高效液相色谱-离子阱质谱法测定海洋微藻藻粉中的8种脂溶性毒素

建立了利用高效液相色谱-电喷雾离子阱质谱(HPLC-ESI-IT-MS)测定海洋微藻藻粉中8种典型脂溶性毒素的分析方法。藻粉样品经超声细胞破碎后,采用超声波辅助提取法对藻毒素进行提取,用HPLC-ESI-IT-MS多反应离子监测(MRM)模式对各种毒素(包括大田软海绵酸(OA)、鳍藻毒素1(DTX-1)、扇贝毒素2(PTX-2)、虾夷扇贝毒素(YTX)、原多甲藻酸1(AZA1)原多甲藻酸2(AZA2)、罗环内酯毒素(SPX),米氏裸甲藻毒素(GYM))进行测定。8种脂溶性藻毒素均在线性范围内线性关系良好(R2均在0.991以上),检出限均介于0.085～1.315 pg之间,加标回收率在88.5%～111.4%之间,方法重复性相对标准偏差(RSD)在4.82%～10.17%范围。应用该方法对利玛原甲藻干藻粉中的毒素进行了测定,分析结果良好,说明本方法是海洋微藻藻粉中脂溶性藻毒素测定的有效方法。     

Interactive discovery of sequential patterns in time series of wind data

Wind speed and direction vary over space and time due to the interactions between different pressures and temperature gradients within the atmospheric layers. Near the earth’s surface, these interactions are modulated by topography and artificial structures. Hence, characterizing wind behaviour over large areas and long periods is a complex but essential task for various energy-related applications. In this study, we present a novel approach to discover wind patterns by integrating sequential pattern mining and interactive visualization techniques. The approach relies on the use of the Linear time Closed pattern Miner sequence algorithm in conjunction with a time sliding window that allows the discovery of all sequential patterns present in the data. These patterns are then visualized using integrated 2D and 3D coordinated multiple views and visually explored to gain insight into the characteristics of the wind from a spatial, temporal and attribute (type of wind pattern) point of view. This proposed approach is used to analyse 10 years of hourly wind speed and direction data for 29 weather stations in the Netherlands. The results show that there are 15 main sequential patterns in the data. The spatial task shows that weather stations located in the same region do not necessarily experience similar wind pattern. For within the selected time interval, similar wind patterns can be observed in different stations and in the same station at different times of occurrence. The attribute task discovered that the repetitive occurrences of chosen pattern indicate as regular wind behaviour at different weather stations that persisted continuously over time. The results of these tasks show that the proposed interactive discovery facilitates the understanding of wind dynamics in space and time.     

深水稀井区天然气藏储盖组合定量预测方法研究:以琼东南盆地陵水凹陷为例

南海北部油气勘探表明天然气是本地区主要的资源类型。与石油相比,天然气藏的形成对盖层要求更为严格,因此开展天然气藏储盖组合研究对油气勘探具有重要意义。通过统计和调研的方法,将砂岩储层孔隙度12%定义为琼东南盆地天然气有效储层评价的下限参数,将泥岩排替压力1 MPa定义为天然气有效泥岩盖层评价的下限参数。利用测井资料开展了单井天然气藏储盖组合窗口定量预测,得出陵水凹陷W22-1井发育单个天然气藏储盖组合窗口。在此基础上,针对稀井或无井区,提出了基于地震反演速度剖面的天然气藏储盖组合定量预测方法,以琼东南盆地陵水凹陷主洼过井测线为例,开展了天然气藏储盖组合窗口地震预测,确定出该凹陷主洼发育单个天然气藏储盖组合窗口,大体上包括陵水组-莺歌海组上部多套地层,拓宽了陵水凹陷天然气勘探层系。这种方法对其它深水区和低勘探新区均有借鉴意义。     

Factors influencing undrained strength of fine-grained soils at high water contents

Understanding the undrained strength of fine-grained soils has been of interest to geotechnical researchers from many practical considerations. In several civil engineering applications, water content of soil is quite high being near or above the liquid limit of soils, and understanding the factors responsible for imparting the strength of soil at high water contents is of great significance. Recently, it has been reported in the literature that the shear strength of soils at these limiting water contents has significant variation. However, the reasons and the factors that probably influence for this variation have not been reported in the literature. This experimental investigation is an attempt in the direction of understanding the reasons for the variation in the undrained strength at higher limiting water content, namely liquid limit considering the various influencing factors like clay mineralogy and fine-sand content present in soil used for determining liquid limit. The results from this study are quite revealing and have been explained based on the mechanisms controlling the undrained strength at liquid limit.     

