The dose-response relationship between No. 2 fuel oil and the growth of the salt marsh grass,Spartina alterniflora

The effect of No. 2 fuel oil on the biomass production of the salt marsh plant, Spartina alterniflora, was studied in a greenhouse dose-response experiment. S. alterniflora were transplanted into soil with 10 dosage levels of No. 2 fuel oil ranging from 0 to 456 mg g(-1) dry soil. Three months after transplantation, values for plant biomass, stem density, and shoot height decreased significantly with increasing fuel oil level in a dose-response fashion. Evapo-transpiration rates were correlated with the total biomass response. Relative to the control, a significant decrease in total (above- plus below-ground) plant biomass was observed at concentrations above 57 mg g(-1) dry soil. Within the 3-month experimental period, detrimental effects on below-ground biomass accumulation and bioluminescence of the marine bacterium Viberio fisheri in the Microtox Solid Phase Test were observed at oil concentrations >29 mg g(-1) dry soil, suggesting that biological effects of oil within the sediment matrix may be more pronounced than on above-ground biomass, requiring a dosage 228 mg g(-1) dry soil to elicit a significant detrimental effect. Hence, measurements of oil effects with biological end-points based solely on above-ground responses may underestimate the potential impacts of petroleum hydrocarbon spills, especially when the oil has penetrated the soil. While S. alterniflora was proved to be relatively tolerant to the No. 2 fuel oil spills, its effectiveness in phytoremediation operations may be limited at fuel oil levels 228 mg g(-1) dry soil, as both plant growth and microbial activity may be constrained.     

Application of the luciferase cell culture bioassay for the detection of refined petroleum products

A luciferase cell culture-based bioassay, developed to detect 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD)-like activity of halo-genated and polycyclic aromatic hydrocarbons, was optimized to detect refined petroleum products and to determine their relative inducing potency. Quality control standards from 32 refined products (gasolines and diesels, jet fuels, lubricating oils, fuel oils and weathered products) and three commercial products were evaluated. Induction equivalents (I-EQs) were determined by direct comparison of the EC50 and EC20 values (based on the median and 20% TCDD maximal response, respectively) from dose-response curves for each product to those obtained with TCDD. Most petroleum products were active in the luciferase bioassay, with those products composed of fractions produced later in the distillation process (i.e. fuel oils) inducing higher levels. Additionally, weathering of products reduced their induction potency. Based on the high I-EQ estimates of many products, biological effects associated with exposure may have been previously underestimated using other diagnostic methods.     

Tar ball frequency data and analytical results from a long-term beach monitoring program

Following the spill of fuel oils from the New Carissa in February 1999, approximately 300 km of beaches on the Pacific coast of North America were surveyed. A long-term observation program focused on the documentation of stranded tar balls in the vicinity of the spill site. Systematic beach surveys which were conducted over the period March 1999 to April 2001 and semi-logarithmic scale, time-series plots proved the most useful format for identifying trends. Beach monitoring continued through to August 2001. by which time 212 tar balls had been analyzed by GC/MS for their chemical characteristics. The samples of tar balls collected between February 1999 and August 2001 were qualitatively compared with New Carissa source oils (NCSO) and 101 (48%) were not consistent with NSCO. The presence of tar balls that are not related to an incident can confound attempts to define cleanup or endpoint criteria and to assess possible injury to natural resources.     

坪北油田延长组长6油组储层层内非均质性研究

在坪北油田应用岩心观察、岩心分析化验资料、测井解释成果、测试及开发动态等方面的资料分析长6储层的非均质性,重点解剖层内非均质性特点,总结了正韵律,反韵律,复合韵律和交互韵律4种类型的层内非均质性。夹层分布较广泛,加剧了层间非均质性。     

