Application of the first internal transcribed spacer(ITS-1) of ribosomal DNA as a molecular marker to population analysis in farrer''s scallop Chlamys farreri

Sequence variation of the first internal transcribed spacer of ribosomal DNA （ ITS - 1 ） was examined and its application to the study of genetic variation was explored in four populations of farter＇ s scallop Chlamys farreri. ITS - 1 fragments, with a length of about 300 bp,of 78 individuals collected from Dalian, Qingdao, Yantai in China and Korea respectively were amplified via PCR, cloned and sequenced. Intra-genomic variation was examined by sequencing several clones of single individuals. Alignment and polymorphism analysis detected 44 haplotypes and 50 polymorphic sites which consist of 30 substitutions and 20 indels, indicating a high level of polymorphisms. Sequence analysis also showed a very low level of intra-individual variation. All these features validated the feasibility of application of ITS - 1 fragment to population analysis. Polymorphism analysis showed that the Korea sample has the richest genetic variation, followed by Yantai and Qingdao samples. AMOVA （analysis of molecular variance） showed that the majority （96.26%） of genetic variation was distributed within populations and 3.74% resulted from among populations, but with P 〈 0.05 （ = 0.042）, indicating that the populations in this study have significant divergence. This output was basically concordant with the result arising from RAPD data and different from that from mitochondrial 16S rDNA sequence data. Discussion on this inconsistency was made accordingly.     

一种腐屑食性鱼类对生态系统氮循环的影响

研究表明 :腐屑食性鱼类——梭鱼对环境中有机氮的摄入能力随体重的增加而增加 ,随温度的升高 ,体重系数显著减少。摄入氮 (CN,mg/ d)与体重 (W,g)和温度 (T,℃ )的数值关系可用如下模型表示 :CN=0 .6 370 W1 .0 642 ln(3.70 0 8T)。体重为 1 0 0 g的梭鱼每日可消耗环境中 332 mg氮有机质。梭鱼氮的吸收效率主要受温度的影响 ,而氮的转化效率与体重呈显著的正相关关系。随体重的增加 ,排泄氮所占比例降低     

Experimental Study on Stability of Breakwaters with Penetrating Box Foundations

1 .ＩｎｔｒｏｄｕｃｔｉｏｎＴｈｅｐｅｎｅｔｒａｔｉｎｇｂｕｃｋｅｔａｎｄｂｏｘｆｏｕｎｄａｔｉｏｎｉｓａｎｅｗｔｙｐｅｏｆｓｔｒｕｃｔｕｒｅ ,ｗｈｉｃｈｈａｓｂｅｅｎａｐｐｌｉｅｄｉｎｍａｎｙｅｎｇｉｎｅｅｒｉｎｇｆｉｅｌｄｓ .Ｉｎｖｉｅｗｏｆｔｈｅｈｉｇｈｃｏｎｓｔｒｕｃｔｉｏｎｃｏｓｔａｎｄｔｈｅｆｏｒｍｉｄａｂｌｅｃｏｎｓｔｒｕｃｔｉｂｉｌｉｔｙｏｆｏｆｆ ｓｈｏｒｅｐｌａｔｆｏｒｍｓｗｉｔｈｐｉｌｅｄｆｏｕｎｄａｔｉｏｎｓ ,Ｂａｅｒｈｅｉｍｅｔａｌ.(1 990 )ｐｒｏｐｏｓｅｄｔｈｅｃｏｎｃ…     

对虾暴发性流行病及其预防研究

１９９３～１９９４年我国沿海养殖对虾发生了特大的暴发性流行病，损失惨重。本文报道该病的特点、症状及危害，并在患病的中国对虾、日本对虾、长毛对虾的中肠、肝胰腺的上皮细胞核和细胞质及腹肌纤维间质中发现大量具有囊膜的杆状病毒。通过病毒引起宿主靶组织细胞的病理变化、无包涵体等特性分析，认为该病毒属于有囊膜杆状病毒科Ｃ亚群，不同于已知的对虾所有杆状病毒，为对虾的一类新致病病毒。文中还分析了主要致病原因，通过调查和试验提出８条有针对性的预防措施。     

