The scavenging of free radical and oxygen species activities and hydration capacity of collagen hydrolysates from walleye pollock (Theragra chalcogramma) skin

Fish skin collagen hydrolysates (FSCH) were prepared from walleye pollock (Theragra chalcogramma) using a mixture of enzymes, namely trypsin and flavourzyme. The degree of hydrolysis of the skin collagen was 27.3%. FSCH was mainly composed of low-molecular-weight peptides and the relative proportion of <1000Da fraction was 70.6%. Free radical and oxygen species scavenging activities of FSCH were investigated in four model systems, including diphenylpicrylhy-drazyl radical (DPPH), superoxide anion radical, hydroxyl radical and hydrogen peroxide model, and compared with that of a native antioxidant, reduced glutathione (GSH). FSCH was also evaluated by water-absorbing and water-holding capacity. The results showed that FSCH was able to scavenge free radical and oxygen species significantly and to enhance water-absorbing and water-holding capacity remarkably. Therefore, FSCH may have potential applications in the medicine and food industries.     

基于空间数据挖掘技术的农用地产能核算数据库的应用研究

为提高农用地产能核算数据库的利用效率,以空间关联规则为基础,在河北省土地产能核算数据库的应用中得到提高河北省农业用地普查结果的利用效率的几条知识规则。利用现有软件,使用GIS软件进行空间数据预处理及空间关系计算。属性数据使用access管理,而关联规则挖掘算法直接应用Clementine。通过数据选取、数据预处理、数据变换、数据挖掘算法实施以及模式解释与评估,从空间数据库中提取用户感兴趣的空间模式与特征、空间与非空间数据的普遍关系及其他一些隐含在数据中的普遍的数据特征,从而得到提高土地产能核算成果利用效率的规律。     

Spatial partitioning between species of the phytoplankton-feeding guild on an estuarine intertidal sand flat and its implication on habitat carrying capacity

The fishery yield of Manila clams, Ruditapes philippinarum, increased considerably in the 1970s but has decreased rapidly since the middle 1980s on extensive intertidal sand flats in Ariake Sound (Kyushu, Japan). A survey conducted in 2004 on a 3.4-km² sand flat located in the central part of the Sound (Shirakawa sand flat) revealed four dominant species: two thalassinidean shrimps (Upogebia major and Nihonotrypaea japonica), which are deep-reaching burrow dwellers with strong bioturbation activities, and two bivalves (Mactra veneriformis and R. philippinarum). All four species belong to a phytoplankton (diatom)-feeding guild. In the late 1970s, the Manila clam population prevailed in high densities over the entire sand flat, whereas its distribution was restricted to the lowest quarter of the shore in 2004. In contrast, the population sizes and zones of occurrence of the other phytoplankton feeders have expanded in the absence of R. philippinarum, perhaps an indication of competitive release. After establishment, effects of the thalassinidean shrimps on sediment stability appear to have further reduced clam abundances. Across the sand flat in 2004, wet weight population biomass estimates for N. japonica, U. major, M. veneriformis, and R. philippinarum (whole body for shrimps and soft tissue for bivalves) were 304, 111, 378, and 234 tonnes, respectively. Based on Manila clam fishery yield records from Shirakawa, the carrying capacity of the Shirakawa sand flat in the late 1970s was estimated to be two times greater than the sum value for the whole phytoplankton-feeding guild in 2004. It is hypothesized that (1) the amount of phytoplankton determines the carrying capacity for the benthic community on the Shirakawa sand flat, with both phytoplankton and benthic biomass at maxima in the late 1970s, and (2) the subsequent increases in competition for space have caused further declines in the Manila clam population biomass to approximately one-eighth of its past value.     

Coastal environmental assessment and management by ecological simulation in Yeoja Bay, Korea

An eco-hydrodynamic model was used to estimate the carrying capacity of pollutant loads and response of water quality to environmental change in Yeoja Bay, Korea. An energy-system model also was used to simulate the fluctuation in nutrients and organic matter in the bordering wetland. Most water quality factors showed a pulsed pattern, and the concentrations of nutrients and organic matter of seawater increased when input loads of nutrients increased due to freshwater discharge. The well-developed tidal zones and wetlands in the northern area of the bay were highly sensitive to input loads. Residence times of water, chemical oxygen demand (COD), and dissolved inorganic nitrogen (DIN) within the bay were estimated to be about 16 days, 43.2 days, and 50.2 days, respectively. Water quality reacted more sensitively to the effects of nitrogen and phosphorus input than to COD. A plan to reduce the present levels of COD and dissolved inorganic phosphorus (DIP) by 20–30% and DIN by at least 50% in pollutant loads is needed for satisfying the target water quality criteria. The natural removal rate of nutrients in wetlands by reeds was assessed to be approximately 10%.     