华池油田长33低渗油藏流动单元研究

流动单元的研究,其目的在于对油藏的非均质性有进一步的认识,为提高油藏描述的精度提供依据。本文根据流动单元的理论,选取适合该区实际的参数,应用聚类判别分析的方法,对华池油田陇东地区长33低渗储层进行分类研究,并且分析各类流动单元的特征及其控制因素,得出结论:该区储层可分为三类流动单元,各类流动单元的判别公式,不同的类型在动态、静态上有不同的表现;其分布的差异,宏观上受沉积微相的控制,微观上受成岩作用、岩石自身储集空间特征的限制。     

大庆油田葡萄花油层组储层非均质性主控因素分析

本文对萨尔图油田中部葡萄花油层组的非均质性进行定量半定量研究,认为葡萄花油层组PⅠ2小层层内非均质性最严重,与沉积微相密切相关;单砂层垂向上渗透率的变化以正韵律和复合式韵律为主,局部发育反韵律模式;层内非均质性强弱依次为PⅠ2a、PⅠ3、PⅠ2b、PⅠ1、PⅠ4;层间非均质性在PⅠ3和PⅠ4间表现得最强,其它相对要弱;各小层平面非均质性相差无几,整体表现为较强的非均质性。     

黔中降起的油气勘探壁垒——兼论海相碳酸盐岩烃源岩

黔中隆起在地质构造上属于扬子地块的滇黔隆褶带．南华纪晚期初现雏形，震旦纪—奥陶纪为水下隆起，燕山运动使其整体抬高，喜马拉雅运动时急剧隆升，构造地貌长期相对隆起。地表油苗油样分析结果显示，在红外吸收谱图中缩合芳烃结构及含氧基团吸收明显，有机质热成熟度高，且遭受了氧化蚀变：震旦系以上地层大量暴露，地表水溶蚀下渗深度在黔西南地区可达3000-4000m；作为长期存在的古隆起，经历了多次抬升，直至挽近仍处于严重剥蚀阶段。地腹可能存在酸性岩浆热源使有机质过度热演化、保存条件不佳以及缺少勘探目的层，是黔中隆起油气勘探的三大壁垒。兼谈了碳酸盐岩烃源岩问题．认为形成大油气田的烃源岩主要是泥质岩系，“碳酸盐岩烃源岩”只是从属性的．分布在泥质烃源岩系的边缘或局部范围。     

基于Hyperion影像的涩北气田油气信息提取

 对柴达木地区涩北气田地质地理环境下的蚀变矿物进行分析，结合卫星高光谱遥感数据Hyperion的图谱，对已知气田区与背景区光谱特征进行相关分析，确定了932.64~1 346.25 nm与2 002.06~2 385.5 nm为油气信息识别的有利波长范围； 利用光谱角制图（SAM）技术提取了涩北气田油气的空间分布信息和台吉乃尔含气构造等远景区，为高光谱遥感油气勘探提供了有效技术方法与途径。     

人工合成烃类包裹体的实验研究

运用 Sterner 和 Bodnar(1984)提出的愈合裂隙的实验技术,在石英中成功地合成了烃类包裹体,并对合成的包裹体进行了岩相学观察、显微温度测定及激光拉曼光谱分析等。实验揭示出除合成压力之外,合成温度、反应时间和油水比例是烃类包裹体合成时几个需要重点考虑的因素。此外,合成时加入的初始溶液盐度和酸碱度也值得进一步考虑。人工合成烃类包裹体为研究天然烃类包裹体的形成机制及测定分析奠定了基础。     

油气聚集对石英矿物成岩演化的影响

油气在砂岩储层中的聚集会对石英矿物的胶结作用产生影响。对黄骅坳陷三马探区的石英矿物的包裹体分析以及对开放孔隙中原油抽提物和包裹体中原油的生物标志物进行分析,含油级别较低的油层与水层具有相似的均一化温度分布范围,但富含油层中流体包裹体均一化温度高值分布范围比低级别油层和水层低,包裹体中的烃类组成与开放孔隙中的主要存在成熟度上的不同。分析认为石油的充注不会使石英矿物的胶结作用立刻停止,石英的次生加大作用仍在继续,但胶结作用受到一定的抑制,当石油充注到一定程度后,胶结作用将会停止,后期进入储层的成熟度较高的原油可能未被捕获。