高通量测序分析北仑河口红树林潮间带底栖真核生物分布

为了解红树林不同潮位沉积物中底栖真核生物群落分布,基于18S rRNA基因采用高通量测序方法分析了广西北仑河口陆缘、林中和海缘3个潮位红树林沉积物中底栖生物群落结构。结果表明,北仑河口潮间带红树林沉积物中底栖生物多样性丰富,Shannon-Wiener指数变化范围在6. 08～6. 73之间; PCA分析表明潮间带中底栖生物群落差异较大,陆缘红树林中扁形动物、节肢动物和软体动物相对丰度较高,林中区域中纤毛虫、环节动物和轮虫相对丰度较高,海缘红树林中硅藻相对丰度较高;红树林中主要OTUs有桡足类的太平洋纺锤水蚤(Acartia pacifica)、硅藻类的海链藻(Thalassiosira sp.)、纤毛虫类的前管虫(Prorodon teres)、多毛类的小头虫(Capitella sp.)。高通量测序方法能较全面反映红树林区微型/小型底栖生物群落,研究结果为丰富红树林底栖生物群落研究和解析底栖生物在红树林生态系统发挥的作用提供基础数据。     

用于海洋沉积物中厌氧氨氧化细菌分子生态学研究的引物比较

为比较厌氧氨氧化细菌16SrRNA基因的2对特异性引物(Amx368F/Amx820R、Brod541F/Amx820R)在其分子生态学研究方面的优点与不足,本研究采用高通量测序和qPCR技术对长江口及其邻近海域沉积物中厌氧氨氧化细菌细菌的群落组成、多样性和丰度进行比较分析,结果表明,引物Amx368F/Amx820R特异性较高,能够较为完整地反映沉积物中厌氧氨氧化细菌的多样性信息,且其覆盖的厌氧氨氧化细菌丰度与功能基因hzo丰度正相关(P<0.01);而引物Brod541F/Amx820R特异性较低,覆盖部分厌氧氨氧化细菌及其他多个门的细菌,其量化的厌氧氨氧化细菌与功能基因hzo丰度无明显相关关系(P>0.05)。因此,引物Amx368F/Amx820R能够更加真实地反映厌氧氨氧化细菌在海洋沉积物中的群落特征,在其分子生态学研究中更具优势。     

Spatio-temporal variability of small fishes related with environmental factors in a typical domestic tap water lake,Eastern China

The knowledge of prey small ?sh stock, distribution and abundance is necessary to guide stocking of piscivorous ?sh for the biomanipulation in domestic tap water lakes. This study describes the current status of small ?sh community in Kuilei Lake(China), and examines the spatial and seasonal variations of the community in relation to key environmental factors. Based on submerged macrophyte cover and water depth, the lake was divided into ?ve major habitats:(1) macrophyte covered shallow habitat of water depth< 2.00 m,(2) uncovered or less-covered shallow habitat(2.00 m–3.50 m),(3) uncovered medium shallow habitat(3.50 m–5.00 m),(4) uncovered medium deep habitat(5.00 m–6.50 m) and(5) uncovered deep habitat(6.50 m–8.50 m). The abundance and composition of small ?sh were monitored by benthic fykenet sampling from April 2013 to January 2014. A total of 2881 individuals belonging to 5 families and 21 species were collected. Based on their abundance(accounted for 88.96% of the total) and occurrence(more than 33.33%), Acheilognathus chankaensis, Acheilognathus macropterus, Microphysogobio microstomus,Pseudorasbora parva and Rhinogobius giurinus were recognized as dominant small ?sh species. The results of correlation analysis identi?ed that species richness( Sr), Shannon-Wiener diversity index( H′)and Margalef′s richness index( D) were signi?cantly negatively correlated with water depth, but positively correlated with biomass of submerged macrophytes.Redundancy analysis(RDA) revealed that the spatial distributions of most small ?shes were negatively associated with water depth. The details of these ?ndings are bene?cial to understanding the adaptation of the small ?shes in degraded environments, and to developing suitable biomanipulation strategies for the management of ?sh resources and water quality in the lakes along the lower reach of the Changjiang(Yangtze) River basin.     

