浅谈信息化测绘在土地利用与管理中的应用

当今社会,土地的利用与管理工作与人们的日常生活密切相关,小到居民住房的购买,大到国家的土地利用总体规划,其工作成效直接影响着人民生活的安居乐业和国家的长治久安。随着测绘相关技术的日臻完善,我国的测绘体系经历了由传统测绘向数字化测绘的转变,目前正在向信息化测绘过渡。信息化测绘是测绘行业发展的必然趋势,其高效的作业模式必然会给土地的利用和管理提供巨大的便利。首先介绍了信息化测绘的内涵及技术体系,然后探讨了信息化测绘技术在土地利用和管理中的应用,最后对信息化测绘技术在土地利用与管理上的发展前景进行了展望。     

四川芦山7.0地震和汶川8.0地震震源区地壳岩石圈变形特征分析

地壳和岩石圈变形特征研究对于深入了解中强地震的深部孕震环境具有重要科学意义.本文联合P和S波远震接收函数偏移成像结果,对发生过芦山7.0地震和汶川8.0地震的龙门山断裂带及附近区域地壳和岩石圈结构进行分析.结果揭示出在青藏高原向四川盆地过渡的龙门山断裂带,Moho面和岩石圈底界面(LAB)呈现出强烈变形,特别是芦山地震和汶川地震震源区下方地壳出现了错断、下凹,岩石圈也呈现下凹变形特征.这种地壳及岩石圈变形所代表的高应力的积累可能是汶川和芦山地震发生的重要深部地球动力学背景.     

土水特征曲线的通用数学模型研究

土水特征曲线对于研究非饱和土的物理力学特性至关重要。根据土水特征曲线可以确定非饱和土的强度、体应变和渗透系数 ,甚至可以确定地下水面以上水份分布。由于土体物理力学特性的差异 ,导致描述其土水特征曲线的数学模型也各不相同。因此 ,建立土水特征曲线的通用数学表达式 ,显得尤为必要。本文对土水特征曲线数学模型进行了研究 ,依据这些模型的数学表达式形式 ,将其划分为 4种类型。分别由这 4种类型的数学模型推导出具有统一表达式形式的土水特征曲线通用数学模型 ,并运用陕北高原黄土土水特征曲线试验数据对通用数学模型进行了研究     

Investigating the molecular systematic relationships amongst selected Plesionika (Decapoda: Pandalidae) from the Northeast Atlantic and Mediterranean Sea

Despite the high number of species and ecological diversity of pandalid shrimps, there has been no previous attempt to resolve evolutionary relationships of several genera using molecular tools. Although mitochondrial DNA cytochrome oxidase I (COI) is widely used in barcoding studies to delimit species boundaries, additional insights into phylogenetic affinities can be obtained, especially when used in combination with data from additional genes. The knowledge of molecular diversity is essential to understand phylogenetic relationships and will help systematic clarifications. Based on partial fragments of the 16S and COI genes, we have focused specifically on addressing the systematic relationships of the economically and ecologically important shrimp genus Plesionika within a framework of five genera from within the Pandalidae. Our results showed that species within Plesionika are substantially divergent when compared with other genera, exhibiting the highest average nucleotide divergence, with 0.1123 and 0.0846 in COI and 16S genes, respectively. In addition, sequence divergence was found to vary greatly within the genus Plesionika (COI/16S): 0.0247/0.0016 between Plesionika antigai and Plesionika heterocarpus and 0.1616/0.098 between Plesionika heterocarpus and Plesionika edwardsii. We did not find amino acid sequence divergence between P. heterocarpus and P. antigai compared with P. heterocarpus and P. edwardsii (8.10%, K2P distance). Three species of Plesionika (P. antigai, P. heterocarpus and Plesionika scopifera) appear well separated from other Plesionika species in both maximum likelihood and Bayesian analyses. The present study confirms the utility of COI over 16S as a genetic marker to resolve relationships between different species of Plesionika from the Northeast Atlantic and Mediterranean Sea, in addition to species delimitation. The findings highlight the need to further review paraphyly within Plesionika in an attempt to recognize a concordance in the evolutionary history of Plesionika with major ecological and geological events.     

中华蛸寄生丛集球虫(顶复亚门:丛集球虫科)新种的形态学与分子生物学研究

丛集球虫(顶复亚门:类锥体纲:丛集球虫科)是头足类消化道中一类常见寄生虫,在福建连江海域以及浙江南麂岛周边海域采捕到的中华蛸(Octopussinensis)盲囊及肠组织内发现一丛集球虫新种,命名为多刺丛集球虫Aggregata octaculeata sp. nov.。光学显微镜和扫描电镜下观察发现,中华蛸体内的多刺丛集球虫存在配子生殖阶段和孢子生殖阶段,其中,孢子生殖阶段成熟卵囊呈近球形或椭球形,长径653.6—806.5μm,短径588.1—787.1μm。孢子囊为卵球形或椭球形,长径22.4—26.1μm,短径20.5—24.6μm,孢子囊壁为双层,外部光滑,内层多刺。孢子囊内子孢子呈卷曲状,数目范围8—13个,子孢子长度为22.2—28.6μm,宽度为3.0—5.0μm。基于18SrRNA基因构建的系统发育树进一步证实该物种隶属于丛集球虫属,并高度支持了属内[(中华丛集球虫A. sinensis+A. octopiana)+A. eberthi]+多刺丛集球虫A. octaculeata的系统发生关系。     

