Diagnosis of iridovirus in large yellow croaker by PCR

A rapid and sensitive PCR-based method for the detection of the large yellow croaker iridovirus(LYCIV) is described,which involves the amplification of a 295 bp fragment of the LYCIV ATPase gene from DNA isolated from naturally infected fish spleen.Sequencing of LYCIV ATPase gene fragment showed it shared 100% nucleotide sequence homology with the corresponding region of the ATPase gene of red sea bream iridovirus(RSIV) and sea bass iridovirus(SBIV),suggesting that LYCIV was homologous with RSIV and SBIV at least in part of the gemone.The specificity and sensitivity of the PCR procedure were tested on the iridovirus-infected fishes,the expected fragment was detected from spleen DNA samples of infected fishes,whereas no fragments were amplified from healthy fish spleen DNA,white spot syndrome baculoviruses(WSBV) DNA and pseudorabies virus(PRV) DNA.Detection limit of this method was 10^-7 ng positive plasmid DNA containing target sequence,equal to about 100 virions.In the infected experiment,first positive detection(1/4) appeared at Day 3 post-infection,all fish(4/4) tested positive at Day 7,however obvious symptoms were observed at Day 8,so LYCIV infection could be detected prior to the appearance of obvious symptoms.These results indicate that this PCR method could be used for early,rapid and specific detection of LYCIV infection.     

Toward a generic method for studying water renewal, with application to the epilimnion of Lake Tanganyika

We present a method, based on the concept of age and residence time, to study the water renewal in a semi-enclosed domain. We split the water of this domain into different water types. The initial water is the water initially present in the semi-enclosed domain. The renewing water is defined as the water entering the domain of interest. Several renewing water types may be considered depending on their origin. We present the equations for computing the age and the residence time of a certain water type. These timescales are of use to understand the rate at which the water renewal takes place. Computing these timescales can be achieved at an acceptable extra computer cost.The above-mentioned method is applied to study the renewal of epilimnion (i.e. the surface layer) water in Lake Tanganyika. We have built a finite element reduced-gravity model modified to take into account the water exchange between the epilimnion and the hypolimnion (i.e. the bottom layer), the water supply from precipitation and incoming rivers, and the water loss from evaporation and the only outgoing river. With our water renewal diagnoses, we show that the only significant process in the renewal of epilimnion water in Lake Tanganyika is the water exchange between the epilimnion and the hypolimnion, other phenomena being negligible.     

国外渔具选择性研究进展

为了保护和可持续利用渔业资源 ,渔具选择性的研究在国际上倍受重视。该文以比较作业法中渔具选择性曲线的推导 ,特别是利用扩张 SEL ECT模型推导渔具选择性曲线为例 ,介绍国外渔具选择性研究的最新进展 ,以期作为我国开展这方面研究工作的参考     

无公害刺参稳产健康养殖新技术

本文针对目前刺参养殖中存在的问题,简单明了叙述刺参无公害健康养殖一系列新技术和新方法,对推动海参养殖业持续、稳定、健康发展,起到积极作用和效果。     

一个湍能封闭模型对风暴潮海底底应力的应用研究

将一个三维湍能封闭模型应用于开阔海区的风暴潮，通过数值计算探讨了Ｔａｙｌｏｒ底摩擦二次率的拖曳系数随空间的分布及拖曳系数与水深、海底粗糙度、风向和风速等因素的关系。本文对底摩擦二次率的可靠性做了评价。     

从受训神经-模糊模型中提取知识

本文对用于模式分类、函数逼近、参数估计的多层感知器 (MLPs)给出 1个清晰的关于内部行为的解释。作者以单隐层的 MLP为例 ,论述了关于 MLP的内部行为的半线性分析理论。对受训的MLP,将隐层单元的输出分别定义为网络输出的正、负“内部分量”;定义内部分量的连接权重集为给定问题的“内部判别模式”;建立了 MLP和模糊集相结合的新模型 ;分析了 MLP的结构为 N- 2 - 1和N- H- 1 ,给出权重初始化的方法 ;提出了 1种从受训神经 -模糊模型 (NFMs)中提取知识的全新的具有实用价值的方法。     

肝素酶Ⅰ对合成的肝素五糖的酶解作用及对其抗Xa因子活性的影响

研究肝素酶Ⅰ(来源于Flavobacterumheparinase，EC4.2.2.7)对人工合成的且含有与抗凝血酶Ⅲ特定结合位点的肝素五糖(SHP)的酶解作用，并对酶解作用的动力学进行研究。利用强阴离子高效液相色谱(SAX-HPLC)对酶解混合物进行分离，利用质谱(ESIMS)和核磁共振波谱(1H-NMR)技术对得到的二糖和三糖的结构进行确证。研究结果表明，这种被作为肝素反向试剂的肝素酶Ⅰ可水解人工所合成的肝素五糖，从而使之丧失抗Xa因子活性。     

基于EMD与神经网络的机械故障诊断技术

经验模式分解 (EMD)是分析非线性、非平稳信号的有力工具 ,它将信号分解为突出了原信号的不同时间尺度的局部特征信息的内在模函数 (IMF)分量。本文通过将各 IMF分量输入到 BP网络中进行训练学习和故障诊断 ,比直接输入原信号可以提高 BP网络对故障诊断的准确率 ,而且减少了训练时间。     

鲈鱼胆汁中芘及代谢产物含量与水体中芘含量的关系

运用GC—MS的方法和荧光分光光度法，研究鲈鱼分别暴露于0．1、1．0和10．0μg／dm^3质量浓度芘溶液中7d，水体中芘及其鲈鱼胆汁中芘和1-羟基芘含量的变化,实验结果显示：(1)鲈鱼对水体中的芘具有非常显著的去除作用。(2)随着芘暴露浓度的增大，鲈鱼对芘的代谢去除作用增强。(3)随着暴露时间的延长和芘暴露浓度的增大，胆汁的1-羟基芘浓度递增。(4)鲈鱼胆汁具有较高的芘浓度，对海水中的芘具有较强的富集作用。(5)鲈鱼胆汁的1-羟基芘及芘的浓度与水体芘浓度均具有很好的相关性，对于指示水体的芘污染程度具有一致性；可作为指示水体芘污染程度的生物标志物。     

A moving PIV system for ship model test in a towing tank

A moving particle image velocimetry (PIV) system was successfully developed and used in a large towing tank for ship model tests to observe velocity fields near ship models. The experimental method involved adjustable optical devices for various test conditions and a special particle-seeding device. The streamwise and cross-streamwise flow fields of a yacht model and a tanker model were measured. Ship type, bottom shape, and towing speed were found to be the causes of problems affecting optical access and image quality. Possible solutions, deeper optical ducts, dark painting color, and pre-processed analysis method, were proposed and discussed.