海带CRY-DASH基因的克隆与转录表达分析

利用cDNA末端快速克隆(RACE)技术,获得海带(Saccharina japonica) CRY-DASH基因(SjCRYDASH)全长序列,结构分析发现,其ORF区长1779 bp,编码592个氨基酸。进行氨基酸同源序列比对,其与其他藻类和高等植物间存在两个重要辅基MTHF和FAD结合的保守域。通过不同光质照射诱导海带幼孢子体,发现蓝光、白光诱导1h后,均能使SjCRY-DASH转录水平上升,且SjCRY-DASH对蓝光的响应更强烈。本研究结果为研究大型褐藻-海带CRY-DASH受光诱导调控功能打下基础。     

Diversity and coverage of seagrass ecosystems in south-west Madagascar

Seagrass meadows provide important nursery and feeding grounds for many commercially valuable fish species. Here, we address the paucity of published information on the status of seagrasses in Madagascar by documenting the results from ecological surveys of 11 seagrass beds in Velondriake, a locally managed marine area (LMMA) in south-west Madagascar. The diversity and coverage of meadows was highest in the north of the LMMA with up to 51% coverage, and lowest in the south (26%). Overall, eight seagrass species were recorded: Cymodocea rotundata, C. serrulata, Halodule uninervis, Halophila ovalis, H. stipulacea, Syringodium isoetifolium, Thalassia hemprichii and Thalassodendron ciliatum. We discuss the natural and anthropogenic factors that may account for the observed low diversity of seagrasses in southern Velondriake, including overfishing, beach-seining, cyclones, siltation and mangrove deforestation. Based on these baseline surveys, as well as discussions with local communities, it is recommended that measures should be taken to reinforce efforts to ban beach-seines and that the role of seagrasses as carbon sinks and potential sustainable financing options through blue carbon initiatives should be investigated through further, more detailed surveys.     

Possible solution to the problem of the extreme mass ratio W UMa-type binaries

When the total angular momentum of a binary system is at a critical (minimum) value, a tidal instability occurs (Darwin's instability), eventually forcing the stars to merge into a single, rapidly rotating object. The instability sets in at some critical separation which in the case of contact binaries corresponds to a minimum mass ratio depending on dimensionless gyration radius k ₁. If one considers   n = 3  polytrope (fully radiative primary with  Γ₁= 4/3  ),   k ²₁= 0.075  and   q _min≈ 0.085–0.095  . There appears to be, however, some W UMa-type binaries with q values very close, if not below these theoretical limits, implying that primary in these systems is probably more centrally condensed. We try to solve the discrepancy between theory and observations by considering rotating polytropes. We show by deriving and solving a modified Lane–Emden equation for   n = 3  polytrope that including the effects of rotation does increase the central concentration and could reduce   q _min  to as low as 0.070–0.074, more consistent with the observed population.     

Temporal and spatial changes of blue water and green water in the Taihang Mountain Region,China, in the past 60 years

ABSTRACT

Appropriate allocation of limited freshwater resources to humans and ecosystems is an important issue hampering sustainable development in mountainous regions. The Taihang Mountain Region (TMR), including the Yellow and Hai river basins, is an important water source area for the North China Plain. The distributed hydrological model Water and Energy transfer Processes in Large river basins (WEP-L) was used to simulate the water cycle processes and to summarize the temporal and spatial changes in the blue and green water in the TMR from 1956 to 2015. The results show that in the period 2011–2015 the annual average blue water decreased by 7.31 × 10⁹ m³, while the annual average green water increased by 13.60 × 10⁹ m³ compared to 1956–1960. At the inter-annual time scale, the blue water exhibited a downward trend while the green water exhibited an upward trend. The amount of seasonal blue water in the TMR is ranked in descending order: summer, autumn, spring and winter, while for green water, the rank is summer, spring, autumn and winter. The amounts of blue and green water are higher on the windward than on the leeward slopes. The blue water yield is generally higher in forests and grasslands than in farmland, while the green water exhibits the opposite response. A greater emphasis should be placed on the widening gap between blue water and green water due to climate warming, and on soil and water conservation measures.     

Direct N-body modelling of stellar populations: blue stragglers in M67

We present a state-of-the-art N -body code which includes a detailed treatment of stellar and binary evolution as well as the cluster dynamics. This code is ideal for investigating all aspects relating to the evolution of star clusters and their stellar populations. It is applicable to open and globular clusters of any age. We use the N -body code to model the blue straggler population of the old open cluster M67. Preliminary calculations with our binary population synthesis code show that binary evolution alone cannot explain the observed numbers or properties of the blue stragglers. On the other hand, our N -body model of M67 generates the required number of blue stragglers and provides formation paths for all the various types found in M67. This demonstrates the effectiveness of the cluster environment in modifying the nature of the stars it contains, and highlights the importance of combining dynamics with stellar evolution. We also perform a series of N =10 000 simulations in order to quantify the rate of escape of stars from a cluster subject to the Galactic tidal field.     

