Size-fractionated uranium isotopes in surface waters in the Jiulong Estuary in China

Surface water was collected from the Jiulong Estuary for determination of activity concentrations of uranium isotopes in different size fractions, namely, greater than 53, 10 -53, 2 - 10, 0.4 -2 μm, 10 000 u -0.4 μm and less than 10 000 u fractions by microfihration and cross-flow uhrafiltration technologies. Results indicated that most of the dissolved uranium （ 〈 0.4 μm） exis- ted in the low molecular mass fraction （ 〈 10 000 u）, and the colloidal uranium-238 （10 000 u -0.4 μm） only contributed less than 1% of the dissolved uranium-238. The fractions of colloidal uranium in the dissolved phases decreased with the increasing sa- linity. A positive linear relationship between uranium-238 activities and salinities was observed for the dissolved, colloidal and low molecular mass fractions, indicating a conservative behavior of uranium in the Jiulong Estuary. In the particulate phases （ 〉 0.4 μm）, the partitioning of uranium isotopes among different size fractions was controlled by the partitioning of particle concentrations. In the regions with salinities below 20, the partitioning of uranium-238 among different size fractions was as follows： 10 - 53 μm 〉 2 - 10 μm 〉 0.4 - 2 μm greater than above 53 μm. However, the order at the offshore station with salinities above 30 changed as follows ： 0.4 - 2 μm 〉 10 - 53 μm 〉 2 - 10 μm greater than above 53 μm. The fraction of the 0.4 - 2 μm particles increased at the offshore station, suggesting the increased contribution of the authigenic uranium. The activity ratio of uranium-234 to uranium-238 in the dissolved phases, including the low molecular mass fraction and the colloidal fraction, was larger than unity, showing the occurrence of excess uranium-234. In contrast, the activity ratio of uranium-234 to uranium-238 in all size fractions of the particulate phase was close to the equilibrium value （1.0）. The observed different values of the activity ratio of uranium-234 to uranium-238 in the dissolved phase and the partic     

In vivo neutralization assays by monoclonal antibodies against white spot syndrome virus in crayfish(Cambarus proclarkii)

The neutralizing activities of eight monoclonal antibodies （MAbs） against white spot syndrome virus （WSSV） （2D2, 2B2,1D2, 1D5, 1C2, 4A1, 6A4 and 6B4） were analyzed by in vivo experiments. Gills from WSSV-infected shrimp were homogenized and ten-fold serially diluted by PBS, and then incubated with MAbs （hybridoma culture supernatant）, respectively. The mixture of WSSV and MAbs were injected into crayfish （Procambarus clarkii）. After challenge, the death rates of crayfish were counted to determine the neutralizing activities of MAbs. At the same time, the mixture of myeloma culture supernatant and WSSV or PBS was served as positive or negative control, respectively. The results showed that, at each virus dilution, the mean time to death of the crayfish injected with MAb-treated virus was significantly longer than that in the positive control, though they all showed 100% mortality within 25 d, and meanwhile, few crayfish died in the negative control. Among the eight MAbs, 2D2, 2B2, 1D2 and 1D5, especially the former two, delayed the mortality significantly, and 1 C2, 4A1 and 6A4 delayed the mortality as well but not so efficiently, while MAb 6IM was efficient only when the virus concentration increased. The results indicated that the anti-WSSV MAbs can neutralize WSSV in different virus dilutions.     

