刺参(Apostichopus japonicus)腐皮综合症发生相关蛋白的分离与鉴定

采用比较蛋白质组学的方法,对2011年10月20日取自辽宁省普兰店室内养殖厂腐皮综合症刺参和健康个体肠组织中差异表达蛋白进行了研究。结果表明,在优化的电泳条件(80μg脱盐蛋白、pH4—7[NL,13cm]的IPG预制胶条)下,共检测到约700个蛋白质点;表达量差异两倍以上的蛋白点51个,最大表达差异比率为4.32;对其中重复性一致的30个差异点进行质谱鉴定,成功鉴定了23个差异蛋白,包括铁蛋白,过氧化氢酶,泛素相关修饰子3,细胞色素氧化酶和肌动蛋白等;进一步扫描实验室完成的刺参转录组数据,筛选了过氧化氢酶和泛素相关修饰子3的EST序列。本研究是蛋白层面上刺参腐皮综合症发生机制的有效尝试,研究结果为进一步认识该疾病的发生机制提供了参考。     

Phylogeography of the shrimp Palaemon floridanus (Crustacea: Caridea: Palaemonidae): a partial test of meta‐population genetic structure in the wider Caribbean

Marine organisms with a pelagic stage are often assumed to display minor population structure given their extended larval development and subsequent high long‐distance dispersal ability. Nonetheless, considerable population structure might still occur in species with high dispersal ability due to current oceanographic and/or historical processes. Specifically, for the wider Caribbean and Gulf of Mexico, theoretical and empirical considerations suggest that populations inhabiting each of the following areas should be genetically distinct: Panama, Belize, Southwest Florida (Tampa), and Southeast Florida (Fort Pierce). This study tests the hypothesis of significant genetic differentiation in Palaemon floridanus populations across the wider Caribbean and Gulf of Mexico. Population level comparisons were conducted using sequences of the mtDNA COI. In agreement with predictions, AMOVA and pairwise F_ST values demonstrated population differentiation among most pairs of the studied populations. Only Panama and East Florida populations were genetically similar. An isolation‐with‐migration population divergence model (implemented in IMA2) indicated that population divergence with incomplete lineage sorting can be invoked as the single mechanism explaining genetic dissimilarity between populations from the east and west coast of Florida. Historical demographic analyses indicated demographic expansion of P. floridanus in some localities [recent in Panama and ancient in East Florida and the wider Caribbean (entire dataset)] but constant population in other localities (in Belize and West Florida). This study rejects the idea of panmixia in marine species with high long‐distance dispersal ability. Contemporary and historical processes might interact in a complex manner to determine current phylogeographic patterns.     

CO2加富培养对鼠尾藻克制赤潮异弯藻效果的影响

采用实验生态学的方法,研究了大型海藻鼠尾藻(Sargassum thunbergii)对赤潮微藻中肋骨条藻(Skeletonema costatum)的克生效应及其对 CO2加富的响应变化.结果表明,正常培养的鼠尾藻新鲜组织、干粉末和水溶性抽提液能够导致中肋骨条藻的细胞密度显著降低(P<0.05), CO2加富培养的鼠尾藻新鲜组织、干粉末和水溶性抽提液能够更加明显地抑制中肋骨条藻种群生长(P<0.01).实验结果证实鼠尾藻对中肋骨条藻具有克生效应,而 CO2加富培养引起了鼠尾藻对中肋骨条藻克生效应的变化,使其对中肋骨条藻生长的抑制作用显著加强.正常培养的鼠尾藻培养水过滤液能够显著抑制中肋骨条藻的生长(P<0.05),而 CO2加富培养的鼠尾藻培养水过滤液对中肋骨条藻的生长没有表现出明显的影响(P>0.05),因此推测 CO2加富培养可能改变了鼠尾藻对中肋骨条藻克生作用的方式,作用方式由分泌克生物质转变为细胞直接接触转递.     

泡沫镍基P-25薄膜光催化降解水中喹啉

为了提高TiO²薄膜的光催化效率和使用稳定性,采用浸渍涂布法将粉体P-25 TiO₂负载在泡沫镍基片上,制备泡沫镍基负载P-25薄膜光催化材料,利用XPS和FE-SEM分析了薄膜的晶相和形貌,并考察了不同条件下P-25薄膜对水中喹啉的降解效能。研究发现：P-25薄膜均匀覆盖泡沫镍基片表面,薄膜呈混晶结构,表面呈现微裂痕。实验表明：增大光照强度和曝气量能够明显提高水中喹啉的光催化降解率;低pH有利于水中喹啉的去除;初始浓度和温度不是光催化反应的主控因子;曝气产生的吹脱、扰动和水力紊流效应对光催化反应的影响不明显。结果显示：不改变水溶液温度和pH条件下,P-25薄膜对水中喹啉有良好的去除效果,并可多次重复使用。     

深部咸水层CO2地质储存工程场地选址技术方法

CO₂地质储存作为环保型工程项目,其合理的工程场址是实现长期、安全封存CO₂的首要前提。我国CO₂地质储存工作刚刚起步,尚未形成成熟的选址技术方法体系。CO₂地质储存工程场地选址应遵循目标储层有效储存量大、安全、经济、符合一般建设项目环境保护选址条件、不受外部不良地质因素影响的原则,选址技术宜采用多尺度目标逼近法,选址程序包括规划选址和工程选址两大阶段。规划选址包括国家级、盆地级和目标区级潜力评价3个阶段;工程选址旨在通过目标靶区确定、综合地质调查、钻探及灌注试验和选定场地多因子排序综合评价,最终选出良好的工程场址。深部咸水层CO₂地质储存工程场地多尺度目标逼近选址技术方法对我国批量开展CO₂地质储存工程场地选址具有一定的指导意义。     

