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31.
采用Matchmaker酵母双杂交系统,将对虾传染性皮下及造血组织坏死病毒(Infection hypodermal and hematopoietic necrosis virus,IHHNV)编码蛋白NS1、NS2和CP序列分别构建到酵母猎物载体p GADT7和诱饵载体p GBKT7上,分别转化至酵母AH109以检测重组猎物载体和诱饵载体的自激活作用及对宿主的毒性作用,发现无自激活作用和毒性作用,随后将重组猎物载体和诱饵载体两两共转至酵母AH109中,涂布于SD/-Leu/-Trp固体培养基上,再点种至SD/-Ade/-His/-Leu/-Trp/X-α-gal固体培养基以鉴定编码蛋白间的相互作用。表型鉴定结果显示,只有共转化有p GADT7-CP/p GBKT7-CP的酵母重组子能够在SD/-Ade/-His/-Leu/-Trp/X-α-gal上生长并显蓝,而其它重组子均不能在其上生长,表明病毒的CP能够自身互作,而其他编码蛋白间无相互作用。为了进一步研究病毒CP自身互作的作用位点,分别从CP的N端和C端截短若干个氨基酸序列,结果发现CP的自身互作是高度敏感的,任何较少氨基酸序列的缺失都将导致其自身互作的丧失。本研究为深入探讨病毒的组装机制和致病机理奠定了理论基础。 相似文献
32.
Police databases hold a large amount of crime data that could be used to inform us about current and future crime trends and patterns. Predictive analysis aims to optimize the use of these data to anticipate criminal events. It utilizes specific statistical methods to predict the likelihood of new crime events at small spatiotemporal units of analysis. The aim of this study is to investigate the potential of applying predictive analysis in an urban context. To this end, the available crime data for three types of crime (home burglary, street robbery, and battery) are spatially aggregated to grids of 200 by 200 m and retrospectively analyzed. An ensemble model is applied, synthesizing the results of a logistic regression and neural network model, resulting in bi-weekly predictions for 2014, based on crime data from the previous three years. Temporally disaggregated (day versus night predictions) monthly predictions are also made. The quality of the predictions is evaluated based on the following criteria: direct hit rate (proportion of incidents correctly predicted), precision (proportion of correct predictions versus the total number of predictions), and prediction index (ratio of direct hit rate versus proportion of total area predicted as high risk). Results indicate that it is possible to attain functional predictions by applying predictive analysis to grid-level crime data. The monthly predictions with a distinction between day and night produce better results overall than the bi-weekly predictions, indicating that the temporal resolution can have an important impact on the prediction performance. 相似文献
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34.
水库浮游植物群落动态的人工神经网络方法 总被引:5,自引:0,他引:5
根据辽宁大伙房水库1980-1997年的水文和湖沼学观测资料,分别建立浮游植物丰度和蓝藻优势度人工视经网络模型,将年降雨量,7-9月平均水温,7-8月入库水量与7-8月库容之比和磷酸盐作为输入,浮游植物量和丰度作为输出,建立浮游植物落消长的人工神经网络模型,将7-9月平均水温,7-8月入出库水量之比,磷酸盐和总氨作为输入,蓝藻优势度作为输出,建立浮游植物演替的人工神经网络预测模型,并进行检验,其模拟值与观测值平均相对误差分别2%和1%,结果表明,人工神经网络方法优于传统的统计学模型,可进行水库浮游植物群落动态的预测预报,并具有较高的精度。 相似文献
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Short-range prediction experiments of the Kuroshio path variabilities south of Japan 总被引:1,自引:0,他引:1
Assimilation and prediction experiments of the Kuroshio path variability south of Japan were conducted to investigate the
predictability of the Kuroshio path. The assimilation and prediction system is composed of an eddy-resolving model and a three-dimensional
variational analysis scheme with vertical coupled temperature–salinity empirical orthogonal function modes. The sea surface
height (SSH) variability and the variations of the Kuroshio path of the assimilation fields are in good agreement with those
observed. The results of the assimilation are then used as the initial conditions for 138 cases of 90-day prediction experiments
conducted from 1993 to 2004. The predictive limit of our system is assessed by the SSH anomaly in the assimilation field and
is found to be around 40–60 days, which is much longer than that of the persistence. The prediction results show good performance
in the transition stage from a straight to a meandering path. For example, a large meandering event that occurred in August
2004 is successfully predicted in a 2-month forecast. Two types of failure cases are investigated. One is a case where the
eastward propagation speed of the meander is faster than a real state. The dynamical response of the model to the assimilation
revealed that an initial shock, caused by the dynamically unbalanced initial condition, induces the fast eastward propagation
of the meander. The other case exhibits an unrealistic meander. In this case, a cold anomaly at an intermediate layer in the
initial condition grows rapidly and results in the unrealistic meander. This implies that the Kuroshio path south of Japan
has a chaotic nature. These facts revealed by the failure cases give us some insight for improving the predictive skill of
the Kuroshio path variability. 相似文献
38.
本文总结了物化探数据彩色成图技术过程,把非线性科学技术引入物化探数据彩色成图编码。其包括三个方面的内容:指出目前物化探彩色成图编码方法的一些缺点;SOFM算法编码技术的实现;指出物化探数据彩色成图编码是一个最优化问题,构造出这个问题的模型后,应用遗传算法求解模型,进行成图编码。 相似文献
39.
利用地震活动因子A值,对青海省东部地区(监测能力强)小震活动进行空间扫描,分析了该地区中强以上地震前,地震活动因子A值的空间分布、演化特征及其与目标地震的对应关系,并对该方法的预报效能作出评价。 相似文献
40.
基于TM1300的G.723.1语音编解码器的实时实现 总被引:1,自引:0,他引:1
简要介绍了G.723.1编解码算法原理和TM1300定点DSP芯片,针对该芯片的硬件结构特点,设计了一套运行于TM1300之上的实时语音信号采集-编解码-播放的可行方案。讨论了G.723.1语音编解码器在定点TM1300上实时实现的关键技术及在具体实现过程中的问题,提出了相应的解决方法。试验结果表明,单片的TriMedia1300能实现至少5路实时G.723.1语音编解码。 相似文献