利用南极走航观测评估卫星遥感海表面温度

利用1989-2005年间南极走航观测的海表面温度,对目前3个主要的卫星反演的SST产品AVHRR(Advanced Very High Resolution Radiometer),TMI(TRMM Microwave Imager)和AMSR-E(Advanced Microwave Scanning Radiometer for the Earth Observing System)进行了较为系统的评估,并着重检验了它们在南大洋的准确性.结果表明,AVHRR SST比观测数据偏冷,白天的偏差为-0.12℃,夜晚的偏差为-0.04℃,而且南大洋的冷偏差更为显著.TMI SST比观测数据明显偏暖,白天的偏差为0.48℃,夜晚的偏差为0.57℃,其温差ΔT受37GHz风速影响,在强风速(>6m/s)下这种影响仍然存在.AMSR-ESST比观测数据偏暖,白天的偏差为0.34℃,夜晚的偏差为0.27℃,而且南大洋的暖偏差相对较大.AMSR-E SST温差受水汽影响,并在南大洋随着水汽的增加而增加.通过进一步比较微波(AMSR-E和TMI)和红外(AVHRR)遥感的SST在2004年北半球冬季(即南半球夏季)的差别,发现微波遥感在热带(15°S-15°N)和南大洋区域(45°S以南)比红外遥感偏暖,而且在南大洋区域的偏差相对较大,相反在北半球中纬度区域(15°~40°N)偏冷.AMSR-E与AVHRR SST的温差,从白天到夜晚有减小的趋势,而TMI与AVHRR SST的温差无明显的变化.     

原位杂交技术在斜带石斑鱼神经坏死病毒检测中的应用

根据斜带石斑鱼（Epinephelus coioids）神经坏死病毒（orang-spotted nervous necrosis virus,0GNNV）的主衣壳蛋白（major capsid protein,MCP）基因的保守序列,设计一对引物,从感染0GNNV的斜带石斑鱼组织匀浆液提取RNA为模板进行RT-PCR扩增,得到426bp的cDNA片断。用得到的RT-PCR产物加上地高辛（DIG）标记作为核酸探针。通过注射病毒提取液人工感染一组斜带石斑鱼,解剖感染病毒的斜带石斑鱼,从中分离出脑和眼睛,运用原位杂交技术检测组织中的OGNNV。实验表明,原位杂交具有较高的灵敏性和特异性,可以用原位杂交的办法来检测养殖的石斑鱼是否有携带该病毒,达到监控和预防神经坏死病爆发的目的。本实验还采用了H＆E染色方法检测了感染NNV的石斑鱼脑部和眼部组织,观察细胞内的坏死部分。与原位杂交做对比,提高了检测的可靠性和准确性。本实验建立的石斑鱼神经坏死病毒的ISH检测方法有着较高的特异性和敏感性,易于操作,有助于石斑鱼神经坏死病毒的组织定位、发病机理的研究。     

Assessing New England's waterborne energy imports (1995–2004)

This paper tests four hypotheses relating to the waterborne commerce of New England's imported energy, by port of entry, from 1995 through 2004. It concludes that the region's ports engaged in this fossil fuel traffic are part of a hierarchical system of large, medium, and small ports; that such energy flows have increased over the study period; that localized demand for energy is the principal component leading to growth; and that regional inter-port competition was not stable.     

Predicting suitable habitat for the cold-water coral Lophelia pertusa (Scleractinia)

Ecological-niche factor analysis (ENFA) was applied to the reef framework-forming cold-water coral Lophelia pertusa. The environmental tolerances of this species were assessed using readily available oceanographic data, including physical, chemical, and biological variables. L. pertusa was found at mean depths of 468 and 480 m on the regional and global scales and occupied a niche that included higher than average current speed and productivity, supporting the theory that their limited food supply is locally enhanced by currents. Most records occurred in areas with a salinity of 35, mean temperatures of 6.2–6.7  °C and dissolved oxygen levels of 6.0–6.2 ml l⁻¹. The majority of records were found in areas that were saturated with aragonite but had low concentration of nutrients (silicate, phosphate, and nitrate). Suitable habitat for L. pertusa was predicted using ENFA on a global and a regional scale that incorporated the north-east Atlantic Ocean. Regional prediction was reliable due to numerous presence points throughout the area, whereas global prediction was less reliable due to the paucity of presence data outside of the north-east Atlantic. However, the species niche was supported at each spatial scale. Predicted maps at the global scale reinforced the general consensus that the North Atlantic Ocean is a key region in the worldwide distribution of L. pertusa. Predictive modelling is an approach that can be applied to cold-water coral species to locate areas of suitable habitat for further study. It may also prove a useful tool to assist spatial planning of offshore marine protected areas. However, issues with eco-geographical datasets, including their coarse resolution and limited geographical coverage, currently restrict the scope of this approach.     

