固定化鲅鱼乙酰胆碱酯酶的制备及部分性质测定

酶的固定化是酶传感器制备过程中重要的1个环节.研究采用直接共价法固定鲅鱼乙酰胆碱酯酶（AChE）.制备方法为：0.1g CNBr活化的Sepharose 4B凝胶用1mmol/L的HCl充分溶胀后,与活力为10U的AChE溶液混合,于4℃下150r/min振荡8h.所制备的固定化酶活力回收率较高（96%）,对pH值和温度变化的适应能力均优于非固定化酶;在3个月保存期内,前者的活力损失13%,而后者的活力则下降89%.这说明固定过程能够大大提高AChE的抗逆性和保持酶活力的稳定,有利于酶传感器的制备.     

溴素的有机化学──Ⅴ.溴代脂肪酸—2,3,5,6—四溴—1,4—苯二酯的合成

以1，4—苯二酚和不同的短链脂肪酸为基本原料，经溴代、加成、形成酰卤和酰化等反应，合成了六种新的溴化脂肪酸—2，3，5，6—四溴—1，4—苯二酯。通过对产物的碳、氢、溴等元素的定量分析，以及红外吸收光谱和氢核磁共振谱的研究，验证了化合物的结构。     

正压浅海湍流运动的雷诺应力封闭模型

根据湍流封闭理论,建立一种适用于正压浅海湍流运动的雷诺应力封闭模型(RSM),以代替目前三维浅海动力学模型中普遍采用的湍粘性系数的传统假设。通过直接建立并模化f—平面上正压海洋的雷诺应力传输方程,分别得到的微分形式和代数形式的RSM方程组。并讨论了进行数值计算所需要的边界条件。利用该模型可以进一步研究浅海潮流、风暴潮流及风海流等浅海流动的三维结构和湍流特性。     

Numerical Study on Instantaneous Discharge of Unsorted Particle Cloud in Cross Flow

The mixing characteristics of particles such as dredged sediment of variable size discharged into cross flow are studied by a 3D numerical model, which is developed to model the particle-fluid two-phase flow. The Eulerian method with the modified k-ε parameterization of turbulence for the fluid phase is used to solve fluid phase, while a Lagrangian method for the solid phase (particles), both the processes are coupled through the momentum sources. In the model the wake turbulence induced by particles has been included as additional source term in the k-ε model; and the variable drift velocities of the particles are treated efficiently by the Lagrangian method in which the particles are tracked explicitly and the diffusion process is approximated by a random walk model. The hydrodynamic behavior of dumping a cloud of particles is governed by the total buoyancy of the cloud, the drag force on each particle and the velocity of cross-flow. The computed results show a roughly linear relationship between the displacement of the frontal position and the longitudinal width of the particle cloud. The particle size in the cloud and the velocity of cross flow dominate the flow behavior. The computed results are compared with the results of laboratory experiments and satisfactory agreement is obtained.     

南海及周边部分地区特提斯构造遗迹:问题与思考

对全球二叠纪－三叠纪古气候特征的研究表明，东特提斯地区晚三叠世古气候标志具有带状分布的特征。北巴拉望地块卡拉棉群岛科龙岛的晚三叠世沉积中发育反映较干燥古气候特征的鲕状灰岩，因此在古气候特征方面，北巴拉望地块晚三叠世地层不能与华南和印支地块同期地层对比，而应该与印度地块北缘喜马拉雅地区（向西）和琉球群岛（向东）对比。根据该古气候分析，北巴拉望地块在晚三叠世时应该位于南部亚热带较干燥气候带内，其晚三叠世的古地理位置比印支地块更靠南。对南海及南海周边部分地特提斯构造遗迹的研究，将有助于深入了解南海北部陆缘区相关构造带的性质及正确恢复南海扩张之前该区的地质发展历史。南海北部陆缘区也是研究解决特提斯构造向东延伸问题以及特提斯构造－太平洋构造时空转化问题的关键地区。     

