单井水文地质参数简化计算及意义

单井抽水试验是水文地质勘探中广泛实施的手段,其导水系数 K M 或渗透系数 K 值通常是从抽水结果的涌水量 Q 和井中水位降数据计算求得.但单井中水量的水位降深,多受井结构完善程度的制约,难以反映出井点处含水层真实的水位降.另外,在基岩含水层试验中,常以抽水井揭露所谓含水层的岩性岩层厚度作为含水层厚,这又给 K 值的确定带来一定的偏差.就日常工作中在对抽水成果整理方面所遇的一些问题提出见解,以共讨论     

Dividing the algal soup: is there niche separation between native bivalves (Echyridella menziesii) and non-native Daphnia pulex in New Zealand?

ABSTRACT

New Zealand's native freshwater mussels (kākahi, kāeo) are considered to be in decline, yet the mechanisms driving this are unclear. The widespread establishment of highly efficient filter-feeding, non-native Daphnia in New Zealand lakes may have led to competition for algae with kākahi. We conducted a controlled laboratory experiment to determine whether differences exist in algal resources utilised by kākahi and North American Daphnia pulex. Echyridella menziesii (adults and juveniles) and D. pulex were exposed to mixed algal samples for three hours to determine whether similar algal resources were removed by the grazers. Relative to controls, both adult and juvenile E. menziesii removed a broad range of algal taxa including diatoms, green algae and filamentous species, ranging in size from 33.6 to 348.0?μm. High densities of D. pulex were unable to cause depletion of these same algae species. Our results suggest niche overlap is limited between the two grazers; E. menziesii utilises larger particles than D. pulex.     

Membrane preconcentration of iron in seawater samples and on-site determination in spectrophotometry

A novel method for on-site determination of trace iron was developed using membrane preconcentration and spectrophotometric detection. Fe(Ⅱ)-ferrozine complex was reacted with cetyltrimethylammonium bromide (CTAB) to form a Fe(Ⅱ)-ferrozine-CTAB paired compound, which was collected on a membrane by filtration under vacuum. The membrane was immersed in 2 mL of ethanol-nitric acid and the absorbance of the solution measured for quantitative analysis. Various factors affecting the iron collection and determination were investigated. With different sample preconcentration volumes, the range of determination was broadened to 0.5-120 g/L. The detection limit of this method reached 0.19 g/L and the recoveries were between 97.2 and 109% when the concentration enrichment was about 45. The relative standard deviation (n = 7) was 1.9% for samples containing 10 g/L Fe. Twelve seawater samples were analyzed on-site using the proposed method, and two were also analyzed in inductively coupled plasma mass spectrometry. No significant difference was shown between the two methods by the Student’s t-test. The method has also been used on-site for iron enrichment experiments with phytoplankton and concluded to be simple, accurate and inexpensive.     

大菱鲆(Scophthalmus maximus)低温胁迫耐受性能与体表蛋白组学研究

采用急性低温胁迫实验方法,通过对实验鱼的死亡率、死亡历时、摄食量以及呼吸频率等耐寒指标进行比较分析,并由此分析结果判断幼鱼的低温临界温度为0℃,在此温度基础上,利用双向电泳技术,对大菱鲆(Scophthalmus maximus)幼鱼进行体表蛋白水平上的耐受机理研究。根据低温胁迫组和常温对照组机体表皮蛋白组图谱差异进行比较分析。结果表明,低温胁迫后蛋白发生了显著变化。从中挑选四个差异蛋白点进行肽指纹图谱(MALDI-TOF-MS)分析,采用PMF技术和MASCOT、NCBI网站提供的检索工具进行鉴定,结果显示,低温胁迫组大菱鲆表皮MLC和Mimecan前体蛋白表达显著上调,而typeⅡ角蛋白表达显著下调。大菱鲆对低温胁迫是一个复杂的网络反应,涉及很多蛋白质的参与,这些低温响应蛋白在大菱鲆对低温胁迫的抗性反应中起到重要的作用。     

逐步判别分析法在海洋水质评价中的应用

针对粤西海域海洋水质评价这一问题,依照GB 3097-1997《国家海水水质标准》建立一个由400个站位组成、每个站位包含13个水质指标信息的假设随机样本,利用逐步判别分析法,在随机样本的13个水质指标中按其对水质分类判别能力的大小进行筛选,最终选出化学需氧量、石油类、镉、总磷、总氮这5个具有显著判别能力的指标,并利用这5个指标建立判别函数及水质分类图版,进而克服水质指标数据引入过多对水质评价模型的不利影响。利用上述水质分类图版对粤西海域2010年40个站位的水质状况进行了评价,对比海洋与渔业环境监测站的水质分类结果,其正确率为95%,说明该水质分类图版具有良好的实际应用效果,可作为解决利用多水质指标信息综合评价海洋水质这一问题的有效方法。     

