蛤蜊科3种贝类16SrRNA基因片段及ITS2核苷酸序列分析

利用PCR技术分别扩增连云港及启东沿海蛤蜊科的西施舌（Coelomactra antiquata）、中国蛤蜊（Mactra chinensis）和四角蛤蜊（Mactra veneriformis）3种双壳贝的16SrRNA基因片段和ITS2核苷酸序列．测序后用DNAstar软件分析了核苷酸差异。结果显示：三种贝类16SrRNA基因片段长度相同，均为306bp（去除引物）．核苷酸存在多态性。共有45个变异位点，54个核苷酸发生了变异。全部为碱基置换。西施舌与中国蛤蜊此片段核苷酸的同源性为88．9％．与四角蛤蜊的同源性为88．6％．中国蛤蜊与四角蛤蜊的同源性为90．6％。三种蛤蜊ITS2序列分别为390bp（西施舌）、441bp（四角蛤蜊）和466bp（中国蛤蜊）。存在长度多态性．ITS2核苷酸差异分析结果显示．西施舌与中国蛤蜊的同源性为70．9％-71．1％，西施舌与四角蛤蜊的为70．5％-71．0％。中国蛤蜊与四角蛤蜊的同源性为88．1％-88．8％。ITS2序列分析结果与16SrRNA基因片段分析结果一致．2种分子分析法均显示中国蛤蜊与四角蛤蜊的亲缘关系近。     

The possible response of life zones in China under global climate change

The response of natural vegetation to climate change is of global concern. In this research, an aggregated Holdridge Life Zone System was used to study the possible response of life zones in China under doubled atmospheric CO₂ concentration with the input climatic parameters at 0.5×0.5° resolution of longitude and latitude from NCAR regional climate model 2 (RegCM2) coupled with the CSIRO global climate model. The results indicate that the latitudinal distribution of life zones would become irregular because of the complicated climate change. In particular, new life zones, such as subtropical desert (SD), tropical desert (TDE) and tropical thorn woodland (TTW), would appear. Subtropical evergreen broadleaved forest (SEBF), tropical rainforest and monsoon forest (TRF), SD, TDE and TTW zones would appear in the northeastern China. Cool-temperate mixed coniferous and broadleaved forest (CMC) and warm-temperate deciduous broadleaved forest (WDBF) zones would appear at latitudes 25–35°N. The temperate desert (TD) in the western China would become Tibetan high-cold plateau (THP), SEBF, WDBF and temperate steppe (TS), and a large part of THP would be replaced by TRF, TDE, SEBF, TS and TTW. The relative area (distribution area/total terrestrial area) of CMC, TRF, TDE and TTW zone would increase about 3%, 21%, 3% and 6%, respectively. However, the relative area of SEBF, TS, TD and THP would decrease about 5%, 3%, 19% and 4%, respectively. In all, the relative area of forests (CCF, CMC, WDBF, SEBF, TRF) would increase about 15%, but the relative area of desert (TD, SD, TDE, and TTW) and THP would decrease about 9% and 4%, respectively. Therefore, responses of different life zones in China to climate change would be dramatic, and nationwide corridors should be considered for the conservation of migrating species under climate change.     

Virtual Huanghe River System： Framework and Technology

1 Introduction Huanghe (Yellow) River basin is located in 32°–42°N, 96°–119°E. The area of the catchment is more than 752,000km2. The river is 5464km long with a drop in elevation of 4830m. Among the whole area, the moun- tainous and stone area accounts for 29%, loess and hills area 46%, sandy area 11% and plain area 14%, respec- tively. Different natural landscapes exist in this area. The Huanghe River flows through the Loess Plateau, where the soil is eroded seriously (Wang, 2002;…     

The HNCO ring in the Sgr B2 region

The large-scale structure associated with the 2′N HNCO peak in Sgr B2 [Minh, Y.C., Haikala, L., Hjalmarson, Å., Irvine, W.M., 1998. ApJ 498, 261 (Paper I)] has been investigated. A ring-like morphology of the HNCO emission has been found; this structure may be colliding with the Principal Cloud of Sgr B2. This “HNCO Ring” appears to be centered at (l,b) = (0.7°,−0.07°), with a radius of 5 pc and a total mass of 1.0 × 10⁵ to 1.6 × 10⁶ M. The expansion velocity of the Ring is estimated to be 30–40 km s⁻¹, which gives an expansion time scale of 1.5 × 10⁵ year. The morphology suggests that collision between the Ring and the Principal Cloud may be triggering the massive star formation in the Sgr B2 cloud sequentially, with the latest star formation taking place at the 2′N position. The chemistry related to HNCO is not certain yet, but if it forms mainly via reaction with the evaporated OCN⁻ from icy grain mantles, the observed enhancement of the HNCO abundance can be understood as resulting from shocks associated with the collision between the Principal Cloud and the expanding HNCO Ring.     

