宁波沿海陆源排污口放线菌(Actinomycetales sp.)的分布特点

利用454高通量测序技术对宁波沿海10个陆源排污口20个站位的放线菌的时空分布及5个工业排污口的放线菌的种类作了整体分析。成功鉴定出了83个属,84个种。研究结果显示:放线菌在陆源排污口的分布呈现季节性分布,从3月份到10月份,放线菌数量呈现先升高后下降的变化,5月份和8月份数量居高,在3月份和10月份偏低;在综合排污口(S4,S6,S8和S9)检测频次较高,在工业排污口(S1,S3,S5,S7和S10)检测频次居中,在市政排污口(S2)检出频次最低。不同类型的排污口,氨氮浓度的排出量不同,放线菌的种类和数量也不同。在5个工业排污口中,S7和S10检出的共同菌最多;在S7独自检出短双歧杆菌(Bifidobacterium breve)、长双歧杆菌(B.longum)和两岐双岐杆菌(B.bifidum),表明存在粪源污染物;在S5检出皱孢链霉菌(Streptomyces scabrisporus)和硫藤黄链霉菌(S.thioluteus),显示有石油降解物和重金属的污染。总体上看,放线菌数量在距排污口外50m处略高于排污口处。排放指标越相似,菌的种类越接近。     

基于第二代测序技术的条斑紫菜(Pyropia yezoensis)贝壳丝状体附生菌群研究

条斑紫菜(Pyropia yezoensis)贝壳丝状体黄斑病给育苗产业造成危害,但目前对健康状态下的菌群结构及其发病后变化仍缺乏认识。本研究基于第二代测序技术和16S r RNA序列分析,对健康和患病丝状体的细菌组成与变化进行比较。测序共产生1.6Gb配对末端序列,获得了第一个高通量的条斑紫菜贝壳丝状体细菌群落数据集。通过数据分析共识别出7,833种可操作分类单元(operational taxonomic units,OTUs),比对确定为18个细菌门。相对于健康样本,黄斑病丝状体样本的微生物多样性显著增加。主坐标分析和非加权组平均法(unweighted pair group method using arithmetic average,UPGMA)聚类均将健康和黄斑病丝状体分成不同的组,且健康组样本间的距离明显小于黄斑病组。上述结果说明,健康贝壳丝状体保持着相似的微生物群落结构,而患病后的丝状体则发生了不同的变化;健康样本和黄斑病样本组间差异显著的细菌属有39个,其中29个在健康样本中的相对丰度明显高于病患组样本。本研究从菌群生态角度分析了健康贝壳丝状体微生物菌群结构、发病后菌群的变化及其对丝状体生长的潜在作用,为丝状体病害的检测和预防提供有价值的信息。     

白缘(鱼央)5S rDNA的扩增分析及其在染色体上的FISH定位

本文分别对雌雄白缘(鱼央)的5S rDNA进行了PCR扩增和序列分析,并采用双色荧光原位杂交(FISH)技术,以白缘(鱼央)的5S rDNA序列和小麦的45S rDNA为探针,对其在白缘(鱼央)雌雄中期染色体上进行了FISH定位研究。结果表明,白缘(鱼央)5S rDNA序列无雌雄差异;5S rDNA的保守区序列为117 bp;5S rDNA和45S rDNA分别被定位于白缘(鱼央)的性染色体和第5号染色体上。同时从GenBank中获得了22种鱼的5S rDNA,运用DNAman软件构建了23种鱼的系统发育树,对白缘(鱼央)的进化地位进行了初步研究。本研究为阐明白缘(鱼央)在鱼类系统进化中的位置、重复序列在脊椎动物性染色体上的分布状况以及与性别决定与分化的关系,提供了资料积累和分析依据。     

