地下水中钙和镁的离子色谱法同时测定

改进了离子色谱分析地下水中钙和镁的方法。以IonPac CS12A为分离柱,稀盐酸为淋洗液,电导检测器检测,对地下水中的锂、钾、钠、钙和镁进行同时测定。方法具有较宽的线性范围和较高的灵敏度,钙、镁的浓度分别在0～500mg/L和0～250mg/L内呈良好的线性关系;钙的检出限为1.50μg/L,镁的检出限为0.89μg/L。对不同浓度钙、镁水质标准样品进行分析测定,同时对实际样品进行不同稀释倍数分析验证,方法精密度(RSD,n=8)为0.19%～1.89%,无显著的基体效应影响。方法可满足全国地下水调查评价规范要求,适于地下水样品中锂、钾、钠、钙和镁离子的同时测定。     

利用氮氧同位素研究桂林寨底地下河硝酸盐来源

工农业生产的迅速发展使地下水硝酸盐氮(NO3-—N)污染成为世界性的环境问题。地下水中硝酸盐的来源研究在水文地质结构特殊的西南岩溶地区就显得尤为重要。稳定氮同位素和氧同位素在地下水硝酸盐的来源示踪研究中有着广泛的应用。本研究选取岩溶地区典型地下河——广西桂林寨底地下河为研究对象,通过氮氧同位素数据,判断寨底地下河硝酸盐来源是以动物粪便为主的农家肥,为该区地下水的保护和利用提供科学依据。     

电气石矿物吸附铜离子试验研究

本文研究了电气石的单晶、电气石微粉对Cu2＋的吸附作用,试验表明：电气石单晶体表面存在着以C轴为两极的静电场,该静电场对Cu2＋的吸附作用效果明显。制备的电气石微粒越细,吸附效果越好。电气石的电场效应使得它在治理污水中Cu2＋污染具有广阔的前景。     

高亚硝酸盐胁迫下日本囊对虾肝胰腺的转录组分析

海岸带盐度过渡区域水体中氮、磷的准确测定对陆海统筹及海岸带管理起着至关重要的作用。为探究盐度对氮、磷测定准确度的影响,进而明确河口海岸带淡水-咸水盐度过渡区不同形式氮、磷测定方法的选择依据,分别采用淡水和海水的相关国家标准方法,测定不同盐度下不同质量浓度氮、磷的人工海水和天然海水水样中氨氮、亚硝氮、总氮、活性磷酸盐和总磷的质量浓度。结果表明,盐度对某些氮、磷的淡水法和海水法测定结果有一定程度的影响。对氨氮的测定,当S≤4.0时淡水法或海水法均可准确测定水体中的氨氮,S>4.0时应对水样进行pH 调整预处理后用海水法进行测定;对亚硝氮的测定,淡水法和海水法的测定结果无明显差异;对总氮的测定,当S≤5.0时应采用操作简单、测定准确的淡水法测定,S>5.0时应采用海水法。对水体中的活性磷酸盐的测定,在低盐低磷(S≤35.0且ρPO3-4 ≤0.2mg·L-1)的情况下盐度对淡水法和海水法测定结果的影响微乎其微,而对于高盐高磷(S>35.0且ρPO3-4 > 0.2mg·L-1)的水样选择灵敏度更高的海水法测定更准确;对总磷的测定,选取海水法测定准确度更高。研究结果为海岸带水体不同形式氮、磷质量浓度的准确测定与方法选择提供了科学依据。     

Uptake and recovery of soil nitrogen by bryophytes and vascular plants in an alpine meadow

Due to their particular physiology and life history traits, bryophytes are critical in regulating biogeochemical cycles and functions in alpine ecosystem. Hence, it is crucial to investigate their nutrient utilization strategies in comparison with vascular plants and understand their responses to the variation of growing season caused by climate change. Firstly, this study testified whether or not bryophytes can absorb nitrogen(N) directly from soil through spiking three chemical forms of 15N stable isotope tracer. Secondly, with stronger ability of carbohydrates assimilation and photosynthesis, it is supposed that N utilization efficiency of vascular plants is significantly higher than that of bryophytes. However, the recovery of soil N by bryophytes can still compete with vascular plants due to their greater phytomass. Thirdly, resource acquisition may be varied from the change of growing season, during which N pulse can be manipulated with 15N tracer addition at different time. Both of bryophytes and vascular plants contain more N in a longer growing season, and prefer inorganic over organic N. Bryophytes assimilate more NH4+ than NO3– and amino acid, which can be indicated from the greater shoot excess 15N of bryophytes. However, vascular plants prefer to absorb NO3– for their developed root systems and vascular tissue. Concerning the uptake of three forms N by bryophytes, there is significant difference between two manipulated lengths of growing season. Furthermore, the capacity of bryophytes to tolerate N-pollution may be lower than currently appreciated, which indicates the effect of climate change on asynchronous variation of soil N pools with plant requirements.     