龙须菜和扁江篱多糖的组成及其抗肿瘤效果

于１９８５－１９８７年期间用冷水和热水对采集于青岛的龙须菜和扁江篱的琼胶型多糖进行提取。两海藻的热水提取多糖都通过ＤＥＡＥ－ＳｅｐｈａｄｅｘＡ５０色谱桩用热水和不同浓度ＮａＣｌ深液前后进行洗脱分级。龙须菜和扁江篱多糖的主要级分分别为０．５ｍｏｌ／Ｌ和１．０ｍｏｌ／ＬＮａＣｌ洗脱级分。对各级分做化学分析及ＩＲ和＾１３ＣＮＭＲ光谱分析。结果表明，龙须菜多糖由琼二糖、琼胶糖前体和６－ＯＣＨ３－琼二糖组成     

海面对风应力的非定常响应

针对常垂直湍流系数和变垂直湍流系数两种情况，给出了开阔静止海面对风应力的非定常响应的解析表达式，并讨论了水深，风应力强弱对响应过程的影响，尽管没有考虑风场的非定常性，但本文的结果对理解海洋对风应力的非定常响应过程具有一定的帮助，对分析海面停风后的消衰过程也具有实际意义。通过与实测资料比较，认为垂直湍流系数模式要比常垂直湍流系数模式更为合理。     

抑制栉孔扇贝第一极体对受精卵染色体行为及胚胎倍性组成的影响

采用细胞松弛素B（CB）处理，栉孔扇贝（Chlamys ferreri）抑制其受精卵的第一极体（PB1），研究抑制PB1对受精卵减数分裂过程及胚胎倍性组成的影响。结果发现，抑制第一极体显著改变了受精卵的染色体行为，在第二次减数分裂过程中共发现4种典型染色体分离类型，分别是三极分离（41．7％）、二极分离（11．7％）、双二极分离（24．9％）和非同步分离（2．8％），其余的受精卵（19．0％）染色体分离行为紊乱。对4-8细胞期胚胎的倍性组成进行分析，发现处理组中含有二倍体（10．9％）、三倍体（12．5％）、四倍体（19.5％）、五倍体（12．6％）以及非整倍体（46．6％）胚胎。研究结果表明，二极分离和双二极分离分别是形成三倍体和四倍体的主要机制，而其他的染色体分离行为将主要形成非整倍体。     

台风"杜鹃"影响期间福建大风天气的特点及成因

台风“杜鹃”影响期间，福建大风天气出现的比较早，而且范围比较大．本文通过对天气尺度的环境场、具有代表性的单站资料的对比分析，寻求大风出现的原因．还深入利用MM5模式对台风的气压场、风场、流场等进行数值模拟，分析结果表明台湾地形倒槽的形成对此次大风天气出现有着重要的作用，此外从流场分型的角度进一步分析台湾地形倒槽的形成原因．     

Calculation of Environmental Capacity of Petroleum Hydrocarbon in Jiaozhou Bay

The method has been established to calculate the environmental capacity (ECo),surplus environment capacity (SECo) of water with respect to marine petroleum hydrocarbons associated with oil (PHAOs) and the self-purification capacity (SPCo) of main self-purification process to PHAOs in the Jiaozhou Bay, China, according to the dynamic model for distribution of marine PHAOs among multiphase environments. The variation of concentration of PHAOs in the Jiaozhou Bay is well simulated by the dynamic model. Based on the model.the ECo, SECo of water with respect to PHAOs in the Jiaozhou Bay were calculated during the last 10 years under the first-class and second-class quality standard requirement, according to SPCoof main self-purification process to PHAOs. The results show that about 200 tons of PHAOs could be discharged into the Jiaozhou Bay for maintaining the first class seawater quality standard, and about 600 tons of PHAOs for the second class seawater quality standard later.