老盐井组井型互换再利用工程实践

水平钻井连通水溶采卤一直是开采井矿盐资源最常用和最经济有效的手段,但由于地层条件复杂、饱和卤水结晶、套管破损等因素造成盐井组采卤运行过程中通道堵塞,一直是制约盐井组运行年限的主要原因。近些年,对堵塞井组修治常用方法有憋压、通井、开窗侧钻,其中水平井开窗侧钻重新对接现有溶腔在很多盐田区块修井中被广泛应用。在河北宁晋石盐田Y2-Y4井组修井过程中,存在老溶腔无法再利用的情况,此时老溶腔成为井组二次对接的“累赘”。在该区块首次采用井组井型互换（水平井改直井,直井改水平井）的方法,避开了老溶腔,实现了老井组的再利用。     

混合被动控制优化设计及性能研究

本文将调谐液体阻尼器(TLD)和黏弹性阻尼器(VED)同时作用于结构,构成混合被动控制系统。通过对两类阻尼器分别进行优化设计并考虑两者间的相互影响,在充分发挥两者各自优良控制性能的同时,克服了VED大量使用导致控制系统整体造价过高的问题。算例分析表明,混合控制可以得到令人满意的整体减震效果,同时大大节约了VED用量,提高了控制系统的综合经济性能。     

水平地震激励下储罐液体晃动与提离分析

在考虑地基与储罐相互作用的情况下,采用有限元法对储罐在水平地震荷载作用下的液面晃动及储罐提离反应进行了分析。结果表明:罐内液体的晃动是长周期运动,其周期受地震动影响。无论是小体积罐还是大体积罐,在一定的地震烈度下均可以发生提离,而且储罐发生提离的时刻大多数是在地震动的峰值过后的一段时间内。大体积的储罐的提离明显小于小体积的罐。底板提离区域为月牙形。     

6种鱼类肌肉组织肌苷酸的检测分析

采用高效液相色谱技术,在国内首次测定了条纹斑竹鲨(Chilocsyllium plagiosum)、大黄鱼(Pseudosci-aena crocea)、鳜(Siniperca chuatsi)、青石斑鱼(Epinephelus awoara)、牙鲆(Paralichthys olivaceus)和乌塘鳢(Bostrichthys sinensis)等6种鱼类新鲜和冻存肌肉组织中肌苷酸含量。结果表明:(1)不同鱼类肌肉组织中肌苷酸质量比不同,牙鲆新鲜肌肉组织中肌苷酸质量比最高,为4.214 mg/g,其余5种鱼类为2.365~3.634 mg/g。(2)10 d冻存试验中,经-70℃超低温冻存,6种鱼类冻存肌肉组织中肌苷酸质量比均高于新鲜肌肉组织,大黄鱼肌肉组织冻存1 d肌苷酸质量比达最大值,鳜肌肉组织冻存10 d肌苷酸质量比呈上升态势未出现最大值,其余5种鱼类肌肉组织肌苷酸质量比均在冻存第5天达到最大值。长时间的冻存将会降低食用鱼肉中肌苷酸质量比,导致鲜味下降,但经1周左右短时间的冻存后可明显提高鱼肉中肌苷酸质量比,增加鲜味,提高食用价值。     

低渗油藏采收率确定新方法

通过对油藏相渗曲线的归一化处理,利用达西定律理论,建立采油指数与含水率的关系;再通过油藏确定的单井经济极限采油量和油藏极限生产压差求出极限采油指数,从而得到极限含水率;最后以最终水驱体积波及系数与储层退汞效率计算出采收率。并以低渗透油藏为实例验证了该方法的正确与适用性,说明该方法对油田开发中确定低渗油藏采收率具有科学的指导意义。     

基于NS2的TCPW性能改进算法研究

TCP Westwood(TCPW)和Vegas都是基于测量的TCP New-Reno增强算法。TCPW针对ACK流直接估算可用带宽,在慢启动和线性递增部分依然采用传统Reno的盲目递增的机制,导致发送端较为频繁的重传。对这几种算法原理进行分析阐述,并提出1种改进方法,更精确的测量估计带宽,通过网络仿真工具NS2进行验证,明显去除TCP全局同步现象,增强网络性能。