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411.
Non-specific immune response of bullfrog Rana catesbeiana to pathogenic Aeromonas hydrophila was studied to 60 individuals in two groups. Each bullfrog in bacterium-injected group was injected intraperitoneally (i.p.) with 0.2 ml bacterial suspension at a density of 5.2 × 10^6 CFU/ml, while each one in control group injected i.p. with 0.2 ml sterile saline solution (0.85%, w/v). Three bullfrogs in both groups were sampled at 0, 1, 3, 7, 11, 15 and 20 days post-injection (dpi) for the evaluation of non-specific immune parameters. It was observed that intraperitoneal injection of A. hydrophila significantly increased the number of leucocytes and that of NBT-positive cells in peripheral blood. Significant increases in serum bactericidal activity and serum acid phosphatase activity were also observed in the bacterium-injected frogs when compared with those in the control group. However, a significant reduction was detected in vitro in phagocytosis activity of peripheral blood phagocytes. No significant difference in changes in the number of peripheral erythrocytes, serum superoxide dismutase (SOD) activity, and lysozyme activity was detected between the two groups. It is suggested that bullfrogs may produce a series of non-specific immune reactions in response to the A. hydrophila infection. 相似文献
412.
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416.
以海鞘(Ciona savignyi)为研究对象,考察了海鞘血细胞原代培养方法及培养中细菌污染的鉴定和控制.血细胞在培养1 h后贴壁,细胞基本以圆形细胞为主,变形细胞一般呈不规则形.变形细胞存活时间较短,而圆形细胞存活时间较长.对于培养过程中的细菌污染,通过细菌分离、培养和纯培养发现两类菌株检出率较高,均为革兰氏阴性菌.经PCR扩增16S rDNA基因序列片断,结果显示这两类菌株分别属于弧菌属(Vibrio)和施万氏菌属(Shewanella).药敏试验结果显示,弧菌属对氯霉素和环丙沙星等具有较强的敏感性;对施万氏菌属较敏感的药物依次为亚胺培南和氯霉素等.最后比较了几种抗生素组合控制血细胞培养中细菌污染的使用效果,其中氯霉素、亚胺培南加双抗的组合有较好的抑菌效果并对培养细胞的贴壁和生长没有影响. 相似文献
417.
盐水闪蒸技术广泛应用于海水淡化及海洋温差能发电,含盐质量分数、给盐液温度和闪蒸压力对闪蒸汽化率和热效率有很大影响。在质量分数为3.5%~20%、给盐液温度为30~95℃和闪蒸压力为1~30 kPa的范围内,对NaCl溶液闪蒸汽化率和热效率等闪蒸效率特性进行了定量计算研究,研究得到了盐水含盐质量分数、给盐液温度和闪蒸压力对闪蒸汽化率以及热效率影响的规律。结果表明,降低含盐质量分数,提高给盐液温度,降低闪蒸压力可提高闪蒸汽化率与热效率;含盐质量分数越高,造成的热损失越大,而提高给盐液温度会增大热损失。 相似文献
418.
In this paper,the analysis method of stochastic response of piled offshore platform excited bystationary filtered white noise is presented.With this method,the strong ground motion is considered asthree direction stationary filtered white noise process,the theoretic solutions of three special integrationequations are derived with the residue theorem,and the expression of response nodal displacements andmember forces of offshore platform excited by the stationary filtered white noise is put forward.Thestochastic response of a piled offshore platform excited by the stationary filtered white noise,which is lo-cated 114.3m in water depth,is computed.The results are compared with those obtained with the re-sponse spectrum analysis method and the stationary white noise model analysis method,and the corre-sponding conclusion is drawn. 相似文献
419.
魁蚶(Scapharca broughtonii)半乳糖凝集素(SbGal)基因cDNA的克隆及表达分析 总被引:1,自引:2,他引:1
半乳糖凝集素(Galectin)作为动物凝集素家族的一员,是一种重要的免疫因子,在脊椎动物和无脊椎动物中都参与了重要的免疫防御反应。为明确魁蚶(Scapharca broughtonii)半乳糖凝集素基因的结构特征、组织分布及其对病原菌刺激的免疫反应规律,本研究从构建的魁蚶转录组文库中筛选得到半乳糖凝集素基因部分序列,通过c DNA末端快速扩增(rapid amplication of c DNA ends,RACE)技术获得该基因c DNA全长序列(命名为Sb Gal)。序列和结构分析表明,该c DNA全长3120bp,编码554个氨基酸,是一个含有4个糖识别结构域(CRD)的且在双壳贝类中较为独特的半乳糖凝集素;预测蛋白的分子量(Mw)为63.3158 k Da,理论等电点(p I)为4.99。同源性及系统分析表明,Sb Gal基因与美洲牡蛎、合浦珠母贝、海湾扇贝galectin的同源性分别为70%、65%、46%,Sb Gal基因四个CRD彼此的相似性为36%—41%。实时荧光定量PCR(q RT-PCR)结果表明:Sb Gal m RNA在魁蚶血细胞、斧足、闭壳肌、外套膜、鳃和肝胰腺中均有表达,血细胞中的表达量最高,在肝胰腺中的表达量最低;注射鳗弧菌后,相对于对照组,Sb Gal基因在所检测的每个组织中m RNA水平上的表达量都显著上调(P0.01),而且表达量具有显著的时间依赖性和瞬时表达趋势,呈现先升高后降低的趋势。 相似文献
420.
Helical piles are structural deep foundation elements, which can be categorized as torque-driven piles without any limitations to implement in marine situations. Different methods are used to predict the axial capacity of helical piles, such as static analysis, but have some limitation for this type of piles on marine conditions. In situ testing methods as supplement of static analysis have been rarely used for helical piles. In geotechnical engineering practice, the most common in situ tests particularly applicable for coastal or offshore site investigation are cone penetration test (CPT) and piezocone penetration test (CPTu). The CPT is simple, repeatable, and prepares the continuous records of soil layers. In this paper, a data bank has been compiled by collecting the results of static pile load tests on thirty-seven helical piles in ten different sites including CPT or CPTu data. Axial capacities of thirty-seven helical piles in different sites were predicted by direct CPT methods and static analysis. Accuracy estimation of ten direct CPT methods to predict the axial capacity of helical piles was investigated in this study. Comparisons have been made among predicted values and measured capacity from the pile load tests. Results indicated that the recently developed methods such as NGI-05 (2005), ICP-05 (2005), and UWA-05 (2005) predicted axial capacity of helical piles more accurately than the other methods such as Meyerhof (1983), Schmertmann (1978), Dutch (1979), LCPC (1982), or Unicone (1997). However, more investigations are required to establish better correlation between CPT data and axial capacity of helical piles. 相似文献