黑鲷DMRT1基因cDNA片段的克隆和序列分析

根据其他鱼类DMRT1基因中的保守序列设计了一对简并引物 ,利用反转录 多聚酶链式反应 (RT PCR)的方法克隆了黑鲷 (Acanthopagrusschlegeli)DMRT1基因cDNA的一个片段 .该片段长 13 8bp ,推导的氨基酸序列由 45个氨基酸残基组成 .同源性分析表明 ,该cDNA片段与其他鱼类DMRT1基因序列具有较高的同源性     

Application of Subbottom Profiler for Coastal Engineering

Experimental studies have been conducted on the acoustic effects of KLEIN-510 subbottom profiler for different sedimentary layers in estuary and bay areas. Sedimentary layer boundaries can be recorded clearly and continuously by this instrument and then compared with the results from drilling cores to reveal some variations of sedimentary environments. With this instrument, the average deposit rate can be determined and the bed-rock beneath the sea bottom can be found out.     

Attenuated basin‐margin sequence stratigraphy of the Palaeoproterozoic Calvert and Isa Superbasins: The Fickling Group,southern Murphy Inlier,Queensland

Sedimentary rocks of the Palaeoproterozoic Calvert and Isa Superbasins are exposed across a large area of northern Australia. Despite the extent of the exposures there is little to indicate the nature of the basin margins as most outcrop boundaries are structurally or erosionally defined, or the margins, where preserved, are concealed beneath younger basins. The Murphy Inlier, which forms the boundary between the Mt Isa and McArthur Basins, is unique in that on its southern flanks a basin‐margin succession is well‐preserved as the Fickling Group. A detailed sequence‐stratigraphic analysis of outcrop sections and well logs, supplemented by seismic reflection profiles and SHRIMP U–Pb zircon ages, shows that all seven supersequences of the Isa Superbasin and one supersequence from the older Calvert Superbasin are represented in the Fickling Group. Through this high‐resolution sequence‐stratigraphic framework, it is possible to accurately correlate chronostratigraphically equivalent strata from the McNamara Group on the central Lawn Hill Platform to the Fickling Group on the southern Murphy Inlier. Each supersequence thins substantially from the McNamara Group (～11 km thick) to the Fickling Group (<1 km thick). The combined effects of truncation and onlap of sequences over the Murphy Inlier basement high are responsible for the thinning. Major time breaks of up to 25 million years occur between supersequences in the Fickling Group. Erosional hiatuses are often manifested at the base of supersequences as conglomerate beds composed of silicified detritus from older strata. Sequences in the Fickling Group were generally deposited in a proximal basin‐margin setting, while sequences in the McNamara Group were deposited in distal basin depocentres. The proximal depositional setting of Fickling Group sequences reduces the number of thick carbonaceous shale and siltstone intervals, which often host Zn–Pb–Ag and Cu deposits in the McNamara Group. Many host sequences from the McNamara Group are also absent in the Fickling Group due to truncation and onlap pinchout. Consequently, the economic potential of Palaeoproterozoic strata on the southern Murphy Inlier is less than equivalent strata from the central Lawn Hill Platform. Despite this, potential does exist for future discoveries of economic mineral deposits in the Mt Les Siltstone and Walford Dolomite units of the Fickling Group.     

Assessment of hydrodynamic simulation results for eco‐hydraulic and eco‐hydrological applications: a spatial semivariance approach