Oxidation of copper(I) in seawater at nanomolar levels

The oxidation and reduction of nanomolar levels of copper in air-saturated seawater and NaCl solutions has been measured as a function of pH (7.17–8.49), temperature (5–35 °C) and ionic strength (0.1–0.7 M). The oxidation rates were fitted to an equation valid at different pH and ionic strength conditions in sodium chloride and seawater solutions:

The reduction of Cu(II) was studied in both media for different initial concentrations of copper(II). When the initial Cu(II) concentration was 200 nM, the copper(I) productions were 20% and 9% for NaCl and seawater, respectively. The effect of speciation of copper(I) reduced from Cu(II) on the rates was studied. The Cu(I) speciation is dominated by the CuCl₂⁻ species. On the other hand, the neutral chloride CuCl species dominates the Cu(I) oxidation in the range of 0.1 M to 0.7 M chloride concentrations.     

Kinetic and equilibrium studies of copper-dissolved organic matter complexation in water column of the stratified Krka River estuary (Croatia)

An interaction of dissolved natural organic matter (DNOM) with copper ions in the water column of the stratified Krka River estuary (Croatia) was studied. The experimental methodology was based on the differential pulse anodic stripping voltammetric (DPASV) determination of labile copper species by titrating the sample using increments of copper additions uniformly distributed on the logarithmic scale. A classical at-equilibrium approach (determination of copper complexing capacity, CuCC) and a kinetic approach (tracing of equilibrium reconstitution) of copper complexation were considered and compared. A model of discrete distribution of organic ligands forming inert copper complexes was applied. For both approaches, a home-written fitting program was used for the determination of apparent stability constants (K_i^equ), total ligands concentration (L_iT) and association/dissociation rate constants (k_i¹,k_i^- 1).A non-conservative behaviour of dissolved organic matter (DOC) and total copper concentration in a water column was registered. An enhanced biological activity at the freshwater–seawater interface (FSI) triggered an increase of total copper concentration and total ligand concentration in this water layer. The copper complexation in fresh water of Krka River was characterised by one type of binding ligands, while in most of the estuarine and marine samples two classes of ligands were identified. The distribution of apparent stability constants (log K₁^equ: 11.2–13.0, log K₂^equ:8.8–10.0) showed increasing trend towards higher salinities, indicating stronger copper complexation by autochthonous seawater organic matter.Copper complexation parameters (ligand concentrations and apparent stability constants) obtained by at-equilibrium model are in very good accordance with those of kinetic model. Calculated association rate constants (k₁¹:6.1–20 × 10³ (M s)^− 1, k₂¹: 1.3–6.3 × 10³ (M s)^− 1) indicate that copper complexation by DNOM takes place relatively slowly. The time needed to achieve a new pseudo-equilibrium induced by an increase of copper concentration (which is common for Krka River estuary during summer period due to the nautical traffic), is estimated to be from 2 to 4 h.It is found that in such oligotrophic environment (dissolved organic carbon content under 83 µM_C, i.e. 1 mg_CL^− 1) an increase of the total copper concentration above 12 nM could enhance a free copper concentration exceeding the level considered as potentially toxic for microorganisms (10 pM).     

Stochastic evaluation of fluvial to marginal marine sealing facies

Hydrocarbon top seal analyses are particularly difficult in fluvial and marginal marine settings. Although these settings often contain beds comprised of a low-permeability, clay-rich lithology associated with high quality seals, their presence does not guarantee an effective seal because individual beds within the stratal unit may be laterally discontinuous. Lateral facies changes, truncation of fine-grained beds, or the presence of crevasse splays may provide cross-stratal migration pathways, reducing the effective sealing capacity to a magnitude less than the closure height.     

基于公平性的水环境容量分配研究——以沱江流域为例

公平与效益是容量分配的两个重要原则.在我国目前采用的水环境容量无偿分配方式下,公平原则是需遵循的首要原则,在公平的基础上追求效益.以沱江流域为对象,综合考虑涉及区域的社会、经济、自然等客观因素,筛选指标确定系数对流域容量进行分配.应用经济学中衡量收入公平的基尼系数概念,对分配结果的公平性进行评估,给出综合体现社会性、经济性和历史性的水环境容量分配方案.