Effects of different light conditions on repair of UV-B-induced damage in carpospores of Chondrus ocellatus Holm

We evaluated the effects of ultraviolet-B(UV-B) radiation and different light conditions on the repair of UV-B-induced damage in carpospores of C hondrus ocellatus Holm(Rhodophyta) in laboratory experiments. Carpospores were treated daily with different doses of UV-B radiation for 48 days,when vertical branches had formed in all treatments; after each daily treatment,the carpospores were subjected to photosynthetically active radiation(PAR),darkness,red light,or blue light during a 2-h repair stage. Carpospore diameters were measured every 4 days. We measured the growth and cellular contents of cyclobutane pyrimidine dimers(CPDs),chlorophyll a,phycoerythrin,and UV-B-absorbing mycosporine-like amino acids(MAAs) in carpospores on Day 48. Low doses of UV-B radiation(36 and 72 J/m 2) accelerated the growth of C. ocellatus. However,as the amount of UV-B radiation increased,the growth rate decreased and morphological changes occurred. UV-B radiation significant damaged DNA and photosynthetic pigments and induced three kind of MAAs,palythine,asterina-330,and shinorine. PAR conditions were best for repairing UV-B-induced damage. Darkness promoted the activity of the DNA darkrepair mechanism. Red light enhanced phycoerythrin synthesis but inhibited light repair of DNA. Although blue light,increased the activity of DNA photolyase,greatly improving remediation efficiency,the growth and development of C. ocellatus carpospores were slower than in other light treatments.     

中国农业广义水资源利用系数及时空格局分析

为统一核算区域蓝水、绿水资源有效利用程度,构建了农业广义水资源利用系数（E_g）指标,核算中国31个省区粮食生产中的E_g,并基于空间自相关和通径分析方法对其时空格局及成因进行分析。中国1998-2010年E_g为0.588,大部分省区呈随时间增大趋势;E_g相似的省区在空间上显著地聚集,高值省区聚集于西部,低值省区集中于东南地区,且空间聚集现象呈减弱趋势;农业生产管理水平对E_g的影响大于气候要素,社会经济条件影响最小。结合广义水资源利用状况,西南省区可扩大粮食生产规模以发挥E_g较大优势,东北与华北粮食主产区应通过提高E_g以保障粮食安全和水资源可持续利用。研究可发展农业用水效率评价理论同时为国家粮食生产与水资源管理策略的制定提供参考。     

Adsorption capacity and mineralogical and physico-chemical characteristics of Shuwaymiyah palygorskite (Oman)

In this paper, Shuwaymiyah palygorskite in the Sultanate of Oman has been characterized mineralogically by X-ray diffraction (XRD) and electron microscopy, chemically by oxide compositions, structural formulae, and cation exchange capacity (CEC), and physically by specific surface area and adsorption isotherms. Batch adsorption studies were performed to evaluate the adsorption performance of methylene blue (MB) basic dye on the local clay mineral. The quantitative XRD analysis indicates that the purity of some selected samples of palygorskite clay is very high (about 70% of the clay minerals are palygorskite and 30% kaolinite). The scanning electron microscopy (SEM) and transmission electron microscopy (TEM) images clearly support this conclusion. The adsorption equilibrium revealed that Shuwaymiyah palygorskite clay can uptake up to 51 mg of MB per 1 g mass of clay. MB adsorption is best fitted by Langmuir isotherm, and a pseudo-second-order kinetic model can be efficiently used to predict the kinetic of adsorption of MB by the palygorskite. The results obtained from these laboratory-scale adsorption tests indicate the promising adsorption capability of the Omani palygorskite.     

Collisions and close encounters involving massive main-sequence stars

We study close encounters involving massive main-sequence stars and the evolution of the exotic products of these encounters as common-envelope systems or possible hypernova progenitors. We show that parabolic encounters between low- and high-mass stars and between two high-mass stars with small periastrons result in mergers on time-scales of a few tens of stellar free-fall times (a few tens of hours). We show that such mergers of unevolved low-mass stars with evolved high-mass stars result in little mass-loss  (∼0.01 M_⊙)  and can deliver sufficient fresh hydrogen to the core of the collision product to allow the collision product to burn for several million years. We find that grazing encounters enter a common-envelope phase which may expel the envelope of the merger product. The deposition of energy in the envelopes of our merger products causes them to swell by factors of ∼100. If these remnants exist in very densely populated environments  ( n ≳ 10⁷ pc⁻³)  , they will suffer further collisions which may drive off their envelopes, leaving behind hard binaries. We show that the products of collisions have cores rotating sufficiently rapidly to make them candidate hypernova/gamma-ray burst progenitors and that ∼0.1 per cent of massive stars may suffer collisions, sufficient for such events to contribute significantly to the observed rates of hypernovae and gamma-ray bursts.     

Laboratory studies on zooplankton‐cyanobacteria interactions

Laboratory studies on cyanobacteria‐zooplankton interactions have largely focused on the inadequacy of cyanobacteria as a food source. Some features of cyanobacteria can be regarded as anti‐herbivore defences. Large colonies of Aphanizomenon, Anabaena and Microcystis cannot be handled by zooplankton, but do not interfere seriously with the filtering process. Small colonies and filaments, however, may cause severe inhibition of the feeding process by mechanical interference. This reduces zooplankton growth, reproduction, and survival. Copepods, rotifers, and Bosmina are less affected by mechanical disturbance than cladocerans. If ingested, some cyanobacteria may be poorly digested or may not provide essential nutrients. Some cyanobacteria are reported to be toxic to zooplankton. Several strains of Microcystis produce an endotoxin, but the amount of toxin produced differs among strains and with the condition of Microcystis. Zooplankton encountering toxic cells cease feeding. Some evidence of an extra‐cellular herbivore deterrent was found for Anabaena. Thus, cyanobacteria may either be individually protected or may create an environment that is unfavourable for efficient grazers (e.g., Daphnia).