大口黑鲈(Micropterus salmoides)致病性维氏气单胞菌的分离鉴定及其特性分析

大口黑鲈(Micropterus salmoides)是我国重要的淡水养殖经济鱼类品种,目前包括细菌性疾病在内的诸多因素影响了其养殖业的健康发展。维氏气单胞菌是引起淡水鱼败血症和溃疡综合征的重要病原菌。从患病大口黑鲈肝脏组织中分离到1株病原菌,通过形态学观察、生理生化特性分析、16S rRNA序列和特定毒力基因分析对其进行鉴定,并通过人工回归感染试验和药敏试验分析其致病性和耐药性。结果表明该菌为致病性维氏气单胞菌(Aeromonas veronii),含有aer、act、lip、fla、gcaT和OMPA I等毒力基因;药敏试验显示其对头孢曲松、氯霉素、氟苯尼考、强力霉素、四环素、多粘菌素、复方新诺明、左氟沙星等8种抗生素高度敏感,对新霉素、恩诺沙星、庆大霉素、链霉素、阿齐霉素、环丙沙星等6种抗生素中度敏感,对其他8种抗生素具有一定耐药性;人工回归感染实验表明该菌具有较高的致病性,对大口黑鲈的半致死浓度为1.4×10⁶ CFU/mL。维氏气单胞菌是水产养殖中的条件性致病菌,研究结果揭示了该菌株的部分生物学特性,有利于丰富维氏气单胞菌属的基础数据,同时也为鲈鱼养殖过程中的病害防控提供了一定的参考依据。     

夜间气象能见度的测量研究

运用能见度理论和双止偏光能见度测定仪原理，讨论了夜间气象能见度的测量原理和一种新的测量方法，并完成了初步的野外对比试验，得出了比较结论。     

基于CRU资料的山西百年气温时空演变特征

全球变暖的背景下,百年尺度上的区域气温变化规律,近年来已成为气候变化研究的热点。本文基于1901-2016年英国东英吉利(East Anglia)大学气候研究中心(Climatic Research Unit,CRU)提供的高分辨率、逐月气温数据集,采用一元线性回归法、滑动平均法和Mann-Kendall突变检验法分析了116年来山西气温的时空分布特征。结果表明:百年来山西年平均气温和各季节气温均呈波动上升趋势,年均和各季节气温倾向率分别为0.13℃·(10a)^-1(年平均)、0.16℃·(10a)^-1(春季)、0.05℃·(10a)^-1(夏季)、0.09℃·(10a)^-1(秋季)、0.22℃·(10a)^-1(冬季),其中冬季气温增幅最大,对年均温增长的贡献最大,贡献率为42.31%,夏季气温增幅最小,贡献率也最小(9.62%)。研究时段内,山西各季节气温均发生突变,春季季均温突变开始于1993年,夏季在1917年和1996年都发生了气温突变,秋季、冬季出现突变的年份分别为20...     

黄骅电厂取水河段取水特性的数模与物模研究

本文采用数值模拟与物模试验相结合的研究方法，详细地研究了黄骅电厂一、二、三期工程的取水特性。结果表明，现有河道只能满足电厂一期工程的取水需求；若将现有河道进行适当开挖，可以满足电厂三期取水需要。     

快速加固吹填土的室内模拟试验

吹填造陆是解决沿海地区建设用地不足的主要方法之一。为了使吹填土在较短的时间内获得较好的加固效果,结合吹填土特殊工程地质性质和加固过程中强度增长具有阶段性的特点,提出了边吹填边加固的加固思路。在该思路的指导下进行室内模拟试验。试验发现不同加固阶段吹填土强度增长表现出明显的分带性;与竖向排水体距离小于25 cm的土体强度增长较快;随着加固时间的延长,待加固土体强度整体增长;当持续真空加荷一个月时,部分待加固土体承载力已达到80 kPa。可以看出,强度增长与外部荷载的大小、渗透路径的长短、附加应力的大小密切相关。分阶段加固的方法在一定程度上抑制了泥皮的形成,缩短了真空加荷时间,节约工期,有利于吹填土的加固。     

HUMBOLDT AND THE HABITABILITY OF CANADA'S GREAT NORTHWEST*

ABSTRACT. Alexander von Humboldt's influence in British North America during the nineteenth century was filtered mainly through British imperial applications of “Humboldtian” sciences, including geomagnetism and biogeography. The best‐known examples include Edward Sabine and John Henry Lefroy, Royal Artillery officers who, during the 1830s and 1840s, transformed British North American outposts and territories, including Rupert's Land, into Humboldtian sites and regions in Great Britain's imperial “magnetic crusade.” Important groundwork had already been laid by John Richardson, who applied data accrued during John Franklin's overland Arctic expeditions during the 1820s to systematize Humboldtian inquiries into the habitability of Canada's Great Northwest. Despite both the relative decline of Humboldtian sciences by midcentury and Humboldt's own reservations about the political ramifications of his science, his “cosmic” outlook circulated in Canada to refine territorial expansionists' scientistic arguments justifying annexation of Rupert's Land after the monopoly of the Hudson's Bay Company expired in 1869.     

