育出具有Na+内流支持的锋电位(Hamprecht,1977),该电位是分析作用于膜钠离子通道药物的好材料。 相似文献
32.
We have examined wind-induced circulation in the Sea of Okhotsk using a barotropic model that contains realistic topography
with a resolution of 9.25 km. The monthly wind stress field calculated from daily European Centre for Medium-Range Weather
Forecasting (ECMWF) Re-Analysis data is used as the forcing, and the integration is carried out for 20 days until the circulation
attains an almost steady state. In the case of November (a representative for the winter season from October to March), southward
currents of velocity 0.1–0.3 m s−1 occur along the bottom contours off the east of Sakhalin Island. The currents are mostly confined to the shelf (shallower
than 200 m) and extend as far south as the Hokkaido coast. In the July case (a representative for the summer season from April
to September), significant currents do not occur, even in the shallow shelves. The simulated southward current over the east
Sakhalin shelf appears to correspond to the near-shore branch of the East Sakhalin Current (ESC), which was observed with
the surface drifters. These seasonal variations simulated in our experiments are consistent with the observations of the ESC.
Dynamically, the simulated ESC is interpreted as the arrested topographic wave (ATW), which is the coastally trapped flow
driven by steady alongshore wind stress. The volume transport of the simulated ESC over the shelf reaches about 1.0 Sv (1
Sv = 106 m3s−1) in the winter season, which is determined by the integrated onshore Ekman transport in the direction from which shelf waves
propagate.
This revised version was published online in July 2006 with corrections to the Cover Date. 相似文献
33.
To study ice-induced vibration of a compliant conical structure,a series of model tests were performed from 2004 to 2005.In the tests,the ice sheet before the compliant conical structure was found to fail in two-time breaking.From 2005 to 2006,this type of ice failure was studied through more groups of tests.The tests show that two-time breaking is the typical failure of ice before steep conical structures,and is controlled by other factors at the same time,such as ice speed and the angle of the cone. 相似文献
34.
A study of sea surface wave propagation and its energy deformation was carried out using field observations and numerical experiments over a region spanning the midshelf of the South Atlantic Bight (SAB) to the Altamaha River Estuary, GA. Wave heights on the shelf region correlate with the wind observations and directional observations show that most of the wave energy is incident from the easterly direction. Comparing midshelf and inner shelf wave heights during a time when there was no wind and hence no wave development led to an estimation of wave energy dissipation due to bottom friction with corresponding wave dissipation factor of 0.07 for the gently sloping continental shelf of the SAB. After interacting with the shoaling region of the Altamaha River, the wave energy within the estuary becomes periodic in time showing wave energy during flood to high water phase of the tide and very little wave energy during ebb to low water. This periodic modulation inside the estuary is a direct result of enhanced depth and current-induced wave breaking that occurs at the ebb shoaling region surrounding the Altamaha River mouth at longitude 81.23°W. Modelling results with STWAVE showed that depth-induced wave breaking is more important during the low water phase of the tide than current-induced wave breaking during the ebb phase of the tide. During the flood to high water phase of the tide, wave energy propagates into the estuary. Measurements of the significant wave height within the estuary showed a maximum wave height difference of 0.4 m between the slack high water (SHW) and slack low water (SLW). In this shallow environment these wave–current interactions lead to an apparent bottom roughness that is increased from typical hydraulic roughness values, leading to an enhanced bottom friction coefficient. 相似文献
35.
More and more researches show that neither the critical downward acceleration nor the critical slope of water waves is a universal constant. On the contrary, they vary with particular wave conditions. This fact moders the models either for the probability of wave breaking B or for the whitecap coverage W based on these criteria difficult to apply. In this paper and the one which follows we seek to develop models for the prediction of both B and W based on the kinematical criterion. First, several joint probabihstic distribution functions (PDFs) of wave characteristics are derived, based on which the breaking properties B and W are estimated. The estimation is made on the assumption that a wave breaks ff the horizontal velocity of water particles at its crest exceeds the local wave celerity, and whitecapping occurs in regions of fluid where water particles travel faster than the waves. The consequent B and W depend on wave spectral moments of orders 0 to 4.Then the JONSWAP spectrum is used to represent the fetch-limited sea waves in deep water, so as to relate the probahility of wave breaking and the whitecap coverage with wind parameters. To this end, the time-averaging technique proposed by Glazman (1986) is applied to the estimation of the spectral moments involved, and furthermore, the theoretical models are compared with available observations collected from published literature. From the comparison, the averaging time scale is determined. The final models show that the probability of wave breaking as well as the whitecap coverage depends on the dimensionless fetch. The agreement between these models and the database is reasonable. 相似文献
36.
根据Taylor的湍流扩散理论和Hay、Pasquill提出的拉格朗日流和欧拉流的相关函数的相似性,用黄河三角洲埕北海域的实测资料,按四种不同方案计算出该海域的水平扩散系数和谱密度分布。指出:该海区潮流扩散系数主要受潮流的影响,其分布特征与潮流的分布基本一致,所求的水平扩散系数主要是由周期性的潮运动给予潮流扰动所致。方案4是通过富氏变换滤波消去了周期性潮流扰动,本方案计算的水平扩散系数是由周期为6h以下的小尺度涡提供能量。 相似文献
37.
An instrument which measures the velocity and direction of benthic water currents at depths of up to 80 m is described. Characteristics of this meter include: (i) readings are not affected by water flow during sinking or retrieval; (ii) it is portable; (iii) it operates unattended; (iv) it is inexpensive; and (v) it requires only simple maintenance. A timing circuit which operates two time periods in succession is used to activate and de-activate a revolution counter and a fluidfilled compass which are mounted on a special frame and vane system. In the field, the instrument accurately measured average current velocities of up to 6 m s−1. Data are presented which illustrate the usefulness of this instrument in describing the velocity and direction of benthic currents along the east coast of Australia and demonstrate a positive correlation between benthic current velocity and catch rates of the commercially-exploited spanner crab Ranina ranina. 相似文献
38.
在连云港近岸海域计算潮流场基础上建立拉格朗日余流模型,并对连云港市两大堤建成前后的拉格朗日余流变化进行了分析,且选择有代表性的排污口进行了数值跟踪。 相似文献
39.
Long Waves Associated with Bichromatic Waves 总被引:1,自引:0,他引:1
DONG Guohai 《中国海洋工程》2001,(2):281-289
A numerical model of low frequency waves is presented. The model is based on that of Roelvink (1993), but the nu-merical techniques used in the solution are based on the so-called Weighted-Average Flux (WAF) method with Time-Operator-Splitting (TOS) used for the treatment of the source terms. This method allows a small number of computational points to be used, and is particularly efficient in modeling wave setup. The short wave (or primary wave) energy equation is solved with a traditional Lax-Wendroff technique. A nonlinear wave theory is introduced. The model described in this paper is found to be satisfactory in modeling low frequency waves associated with incident bichromalic waves. 相似文献
40.