微小卡罗藻溶血活性的生长期特征及其溶血毒素种类分析

研究微小卡罗藻的生长曲线及不同生长期藻细胞提取液和去藻上清液溶血活性的大小,通过乙酸乙酯和Bligh-Dyer两种方法提取溶血毒素,利用柱层析和薄层层析对溶血毒素成分进行初步的分离与分析。结果表明,藻细胞提取液在4个生长阶段中都具有明显的溶血活性,去藻上清液在平稳期和衰亡期具有明显的溶血活性,延滞期及对数生长期溶血活性很低,表明微小卡罗藻的溶血物质是其自身正常生理代谢产物,且在细胞老化过程中可以释放至水体环境中。两种提取方法都可以将溶血毒素提取出来,进一步的色谱分离和生物活性检测发现微小卡罗藻主要含有3种不同性质的溶血毒素,其溶血作用可能与微小卡罗藻较高含量的多不饱和脂肪酸18:5n3和22:6n3有关。     

1998年季风爆发期南海大气边界层的日变化

在南海尚未有关于海洋大气边界层结构日变化方面的研究.研究海洋大气边界层,有利于我们更好地研究海洋表层结构变化影响机制,对发生在海洋大气边界层上的过程进行预测预报.因此了解南海海洋大气边界层的结构特征,对于我们进一步了解南海天气尺度海气相互作用有非常重要的意义.通过对1998年在南海南部(6°15′N,110°E)和南海北部(20°29′39″N,116°57′48″E)的南海季风试验中定点科考船释放一天四次的探空观测资料分析得出,季风爆发前海洋大气边界层存在规则的日变化,在中午达到深厚.季风爆发后南海北部大气边界层日变化消失,南海南部依然明显.分析表明对其日变化起重要影响的是短波辐射;潜热输送对大气边界层高度日变化影响不大.与大气边界层厚度日变化相对应,南海南部边界层内水汽日变化明显,而南海北部较不明显.     

Characterization of diurnal cycles in ZTD from a decade of global GPS observations

The diurnal cycle of the tropospheric zenith total delay (ZTD) is one of the most obvious signals for the various physical processes relating to climate change on a short time scale. However, the observation of such ZTD oscillations on a global scale with traditional techniques (e.g. radiosondes) is restricted due to limitations in spatial and temporal resolution. Nowadays, the International GNSS Service (IGS) provides an important data source for investigating the diurnal and semidiurnal cycles of ZTD and related climatic signals. In this paper, 10 years of ZTD data from 1997 to 2007 with a 2-hour temporal resolution are derived from global positioning system (GPS) observations taken at 151 globally distributed IGS reference stations. These time series are used to investigate diurnal and semidiurnal oscillations. Significant diurnal and semidiurnal oscillations of ZTD are found for all GPS stations used in this study. The diurnal cycles (24 hours period) have amplitudes between 0.2 and 10.9 mm with an uncertainty of about 0.5 mm and the semidiurnal cycles (12 h period) have amplitudes between 0.1 and 4.3 mm with an uncertainty of about 0.2 mm. The larger amplitudes of the diurnal and semidiurnal ZTD cycles are observed in the low-latitude equatorial areas. The peak times of the diurnal cycles spread over the whole day, while the peak value of the semidiurnal cycles occurs typically about local noon. These GPS-derived diurnal and semidiurnal ZTD signals are similar with the surface pressure tides derived from surface synoptic pressure observations, indicating that atmospheric tides are the main driver of the diurnal and semidiurnal ZTD variations.     

Preindustrial, historical, and fertilization simulations using a global ocean carbon model with new parameterizations of iron limitation, calcification, and N2 fixation

The Canadian Model of Ocean Carbon (CMOC) has been developed as part of a global coupled climate carbon model. In a stand-alone integration to preindustrial equilibrium, the model ecosystem and global ocean carbon cycle are in general agreement with estimates based on observations. CMOC reproduces global mean estimates and spatial distributions of various indicators of the strength of the biological pump; the spatial distribution of the air-sea exchange of CO₂ is consistent with present-day estimates. Agreement with the observed distribution of alkalinity is good, consistent with recent estimates of the mean rain ratio that are lower than historic estimates, and with calcification occurring primarily in the lower latitudes. With anthropogenic emissions and climate forcing from a 1850-2000 climate model simulation, anthropogenic CO₂ accumulates at a similar rate and with a similar spatial distribution as estimated from observations. A hypothetical scenario for complete elimination of iron limitation generates maximal rates of uptake of atmospheric CO₂ of less than 1 PgC y⁻¹, or about 11% of 2004 industrial emissions. Even a ‘perfect’ future of sustained fertilization would have a minor impact on atmospheric CO₂ growth. In the long term, the onset of fertilization causes the ocean to take up an additional 77 PgC after several thousand years, compared with about 84 PgC thought to have occurred during the transition into the last glacial maximum due to iron fertilization associated with increased dust deposition.     