Intraspecific morphometric diversity of barramundi (Lates calcariferBloch, 1790) in the waters of southern Bangladesh

A total of 160 barramundi's (Lates calcarifer Bloch, 1790) sampled from four rivers (Tentulia, Balaswar, Bakkhali, and Andarmanik) along the southern coastal region of Bangladesh were investigated in terms of morphometric characters to reveal the intraspecific variation. Twenty-five morphometric measurements were extracted using the conventional method and subjected to multivariate analyses (i.e., principal component analysis (PCA), discriminate function analysis (DFA), cluster analysis (CA)) to distinguish individuals from different rivers. The result demonstrated that twenty-two out of 25 measurements was statistically significant (Univariate ANOVA) among all four populations. PCA analysis of morphometric characters resulted in two principal components, PC I and PCⅡ which accounted for 79.25% and 4.28% of the total data variance. PC I-PC Ⅱ plot explained 83.53% of total variance differentiated the population of L. calcarifer into two groups. Discriminate analysis correctly classified about 88.1% of the examined fish into the four areas. The UPGMA dendrogram showed that Bakkhali populations were the most morphologically different populations in comparison to other populations, while Andarmanik and Balaswar populations were very close to each other. The strong morphometric variation between Bakkhali and Tentulia, Andarmanik and Balaswar was observed in the present study, suggested the evidence of the separate stock population of barramundi in these locations, which might require distinct stock management strategies for resource sustainability in the waters of southern Bangladesh. However, if these findings are supported by further molecular markers and geometric morphometry, this would be a strong indication of different stocks of this population in the four rivers of southern Bangladesh.     

面向城区宽基线立体像对视角变化的结构自适应特征点匹配

提出一种基于结构自适应特征的城区宽基线影像特征点匹配方法。首先,对影像提取点特征和直线特征,挖掘点特征与其邻域内直线特征之间的几何关系,构建结构自适应的特征区域和特征描述符,并通过双向匹配策略获得初始匹配结果。然后,基于初匹配结果估计影像基础矩阵,构建核线约束的结构自适应特征匹配算法进行二次匹配。最后,将已匹配特征作为控制基础设计匹配扩展算法,进一步增加匹配点数量。本文方法以特征点邻域几何结构为出发点,构建自适应的特征区域,能够在显著的影像视角变化下,为同名特征点提取影像内容一致的特征区域,进而获得相似的特征描述符。试验结果证明,与传统算法相比,本文方法在城区宽基线影像上能够同时获得更多的正确匹配特征和更高的匹配正确率。     

Search for H I emission from superdisk candidates associated with radio galaxies

Giant gaseous layers(termed “superdisks”) have been hypothesized in the past to account for the strip-like radio emission gap(or straight-edged central brightness depression) observed between twin radio lobes, in over a dozen relatively nearby powerful Fanaroff-Riley Class II radio galaxies. They could also provide a plausible alternative explanation for a range of observations. Although a number of explanations have been proposed for the origin of the superdisks, little is known about their material content. Some X-ray observations of superdisk candidates indicate the presence of hot gas, but a cool dusty medium also seems to be common. If they are entirely or partly composed of neutral gas, then it may be directly detectable and we report here a first attempt to detect/image any neutral hydrogen gas present in the superdisks that are inferred to be present in four nearby radio galaxies. We have not found a positive H I signal in any of the four sources, resulting in tight upper limits on the H I number density in the postulated superdisks,estimated directly from the central rms noise values of the final radio continuum subtracted image. The estimated ranges of the upper limit on neutral hydrogen number density and column density are 10^-4-10^-3 atoms per cm3 and 10^19-10^20 atoms per cm^2, respectively. No positive H I signal is detected even after combining all the four available H I images(with inverse variance weighting). This clearly rules out an H I dominated superdisk as a viable model to explain these structures, however, the possibility of a superdisk being composed of warm/hot gas still remains open.     

