一种新方法——低渗诱导虾夷扇贝三倍体的研究

研究开发了1种多倍体诱导的新方法,采用低渗抑制受精卵第2极体(PB2)的释放诱导虾夷扇贝(Patinopecten yessoensis)三倍体.结果表明:以低渗盐度S=12,在第一个PB2出现时开始处理,持续处理20 min,诱导率可高达(84.74±3.82)%;三倍体处理组的幼虫比对照组具有明显的生长优势,但是幼虫存活率低于二倍体对照组.     

不同过滤条件下黄色物质的吸收光谱演化特征分析

在实验室对青岛近岸现场水样进行处理、分析,获得了原始状态下水样和被过滤（0.22μm,0.45μm）黄色物质水样的吸收光谱数据,除进一步验证了黄色物质光谱分布模式外,还测得黄色物质光吸收随滤膜孔径的变化。30个现场样品在波长380nm处吸收系数统计平均值^—Ay（380）分析表明,当使用0.45μm过滤器对水样进行处理,所获得的^—Ay（380）值要比未经过处理的现场水样所测得的^—Ay（380）值高约2.1％;而用0.22μm过滤器对现场水样进行处理,所获得的^—Ay（380）值要比未经过处理的现场样品所测得的^—Ay（380）值高约12．6％,要比用0．45μm过滤器处理的现场样品所测得的^—Ay（380）值高约10.3％,这些数据的获得为现有已获黄色物质数据的“归一化”和统一评判以及未来黄色物质测量数据的准确性判断与比较提供了参考。     

PVDF管式超滤膜在低渗油田水处理中的研究及应用——以江苏台兴油田为例

根据低渗油田和特低渗油田的特点,研制出PVDF管式超滤膜,开展室内基础研究,并在江苏台兴油田进行中试和大规模的现场试验。经过处理后,水质标准达到油田回注水A1标准（SY／T5329-1994）,满足了低渗油田和特低渗油田的注水要求。     

Effects of vegetation and sewage load on mangrove crab condition using experimental mesocosms

Constructed wetlands, especially mangroves, have been studied for their usefulness in sewage treatment but the effects of mangrove vegetation and a sewage load on mangrove macrofauna have been given little attention. Ocypodid crabs are important components of mangrove forests and constitute good bioindicators of the functioning of the ecosystem as a whole. In constructed mangrove mesocosms, three vegetation treatments (bare substratum, and Avicennia marina and Rhizophora mucronata seedlings) were subjected to 0, 20, 60 and 100% sewage loads from a nearby hotel. The physiological condition of introduced Uca annulipes and Uca inversa was evaluated in terms of their RNA/DNA ratio after one, five and twelve months, and used as an indicator of ecological function in the system. Crab condition in 0% sewage load was similar to that of wild crabs throughout, suggesting no significant effects of the mesocosms on their RNA/DNA ratio. Overall, both species coped well with the administered sewage loads, suggesting good ecological function in the system. Both species manifested similar patterns in RNA/DNA ratio, being more affected by seasonal fluctuations than by sewage load and vegetation presence and type. Higher RNA/DNA ratios were recorded in the long compared to the short rainy season. Sewage enhanced crab condition in the bare substratum and R. mucronata treatments, especially after one year, probably as a result of enhanced food availability. Uca inversa may be more sensitive to sewage pollution than U. annulipes. In A. marina, no difference in crab condition was observed between sewage loads, and this mangrove yielded the best reduction in sewage impacts. Our results support the usefulness of constructed mangrove areas in sewage treatment, especially if planted with A. marina and inhabited by physiologically healthy ocypodid crabs to enhance the system's performance.     