基于不等式约束的磁共振信号T2谱多指数分解法及算法改进

磁共振地下水探测信号的弛豫时间T₂和振幅与地下岩石孔隙大小和地下水的含水量直接相关。目前,磁共振地下水探测方法中,信号的衰减特性由平均弛豫时间T^*₂来描述。然而,对于复杂的多孔介质组成的岩石结构,仅由T^*₂无法准确描述地下孔隙情况。详细研究了基于不等式约束的多指数分解法,进行了信号T₂谱的提取,并重新计算多指数形式下的信号初始振幅。结果表明：对比信号在不同采集时间下的T₂谱提取结果,延长信号的采集时间有助于T₂谱的准确提取;通过磁共振仿真信号计算并分析不同信噪比下的T₂谱提取结果,信噪比大于30 dB时相对误差平均值小于5%;进行算法改进,得到信噪比大于10 dB时相对误差平均值小于5%的结果,且幅值和T₂的相对误差平均值降低了约1/3。由此可知:采用多指数分解法能够获得磁共振信号的T₂谱,正则化技术和加权修正能够提高T₂谱的准确度和抗干扰能力;与单指数方式相比,采用多指数拟合能够更准确地恢复信号初始振幅。     

中国二氧化碳排放数据比较分析

将世界主要相关研究机构和数据库（IEA、BP、EDGAR/PBL/JRC、CDIAC、EIA和CAIT）发布的中国二氧化碳排放数据与中国官方数据和估算进行比较,认为数据之间的差异来源于计算范围、方法和基础数据的不同,其中能源消费数据的差异是造成一些数据库估计值偏高的重要原因。研究认为IEA和CAIT数据与中国官方数据具有较好的可比性。建议中国加强煤炭统计工作,提高官方数据公布频率并提高清单完整性。     

Water quality evaluation in Tidal River reaches of Liaohe River Estuary, China using a revised QUAL2K model

Rivers in the Liaohe River Estuary area have been seriously polluted by discharges of wastewater containing petroleum pollutants and nutrients. In this paper, The Enhanced Stream Water Quality Model (QUAL2K) and its revised model as well as One-dimensional Tide Mean Model (1D model) were applied to predict and assess the water quality of the tidal river reach of the Liaohe River Estuary. Dissolved oxygen (DO), biochemical oxygen demand (BOD₅), ammonia nitrogen (NH₃-N) and total phosphorus (TP) were chosen as water quality indices in the two model simulations. The modelled results show that the major reasons for degraded rivers remain petroleum and non-point source pollution. Tidal water also has a critical effect on the variation of water quality. The sensitivity analysis identifies that flow rate, point load and diffuse load are the most sensitive parameters for the four water quality indices in the revised QUAL2K simulation. Uncertainty analysis based on a Monte Carlo simulation gives the probability distribution of the four water quality indices at two locations (6.50 km and 44.84 km from the river mouth). The statistical outcomes indicate that the observed data fall within the 90% confidence intervals at all sites measured, and show that the revised QUAL2K gives better results in simulating the water quality of a tidal river.     

卫星散射计与海面平台所测的风场数据比较

基于南海北部海面PY30-1石油平台气象站测风仪2011年7月19日—2012年9月17日实测的风场数据,分别开展了对卫星搭载的ASCAT和HY-2散射计所测风场数据的比较研究,分析散射计的测风能力(选取的时空窗口为30 min和25 km)。结果表明:在南海北部海域,ASCAT 散射计所测风速和PY30-1石油平台气象站观测风速的均方根误差为2.53 m/s,风向偏差较大,均方根误差为47.87°;HY-2散射计所测风速和PY30-1石油平台气象站观测风速的均方根误差为3.41 m/s,风向的均方根误差为58.66°。分别按低、中和高风速的不同条件将ASCAT和HY-2散射计所测的风场数据与PY30-1石油平台气象站观测的风场数据加以比较可知,ASCAT和HY-2散射计都具有较好的测风能力, 前者所测风速与PY30-1石油平台气象站测风仪观测风速的均方根误差稍小于后者。在150 min和15 km的时空窗口下,ASCAT与HY-2散射计所测风速的均方根误差为0.72 m/s,风向的均方根误差为8.50°。     

Gravity field and tectonic features of Block L2 in the Lamu basin,Kenya

A gravity survey on the scale of 1: 250 000 was carried out in Block L2 located in the Lamu basin of south‐east Kenya in order to study tectonic features and find out favourable petroleum prospects in the block. This paper, through data processing and synthetic interpretation of the measured gravity data in the block, discusses characteristics of the gravity field and their geological implications, determines the fault system and the basement depth, analyses features of the main strata, divides structure units and predicts favourable petroleum zones. In the block, the regional gravity anomaly is mainly caused by the inclined Moho surface that rises in the east and subsides in the west topographically and the Bouguer gravity anomaly primarily reflects the superimposition of the gravity effect derived from the Moho surface and the basement relief. Two groups of faults extending NW (NWW) and NE (NEE) respectively are dominant in the block and their activities resulted in the framework of east‐west zoning and south‐north blocking. The basement depth greatly changes in an alternative high and low pattern. The Permian‐Triassic, Jurassic and Tertiary strata are extensively developed, while the Cretaceous is only developed in the east of the block. Structurally, the block can be divided into five units, of which the Tana sag shows excellent source‐reservoir‐seal associations and is a favourable target for future petroleum exploration.