高效液相色谱法测量海洋浮游植物色素浓度

JGOFS计算和SeaWiFS计划推荐高效液相色谱（HPLC）作为海洋浮游植物色色浓度的测量方法。文章参考SeaWiFS计划制定的HPLC分析规范，叙述采用反相C18ODS色谱柱、996PDA、自动进样器（未配柱温箱）和三元梯度的色谱法测量海洋浮游植物色素浓度的具体实践。     

南海北部水体叶绿素a浓度反演的生物光学模型

利用2003年至2005年秋季在南海多个航次的现场观测数据,研究了南海北部海区遥感反射率的变化,并分析了用于全球海洋叶绿素a浓度反演的OC2和OC4模型在本海区的适用性。结果表明,在南海北部海域,OC2和OC4模型高估了叶绿素a浓度,高估范围一般约在80%—200%之间,其中最高可达640%,即OC2和OC4模型并不适用于南海海域。在此基础上,根据现场实测的表观光学数据,利用遥感反射率比值(Rrs(433)/Rrs(555))与叶绿素a浓度的关系建立了两套能够精确反演南海北部海域叶绿素a浓度的本地化经验算法———算法1和算法2,并利用其对南海北部海域的叶绿素a浓度进行反演。结果表明,由本地化模型反演得到的叶绿素a浓度与实测的叶绿素a浓度具有较好的相关关系,其平均相对偏差分别为51%和53%,相关系数为0.75。     

塞曼石墨炉原子吸收法直接测定海水中的铬

海水基体对Cr有增感作用。本文研究了抗坏血酸、柠檬酸三铵、酒石酸、乙酸铵等有机基体改进剂对消除基体干扰效果。前二者均有效地消除基体干扰,但1％抗坏血酸 1％柠檬酸三铵混合试剂的效果更好,它不仅可以消除基体干扰,还可降低背景及噪声,Cr的吸收峰也较平滑。本法准确,相对偏差在0.7—4.4％范围内。回收率在92—109％范围内。若海水Cr含量低于定量下限时,可采用多次进样法。     

贻贝粉酶解液提取复合氨基酸

报道了以脱脂后的贻贝粉为原料，采用酶解法从其酶解液中分离提取复合氨基酸的工艺。其收率为２０％，纯度约为９０％。其中必需氨基酸（不计色氨酸）约占复合氨基酸总量的５５％，其氨基酸模式与ＦＡＯ／ＷＨＯ组织确定的模式及人乳模式进行了对照，通过适量添加个别氨基酸可使其配比接近人乳模式。     

Atmospheric Correction of SeaWiFS Data for the Retrieval of Suspended Sediment in East China Coastal Waters

The data of SeaWiFS (Sea-Viewing Wide Field-of-View Sensor), installed on SeaStar, has been used to generate SSC (suspended sediment concentration) of complex and turbid coastal waters in China. In view of the problems of the SeaDAS (SeaWiFS Data Analysis System) algorithm applied to China coastal waters, a new atmospheric correction algorithm is discussed, developed, and used for the SSC of East China coastal waters. The advantages of the new algorithm are described through the comparison of the restdts from different algorithms.     

大亚湾水域两种拟菱形藻的形态学鉴定及毒素分析

对2005年分离自广东大亚湾的两种潜在产毒拟菱形藻进行了形态学观察和毒性分析.通过透射电子显微镜(TEM)观察确认藻种为细弱拟菱形藻(Pseudo-nitzschia cuspidata)和多纹拟菱形藻(P.multistriata).采用高效液相色谱法(HPLC)对这两种藻的常规培养样进行软骨藻酸毒素检测,结果均未检测到软骨藻酸.鉴于以上两种拟菱形藻存在着潜在产毒特性,不同培养条件的诱导实验尚需继续.     

Investigation on the Deposition in an Excavated Trough at the East Shore of Jiaozhou Bay, China

- Based on field investigations, this paper analyzes three types of harbour basinns and navigation channel excavated on seabed in Jiaozhou Bay, get a general rule of deposition for excavated trough, it found that pollution is one of crucial factors resulting in the deposition of the excavated trough in the east shore of Jiaozhou Bay. With these results, it predicted the annual deposition thickness for the excavated trough and disclosed the fact that it can't be deposited deadly during one storm. At the same time, with two-dimensional numerical model, it studied the effects of the excavated trough and the reclamation near shore on tidal cureent and said that the excavated trough can decrease the current velocity passing through the trough about 10- 15%, but only limited inside and near the trough and there are no effect on other regions; reclamation can cut off the pollution sources and no obvious effect on the currents of the Jiaozhou Bay. Connecting the deep trough and Cangkou tidal channel with a new     

凤眼莲根对东海原甲藻生长的抑制作用及机制研究

观察凤眼莲Eichhornia crassipes根粉末及丙酮提取物对东海原甲藻Prorocentrum donghaiense生长的影响,比较分析根系丙酮提取物中的化学成分以及不同成分抑藻效果,探讨凤眼莲根对藻类生长的抑制作用及其化学基础。结果显示,1.5g.L-1以上的凤眼莲根粉末可完全抑制东海原甲藻的生长。实际浓度0.019g.L-1的凤眼莲根丙酮提取物对东海原甲藻可产生50%的抑制率。N-苯基-2-萘胺浓度为1mg.L-1时,第6天对东海原甲藻的抑制率超过60%。浓度50μl.L-1时,亚油酸对东海原甲藻的抑制率超过80%。气相色谱-质谱联用(GC-MS)或高效液相色谱(HPLC)检测显示,凤眼莲根丙酮提取物中含有一定量的亚油酸和N-苯基-2-萘胺,同时还有大量的长链脂肪酸如十六酸、9-十六碳烯酸等。结果表明,凤眼莲根可显著抑制东海原甲藻的生长,N-苯基-2-萘胺、亚油酸可能是凤眼莲根抑藻的主要因子。     

The Structure of A Turbulent Jet in A Crossflow-Effect of Jet-Crossflow Velocity

A comprehensive numerical study on the three-dimensional structure of a turbulent jet in crossflow is performed. The jet-to-crossflow velocity ratio (R) varies in the range of 2 - 16; both vertical jets and inclined jets without excess streamwise momentum are considered. The numerical results of the Standard two-equation k-ε model show that the turbulent structure can be broadly categorised according to the jet-to-crossflow velocity ratio. For strong to moderate jet discharges, i.e. R> 4, the jet is characterized by a longitudinal transition through a bent-over phase during which the jet becomes almost parallel with the main freestream, to a sectional vortex-pair flow with double concentration maxima; the computed flow details and scalar mixing characteristics can be described by self-similar relations beyond a dimensionless distance of around 20-60. The similarity coefficients are only weakly dependent on R. The cross-section scalar field is kidney-shaped and bifurcated, vvith distinct double concentr     

Regression Formulations for the Stress at Hot Spot of Multiplanar Tubular DT Joints

- Stress concentration analysis of multiplanar tubular DT joints plays an important role in the fatigue design of offshore platforms. A semi-analytic method for stress analysis under the condition of any loads is briefly introduced in the paper. Nineteen multiplanar tubular DT joints with one of two braces of the same dimension subjected to axial loads and out- of- plane bending moments are computed for parametric stress analysis by using the present method. The influence of geometrical parameters on the stresses of multiplanar tubular DT joints is discussed and compared with corresponding uniplanar T joints. The regression formulae for the stress at hot spot of multiplanar DT joints are found by modification of SCF of corresponding uniplanar T joints. The parametric formulae for the maximum stress by superposition. Finally, the influences of stiffening effect and load-interaction effect on the maximum stress of DT joints are discussed.