Output from a three‐dimensional numerical flow model (SSIIM) is used in conjunction with high‐resolution topographic and velocity data to assess such models for eco‐hydraulic applications in river channel design and habitat appraisal. A new methodology for the comparison between field measurement and model output is detailed. This involves a comparison between conventional goodness‐of‐fit approaches applied to a spatially structured (riffle and pool) sample of model and field data, and a ‘relaxation’ method based upon the spatial semivariance of model/field departures. Conventional assessment indicates that the model predicts point‐by‐point velocity characteristics on a 0·45 m mesh to within ±0·1 m s⁻¹ over 80% of the channel area at low flow, and 50% of the area at high in‐bank flow. When a relative criterion of model fit is used, however, the model appears to perform less well: 60–70% of channel area has predicted velocities that depart from observed velocities by more than 10%. Regression analysis of observed and predicted velocities gives more cause for optimism, but all of these conventional indicators of goodness of fit neglect important spatial characteristics of model performance. Spatial semivariance is a means of supplementing model appraisal in this respect. In particular, using the relaxation approach, results are greatly improved: at a high in‐bank flow, the model results match field measurements to within 0·1 m s⁻¹ for more than 95% of the total channel area, provided that model and field comparisons are allowed within a radius of approximately 1 m from the original point of measurement. It is suggested that this revised form of model assessment is of particular relevance to eco‐hydraulic applications, where some degree of spatial and temporal dynamism (or uncertainty) is a characteristic. The approach may also be generalized to other environmental science modelling applications where the spatial attributes of model fits are of interest. Copyright © 2005 John Wiley & Sons, Ltd.     

Sequences Characterization of Microsatellite DNA Sequences in Pacific Abalone(Haliotis discus hannai)

1 Introduction Microsatellites or simple sequence repeats (SSRs) are tandemly repeated motifs of one to six bases found in all prokaryotic and eukaryotic genomes analysed to date (Zane et al., 2002). Due to their hyper-variable and co-dominant nature, relatively high abundance and random distribution in the genome, microsatellites are among the most efficient class of molecular markers. Such repeats display high polymorphism because of variation in repeat length and can be rapidly analysed t…     

����ˮ��ˮ���շ������䷢չ����̽��

?????????????????????????????????з???????????????????????????????????????????????????? ???????о???????? 1996??4??21???????ML4.1????????????????????????????????????3????????????????????С??     

Rotation Periods of Nine ROSAT Selected Solar-Type Stars

We monitored 16 X-ray selected young solar-type stars for light variation and found appreciable periodic light variability with amplitudes of a few hundredths of a magni-tude in nine of the objects. Using the method of Phase Dispersion Minimization (PDM) and Fourier analysis (software PERIOD04), the rotation periods of these stars were determined from the photometric data. The rotation periods of all nine stars are shorter than about 3days. It is suggested that, as with the Pleiades cluster, small amplitude light variations are quite common among young solar-type stars with rotation periods around 3 days or less. This gives further evidence for the spin up of solar-type stars predicted by models of angular momentum evolution of pre-main sequence stars.     

Limitations of sequence stratigraphic correlation between marine and continental deposits: a 3D experimental study of unconformity-bounded units

Experiments have been carried out in a model basin 16 × 1·2 × 0·9 m to address the effect of base‐level and discharge changes on actively growing alluvial‐shoreline wedges. Two distinct types of erosive surfaces were investigated: one produced by base‐level fluctuations in the coastal zone and the other by discharge and supply fluctuations in the upstream alluvial basin. In the first experiment, three similar base‐level cycles were simulated keeping sediment supply and basin tilting constant within each cycle, and changing discharge from one cycle to the next. In the second experiment, rises and falls of base level were instantaneous and discharge changes were in phase (high discharge linked to high water levels in the basin). In the third experiment, base level and discharge changed gradually, at different rates and they were out of phase, resembling a typical glacio‐eustatic cycle in which sea‐level rises and falls are linked to increased and decreased discharge, respectively. The resulting stratigraphy of the alluvial to deltaic sedimentary wedge was analysed in terms of the development of unconformities and the evolving depositional geometry. An intervening decoupled zone between parts of the model basin dominated by alluvial processes and that at the coastal zone is identified. Within this decoupled zone, unconformities in the alluvial succession tend to vanish basinward, and base‐level generated coastal unconformities disappear landward. The two types of unconformity can be generated at different times during a glacio‐eustatic cycle, and it is thus erroneous to correlate them, even though they may appear to form a continuous surface. Unconformities within the modelled stratigraphy do not constitute time lines nor do they consistently separate younger from older beds, as they require a significant time to form and they have thick sedimentary packages as depositional correlatives. The experiments also support a fourfold division of sequences, showing the development of a significant sedimentary package during base‐level falls.     

