Cloning, Expression and Characterization of Translationally Controlled Tumor Protein （TCTP） Gene from Flatfish Turbot （Scophthalmus maximus）

A full-length cDNA encoding translationally controlled tumor protein of marine flatfish turbot (Scophthalmus maximus), SmTCTP, was isolated with rapid amplification of cDNA Ends (RACE). SmTCTP consisted of a 5' untranslated region (UTR) of 84 bp, a 3' UTR of 451 bp and an open reading flame (ORF) of 513 bp, encoding a protein of 170 amino acid residues, which contained two signature sequences of TCTP family. The 5'UTR of SmTCTP started with a 5'-terminal oligopyrimidine tract (5'-TOP), a typical feature for translationaily controlled mRNAs. The deduced amino acid sequence of SmTCTP was similar to the other known verte-brate TCTPs in a range of 58.8% to 64.1%. The length offish TCTPs was diverse among species, e.g., TCTP of turbot and sea perch (Lateolabrax japonicus) is 170 aa in length, while that of zebrafish (Danio rer/o) and rohu (Labeo rohita) is 171 aa in length. North-ern blot analysis revealed that SmTCTP has only one type of mRNA. Its expression level in albino skin was slightly higher than that in normal skin. We constructed the pET3Oa-SmTCTP expression plasmid. The recombinant protein of His-tag SmTCTP was over-expressed in E. coli, purified and identified with peptide mass fingerprinting. These results may pave the way of further inves-tigation of the biological function of TCTP in fish.     

双向地震波作用下巨子型有控结构建筑的隔震设计

为了增强巨子型有控结构建筑的动力特性,提升其稳定性,设计双向地震波作用下建筑有控结构。采用3种磁流变阻尼器(MRD)与滑移隔震混合控制结构构成单体建筑有控结构,其包括巨结构和子结构,并建立该有控结构的动力分析模型。在动力分析模型中输入水平和竖向地震,得到模型的竖向和水平滑动状态运动微分方程,依据这两个方程采用自适应模糊神经网络优化动力分析模型,构建优化模型。从优化模型出发,通过实例实验分析得出,优化设计双向地震波作用下建筑有控结构时,在其上部结构层间和隔离层各安装一个MRD,可确保优化设计后的有控结构在不同双向地震工况下的地震反应控制效果最佳,且有控结构在双向地震工况2下,结构第一层、中间三层以及顶层的加速度和位移的时程曲线走向一致,且差距微小;同时有控结构的巨结构顶层侧移响应随着子结构刚度增加而提高,动力特性没有明显的变化,子结构随着其自身刚度增加顶层侧移响应表现稳定,子结构动力特性增强。     

工程勘探瞬变电磁仪关键技术研究

瞬变电磁勘探法是工程勘探领域里新兴的一种很有前途的测量方法。但是作为一种重要探测工具,瞬变电磁仪的许多关键技术还没有解决,限制了瞬变电磁勘探法在工程勘探领域的应用。本文研究了瞬变电磁仪的硬件和软件设计中的关键技术,并提出了程控接收天线法和早期信号检测法等。      

黔中早二叠世茅口晚期断裂陆缘层序地层分析

黔中早二叠世晚期发育变异多姿的硅质岩－碳酸盐岩和火山岩，笔者通过大地构造学、火山岩岩石学和岩石地球化学、层序地层学的综合分析认为，与一般的被动大陆边缘不同，该区这种断裂大陆边缘的层序地层特征主要依据纵向上反映海平面变化的岩性突变来识别，而岩性突变又是构造变动的反映。据此，将该区茅口组二段划分为一个准层序组和两个准层序，每个准层序以突变最大海泛面为基础，包含了凝缩层和加积高位体系域；横向上解释为破裂碳酸盐台地型层序地层格架，即把构造作用与层序地层格架的形成以及沉积体系的分异统一起来，提出了断裂陆缘层序层分析的实例。     

新疆马庄山金矿控矿地质条件及其成因浅析

华力西期次火山活动及同熔岩浆侵入活动为马庄山金矿提供了中低温含金热液，前寒武纪结晶基底，石炭系白山组英安质凝灰岩及石英斑岩（次火山岩）为矿源层，横贯矿区的两条北东主干断裂（Ｆ３１２，Ｆ３１３）为导矿构造，次火山岩内张性断裂及主干断裂派生的张扭性断裂为主要容矿，控矿构造，今后找矿工作中应侧重深部找矿。     

我国煤储盖层的成因类型、时空展布及控气特征

基于含煤岩系沉积研究的最新成果，将我国煤储盖层划分为浅海－障壁海岸、浅海－无障壁海岸、三角洲、河流、湖泊和冲积扇六种基本成因类型，并探讨其时空展布规律和对煤层气资源赋存的影响，指出碎屑海岸和三角洲型有利于煤层气的保存。     

陕西省水磨坪——尖峰垭锑金矿资源富集区矿床特征及找矿前景

通过对该区成矿地质条件、矿带矿体产出特征、矿石质量及类型，热液活动和围岩蚀变情况的分析研究，总结出该区的矿产分布规律，控矿因素及找矿标志，指出了今后找矿的重点地段。     

Mechanism of tracer blasting

Summary A variety of overbreak control techniques are used during excavation with the drill and blast system. Tracer blasting is used in Canadian underground mines to minimize blast damage and involves placing a low-strength detonating cord along the length of a blast hole prior to charging with ammonium nitrate-fuel oil (ANFO). The results of tracer blasting are not always consistent and its mechanism is only hazily comprehended. In the absence of a clearly defined mechanism, it is difficult to analyse the results of tracer blasting and to identify the factors responsible for the inconsistency of results.A series of bench blasts and pipe tests were carried out to investigate the mechanism of tracer blasting. The evidence indicated partial deflagration and desensitization of ANFO, thus reducing the total available explosive energy. The rock mass surrounding the traced blasthole experienced a low level of ground vibrations. As a result of the continuous side initiation of ANFO, energy partitioning was more in favour of gas energy. A mechanism of tracer blasting has been proposed and the factors responsible for the inconsistency of the results have been identified in this paper.     

黑火药控制爆破在花岗岩石材开采中的应用

介绍了黑火药控制爆破法在河南省商城县花岗岩开采中的试验与应用，给出了工作面开采要素和合理的爆破参数。