一种用于三维辐照剂量检测的光学CT技术

放射治疗已经发展到3D适形和调强阶段,使得剂量不但具有3D空间分布而且随时间变化,传统的3D水箱的静态测量方式已经不能满足剂量验证的要求。本论文提出了用组织等效性好的辐射敏感材料制成模体,模体辐照后产生辐射效应并存储空间剂量分布信息,然后采用OCT读出剂量分布,从而完成测量任务,发展成3D凝胶剂量学。本课题设计研制了用于聚合物凝胶模体3D剂量检测的光学CT系统,采用锥束投照扫描,帧投影采集方式,对约580 nm波长可见光的衰减系数进行图像重建,并在此基础上描绘等剂量图等放疗临床需要的剂量学资料。OCT的扫描重建时间,分辨能力,剂量响应等都得到满意的结果。采用自制的MAGIC测量模体,用6 MV医用加速器的X射线进行照射,空间剂量分布信息存储稳定,并得到满意的剂量图。     

汾河特大桥大直径超深旋挖钻孔灌注桩施工技术

蒙华铁路汾河特大桥桩基具有桩径大、钻孔深、地层复杂等特点。在施工过程中遇到了钻孔成孔后缩径、沉渣超标、钢筋笼位移等问题。通过认真分析探索,总结出了旋挖钻机在松散砂层施工条件下的泥浆配置方法、膨胀土缩径现象的处理措施、防止钢筋笼位移的有效工具,有效保障了工程质量、降低了施工成本,产生了一定的社会效益和经济效益。     

香港巨牡蛎对3种浮游植物摄食率和滤清率的研究

本文在室内条件下研究了大、中、小3种规格的香港巨牡蛎（Crassostrea hongkongensis）对牟氏角毛藻（Chaetoceros muelleri）、亚心形扁藻（Platymonas subcordiformis）、球等鞭藻（Isochrysis galbana）3种浮游植物在同等密度、同等生物量条件下的摄食率和滤清率,探讨了香港巨牡蛎对3种浮游植物的摄食差异及其影响因素。结果表明,在28℃水温条件下,香港巨牡蛎的单位个体滤清率和单位体质量滤清率分别为1.40~8.94 L/（ind·h）和0.86~3.17 L/（g·h）,等密度和等生物量浮游植物条件下香港巨牡蛎均表现出单位个体滤清率由高到低依次为:大规格、中规格、小规格;不同规格香港巨牡蛎的单位体质量滤清率相近。相同藻类密度条件下,香港巨牡蛎的单位体质量滤清率由高到低依次为:亚心形扁藻、球等鞭金藻、牟氏角毛藻;相同生物量条件下,香港巨牡蛎对3种浮游植物单位体质量滤清率由高到低依次为:球等鞭金藻、亚心形扁藻、牟氏角毛藻。香港巨牡蛎对不同浮游植物种类和密度的滤清率、摄食率差异主要是受饵料的大小、营养质量和密度等因素影响。     

Effect of high salinity on cell growth and protein production of Antarctic ice microalgae Chlamydomonas sp. ICE-L

Antarctic ice microalgae Chlamydomonas sp. ICE-L can survive and thrive in Antarctic sea ice. In this study, Chlamydomonas sp. ICE-L could survive at the salinity of 132‰ NaCl. SDS-PAGE showed that the density of 2 bands (26 and 36 kD) decreased obviously at the salinity of 99‰ NaCl compared to at the salinity of 33‰ NaCl. The soluble proteins in Chlamydomonas sp. ICE-L grown under salinity of 33‰ and 99% NaCl were compared by 2-D gel electrophoresis. After shocking with high salinity, 8 protein spots were found to disappear, and the density of 28 protein spots decreased. In addition, 19 protein spots were enhanced or induced, including one new peptide (51 kD). The changes of proteins might be correlated with the resistance for Chlamydomonas sp. ICE-L to high salinity.     

Denaturing gradient gel electrophoresis shows that bacterial communities change with mid-ocean ballast water exchange

Ships carry ballast water for better stability and to control trim. However, the discharge of ballast water near ports is known to transport invasive species from one coastal area to another. The exchange of ballast water on the high seas is supposed to reduce such invasions of exotic species. In this study, we used denaturing gradient gel electrophoresis (DGGE) to analyze the composition of the bacterial community in ballast water before and after such a mid-ocean exchange, and we also measured total bacterial counts. Our findings confirmed that the ballast water was replaced by the mid-ocean exchange, as indicated by the marked change in the composition of the bacterial community. There was also a significant decrease in bacterial abundance after the mid-ocean exchange. Finally, our findings support the incubation hypothesis, because the composition of the bacterial communities changed over time within the same ballast water.