东营城区高精度三维地震采集方法研究

根据东营城区内建筑物较多，环境干扰较大，地下构造复杂，断裂非常发育，目的层埋藏深，资料信噪比较低的特点，以地震老资料和钻井资料为基础，建立地震地质模型，利用射线追踪技术选取合适的观测系统参数。充分利用卫星数字地图，没计了灵活多变的观测系统，采用可控震源与炸药震源配合使用方法，及时对城区内地下面元的覆盖次数、方位角和炮检距的分布进行质量监控，利用现场处理系统对资料进行及时分析，提高了地震资料品质。所获得的东营城区高精度三维地震采集资料显示，其浅中层分辨率和中深层信噪比都有了明显改善，取得了良好的地质效果。     

桶形基础平台三维有限元稳定性分析

以我国第一座桶形基础采油平台工程实例为原型，采用三维有限元计算方法对桶形基础稳定性分析。平台在波浪、风等荷载作用下，其稳定性不仅与平台的整体结构有关，而且与桶形基础及其周围土体相互作用相关。在计算中，利用薄层摩擦单元模拟土与结构间的相互作用，取得了很好的效果。分析结果表明桶形基础采油平台在设计条件下运行是较为稳定的。     

Sediment–water interactions of natural oestrogens under estuarine conditions

The natural human female hormones oestrone and 17β-oestradiol have been implicated in the disruption of endocrine systems in some wildlife adjacent to sewage effluents. The sorption behaviour of these two compounds under estuarine conditions was studied by spiking either 2.55 μg of oestrone or 2.65 μg of 17β-oestradiol in kinetic experiments. In equilibrium experiments, 3 ng of oestrone or 3.2 ng of 17β-oestradiol was added in each of the centrifuge tubes. Sorption onto sediment particles was relatively slow, with sorption equilibrium being reached in about 70 and 170 h for oestrone and 17β-oestradiol, respectively. The effects of a variety of environmental parameters on sorption were studied including salinity, sediment concentration (SC), the presence of a third phase, particle size and, also, surfactant concentrations. Results show that although salinity did not induce any statistically significant effect on the sorption of 17β-oestradiol, it did statistically enhance the sorption of oestrone, and a salting constant of 0.3 l mol⁻¹ was derived. The partition coefficient for both compounds decreased with increasing sediment concentration, a phenomenon that has been widely reported and attributed to the presence of colloids (which could enhance dissolved concentrations). In this paper, the true partition coefficients for sediment particles (K_p^true) and colloidal particles (K_c^true) have been calculated, and a K_p^true value of 141 and 102 ml g⁻¹ was obtained for oestrone and 17β-oestradiol, respectively. In addition, K_c^true values for oestrone (222×10² ml g⁻¹) and 17β-oestradiol (135×10² ml g⁻¹) were two orders of magnitude higher than their respective K_p^true values, suggesting that the colloidal particles are significantly stronger sorbents for natural oestrogens than sediment particles. Particles of different sizes were found to have different partition coefficients due to the strong relationships between partition coefficients for the two compounds and particulate organic carbon (POC) contents and specific surface areas (SSAs). The presence of a surfactant was shown to reduce the partition coefficients for the two compounds, although its concentrations being used were higher than those normally found in the natural environment.     

添加维生素B_（12）生产菌高密度培养褶皱臂尾轮虫的效果

阐述把维生素Ｂ１２生产菌添加到培养水中培养褶皱臂尾轮虫ＢｒａｃｈｉｏｎｕｓＰｌｉｃａｔｉｌｉｓ的买验。共１８株细菌分离于轮虫培养池，其中，有一株产维生素Ｂ１２的假单胞杆菌ＴＰ４对轮虫的生长繁殖有明显的促进作用。把ＴＰ４菌株培养后，加入到２Ｌ的烧杯和５００Ｌ的水槽中培养泰国Ｓ型轮虫时．在９ｄ（天）和６ｄ（天）中，轮虫密度从１２４～１３９和２４２～２８８个体／ｍｌ增殖到４，４１７～５，５４０和１，０１７～１，２５４个体／ｍｌ，分别比对照组增加了４～６及２～３倍。     

崂山花岗岩地区含锶、偏硅酸矿泉水的形成机理

报道了崂山花岗岩地区矿泉水的特征。指出其矿泉类型为含锶、偏硅酸型，并讨论了其形成机理。