Expression of putative zinc-finger protein lcn61 gene in lymphocystis disease virus China （LCDV-cn） genome

An open reading frame (lcn61) of lymphocystis disease virus China (LCDV-cn), probably responsible for encoding putative zinc-finger proteins was amplified and inserted into pET24a (+) vector. Then it expressed in E. coli BL21 (DE3), and His-tag fusion protein of high yield was obtained. It was found that the fusion protein existed in E. coli mainly as inclusion bodies. The bioinformatics analysis indicates that LCN61 is C2H2 type zinc-finger protein containing four C2H2 zinc-finger motifs. This work provides a theory for functional research of lcn61 gene. Supported by High Technology Research and Development Program of China (863 Program, No. 2006AA100309)     

岩石变形破坏的熵突变过程与破坏判据

在岩石的变形破坏过程中,当进入不稳定的破裂发展阶段之后,系统不断调整结构抵抗外力的扰动,裂纹向局域集中的有序方向发展,应变能不断耗散,并以应变能的耗散为媒介,使系统与外扰动所追加的负熵流产生能量流通,也使系统熵值和系统的维数逐次降低,这一阶段岩石系统远离平衡态,岩石的破坏是系统熵的突变过程。基于这一认识,并在验证应变能分布与结构块度分布模式的一致性的基础上,推导出包含了结构因素和能量分布的熵表达式;对熵表达式进行平衡分析获得局部突变的分岔集,得到了岩石局部破坏的熵折迭突变破坏准则;同时,探讨了熵表达式所表征的结构有序度的尖点突变性,解出岩石系统的分岔集,这个分岔集就是岩石系统熵突变的整体破坏准则。     

Structure of the Potential Vorticity of an Explosive Cyclone over the Eastern Asian Region in Late November 2013

An explosive extratropical cyclone(EC)over the Eastern Asian region that caused two shipwrecks is analyzed using ERA-Interim reanalysis data from the European Center for Medium-Range Weather Forecasts.Analyses of the evolution of the EC reveal that the positive potential vorticity(PV)at the upper-tropospheric level displays a hook-shaped structure during the mature period of the cyclone.The PV distribution forms a vertically coherent PV structure called a PV tower.The vertical distribution of the PV can induce and strengthen cyclonic circulation from the lower-to upper-levels of troposphere,which is an important deepening mechanism of explosive cyclone.The PV tower occurs approximately ten hours prior to the development of surface occlusion in the cyclone.The evolution of surface fronts closely follows the development of the horizontal upper-tropospheric PV.This tandem development is largely attributed to the ability of the positive upper-tropospheric PV and the PV tower to induce cyclonic circulation simultaneously.The kinematic wrap-up process of cyclonic circulation also accelerates the formation of warm occlusion.A conceptual model of the distributions of positive PV and potential temperature combining the perspectives of dynamic tropopause folding,PV tower,and atmospheric stability,including westward tilting and baroclinicity,is proposed.This model can illustrate the explosive deepening mechanism of ECs.The regions of convective instability and rainfall determined by this model are consistent with those identified from the actual observation.     

An observational constraint on the existence of proto-superclusters at z =3.3

Observations have been conducted using the Ooty Radio Telescope in order to place constraints on the evolutionary scenario leading to the formation of the present day superclusters. The experiment attempted to detect 21 cm emission from massive neutral hydrogen condensates at a redshift ofz = 3.3. In an Einstein de-Sitter universe with baryon density Ω = 0.05, about ten condensates were expected in the volume surveyed if superclusters, having H I masses ≃5 × 10₁₅ M _⊙, were the first objects to separate out of the Hubble expansion. The sensitivity of our experiment rules out the existence of these condensates atz = 3.3 unless their lifetimes are less than one-tenth the dispersion in their epoch of formation or the proto-superclusters subtend angles greater than 6 arcmin. The result indicates that superclusters form at z > 3.3 if indeed they were the first objects to condense out of the Hubble flow. An erratum to this article is available at .     

鄂尔多斯盆地南部延长组富铀凝灰岩地球化学特征及形成机制

本文系统地研究了鄂尔多斯盆地南部延长组凝灰岩元素和同位素地球化学特征。结果表明：凝灰岩主量元素具有高K、高Al、Si中等至高、低Na的特征; REE总体特征为轻稀土富集、重稀土亏损,∑REE在4857～40212 μg/g 之间变化,Eu呈现出负异常,在034～082内变化,δCe范围在080～208之间; 微量元素中U含量在312～144 μg/g 之间,Ba、U、Th、Hf和Ce呈明显正异常,Nb、P、Ti和Rb呈现负异常。详细的分析表明：U的富集和一些微量元素如Ta、Dy、Lu等相关,Th与∑REE存在明显的正相关性,这些变化可能和盆地中铀矿的富集有关; 凝灰岩的硫同位素总体呈现深源特征。根据以上的研究对凝灰岩的形成原因及形成环境进行了探讨。鄂尔多斯盆地南部延长组凝灰岩来源于秦岭造山带火山作用,其中的铀元素富集是由于砂岩型铀矿中铀向凝灰岩迁移而被还原所致。本研究对盆地砂岩型铀矿的铀运移和富集机理认识具有一定指导意义。     