基于特定序列的长牡蛎视黄酸反应元件预测

视黄酸受体和核受体超家族中的大部分成员对细胞整个分化、增殖过程都具有调控功能。视黄酸受体结合配体后激活,通过结合靶基因启动子区特定的核苷酸序列调控靶基因表达。视黄酸受体结合序列是由核心序列[A/G]G[T/G]TCA间隔不同碱基构成的重复序列,称为视黄酸反应元件。为了实现对长牡蛎基因组中含有的视黄酸反应元件的快速筛选预测,本研究利用Perl工具编写了一个可以批量筛选视黄酸反应元件的脚本,并对长牡蛎基因组中启动子区域序列进行筛选预测,共筛选到412个启动子区含有视黄酸反应元件的基因。随后,将这些基因在各种数据库中比对分析,预测其参与的生物学过程及可能的生物学功能。结果显示,大部分基因与蛋白质结合、核苷酸结合、水解酶活性、蛋白激酶活性等功能有关。     

健脾柔肝息风汤对抽动障碍模型大鼠行为学及多巴胺受体mRNA表达的影响

目的：探讨健脾柔肝息风汤对抽动障碍模型大鼠运动性抽动的调节机制,为临床治疗抽动障碍提供实验依据。方法：将60只SD大鼠随机分为空白组,模型组,氟哌啶醇组,健脾柔肝息风汤低、中、高剂量组,每组各10只,除空白组外各组大鼠连续7 d腹腔注射亚氨基二丙腈（IDPN）造模,造模成功后连续灌胃6周,灌胃后第1、3、6周分别观察大鼠的行为学改变,采用RT-PCR法检测大鼠脑组织DRD1 mRNA、DRD2 mRNA含量。结果：灌胃6周后,与模型组比较,健脾柔肝息风汤中、高剂量组及氟哌啶醇组在运动行为评分、刻板行为评分及DRD1 mRNA含量方面均有所下降（P<0.05）;而健脾柔肝息风汤低、中、高剂量组及氟哌啶醇组在DRD2 mRNA含量方面有所下降（P<0.05）。结论：健脾柔肝息风汤可通过降低TD模型大鼠脑组织多巴胺DRD1 mRNA、DRD2 mRNA的含量,而改善TD模型大鼠运动性抽动行为。     

国内外岩溶塌陷监测方法综述

岩溶塌陷是全球广泛分布的地质灾害问题,严重影响和制约了岩溶地区社会、经济发展,因此,开展岩溶塌陷监测工作,对塌陷预测预报具有及其重要的作用。岩溶塌陷的监测技术与塌陷形成的机理关系密切。该文分析了岩溶塌陷的分布规律和形成的条件,系统总结了国内外岩溶塌陷的监测内容、技术方法、各自优缺点,如BOTDR监测、TDR监测、地质雷达监测、水动力条件监测及地-沉降等方法,为系统性地开展岩溶塌陷监测工作提供技术和方法参考,展望了未来的岩溶塌陷监测工作,认为岩溶塌陷监测需结合塌陷的形成机理、塌陷地区覆盖层特征及水动力条件,综合利用多种监测手段联合进行,并形成完善的监测网络。     

Effect of slow-solvent-vapour treatment on performance of polymer photovoltaic devices

In this work, enhanced poly(3-hexylthiophene):[6,6]-phenyl-C61-butyric acid methyl ester (P3HT:PCBM) bulk-heterojunction photovoltaic devices are achieved via slow-solvent-vapour treatment. The correlations between the morphology of the active layer and the photovoltaic performance of polymer-based solar cell are investigated. The active layers are characterized by atomic force microscopy and optical absorption. The results show that slow-solvent-vapour treatment can induce P3HT self-organization into an ordered structure, leading to the enhanced absorption and efficient charge transport.     

烟台市套子湾污水处理厂排海管道有关参数的确定

利用海洋处置城市污水是沿海地区一种把海洋水体的环境容量和自净能力用于环保的可行方法。烟台市套子湾污水处理厂1998年10月建成正式投入运转,日处理能力为25万m3,其污水排海管道工程是该厂重要组成部分,对污水排海管道的有关参数进行计算,可供沿海城市污水处理工程参考。     

海涂围垦工程中悬挂式爆破挤淤基础处理技术探讨

沿海软土地基围涂工程中悬挂式爆破挤淤基础处理断面结构设计中遇到的最大难题是对腰宽的处理,腰宽太窄则工后沉降大,反之,则断面石方量增大,投资增加,因此,试图通过工程实例说明悬挂式爆破挤淤基础处理断面设计及工程实施过程中遇到的问题及对策措施。