长山群岛海洋渔业资源的平均营养级变化特征

根据长山群岛 1965-2016 年渔业统计资料,分析长山群岛海域主要捕捞渔获物产量、平均营养级 （Mean trophic level, MTL）、渔业均衡指数 （Fishing in balance index,FiB） 年际变化,探讨其海洋渔业资源利用状况,并利用小波分析方法研究52年来渔获物 MTL 周期变化特征。研究表明： （1） 长山群岛捕捞产量、MTL 和 FiB 指数呈阶段性变化; （2） 长山群岛渔业资源开发经历初期开发、扩张捕捞、过度捕捞、资源破坏等四个阶段,渔业资源环境正在逐渐恶化;（3） 受人类捕捞活动影响,MTL 在 15~19 年和 24~34 年两种时间尺度下呈周期波动,30 年为第一主周期,17 年为第二主周期。长山群岛渔业资源破坏日益严重,未来几年平均营养级将呈下降趋势。为防止渔业资源进一步衰退,应加强捕捞活动管理力度,落实海洋渔业资源保护制度;完善预警机制,构建海洋渔业资源监测系统;同时应积极调整长山群岛渔业产业结构,提高资源产出效率.     

Effects of wave directionality on extreme response for a long end-anchored floating bridge

Reliable design codes are of great importance when constructing new civil engineering concepts such as floating bridges. Previously only a scarce number of floating bridges have been built in rough wave conditions and only limited knowledge of the extreme environmental conditions and the associated extreme response exists. To form a better design basis an increased understanding of the sensitivity in the structural response towards changes in short-crested sea parameters is needed. Furthermore, acquiring the necessary accuracy in simulated extreme response is often a computationally expensive endeavour and the number of simulations needed is often based on experience. The present study investigates the wave-induced short-term extreme response of a simplified end-anchored floating bridge concept for several wave environments with a return period of 100 years. The study includes convergence of the coefficient of variation for the extreme response for different realization lengths as well as number of realizations. The sensitivity in the structural response towards different main wave directions and spreading exponents is investigated and includes both transverse and vertical displacement response spectra and extreme Von Mises stress in the bridge girder cross-section. The extreme response is based on an accuracy of 2% in the coefficient of variation equivalent to 40 3-h realizations and a low sensitivity in the response is found for natural occurring spreading exponents and for main wave directions within 15° from beam sea.     

Fishing the last frontier: The introduction of the marine aquarium trade and its impact on local fishing communities in Papua New Guinea

Papua New Guinea has sometimes been called the world's last frontier for relatively undamaged coral reefs and their resources. In 2007, the country joined its neighbours in the marine aquarium trade. By licensing a private company, Papua New Guinea tried an alternative approach to the introduction of this activity. Under the so-called SeaSmart Programme, over 100 fishers were trained in sustainable collection techniques and handling of organisms, until the programme was shut down in 2010, and replaced by its successor, EcoAquariums PNG. This article contains the first study on the introduction of the marine aquarium trade into Papua New Guinea. It evaluates the overall outcomes of the SeaSmart Programme, lists targeted species, and their prices. The main focus is on the local collectors, and on the impacts of this new activity on them and their communities. Benefits from collecting marine ornamentals are assessed and their contribution to household income is quantified. Perceptions on non-financial benefits of the SeaSmart Programme are also gathered. The empirical work was carried out between September 2010 and February 2011 in the Central Province of Papua New Guinea. A total of 199 fishers including 44 aquarium fishers were interviewed in eight different communities in the Central Province of Papua New Guinea. The results show that marine aquarium fishing does provide benefits to local resource collectors, but also that this “sustainable” way of collecting of marine ornamentals might lead to some depletion.