Variations in the quantity and composition of seston from an estuary in southern Chile on different temporal scales

Estuaries are characterised by highly variable environmental conditions largely driven by tidal and atmospheric forces. This study investigates variation in the physical environment and the composition of the seston on various temporal scales in the Quempillén estuary, southern Chile. The water column was sampled throughout the tidal cycle at various times of the year. Total particulate matter, particulate inorganic matter, particulate organic matter, particle numbers, total particle volume, proximate biochemical composition and energy content of the seston, chlorophyll a and chloropigments were routinely measured. In each of the months in which sampling took place, two or three tidal cycles were examined. The information not only helps to explain the dynamics of the estuary, but is essential for an understanding of the physiology and ecology of the suspension-feeders which exploit the seston as a food source, the most dominant being the gastropod Crepipatella dilatata. Temperature and salinity were generally highest during summer, but seston quality, defined by energy content and biochemical composition (lipid, protein and carbohydrate) was higher at the end of winter and during spring. Chlorophyll a values were greatest in late spring (November). Many of the variables studied changed frequently according to the phase of the tidal cycle, and in several cases significant differences were observed among tidal cycles from the same month of the same year. In general the variables measured did not exhibit consistent patterns linked to the tidal cycle, possibly because any such patterns were masked by atmospheric conditions (wind and rain) that dominate the region and greatly influence the estuary. The quantity and quality of the seston available to suspension-feeders is largely determined by these atmospheric forces, which cause an influx of terrigenous material from adjacent areas and also resuspend bottom sediment. These effects are magnified by the shallowness of the estuary (<2 m depth). The food supply for C. dilatata and other suspension-feeders therefore varies on temporal scales varying from hourly (tidal cycle) to daily/weekly (atmospheric forces) to monthly (seasonal influences), but inhibition of feeding by low salinity sometimes limits the ability of C. dilatata to exploit fully the available organic matter.     

Modelling and mass balance assessments of nutrient retention in a seasonally-flowing estuary (Swan River Estuary,Western Australia)

An integrated mass balance and modelling approach for analysis of estuarine nutrient fluxes is demonstrated in the Swan River Estuary, a microtidal system with strong hydrological dependence on seasonal river inflows. Mass balance components included estimation of gauged and ungauged inputs to the estuary and losses to the ocean (outflow and tidal exchange). Modelling components included estimation of atmospheric (N fixation, denitrification) and sediment–water column nutrient exchanges. Gross and net denitrification derived using two independent methods were significantly correlated (r² = 0.49, p < 0.01) with net rates averaging 40% of gross. Annual nitrogen (N) and phosphorus (P) loads from major tributaries were linearly correlated with annual freshwater discharge and were 3-fold higher in wet years than in dry years. Urban drains and groundwater contributed, on average, 26% of N inputs and 19% of P inputs, with higher relative contributions in years of low river discharge. Overall, ungauged inputs accounted for almost 35% of total nitrogen loads. For N, elevated loading in wet years was accompanied by large increases in outflow (7x) and tidal flushing (2x) losses and resulted in overall lower retention efficiency (31%) relative to dry years (70%). For P, tidal flushing losses were similar in wet and dry years, while outflow losses (4-fold higher) were comparable in magnitude to increases in loading. As a result, P retention within the estuary was not substantially affected by inter-annual variation in water and P loading (ca. 50% in all years). Sediment nutrient stores increased in most years (remineralisation efficiency ca. 50%), but sediment nutrient releases were significant and in some circumstances were a net source of nutrients to the water column.     

Nutrient flux into an intense deep chlorophyll layer in a mode-water eddy

An intense deep chlorophyll layer in the Sargasso Sea was reported near the center of an anticyclonic mode-water eddy by McGillicuddy et al. [2007. Eddy–wind interactions stimulate extraordinary mid-ocean plankton blooms, Science, accepted]. The high chlorophyll was associated with anomalously high concentrations of diatoms and with a maximum in the vertical profile of ¹⁴C primary productivity. Here we report tracer measurements of the vertical advection and turbulent diffusion of deep-water nutrients into this chlorophyll layer. Tracer released in the chlorophyll layer revealed upward motion relative to isopycnal surfaces of about 0.4 m/d, due to solar heating and mixing. The density surfaces themselves shoaled by about 0.1 m/d. The upward flux of dissolved inorganic nitrogen, averaged over 36 days, was approximately 0.6 mmol/m²/d due to both upwelling and mixing. This flux is about 40% of the basin wide, annually averaged, nitrogen flux required to drive the annual new production in the Sargasso Sea, estimated from the oxygen cycle in the euphotic zone, the oxygen demand below the euphotic zone, and from the ³He excess in the mixed layer. The observed upwelling of the fluid was consistent with theoretical models [Dewar, W.K., Flierl, G.R., 1987. Some effects of wind on rings. Journal of Physical Oceanography 17, 1653–1667; Martin, A.P., Richards, K.J., 2001. Mechanisms for vertical nutrient transport within a North Atlantic mesoscale eddy. Deep-Sea Research II 48, 757–773] in which eddy surface currents cause spatial variations in surface stress. The diapycnal diffusivity at the base of the euphotic zone was 3.5±0.5×10⁻⁵ m²/s. Diapycnal mixing was probably enhanced over more typical values by the series of storms passing over the eddy during the experiment and may have been enhanced further by the trapping of near-inertial waves generated within the eddy.     