低序级断层成像技术在c13地区的应用

低序级断层在地震剖面上,通常表现为具有微小错动,同相轴扭曲,或者振幅变弱,延伸短、断距小、识别难,描述难等特征。在对采集处理过程中影响低序级断层识别的观测系统面元网格、信噪比、频率、速度误差等因素详细分析的基础上,通过建立全波场正演地质模型,借助方查体属性技术以及井资料,研究了针对性的处理技术。同时利用相干体属性、曲率属性沿层切片、方差体等技术进行有效识别低级序断层,取得了较好的效果。     

重金属胁迫对两种海洋饵料微藻的急性毒性效应研究

在模拟生态环境条件下研究了铜(Cu2+)、镉(Cd2+)和锌(Zn2+)3种重金属离子对2种海洋饵料微藻(青岛大扁藻(Platymonas helgolandica)和等鞭金藻8701(Isochrysis galbana 8701))的急性毒性效应,分析其对种群增长的影响,并利用透射电镜对微藻亚显微结构的变化进行分析。在实验浓度范围内,2种微藻的种群增长对3种重金属离子的急性胁迫表现出类似的变化趋势,随着胁迫浓度的增加,微藻的相对增长率均呈现出不断下降的趋势;通过比较2种微藻的48hEC50和96h-EC50发现:3种重金属离子的毒性程度为Cu2+Cd2+Zn2+,等鞭金藻8701对重金属胁迫的敏感性高于青岛大扁藻。2种微藻的亚显微结构观察显示,二者的膜结构及细胞核对Cu2+胁迫并不太敏感;与对照组相比等鞭金藻8701的色素体及贮能物质受影响较严重,而青岛大扁藻的叶绿体及淀粉粒受影响较大。     

一株海洋微藻的分离鉴定及其产油条件优化

自青岛近岸海水中分离到一株硅藻,经形态学和分子生物学鉴定,表明该藻与小头菱形藻(Nitzschia microcephala)最为相近,18SrDNA相似性99%以上。研究以f/2培养基为基础,对该藻的生长和油脂积累条件进行探析,表明其在pH=6~8时具有较高的生长速率,最大可达到0.01d-1,油脂含量随pH的升高而降低;添加SiO2-3可以抑制菱形藻的生长和油脂积累;高浓度的Fe3+能促进菱形藻的生长;在Zn2+浓度为1.74μmol/L的条件下,菱形藻能够较好地生长并且进行油脂的积累;其中pH、SiO2-3和Fe3+是影响该藻生长和油脂积累的主要因子。正交试验研究表明,该藻在f/2培养液中生长和油脂积累的最优条件为初始pH=9,SiO2-3和Fe3+添加浓度分别为0.07和100μmol/L。在该最优条件下,该藻的生物量和油脂积累可分别达到94.7mg/L和19.6%。     

Measurement of trace nitrate concentrations in seawater by ion chromatography with valve switching

An ion chromatographic method with a valve switching facility was developed to determine trace nitrate concentrations in seawater using two pumps, two different suppressors, and two columns. A carbohydrate membrane desalter was used to reduce the high concentrations of sodium salts in samples. In this method, trace nitrate was eluted from the concentrator column to the analytical columns, while the matrix flowed to waste. Neither chemical pre-treatment nor sample dilution was required. In the optimized separation conditions, the method showed good linearity （R〉0,99） in the 0.05 and 50 mg/L concentration range, and satisfactory repeatability （RSD〈5%, n=6）. The limit of detection for nitrate was 0.02 mg/L. Results showed that the valve switching system was suitable and practical for the determination of trace nitrate in seawater.     

Trophic ecology of Pomatoschistus microps within an intertidal bay (Roscoff,France), investigated through gut content and stable isotope analyses

The diet of Pomatoschistus microps has been studied using both gut content and stable isotope analyses. In the Roscoff Aber Bay (Brittany, France), this fish is commonly found on sandy muddy intertidal flats. Gut content analyses were also interpreted using trophic indices. Owing to the large diversity of prey consumed, these indices emphasised the opportunistic feeding behaviour of P. microps. Here, this species fed mainly on endofauna with meiofauna being of high relative importance. The main biotic components of its trophic habitat, characterized by δ¹³C and δ¹⁵N, provided evidence of a major trophic pathway based on drift Enteromorpha sp. Trophic positions estimated by both diet analyses and isotopic analyses led to similar results. In this bay, P. microps is a first‐order predator with a low degree of omnivory. Despite a preferential consumption of the amphipod Corophium arenarium, we assumed that this goby behaves as a generalist feeding on a uniform variety of endofauna taxa.