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351.
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The atomic force microscopy (AFM) is a recently developed, bench-top instrument that can image the surface structures of biological
specimens at high resolution with simultaneous measurement of their size. This paper describes the application of AFM to marine
bacteria. Both natural and cultured bacteria were retained on a filter or placed on glass, washed, air-dried and observed
by AFM. The instrumental condition, the choice of suitable filter, effect of fixation and filtration, comparison with epifluorescent
microscopic (EFM) count, and the size and shape of bacterial cells were investigated. An Isopore filter was best for concentration
and subsequent observation because of its surface flatness. Cross section images showed that both rod and coccoid cells were
flattened, the former usually having a two-humped shape. Bacterial cells were differentiated from non-living particles based
on their cross section shape and size. Bacterial counts by AFM and EFM showed good agreement. Although size measurement is
easily done by the instrument, AFM tends to overestimate the size of microspheres. More work is thus needed on the size measurement
of living organisms. Because AFM easily provides images of natural bacterial cells at high magnification, it can be used as
a new tool to study the fine structures of marine bacteria.
This revised version was published online in July 2006 with corrections to the Cover Date. 相似文献
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近岸海浪模式在中国东海台风浪模拟中的应用--数值模拟及物理过程研究 总被引:6,自引:2,他引:6
较为详细地介绍了基于能量平衡方程的第三代近岸海浪数值模式SWAN(Simulation Waves Nearshore)及其包含的物理过程(风生浪、底摩擦、白浪耗散、深度诱导波破碎、非线性波-波相作用等),并利用该模式对影响杭州湾-长江口沿岸海域的一次台风浪过程进行了模拟研究:模式所需风场由藤田台风风场模型嵌入对应台风特征等压线,并对相应时段的NCAR/NCEPT资料、单站资料进行同化后提供;利用自嵌套的方式提供波谱边界条件;模式模拟的结果与实际海浪观测资料相符较好,在此基础上,研究了底摩擦、深度诱导波破碎、三波相互作用等物理过程联合对近岸台风浪的影响,初步认识了它们在近岸台风浪生成、传播过程中的重要作用。 相似文献
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Fumio Horiguchi Kisaburo Nakata Naganori Ito Ken Okawa 《Estuarine, Coastal and Shelf Science》2006,70(4):589
A risk assessment of Tributyltin (TBT) in Tokyo Bay was conducted using the Margin of Exposure (MOE) method at the species level using the Japanese short-neck clam, Ruditapes philippinarum. The assessment endpoint was defined to protect R. philippinarum in Tokyo Bay from TBT (growth effects). A No Observed Effect Concentration (NOEC) for this species with respect to growth reduction induced by TBT was estimated from experimental results published in the scientific literature. Sources of TBT in this study were assumed to be commercial vessels in harbors and navigation routes. Concentrations of TBT in Tokyo Bay were estimated using a three-dimensional hydrodynamic model, an ecosystem model and a chemical fate model. MOEs for this species were estimated for the years 1990, 2000, and 2007. Estimated MOEs for R. philippinarum for 1990, 2000, and 2007 were approximately 1–3, 10, and 100, respectively, indicating a declining temporal trend in the probability of adverse growth effects.A simplified software package called RAMTB was developed by incorporating the chemical fate model and the databases of seasonal flow fields and distributions of organic substances (phytoplankton and detritus) in Tokyo Bay, simulated by the hydrodynamic and ecological model, respectively. 相似文献
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Are invasive species most successful in habitats of low native species richness across European brackish water seas? 总被引:5,自引:0,他引:5
Marjo Paavola Sergej Olenin Erkki Leppkoski 《Estuarine, Coastal and Shelf Science》2005,64(4):738-750
European brackish water seas (Baltic Sea, Black Sea and Sea of Azov, Caspian Sea) are subject to intense invasion of non-indigenous species (NIS). In these seas, salinity is the most important range limiting factor and native species seem to reach a minimum species richness at intermediate salinities. This trend, revealed by Remane in 1934 and later on confirmed by many other scientists, was compared to the salinity range of already established NIS in the European brackish water seas. It turned out that most NIS are well adapted to the salinities holding lowest native species richness, already in their native area, and that NIS richness maximum in brackish water seas occurs in the salinity intervals of native species richness minimum. A predictable pattern in the salinity range of NIS can be used as a tool in initial risk assessment of future invasions in brackish water seas, especially when mapping highly potential donor and recipient areas. A product of empty niches, suitable environmental conditions, and availability of proper vectors might be the most effective predictor for the invasibility of brackish water areas. 相似文献
360.