Expression of putative zinc-finger protein lcn61 gene in lymphocystis disease virus China （LCDV-cn） genome

An open reading frame (lcn61) of lymphocystis disease virus China (LCDV-cn), probably responsible for encoding putative zinc-finger proteins was amplified and inserted into pET24a (+) vector. Then it expressed in E. coli BL21 (DE3), and His-tag fusion protein of high yield was obtained. It was found that the fusion protein existed in E. coli mainly as inclusion bodies. The bioinformatics analysis indicates that LCN61 is C2H2 type zinc-finger protein containing four C2H2 zinc-finger motifs. This work provides a theory for functional research of lcn61 gene. Supported by High Technology Research and Development Program of China (863 Program, No. 2006AA100309)     

岩石变形破坏的熵突变过程与破坏判据

在岩石的变形破坏过程中,当进入不稳定的破裂发展阶段之后,系统不断调整结构抵抗外力的扰动,裂纹向局域集中的有序方向发展,应变能不断耗散,并以应变能的耗散为媒介,使系统与外扰动所追加的负熵流产生能量流通,也使系统熵值和系统的维数逐次降低,这一阶段岩石系统远离平衡态,岩石的破坏是系统熵的突变过程。基于这一认识,并在验证应变能分布与结构块度分布模式的一致性的基础上,推导出包含了结构因素和能量分布的熵表达式;对熵表达式进行平衡分析获得局部突变的分岔集,得到了岩石局部破坏的熵折迭突变破坏准则;同时,探讨了熵表达式所表征的结构有序度的尖点突变性,解出岩石系统的分岔集,这个分岔集就是岩石系统熵突变的整体破坏准则。     

Structure of the Potential Vorticity of an Explosive Cyclone over the Eastern Asian Region in Late November 2013

An explosive extratropical cyclone(EC)over the Eastern Asian region that caused two shipwrecks is analyzed using ERA-Interim reanalysis data from the European Center for Medium-Range Weather Forecasts.Analyses of the evolution of the EC reveal that the positive potential vorticity(PV)at the upper-tropospheric level displays a hook-shaped structure during the mature period of the cyclone.The PV distribution forms a vertically coherent PV structure called a PV tower.The vertical distribution of the PV can induce and strengthen cyclonic circulation from the lower-to upper-levels of troposphere,which is an important deepening mechanism of explosive cyclone.The PV tower occurs approximately ten hours prior to the development of surface occlusion in the cyclone.The evolution of surface fronts closely follows the development of the horizontal upper-tropospheric PV.This tandem development is largely attributed to the ability of the positive upper-tropospheric PV and the PV tower to induce cyclonic circulation simultaneously.The kinematic wrap-up process of cyclonic circulation also accelerates the formation of warm occlusion.A conceptual model of the distributions of positive PV and potential temperature combining the perspectives of dynamic tropopause folding,PV tower,and atmospheric stability,including westward tilting and baroclinicity,is proposed.This model can illustrate the explosive deepening mechanism of ECs.The regions of convective instability and rainfall determined by this model are consistent with those identified from the actual observation.     

地层证据--对大别造山带汇聚历史的制约

大别造山带的汇聚演化历史一直存在不同的认识,来自扬子陆块和华北陆块显生宙以来的地层记录以及北淮阳地区的地层记录可能为两个陆块的汇聚模式提供制约。通过对扬子陆块与华北陆块显生宙以来岩相古地理进行比较,其中3个阶段相似, 2个阶段差别很大。以浊积岩发育作为俯冲阶段的标志,磨拉石建造出现作为闭合的标志,显生宙以来,大别造山带曾经历了两次俯冲和两次汇聚,俯冲时间分别是晚奥陶世—早志留世和早三叠世,汇聚的时间分别为中 晚泥盆世和中三叠世,早期的汇聚具有软碰撞特征,以上认知得到了北淮阳地区地层、古生物和沉积记录的支持,同时也与大别山造山带榴辉岩的同位素年龄数据基本吻合。高压 超高压变质岩在晚三叠世处于快速折返阶段,早侏罗世以前到达地表,其平均折返隆升速率为39～66mm/a。     

基于生态足迹的区域生态适度人口研究——以新疆生产建设兵团为例

适度人口取决于生态、经济和土地等多要素的综合承载力。介绍了生态足迹模型的基本概念和计算方法,并运用该模型对兵团1995—2008年的生态足迹和生态承载力进行了计算,在此基础上对兵团的1995—2010年的生态适度人口进行了估算。结果表明,1995—1999年兵团的实际人口虽然在增加,但相对于生态适度人口并没有出现过剩;2000—2010年兵团生态适度人口在不断下降,实际人口和过剩人口却在不断增加。兵团目前已经出现了过度人口和生态赤字,人地矛盾相对紧张,生态适度人口规模是不合理的。     