基于18S和ITS-5.8S rDNA基因序列的白色霞水母(Cyanea nozakii)的分子鉴定与检测

采用通用引物PCR扩增法,测定了辽东湾海域的白色霞水母(Cyanea nozakii)螅状体、碟状体及水母体的18S以及ITS-5.8S r DNA序列,同时利用Gene Bank数据库中已有同源序列对其进行序列分析及系统分析。结果显示,白色霞水母3个个体的18S和ITS-5.8S r DNA序列完全一致。白色霞水母样品的ITS-5.8S r DNA序列与Gen Bank中未知真核生物的序列高度相似(≥99%),推测该物种可能是早期发育阶段(卵、浮浪幼虫或碟状体)的白色霞水母样品。霞水母属不同种间18S r DNA序列经比对后同源序列长度为1709bp,多态位点数33个;比对后ITS1同源序列长度为368bp,其中变异位点203个,简约信息位点数178个,单变异位点21个。基于18S r DNA基因序列的霞水母属种内和种间平均遗传距离分别为0、0.008,而基于ITS1序列的霞水母属种内和种间平均遗传距离分别为0.019、0.284。基于ITS1的种间遗传距离是种内遗传距离的15倍,适合于进行物种鉴定。NJ系统树的结果也表明同种的不同个体各自聚枝,其聚类结果大致与形态分类吻合。研究表明,ITS基因片段在霞水母不同种间变异较大,更适于大型水母种类鉴定、检测及属内种间水平的系统进化研究。     

Numerical modeling study of mineralization induced by methane cold seep at the sea bottom

In order to investigate the response of authigenic minerals to gas hydrate geo-systems, the biogeochemical processes and its induced mineralization were predicted by employing the comprehensive reactive transport modeling approach. Based on the available data extracted from the northern continental slope area of the South China Sea, a 1-D vertical column model was developed. Three cases with different upward methane flux rates and three cases with different mineral compositions, i.e., a total of six cases were designed to investigate the effects of variations in the depth of sulfate methane transition zone (SMTZ) and in the mineral composition on the formation of authigenic minerals. The simulation results indicate that the SMTZ depth influenced by both the upward methane flux rate and the initial composition played an important role in the formation of authigenic minerals. The AOM reaction is intensive at the interface, and the precipitation amount of calcite is large, which is mainly controlled by AOM. When the methane leakage rate is 20 times higher than the base case, aragonite starts to precipitate. During the simulation, oligoclase, k-feldspar, smectite-Na, smectite-Ca, chlorite dissolved. Our study specific to this area as a starting point may provide a quantitative approach for investigating carbonate and pyrite formation in hydrate-bearing sediments accounting for methane oxidation and sulfate reduction. The method presented here and the model built in this study can be used for other sites with similar conditions. In addition, this study may serve as an indication for the potential natural gas hydrate reservoir in depth, and is also significant for marine carbon and sulfur cycle.     

The Legacy of Jedidiah Morse in Early American Geography Education: Forgotten and/or Forgettable Geographer?

Despite numerous and significant writings by historians of geography and biographers from other disciplines, and his authorship of the first geography textbooks written in and for the new American republic, most geographers are largely unaware of the contributions of Jedidiah Morse in academic geography. Writings about Morse suggest that he had alienated himself from many of his contemporaries early in his career through his authoritarian brand of Calvinistic republicanism, a perceived contradiction of that style with his entrepreneurial ambitions, his role in the controversial Bavarian Illuminati, and a dispute with a noted New England historian. But subsequent, broader intellectual movements sealed Morse's fate as a forgotten geographer (to most), including the end of the Second Great Awakening, Transcendentalism, Darwinism, and the “new,” process‐based geographical thinking inspired by Carl Ritter, Alexander von Humboldt, and Arnold Guyot. Regardless of the reasons for Morse's lost legacy, his contributions to geographical education are important and should be remembered.     