X-ray spectral evolution of low-mass X-ray binary GX 349+2

We present the results of a systematic investigation of spectral evolution in the Z source GX 349+2, using data obtained during 1998 with the Proportional Counter Array (PCA) on-board the RXTE satellite. The source traced a extended normal branch (NB) and flaring branch (FB) in the colour–colour diagram (CD) and the hardness-intensity diagram (HID) during these observations. The spectra at different positions of the Z-track were best fitted by a model consisting of a disc blackbody and a Comptonized spectrum. A broad (Gaussian) iron line at ∼6.7 keV is also required to improve the fit. The spectral parameters showed a systematic and significant variation with the position along the Z-track. The evolution in spectral parameters is discussed in view of the increasing mass accretion rate scenario, proposed to explain the motion of Z sources in the CD and the HID.     

Characters of the Pco2 and CO2 Flux in the East China Sea in Autumn

Pco2 of air and seawater samples from the East China Sea(ECS) were measured in situ in autumn, 1994,Ocean currents,terrestrial fluviation,biological activities,etc.,Pco2 char-acters in air and seawater were investigated,CO2 flux and its character in the East China Sea are discussed on the basis of the Pco2 profiles of air and seawater,It was clear that the nearshore was the source of CO2;and tht the oulter sea area was the sink of CO2; and that the shelf area of the EXS is a net sink for atmospheric CO2 in autumn.     

X-ray synchrotron emission from the oblique shock in the jet of the powerful radio galaxy 3C 346

We report the first detection, with Chandra , of X-ray emission from the jet of the powerful narrow-line radio galaxy 3C 346. X-rays are detected from the bright radio and optical knot at which the jet apparently bends by approximately 70°. The Chandra observation also reveals a bright galaxy-scale atmosphere within the previously known cluster and provides a good X-ray spectrum for the bright core of 3C 346. The X-ray emission from the knot is synchrotron radiation, as seen in lower-power sources. In common with these sources, there is evidence of morphological differences between the radio/optical and X-ray structures, and the spectrum is inconsistent with a one-component continuous-injection model. We suggest that the X-ray-bright knot is associated with a strong oblique shock in a moderately relativistic, light jet, at ∼ 20° to the line of sight, and that this shock is caused by the jet interacting with the wake in the cluster medium behind the companion galaxy of 3C 346. The general jet curvature can result from pressure gradients in the cluster atmosphere.     

A Chandra detection of the radio hotspot of 3C 123

Chandra X-ray Observatory observations of the powerful, peculiar radio galaxy 3C 123 have resulted in an X-ray detection of the bright eastern hotspot, with a 1-keV flux density of ∼5 nJy. The X-ray flux and spectrum of the hotspot are consistent with the X-rays being inverse-Compton scattering of radio synchrotron photons by the population of electrons responsible for the radio emission ('synchrotron self-Compton emission') if the magnetic fields in the hotspot are close to their equipartition values. 3C 123 is thus the third radio galaxy to show X-ray emission from a hotspot which is consistent with being in equipartition. Chandra also detects emission from a moderately rich cluster surrounding 3C 123, with L _X(2–10 keV)=2×10⁴⁴ erg s⁻¹ and kT ∼5 keV, and absorbed emission from the active nucleus, with an inferred intrinsic column density of 1.7×10²² cm⁻² and an intrinsic 2–10 keV luminosity of 10⁴⁴ erg s⁻¹.     

量子化学方法研究氯代苯酚类化合物对水生物毒性的构效关系

对20个氯代苯酚类化合物进行了CNDO/2量子化学计算,讨论了化合物电子结构与其对水生物翻车鱼(Bluegill)、花鳉鱼(Guppy)、和虾(Shrimp)半致死量之间的关系,分别获得了表示其构效关系的三个线性方程,它们的显著性均远高于α=0.01水平。结果表明:氯代苯酚化合物苯环上碳原子的兀电荷之和(∑Qπ_R)越大苯环上碳原子的净电荷之和(∑Q_R)越大,或LUMO轨道能(E_(LUMO))越低,均使化合物对Bluegill和Guppy水生物的毒性增大;而化合物苯环上羟基邻位的碳原子的兀电荷布居(P_3)越大,同时HOMO轨道能E_(HOMO)越高,则化合物对Shrimp毒性也越大。据此,可预测氯代苯酚类化合物对上述三种水生物的毒性。     

脱水对浒苔光合作用的影响

对汕头南澳岛潮间带海藻浒苔(Enteromorpha