Optimal design of two-dimensional wings in ground effect using multi-objective genetic algorithm

The shape optimization of the 2-dimensional wing in ground effect (WIG) has been performed by the integration of CFD (computational fluid dynamics) and MOGA (multi-objective genetic algorithm). Because of the trade-off between the aerodynamic forces and the height stability, it is difficult to satisfy the design requirements of efficiency and stability at the same time. In this study, the lift coefficient, the lift-drag ratio and the static height stability are chosen as the objective functions to obtain the optimal wing profiles of a WIG craft. An NACA0015 airfoil is used for the baseline model; the aerodynamic characteristics of the base model are compared with that of the optimal solutions. The profile of the airfoil is constructed by four Bezier curves with fourteen control points resulting in the eighteen coordinates, which are adopted as the design variables. The optimal solutions of the multi-objective optimization are not unique but a set of the non-dominated optima: the Pareto frontiers or a Pareto set. As the results of the multi-objective optimization, the forty Pareto optima, which include high-lift, high-efficiency, and more stable airfoils on the edge of the 3-dimensional objective space, are obtained at thirty evolutions of the generation.     

草鱼出血病病毒VP7蛋白基因的人工合成与原核表达

根据GenBank已发表的GCRVVP7蛋白基因序列,设计合成2条特异性引物和19条搭桥引物,通过搭桥PCR人工合成带有3处变异碱基的GCRVVP7蛋白基因整个编码框,经过酶切、连接、PCR鉴定和测序鉴定克隆到原核表达载体pColdTF中,并转化大肠杆菌BL21(DE3),进行IPTG诱导表达和表达产物的SDS-PAGE、Western-blotting分析、纯化以及反应原性的iELISA检测。结果表明,成功人工合成了长831bp的GCRV VP7蛋白基因编码框和构建了VP7蛋白基因的重组质粒pCold TF-VP7,pColdTF-VP7转化菌经IPTG(1mmol/L)诱导3—4h即可获得特异性蛋白VP7重组蛋白的高效表达,VP7重组蛋白相对分子量大小约为73.9ku,与预期大小相符,且与鼠抗GCRV血清有良好反应原性。     

Profiling Planctomycetales diversity with reference to anammox‐related bacteria in a South China Sea,deep‐sea sediment

A systematic assessment of Planctomycetales diversity in a South China Sea, deep‐sea sediment (1657 m) was conducted using the 16S rRNA gene analysis approach. PCR amplification of the samples from seven sediment layers (0.1, 1, 3, 5, 7, 9 and 11 m below the surface sediment) using the primer set Pla‐46‐F/1392‐R showed that the Planctomycetales existed within a limited range of sediment depths (≤ 5 m), and had a decreasing trend in diversity with increasing depth. The majority of the retrieved Pla‐46‐F/1392‐R sequences belonged to Pirellula‐related Planctomycetales, and two sequences retrieved from the 0.1‐m layer (GenBank accession numbers: DQ996944 and DQ996945 ) shared the same anammox‐related signature oligonucleotides and were closely related to commonly recognized anammox organisms. To identify new anammox‐related biomarkers, three primer sets were designed for amplifying the fragments of hydroxylamine oxidoreductase and S‐adenosylmethionine radical enzyme genes, but no related sequences were found. Our multiple 16S rRNA gene primer sets (Journal of Rapid Methods and Automation in Microbiology, 2008, in press) revealed even an higher diversity of Planctomycetales in the 0.1‐m layer of the sediment, especially at genus level. Our data profiled the distribution pattern of Planctomycetales diversity along sediment depths, and provided molecular evidence for the existence of anammox‐related bacteria in a new location, which broadens our understanding of Planctomycetales diversity in deep sea sediments.     

日本鬼鲉不同组织的8 种同工酶谱

采用垂直聚丙烯酰胺平板电泳技术,对象山港野生日本鬼鲉(Inimicus japonicus)的9种组织器官的8种同工酶进行研究,并分析了其酶谱表型。结果表明,琥珀酸脱氢酶(Succinatedehydrogenase,简称SuDH)在所有组织中均存在,组织间差异较小;乳酸脱氢酶(Lactic dehydrogenase,简称LDH)、苹果酸脱氢酶((Malate dehydrogenase,简称MDH)、苹果酸酶(Malic enzyme,简称ME)、酯酶(Esterase,简称EST)、醇脱氢酶(Alcoholdehydrogenase,简称ADH)、过氧化物酶(Peroxidase,简称POD)、超氧化物歧化酶(Superoxide dismutase,简称SOD)则具有明显的组织特异性,且这些酶在表型、分布和活性上均表现出高度的组织特异性。