Using climate to relate water discharge and area in modern and ancient catchments

Models relating sediment supply to catchment properties are important in order to use the geological record to deduce landscape evolution and interplay between tectonics and climate. Water discharge (Q _w) is an important factor in the widely used ‘BQART ’ model, which relates sediment load to a set of measurable catchment parameters. Although many of the factors in this equation may be independently estimated with some degree of certainty in ancient systems, water discharge (Q _w) certainly cannot. An analysis of a world database of modern catchments with 1255 entries shows that the commonly applied equation relating catchment area (A ) to water discharge (Q _w = 0·075A^0·8) does not predict water discharge from catchment area well in many cases (R ² = 0·5 and an error spanning about three orders of magnitude). This is because the method does not incorporate the effect of arid and wet climate on river water discharge. The inclusion of climate data into such estimations is an opportunity to refine these estimates, because generalized estimates of palaeoclimate can often be deduced on the basis of sedimentological data such as palaeosol types, mineralogy and palaeohydraulics. This paper investigates how the relationship between catchment area and river discharge varies with four runoff categories (arid, semi‐arid, humid and wet), which are recognizable in the geological record, and modifies the coefficient and exponent of the above‐mentioned equation according to these classes. This modified model yields improved results in relating discharge to catchment area (R ² = 0·95 and error spanning one order of magnitude) when core, outcrop or regional palaeoclimate reconstruction data are available in non‐arid systems. Arid systems have an inherently variable water discharge, and catchment area is less important as a control due to downstream losses. The model here is sufficient for many geological applications and makes it possible to include variations in catchment humidity in mass‐flux estimates in ancient settings.     

三峡库区四方碑滑坡稳定性与变形趋势预测

三峡水库建成后,库水位周期性涨落和暴雨产生的渗流作用导致大量古滑坡的复活或新滑坡的发生。以库区近水平层状结构的四方碑滑坡为例,依据库水位实际调动,将水位从175 m至145 m不同降速与50年一遇暴雨进行工况组合,计算4种工况下滑坡的稳定性及破坏概率。然后采用Geo-studio软件的Sigma模块对滑坡进行变形模拟,运用R/S分析方法判断滑坡的变形持续性,并结合野外调查情况,综合评价分析四方碑滑坡的稳定性。结果表明:滑坡在各工况下整体均处于基本稳定状态,具有低危险性;变形模拟结果显示滑坡前缘位移最大,与野外调查情况一致;各监测点Hurst指数均介于0.5~1,表明时间序列具有正持续性,在研究的时间限度内滑坡的局部破坏增强,应在汛期加强对滑坡前缘的巡查和预警。     

CINRAD-SA双偏振雷达资料在降水估测中的应用初探

对基于水平反射率ZH和差分传播相移率K_(DP)的降水估测综合法R(C)进行了改进,并对广州S波段双偏振雷达2016年2次飑线和2次台风降水过程的Φ_(DP)使用小波分析进行滤波处理,在此基础上使用变距最小二乘法拟合得到K_(DP)的值。分别使用R(C)和R(Z_H)法对2次飑线和2次台风降水过程进行降水估算,将估算结果和雨量计小时雨量进行了对比,并将两种方法的评估结果进行了对比。结果表明:(1)对于飑线类型降水,R(C)法对5 mm·h~(-1)以上的降水估测精度要好于R(Z_H)法,且降水率越大,R(C)法优势越明显,当降水率≥20 mm·h~(-1)时,两次过程R(C)法比R(Z_H)法的平均相对误差(RE)降低了17. 2%,平均绝对误差(AE)减少了1.89 mm,平均均方根误差(RMSE)减少了1.66 mm;(2)对于台风类型降水,R(C)法对5 mm·h~(-1)以上的降水估测精度也好于R(Z_H)法,当降水率≥20 mm·h~(-1)时,两次过程R(C)法比R(Z_H)法的平均RE降低了33. 19%,平均AE减少了3. 95 mm,平均RMSE减少了4.05 mm;(3)对于飑线和台风两种类型降水R(C)法都明显改善了降水率较大时的R(Z_H)法低估问题,但R(C)法在降水率10 mm·h~(-1)时也存在低估,可能是由雨滴谱资料观测误差导致拟合的系数偏小或雷达硬件造成的观测偏差等造成的。     

"3S"技术在城市规划专业的应用与教学改革

信息技术的发展,城市规划专业教育逐步将定量和数字技术方法引入教育体系,文章对“3S”技术特别是“GIS”、“RS”和数字城市技术在城市规划领域的广泛应用和课程体系的教学改革进行探讨和建议。