地基干涉雷达IBIS-S桥跨结构振动变形测量与模态分析

地基雷达干涉测量技术能够以较高的时空采样频率获取线性结构目标整体瞬时变形状态。本文将该技术引入大型桥梁桥跨结构的振动变形测量与模态分析中。对动静载试验过程中的武汉二七长江大桥东岸桥跨结构开展连续变形监测,利用频域分解法计算桥跨结构的自振频率、阻尼比和模态振型。计算结果表明,地基干涉雷达干涉测量技术能够快速探测桥跨结构竖弯模态,特别适用于灾后大型线形结构健康状态的应急检测。     

Archaeal community structure in sediments from a seamount in the Mariana Volcanic Arc

Seamounts are subsurface mountains in the ocean. Examination of the abundance and distribution of Archaea in seamount ecosystems may provide a better understanding of their ecological functions. Most studies of marine archaeal assemblages in seamount area have focused on hydrothermal vents or ferromanganese crusts. We investigated the archaeal communities from a seamount of the Mariana Volcanic Arc, in the tropical western Pacific Ocean by using high-throughput sequencing. Thaumarchaeota was dominant in the sediments of all sample stations. Community diversity and species richness were greatest at stations near the top of the seamount, and lowest at the deepest station. One sample station on the steep southeast slope that faced the Yap-Mariana trench had a unique composition of Archaea. In summary, depth has an important influence on archaeal community structure, and the geographic properties and sediment characteristics may explain the unique distribution patterns of Archaea in this seamount. This study provides a foundation for future research on Archaea in seamounts.     

多尺度邻域特征下的机载LiDAR点云电力线分类

利用机载激光雷达技术三维测量精度高且获取快速的优点进行电力线自动分类提取已成为点云数据处理与电力应用的重要领域。针对电力线分类模型的自动化和高精度需求,本文提出了基于三维多尺度邻域特征的机载LiDAR点云电力线分类提取模型框架,主要包括4个步骤：电力线候选点滤波、多尺度邻域类型选取、形状结构特征提取和支持向量机分类。通过对2个复杂城市区域的试验数据集和8种不同邻域类型的详细结果对比分析,发现基于多尺度圆球邻域形状结构特征的分类模型结果准确率、召回率和质量分别达到97%、94%和93%,同时整体处理时间在2个试验数据中分别从366、256 s减少到274、160 s。试验结果表明,该方法在多种复杂城市场景中能够实现机载LiDAR数据的电力线较高精度分类提取。     

GF-3全极化影像在地表浅覆盖区进行地质构造解译的新方法

地质构造信息对地质矿产调查具有重要意义,野外实测和光学遥感等常规手段在一些地表浅覆盖区获取的地质构造信息十分有限,而合成孔径雷达(SAR)对地表具有一定的穿透性,在探测地表浅层覆盖区域的地质构造特征中具有独特优势。利用高分三号(GF-3)全极化影像,在典型的地表浅层覆盖区域,开展了断裂构造等信息的解译探索,提出了一种地表浅覆盖区域地质构造解译的新方法。首先对西藏改则、林芝、贵阳、北京千家店等4个研究区内的断裂构造和环带构造进行分析;接着,提出了GF-3全极化影像用于浅覆盖区地质解译的处理流程,通过引入DEM数据对GF-3影像进行地形校正,充分利用微地形微地貌特征,并采用不同极化方式的RGB合成,增强了影像的判读性,并进行地质构造解译;最后,将解译结果与1∶5万实测数据进行对比,断层的位置和方向与实测结果基本一致,同时获取了大量野外实测未能探明的浅覆盖层以下的断层信息,进一步丰富了研究区的地质构造信息。结果表明,GF-3全极化影像可用于浅覆盖区的地质构造解译,并且具有野外实测和光学遥感等常规手段所不能替代的独特优势。