Simultaneous operation of opposing reaction mechanisms: The influence of matrix heterogeneity on post-kinematic garnet crystallization in an inverted metamorphic sequence

Metapelites from the inverted Barrovian sequence in the Sikkim Himalaya (northeast India) are shown to be largely continuous with respect to their bulk rock compositions, microstructures and pressure–temperature–time–deformation (P–T–t–D) histories. However, the upper garnet–lower staurolite zone demarcates a region of microstructurally anomalous post-kinematic garnet populations contained within strongly segregated matrices. The different microstructures within samples from this region cannot be attributed to differences in their thermobarometric histories or bulk compositions, but are instead interpreted to represent an otherwise unexposed level of the Daling Group that is now exposed along a post-metamorphic thrust splay. These heterogeneous samples contain several discrete garnet populations that progressively crystallized with increasing P–T. Garnet populations that experienced the most protracted growth now form complex polycrystals that exhibit crystallographically controlled and morphologically irregular interfaces adjacent to micaceous and quartzofeldspathic domains respectively. Electron backscatter diffraction indicates that these polycrystalline garnet structures contain numerous coalesced porphyroblasts that are structurally uncorrelated across their grain boundaries. However, a crystallographically preferred orientation at the polycrystal scale is interpreted to derive from epitaxial crystallization of early-formed garnet porphyroblasts on precursor mica. Later-nucleated porphyroblasts within polycrystals preferentially concentrated towards quartzofeldspathic domains, with the overall nucleation distribution likely controlled by a complex interplay between chemical heterogeneities, strain partitioning and epitaxial crystallization. The subsequent growth of these polycrystals was equally spatially heterogeneous; it was moderated by differences in the efficiency of grain boundary transfer between quartzofeldspathic and micaceous domains that precluded thin section-scale chemical equilibration. In contrast to samples from Sikkim containing more typical porphyroblastic populations in continuous and disseminated matrices, heterogeneous availability of garnet-forming components within this strongly layered matrix is shown to have resulted in grain-scale variations in growth rates and the spatial juxtapositioning of interface-controlled microstructures and locally equilibrated chemical compositions with those that were transport controlled.     

鄂尔多斯盆地中东部奥陶系马家沟组马五6亚段硬石膏产状类型与成因*

鄂尔多斯盆地中东部奥陶系马家沟组马五₆亚段硬石膏产状类型多样,基于系统的宏观与微观岩石学分析,识别并区分出星散状(A1型)、纹层状(A2型)、致密块状(A3型)、结核状或团块状(A4型)、鸡雏状或角砾状(A5型)等硬石膏产状类型。单井高频旋回和微相分析表明,马五₆亚段发育与潮坪、潟湖、鲕粒滩、砂屑滩、微生物丘等有关的5种高频向上变浅序列,硬石膏的产状类型、产出位置和分布形式等与向上变浅序列有很好的耦合关系。A1型和A2型硬石膏发育于高频向上变浅序列的下部和中部,主要与膏云质或云膏质潟湖相关,属于潟湖相原生化学沉积成因。A3型硬石膏分布于高频向上变浅序列的上部,受膏质潟湖控制,也属于原生化学沉积成因。经典的结核状硬石膏(A4-1)呈随机分布形式产于潮坪沉积序列,为成岩期交代作用或者膏化作用成因,与传统萨布哈交代成因的膏质结核相似。A4-2型硬石膏主要出现在颗粒岩和微生物岩中,主要由富含CaSO₄的下渗卤水在溶蚀孔洞中沉淀形成;A5型硬石膏发育于向上变浅序列的上部和顶部,与高频暴露有关,为准同生期以溶蚀—充填为主的岩溶改造成因。发育完整的单个高频旋回,大致经历了高频快速海侵早期、高频快速海侵晚期至缓慢海退早期、高频缓慢海退晚期和高频海退末期暴露4个演化阶段,形成了与之耦合的海相碳酸盐岩与硬石膏共生序列。这些认识将对马家沟组沉积古环境的再认识具有重要的参考意义。