Methods for extraction of microorganism DNA from glacier surface snow

In order to thoroughly investigate the diversity of glacier microorganisms, four DNA extraction methods with different lysis patterns were tested and two screened methods (the Bosshard-Bano method and the Zhou method) were optimized for the most effective form of the filter membrane (cut vs. uncut), the DNA extraction method, and the precipitation method. The two optimized methods were then compared with the commercial Mo-Bio DNA extraction kit, and the results showed that the kit was generally suitable for extraction of microorganism DNA from glacier surface snow. Procedurally, it was found that a modified Bosshard- Bano method (i.e., cutting the filter membrane into pieces, using a specific lysis pattern [lysozyme (5 mg/mL)-protease K (1 mg/mL)-CTAB (1%)-SDS (1%)], performing the extraction only once by chloroform-isoamyl alcohol (24:1), and conducting DNA precipitation by pure ethanol) was also an effective and less expensive method for extraction of microorganism DNA from glacier surface snow.     

北京市郊区传统村落价值评价及可持续利用模式探讨——以北京市门头沟区传统村落的调查研究为例

北京市西部门头沟区保留有许多传统村落,这些传统村落经过历史的积淀而具有典型的地域特征和深厚的文化底蕴,成为京西独特的景观旅游资源。在深入系统调查的基础上,结合悠久性、完整性、乡土性、协调性、典型性对传统村落的价值特征进行了综合评价,将传统村落划分为遗产性村落、特色性村落和保护性村落三种类型。在探讨传统村落可持续利用机制的基础上总结了传统村落可持续利用的6种模式。     

贵州乌蒙山区资源相对承载力的时空动态变化

贵州乌蒙山区是国家划定的18片贫困区之一,随着人口增长和社会经济发展的加速,山区人地资源矛盾日趋突出,已严重阻碍了乌蒙山区社会经济的可持续发展。运用并改进了相对资源承载力的研究思路和计算方法,研究了1995~2006年贵州乌蒙山区自然资源、经济资源、生活水平的相对承载力和综合承载力的时空动态变化过程。对于认清和把握乌蒙山区资源承载力变化规律,完善山区资源环境承载力研究成果具有重要指导意义。研究表明:贵州乌蒙山区人口严重超载,而承载人口的主要资源为土地资源,经济资源和生活水平承载力偏低;相对资源承载力空间差异显著,绝大多数县域综合承载力均处于超载状态。     

SCALING TECHNIQUES IN THE ANALYSIS OF ENVIRONMENTAL COGNITION DATA∗

The utility of nonmetric, multidimensional-scaling techniques is demonstrated for the analysis and collection of environmental-cognition data. By comparing the multidimensional-scaling solutions of a real-setting map to scaling solutions for sketch maps and two psychophysical, distance-scaling procedures, we demonstrate that magnitude estimation of actual interpoint distances is comparable in accuracy to sketch maps when produced without constraints, or when subjects are given a specified list of landmarks to include on their maps. Triadic comparisons of actual interpoint distances were less accurate than the three other techniques.     

黄河北岸兰州段丘陵区土壤生态化学计量与空间变异北大核心CSCD

为探究黄河北岸兰州段丘陵区土壤碳、氮、磷含量及化学计量比的空间变异特征,基于12个样地数据,采集1 m剖面内不同发生层土壤进行土壤含水率(SWC)、pH、有机碳(SOC)、全氮(STN)和全磷(STP)的测定。分析了研究区土壤碳、氮、磷含量及其生态化学计量特征在植被和土层上的差异及空间变异特征。结果表明:(1)研究区SOC、STN、STP均值分别为4.53、0.74、0.13 g·kg^(-1),C∶N、C∶P、N∶P均值分别为7.85、55.17、8.40;(2)研究区SOC、STN、C∶N和N∶P的块基比C_(0)/(C_(0)+C)<0.25,主要受气候、植被、地形等自然因素影响;STP、C∶P则C/(C+C)>0.75,主要受退耕还草等人为因素影响;(3)研究区土壤整体受到氮限制,与此同时有机碳更为匮乏,磷则在短期内不会成为限制因子。在黄河流域兰州段北岸丘陵区北岸的生态治理与恢复中,应重视有机肥和氮肥的配置与施加,同时需减少人为干扰,这对于植被的快速恢复与养分的固定具有重要意义。     

中国登陆热带气旋与太平洋海表温度的关系

根据1951～2000年中国登陆热带气旋与厄尔尼诺年、拉尼娜年的关系分析,得到了一些重要认识：(1) 在厄尔尼诺年,中国登陆热带气旋数偏少;(2) 厄尔尼诺事件强度越大,中国登陆热带气旋数越少;(3) 厄尔尼诺事件的结束时间若超过5月1日,那么次年中国登陆热带气旋数偏少;(4) 中国登陆热带气旋数的特多年主要集中在拉尼娜年;(5) 在拉尼娜次年,中国登陆热带气旋数偏少;(6) 中国登陆热带气旋强度越大,在厄尔尼诺年出现机会越少;(7) 在厄尔尼诺年,中国初旋偏迟,终旋偏早。同时对其机理进行了探讨。     

“Greatest lake period” and its palaeo-environment on the Tibetan Plateau

The “greatest lake period” means that the lakes are in the stage of their maximum areas. As the paleo lake shorelines are widely distributed in the lake basins on the Tibetan Plateau, the lake areas during the “greatest lake period” may be inferred by the last highest lake shorelines. They are several, even tens times larger than that at present. According to the analyses of tens of lakes on the Plateau, most dating data fell into the range of 40-25 ka BP, some lasted to 20 ka BP. It was corresponded to the stage 3 of marine isotope and interstitial of last glaciation. The occurrence of maximum areas of lakes marked the very humid period on the Plateau and was also related to the stronger summer monsoon during that period.     

广东省不动产登记审批发证系统的设计与实现

在广东省进一步加快推进不动产统一登记发证的背景下,通过对业务需求进行系统分析,提出了系统的设计思路、总体框架和数据库结构设计,并对系统在实现过程中采用的关键技术进行了分析,然后,介绍了系统重要功能的实现,最后对系统的主要特点进行了总结和展望。     

甘肃省“五化”协同发展时空演变格局及影响因素分析

以2005年、2010年和2015年甘肃省87个县区、县级市及自治县的工业化、城镇化、信息化、农业现代化与绿色化（简称“五化”）为研究对象,利用空间自相关分析、地理加权回归分析等空间分析方法,结合构建的“五化”协同发展模型,对甘肃省“五化”协同发展的时空分异格局、时空演变趋势和影响因素进行研究分析,以期为甘肃省各市州科学的制定发展政策提供参考。结果表明：甘肃省“五化”协同发展格局具有明显的空间相关性且空间差异显著,协同水平呈现出明显的西北高、东南低分布格局;“五化”发展水平和综合水平较低,协同水平总体不高,以轻度失调、濒临失调和勉强协同为主,但总体上升幅度较大;“五化”协同发展的影响因素,按其影响力大小依次为：农村居民人均可支配收入 > 城乡居民可支配收入差 > 固定资产投资总额 > 财政支出 > 地形起伏度 > 城乡居民消费差 > 降水量。