苏浙皖边界地区苏皖运动的有关问题

苏皖运动的存在于否,对研究苏浙皖边界地区的地质发展史和正确认识长兴阶地层有重要意义。笔者论证了长兴组的厚度变化、界线粘土层的性质与成因、底砾岩的确认和地层接触关系、古生物群的突变等问题,认为:1)区内苏皖运动是存在的;2)砺山-江阴海堤不可否定;将安徽广德牛头山经江苏宜兴至江阴条带内的龙潭组上部地层作为长兴组的相变产物是不妥的。     

The Reference Manure Concept for Transformation Tests of Veterinary Medicines and Biocides in Liquid Manure

Veterinary medicines and biocides are frequently applied in animal houses of livestock husbandry. Due to these application patterns, they reach liquid manure. So far these substances are not transformed during manure storage, they enter soil by the application of manure as organic fertilizer. Therefore, biodegradability of veterinary medicines and biocides in manure is in the focus of regulatory procedures of environmental risk assessment. Since the representative and reproducible sampling of manures from high volume tanks is considered impossible due to high matrix variabilities, the reference manure concept was developed to utilize liquid bovine and pig manures for reproducible laboratory testing. Accordingly, excrement samples of cattle and pigs individually kept at experimental and conventional animal houses were taken. Tap water was added to matrix characterized excrements in order to prepare bovine and pig reference manures of 10 and 5% dry substance contents, respectively. Subsequently, the long‐term transformation of selected ¹⁴C‐labeled test substances was investigated under strictly anaerobic conditions. The application of different batches of bovine or pig reference manures indicated that extrapolation of transformation rates within the same animal species was possible. However, results cannot be transferred from bovine to pig manure because of substance specific interactions with the different manure matrices. The dependency of metabolic dynamics on different dry substance contents (2.5, 5, 10%) was additionally investigated in both manure matrices. These tests clearly showed that the dry substance content of the reference manure is one of the most relevant factors affecting the transformation of veterinary medicines and biocides. In contrast to highly variable tank manures, the reference manure concept allows for the exact adjustment of this parameter to guarantee reproducible laboratory testing.     

Analysis of the positive ionospheric response to a moderate geomagnetic storm using a global numerical model

Current theories of F-layer storms are discussed using numerical simulations with the Upper Atmosphere Model, a global self-consistent, time dependent numerical model of the thermosphere-ionosphere-plasmasphere-magnetosphere system including electrodynamical coupling effects. A case study of a moderate geomagnetic storm at low solar activity during the northern winter solstice exemplifies the complex storm phenomena. The study focuses on positive ionospheric storm effects in relation to thermospheric disturbances in general and thermospheric composition changes in particular. It investigates the dynamical effects of both neutral meridional winds and electric fields caused by the disturbance dynamo effect. The penetration of short-time electric fields of magnetospheric origin during storm intensification phases is shown for the first time in this model study. Comparisons of the calculated thermospheric composition changes with satellite observations of AE-C and ESRO-4 during storm time show a good agreement. The empirical MSISE90 model, however, is less consistent with the simulations. It does not show the equatorward propagation of the disturbances and predicts that they have a gentler latitudinal gradient. Both theoretical and experimental data reveal that although the ratio of [O]/[N₂] at high latitudes decreases significantly during the magnetic storm compared with the quiet time level, at mid to low latitudes it does not increase (at fixed altitudes) above the quiet reference level. Meanwhile, the ionospheric storm is positive there. We conclude that the positive phase of the ionospheric storm is mainly due to uplifting of ionospheric F2-region plasma at mid latitudes and its equatorward movement at low latitudes along geomagnetic field lines caused by large-scale neutral wind circulation and the passage of travelling atmospheric disturbances (TADs). The calculated zonal electric field disturbances also help to create the positive ionospheric disturbances both at middle and low latitudes. Minor contributions arise from the general density enhancement of all constituents during geomagnetic storms, which favours ion production processes above ion losses at fixed height under day-light conditions.     

Invariant manifolds, phase correlations of chaotic orbits and the spiral structure of galaxies

In the presence of a strong   m = 2  component in a rotating galaxy, the phase-space structure near corotation is shaped to a large extent by the invariant manifolds of the short-period family of unstable periodic orbits terminating at L ₁ or L ₂. The main effect of these manifolds is to create robust phase correlations among a number of chaotic orbits large enough to support a spiral density wave outside corotation. The phenomenon is described theoretically by soliton-like solutions of a Sine–Gordon equation. Numerical examples are given in an N -body simulation of a barred spiral galaxy. In these examples, we demonstrate how the projection of unstable manifolds in configuration space reproduces essentially the entire observed bar–spiral pattern.