鄂尔多斯盆地南部延长组富铀凝灰岩地球化学特征及形成机制

本文系统地研究了鄂尔多斯盆地南部延长组凝灰岩元素和同位素地球化学特征。结果表明：凝灰岩主量元素具有高K、高Al、Si中等至高、低Na的特征; REE总体特征为轻稀土富集、重稀土亏损,∑REE在4857～40212 μg/g 之间变化,Eu呈现出负异常,在034～082内变化,δCe范围在080～208之间; 微量元素中U含量在312～144 μg/g 之间,Ba、U、Th、Hf和Ce呈明显正异常,Nb、P、Ti和Rb呈现负异常。详细的分析表明：U的富集和一些微量元素如Ta、Dy、Lu等相关,Th与∑REE存在明显的正相关性,这些变化可能和盆地中铀矿的富集有关; 凝灰岩的硫同位素总体呈现深源特征。根据以上的研究对凝灰岩的形成原因及形成环境进行了探讨。鄂尔多斯盆地南部延长组凝灰岩来源于秦岭造山带火山作用,其中的铀元素富集是由于砂岩型铀矿中铀向凝灰岩迁移而被还原所致。本研究对盆地砂岩型铀矿的铀运移和富集机理认识具有一定指导意义。     

苏浙皖边界地区苏皖运动的有关问题

苏皖运动的存在于否,对研究苏浙皖边界地区的地质发展史和正确认识长兴阶地层有重要意义。笔者论证了长兴组的厚度变化、界线粘土层的性质与成因、底砾岩的确认和地层接触关系、古生物群的突变等问题,认为:1)区内苏皖运动是存在的;2)砺山-江阴海堤不可否定;将安徽广德牛头山经江苏宜兴至江阴条带内的龙潭组上部地层作为长兴组的相变产物是不妥的。     

柴达木盆地东部地区石炭系泥页岩生烃条件及选区

柴达木盆地石炭系露头、钻井资料均揭示,其为海相沉积烃源岩,且石炭系泥页岩气展现出良好的勘探前景。对柴达木盆地东部石炭系泥页岩沉积环境、有机质丰度、类型和演化程度进行综合评价,利用野外露头、探井、地震、非震等资料证实了石炭系的残留及分布。研究认为,柴东地区石炭系泥页岩主要集中在上石炭统滨岸潮坪沉积的克鲁克组,发育炭质泥页岩、暗色泥岩2种烃源岩,有机碳均值为3.08%,有机质类型为Ⅱ-Ⅲ型,处于成熟-高成熟演化阶段,73%的样品达到中等-好烃源岩。平面上主要分布于尕丘、欧南、霍布逊和德令哈凹陷,分隔性较强,具有多个厚度中心,其中以欧南凹陷石灰沟地区和霍布逊凹陷落实程度高、资源潜力大,是泥页岩气最有利的发育区。     

地球磁场对太阳风的加载响应与地震

地磁预报地震的方法越来越多,本文应用加卸载响应比方法,分析山西地区2009—2016年观测的加卸载响应比与山西地区4.0级地震之间的对应关系,初步给出适用于山西地区震例特征的磁异常判别指标。     

The Reference Manure Concept for Transformation Tests of Veterinary Medicines and Biocides in Liquid Manure

Veterinary medicines and biocides are frequently applied in animal houses of livestock husbandry. Due to these application patterns, they reach liquid manure. So far these substances are not transformed during manure storage, they enter soil by the application of manure as organic fertilizer. Therefore, biodegradability of veterinary medicines and biocides in manure is in the focus of regulatory procedures of environmental risk assessment. Since the representative and reproducible sampling of manures from high volume tanks is considered impossible due to high matrix variabilities, the reference manure concept was developed to utilize liquid bovine and pig manures for reproducible laboratory testing. Accordingly, excrement samples of cattle and pigs individually kept at experimental and conventional animal houses were taken. Tap water was added to matrix characterized excrements in order to prepare bovine and pig reference manures of 10 and 5% dry substance contents, respectively. Subsequently, the long‐term transformation of selected ¹⁴C‐labeled test substances was investigated under strictly anaerobic conditions. The application of different batches of bovine or pig reference manures indicated that extrapolation of transformation rates within the same animal species was possible. However, results cannot be transferred from bovine to pig manure because of substance specific interactions with the different manure matrices. The dependency of metabolic dynamics on different dry substance contents (2.5, 5, 10%) was additionally investigated in both manure matrices. These tests clearly showed that the dry substance content of the reference manure is one of the most relevant factors affecting the transformation of veterinary medicines and biocides. In contrast to highly variable tank manures, the reference manure concept allows for the exact adjustment of this parameter to guarantee reproducible laboratory testing.