Decumbent development: Urban sprawl in the Guwahati Metropolitan Area,India

Urban sprawl has become a global phenomenon as an outcome of growing population and rapid urbanization. Previous studies have addressed the rising incidence of uncontrollable urban development, particularly in peri-urban areas of cities, leading to chronic urban sprawl. The city of Guwahati, a million city in north east India, has expanded significantly in recent years. In this article, the links between population and growth of built-up areas were examined using geo-spatial techniques and remotely sensed datasets. The results indicate that the sprawl has accentuated in recent years. The intensity of land use remained uneven due to marked variations in the distribution of built-up areas, plausibly an outcome of unplanned urban growth. If current trends are anything to go by, future urban sprawl could pose serious threats to the vulnerable eco-sensitive and peri-urban areas of Guwahati. Secondary cities have unfortunately received scant attention in urban policy research, and Guwahati, epitomizes urban woes in a developing country.     

Molecular identification of ascaridoid nematodes from the deep-sea onion-eye grenadier (Macrourus berglax) from the East Greenland Sea

Macrourus berglax from the East Greenland Sea was studied for the presence of ascaridoid nematodes in 2001, 2002 and 2003. The fishes were collected between 278 and 413 m water depth using a benthopelagic net. Based on the amplification of the internal transcribed spacer ITS-1, 5.8S, ITS-2 and flanking sequences (=ITS+), three ascaridoid nematode species were identified. The prevalence of infestation during the 3 years ranged from 42.9% to 62.9% and 22.9% to 40.0% for the anisakids Anisakis simplex (s.s.) and Pseudoterranova decipiens (s.s.), respectively, and from 28.6% to 60.0% for the raphidascarid Hysterothylacium aduncum. A total of 18 specimens, two of each species and examination year, revealed no sibling species, suggesting a limited distribution of other ascaridoid siblings into the deep sea. The ITS-1, 5.8S and ITS-2 sequences of A. simplex (s.s.) from the East Greenland Sea did not differ from previously published sequence data (GenBank) from other regions in the Atlantic and Pacific oceans. The sequences of P. decipiens (s.s.) corresponded most closely to those of specimens from Richardson Bay, western Pacific, and differed in four positions (0.5%). They corresponded least to those of specimens from Japan (1.5%). The sequence data for H. aduncum differed in two positions in the ITS-1 (0.2%) and three positions in the ITS-2 (0.3%) from sequences from Japan. A high genetic similarity between the regions can be explained by (a) extensive final host migration in the case of A. simplex (s.s.), (b) an overlapping distribution of final host populations along the continental shelves for P. decipiens (s.s.) and (c) a low host specificity and large population size in the intermediate and final hosts for H. aduncum. The occurrence of the identified species in the macrourid fish underlines the potential of cosmopolitan ascaridoid nematodes to distribute not only horizontally but also vertically in the deep sea.     

Analysis of formation pressure test results in the Mount Elbert methane hydrate reservoir through numerical simulation

Targeting the methane hydrate (MH) bearing units C and D at the Mount Elbert prospect on the Alaska North Slope, four MDT (Modular Dynamic Formation Tester) tests were conducted in February 2007. The C2 MDT test was selected for history matching simulation in the MH Simulator Code Comparison Study. Through history matching simulation, the physical and chemical properties of the unit C were adjusted, which suggested the most likely reservoir properties of this unit. Based on these properties thus tuned, the numerical models replicating “Mount Elbert C2 zone like reservoir”, “PBU L-Pad like reservoir” and “PBU L-Pad down dip like reservoir” were constructed. The long term production performances of wells in these reservoirs were then forecasted assuming the MH dissociation and production by the methods of depressurization, combination of depressurization and wellbore heating, and hot water huff and puff. The predicted cumulative gas production ranges from 2.16 × 10⁶ m³/well to 8.22 × 10⁸ m³/well depending mainly on the initial temperature of the reservoir and on the production method.This paper describes the details of modeling and history matching simulation. This paper also presents the results of the examinations on the effects of reservoir properties on MH dissociation and production performances under the application of the depressurization and thermal methods.     

A review of the equations used to predict the velocity distribution within a ship’s propeller jet

Predicting the velocity within the ship’s propeller jet is the initial step to investigate the scouring made by the propeller jet. Albertson et al. (1950) suggested the investigation of a submerged jet can be undertaken through observation of the plain water jet from an orifice. The plain water jet investigation of Albertson et al. (1950) was based on the axial momentum theory. This has been the basis of all subsequent work with propeller jets. In reality, the velocity characteristic of a ship’s propeller jet is more complicated than a plain water jet. Fuehrer and Römisch (1977), Blaauw and van de Kaa (1978), Berger et al. (1981), Verhey (1983) and Hamill (1987) have carried out investigations using physical model. Current paper reviews the state-of-art of the equations used to predict the time-averaged axial, tangential and radial components of velocity within the zone of flow establishment and the zone of established flow of a ship’s propeller jet.