Construction and characterization of a fosmid library for pathogenic bacterium <Emphasis Type="Italic">Vibrio anguillarum</Emphasis>

Vibrio anguillarum is a common bacterial pathogen in fish.However,little is known about its pathogenic mechanism,in part,because the entire genome has not been completely sequenced.We constructed a fosmid library for V.anguillarum containing 960 clones with an average insert size of 37.7 kb and 8.6-fold genome coverage.We characterized the library by end-sequencing 50 randomly selected clones.This generated 93 sequences with a total length of 57 485 bp covering 1.4% of the whole genome.Of these sequences,58...     

GIS符号库通用数据模型及其数据交换格式的研究

Symbol portrayal is an important function of GIS.Sharing symbolic information in different GIS platforms is necessary for GIS applications and users.This paper discusses the necessity,possibility and solution technique of sharing a symbol library in different GIS platforms.The route map is designed as follows:first,to set up a general data model for the symbol library,then to design a standard exchange format,and finally to call on the GIS manufacturer to provide the interchange tools for their symbol libra...     

皱纹盘鲍(Haliotis discushannai Ino)胚胎RNA分离和cDNA文库构建

从皱纹盘鲍胚胎样本中分离RNA并构建了cDNA文库。分别收集孵化8、10和12h的皱纹盘鲍胚胎,用抽滤除菌的海水反复悬浮胚胎,将这3个发育阶段的胚胎样本等量混合后用TRIZOL试剂提取总RNA,将提取物用酚氯仿异戊醇再抽提2次,分离获得高质量的总RNA。从总RNA中分离mRNA,构建了皱纹盘鲍混合胚胎的cDNA文库,未扩增胚胎文库的滴度为5.0×106pfu/ml,重组率为94.12%,插入片段均大于400bp,70.6%克隆的插入片段分布在1000—1500bp之间,扩增后的文库滴度为3.06×109pfu/ml。     

STUDY ON GAMETOPHYTE VEGETATIVE GROWTH OF UNDARIA PINNATIFIDA AND ITS APPLICATIONS

When cultured under certain environmental coriditions (25℃, light intensity 80 μmol/m~2 ·s, LD12/12, in enriched seawater medium with 7 × 10~(-4)mol/L NO_3,-N, 1.56× 10~(-4)mol/L, PO_4-P and supplementsof other elements like Mn, Fe, I, etc.), mate and female gametophytes of U pinnatifida keptgrowing vegetatively and propagated fast at average daily fresh weight increase rate of about 20%. Theempirical formula G_m=G_o. 3~m was established to estimate the output of vegeative gametophytes. Vigorousvegetative gametophyte cells began to form reproductive structures (oogonium and spermatangium, whenthe tmperature was lower than 25℃ and other environmental factors wrre kept optimal. The sufficientsupply of gametophyte cells provided enough seeds for raising Undaria sporelings on prodiction scale.Controlled cross-breeding experiments using selcted male and female gametophyte clones which increasetheir cell number by mitosis instead of meiosis were also carried out in vitro. Juvenile sporophytes fromthe cro     

基于高分辨率遥感目标特征库的地震灾情快速评估方法

高分辨率遥感影像已逐步成为地震灾害快速评估的主要数据源之一,但现有的遥感地震灾害信息提取方法存在对研究人员目视解译经验依赖性强和利用高分辨率影像提取结果精度不高的问题。因此,本文提出了一种基于目标特征库的高分辨率遥感灾害信息快速提取方法,用于提升遥感影像灾害信息提取的效率和自动化程度,并对基于目标特征库进行地震灾情快速评估的几项关键技术（目标特征库构建、样本匹配方法和自动分类方法）进行了阐述,最后,以云南鲁甸地震龙头山镇地区为研究区,基于高分辨率遥感影像在目标特征库支持下开展了地震灾情快速评估实验。通过与灾后调查数据的对比分析发现,基于高分辨率遥感灾害目标特征库的地震灾情快速评估结果在精度上可以满足灾情快速评估的业务需要,同时还具有更好的时效性。     

尼罗罗非鱼pik3r1基因的克隆和mRNA表达分析

【目的】哺乳动物pik3r1基因参与多种免疫途径,探索pik3r1基因在罗非鱼(Oreochromis)中的作用。【方法】克隆尼罗罗非鱼(Oreochromis niloticus)pik3r1(命名为On-pik3r1)cDNA全长,对该基因进行生物信息学分析,并运用荧光定量PCR方法分析无乳链球菌(Streptococcus agalactiae)刺激后On-pik3r1 mRNA在各组织种的表达模式。【结果与结论】On-pik3r1基因编码区2190 bp,编码729个氨基酸,5′端非编码区(UTR)为542 bp,3′UTR为2248 bp,理论分子质量为83.99 ku,等电点为5.73。On-pik3r1与斑马拟丽鱼(Maylandia zebra)相似性最高(98.53%),与其他物种的同源性在70%以上,表明pik3r1在物种进化过程中高度保守。On-pik3r1在健康尼罗罗非鱼各组织中均有表达,在肌肉中表达量最高,其次是鳃、皮肤,在胸腺中表达量最低。经灭活无乳链球菌刺激后,On-pik3r1表达量在肠道、鳃、脾脏、头肾、脑部等5个组织中均极显著下调(P<0.01),在胸腺中表现为4 h时极显著下调(P<0.01),24、48、72 h时为显著下调(P<0.05)。On-pik3r1参与了罗非鱼的对无乳链球菌的免疫应答过程。     

基于COM组件式定量遥感模型库的实践

为了使遥感模型库具有良好的可扩展性、重用性和互用性 ,本文提出了组件式模型库的设计思路和总体框架 ,并通过实例验证了遥感波谱模拟的可行性和采用 COM组件开发定量遥感模型库的可实施性。     

草鱼种质退化相关SRAP分子标记的筛选

采用相关序列扩增多态性(sequence-realted amplified polymorphism,SRAP)技术分析野生草鱼和家养草鱼,并筛选与草鱼种质退化相关的分子遗传标记。共进行88对引物组合的检测,产生标记数目共计905个。依据标记在群体中出现的频率和变化规律,共筛选出21个可能与种质相关的特异性标记,对这些特异性标记进行测序并将测序结果进行BLAST分析,发现测得片段中有8个片段能在GeneBank中找到同源性较高的序列,而其他片段与数据库中序列的相似性较低。     

高校图书馆人力资源与队伍建设

在分析广东海洋大学图书馆人力资源与队伍现状的基础上，提出了改善高校图书馆人力资源现状的一些举措；创新理念，创新机制；加大改革力度和图书馆职业准入制度和劳动合同制，建立健全人员培训制和激励机制等。     

基于互联网的地理信息变化监测关键技术研究

针对目前地理空间信息变化更新体系存在投入大、时效性差等问题,提出了基于互联网的地理空间变化监测的技术体系,利用互联网本体大、实时性高等优点,设计了地理信息检索词典库和检索算法,实现了从海量互联网信息中提取地理信息变化监测信息,为地理空间信息的更新提供了有效的来源。