Dimethylsulfide production in Sargasso Sea eddies

Lagrangian time series of dimethylsulfide (DMS) concentrations from a cyclonic and an anticyclonic eddy in the Sargasso Sea were used in conjunction with measured DMS loss rates and a model of vertical mixing to estimate gross DMS production in the upper 60 m during summer 2004. Loss terms included biological consumption, photolysis, and ventilation to the atmosphere. The time- and depth (0–60 m)-averaged gross DMS production was estimated to be 0.73±0.09 nM d⁻¹ in the cyclonic eddy and 0.90±0.15 nM d⁻¹ in the anticyclonic eddy, with respective DMS replacement times of 5±1 and 6±1 d. The higher estimated rate of gross production and lower measured loss rate constants in the anticyclonic eddy were equally responsible for this eddy's 50% higher DMS inventory (0–60 m). When normalized to chlorophyll and total dimethylsulfoniopropionate (DMSP), estimated gross production in the anticyclonic eddy was about twice that in the cyclonic eddy, consistent with the greater fraction of phytoplankton that were DMSP producers in the anticyclonic eddy. Higher rates of gross production were estimated below the mixed layer, contributing to the subsurface DMS maximum found in both eddies. In both eddies, gas exchange, microbial consumption, and photolysis were roughly equal DMS loss terms in the surface mixed layer (0.2–0.4 nM d⁻¹). Vertical mixing was a substantial source of DMS to the surface mixed layer in both eddies (0.2–0.3 nM d⁻¹) owing to the relatively high DMS concentrations below the mixed layer. Estimated net biological DMS production rates (gross production minus microbial consumption) in the mixed layer were substantially lower (by almost a factor of 3) than those estimated in a previous study of the Sargasso Sea, which may explain the relatively low mixed-layer DMS concentrations found here during July 2004 (3 nM) compared to previous summers (4–6 nM).     

热带太平洋次表层三维海温距平场的EOF分析

应用太平洋次表层海温距平资料构造了一个立体的西低东高的四级阶梯模型,使用EOF方法对此模型进行时空分解,重点讨论了分解结果与ENSO循环的关系,并对Nino 3距平指数进行试预报,结果表明,用 EOF分解的第一主分量代表暖池-厄尔尼诺(拉尼娜)模态,第二主分量代表次表层温度距平的东西运移模态,厄尔尼诺事件正是东西运移模态突变的结果.时滞相关分析估算,一次ENSO循环的平均周期约为41个月,厄尔尼诺、拉尼娜模态与温度距平的东西运移模态的位相差平均约为9.7个月.应用逐步回归方法得到超前Nino 3距平指数3、6和12个月的3个预报方程.预报结果表明,第一、第二时间系数对Nino 3距平指数均具有一定的预报价值,预报时效可达1 a左右.     

Benthic nitrate biogeochemistry affected by tidal modulation of Submarine Groundwater Discharge (SGD) through a sandy beach face, Ria Formosa, Southwestern Iberia

To investigate both the role of tides on the timing and magnitude of Submarine Groundwater Discharge (SGD), and the effect on benthic nitrogen biogeochemistry of nitrate-enriched brackish water percolating upwards at the seepage face, we conducted a study of SGD rates measured simultaneously with seepage meters and mini-piezometers, combined with sets (n = 39) of high resolution in-situ porewater profiles describing NH₄⁺, NO₃⁻, Si(OH)₄ and salinity distribution with depth (0–20 cm). Sampling took place during two consecutive spring tidal cycles in four different months (November 2005, March, April and August 2006) at a backbarrier beach face in the Ria Formosa lagoon, southern Portugal. Our results show that the tide is one of the major agents controlling the timing and magnitude of SGD into the Ria Formosa. Intermittent pumping of brackish, nitrate-bearing water at the beach face through surface sediments changed both the magnitudes and depth distributions of porewater NH₄⁺ and NO₃⁻ concentrations. The most significant changes in nitrate and ammonium concentrations were observed in near-surface sediment horizons coinciding with increased fraction of N in benthic organic matter, as shown by the organic C:N ratio. On the basis of mass balance calculations executed on available benthic profiles, providing ratios of net Ammonium Production Rate (APR) to Nitrate Reduction Rate (NRR), coupled to stoichiometric calculations based on the composition of organic matter, potential pathways of nitrogen transformation were speculated upon. Although the seepage face occasionally contributes to reduce the groundwater-borne DIN loading of the lagoon, mass balance analysis suggests that a relatively high proportion of the SGD-borne nitrogen flowing into the lagoon may be enhanced by nitrification at the shallow (1–3 cm) subsurface and modulated by dissimilatory nitrate reduction to ammonium (DNRA).