珠江口典型海岛周边水域浮游植物分布特征及其影响因素*

旅游业为海岛带来可观经济效益的同时, 人类活动也导致水体生态环境恶化, 如水体富营养化加剧、赤潮频发等。文章通过对珠江口东南部典型海岛——庙湾岛和外伶仃岛周边水域丰水期和枯水期现场环境数据与浮游植物分布特征的对比研究, 分析珠江径流等自然因素以及人类活动对河口天然海岛周边水体生态的潜在影响。枯水期外伶仃岛和庙湾岛周边水域海水分别镜检鉴定出76种和74种浮游植物, 两个海岛浮游植物平均细胞密度分别为2.62×10⁴个·L^-1和2.08×10⁴个·L^-1; 丰水期则分别鉴定出38种和47种浮游植物, 平均细胞密度分别为52.91×10⁴个·L^-1和170.57× 10⁴个·L^-1。在外伶仃岛和庙湾岛, 丰水期中肋骨条藻(Skeletonema coatatum)均为绝对优势种, 而枯水期两个岛的最主要优势种分别为窄隙角毛藻(Chaetoceros affinis)和新月筒柱藻(Cylindrotheca closterium), 物种多样性指数均明显高于丰水期。两个海岛微型浮游植物相对于其他两个粒级常占据优势地位, 但在丰水期, 小型浮游植物贡献明显上升, 其中外伶仃岛相对于枯水期由16.32%升至26.75%, 庙湾岛则由12.12%升高至24.78%。两个海岛在丰水期和枯水期均仅检出聚球藻(Synechococcus, Syn)和真核微微型藻类(eukaryotic, Euk)两大微微型浮游植物类群, 两者细胞密度分别为~10⁷个·L^-1及~10⁸个·L^-1量级。与环境因子的对比分析表明, 两个海岛浮游植物的区域分布与季节变化受多种因素影响, 其季节性差异主要受径流影响强度、影响范围以及相应的盐度、营养盐等环境因素的季节变化所调控。丰水期岛屿屏蔽效应对浮游植物丰度的区域分布特征有显著影响, 无论小型浮游植物还是微微型浮游植物均发现存在迎流面出现丰度高值分布的现象, 但对群落结构的分布影响不明显; 在枯水期, 水体环境很可能主要受人类活动与水体垂直混合扰动的综合影响, 总体上浮游植物分布的区域差异较小。     

2012-2016年调水调沙对黄河口海域大型底栖动物群落结构的影响

为了明确黄河调水调沙对河口海域大型底栖动物群落结构的影响,本研究于2012至2016年调水调沙后进行了大型底栖动物样品的采集工作。整个研究时期共鉴定出大型底栖动物7门138种,生物量和丰度2012年显著高于2016年,生物量年间无显著差异;研究时期内优势种以小型多毛类为主,鲜见软体动物、节肢动物和棘皮动物,其中2016年优势种全部为小个体多毛类;MDS显示,随时间的推移距河口不同距离梯度的大型底栖动物逐渐演变为相同的群落结构,且Shannon-Wiener多样性指数和Margalef丰富度指数呈下降趋势;CCA显示研究海域主要底栖动物种类的生态需求为高中值粒径、低溶解氧、pH、黏土和硫化物含量。本研究推测调水调沙工程一定程度上影响了底层生物群落结构,特别是研究海域的A断面。     

Stable Carbon Isotope and Long-Chain Alkane Compositions of the Major Plants and Sediment Organic Matter in the Yellow River Estuarine Wetlands

Elemental(TOC,TN,C/N)and stable carbon isotopic(δ^13C)compositions and long-chain alkane(n C16-38)concentrations were measured for eight major plants and a sediment core collected from the Yellow River estuarine wetlands.Our results indicate that both C3(-25.4‰to-29.6‰)and C4(-14.2‰to-15.0‰)plants are growing in the wetlands and C3 plants are the predominant species.The biomass of the wetland plants had similar organic carbon(35.5-45.8%)but very different organic nitrogen(0.35-4.15%)contents.Both C3 and C4 plants all contained long-chain alkanes with strong odd-to-even carbon numbered chain predominance.Phragmites australis,a dominant C3 plant contained mainly n C29 and n C31 homologues.Aeluropus littoralis,an abundant C4 plant were concentrated with n C27 and n C29 homologues.Organic matter preserved in the Yellow River estuarine sediments showed strong terrestrial signals(C/N=11-16,δ^13C=-22.0‰to-24.3‰).The distribution of long-chain n-alkanes in sediments also showed strong odd-to-even carbon chain predominance with n C29 and n C31 being the most abundant homologues.These results suggest that organic matter preserved in the Yellow River estuarine sediments were influenced by the wetland-derived organic matter,mainly C3 plants.The Yellow River estuarine wetland plants could play important role affecting both the carbon and nutrient cycling in the estuary and adjacent coastal waters.     

珠江口枯水期和丰水期中小型浮游动物群落动态

根据珠江口枯水期(2013年11月)和丰水期(2014年8月) 24个站位调查资料,对中小型浮游动物种类组成、丰度分布、群落结构及其主要影响因子进行了研究.结果表明,枯水期和丰水期分别鉴定浮游动物成体91种和70种,以桡足类最为丰富.浮游动物种类从河口上游向外围逐渐增加.浮游动物丰度时空分布差异显著,丰水期平均值高达11 619. 78 ind/m~3,显著高于枯水期的1 707. 13 ind/m~3.桡足类在珠江口中小型浮游动物中占绝对优势,枯水期和丰水期分别占总丰度的80. 3%和93. 0%,对浮游动物的丰度分布起关键作用.强额孔雀哲水蚤(Parvocalanus crassirostris)、中华异水蚤(Acartiella sinensis)和刺尾纺锤水蚤(Acartia spinicauda)在两个水期均为主要优势种,小拟哲水蚤(Paracalanus parvus)和厦门矮隆哲水蚤(Bestiolina amoyensis)则分别在枯水期和丰水期具有较高的优势度.聚类分析的结果显示,枯水期和丰水期该水域浮游动物均可划分为3个群落.相关性分析表明,珠江口中小型浮游动物群落结构受多个环境因子的影响,但盐度是其中最为关键的非生物因子.     

高通量测序分析北仑河口红树林潮间带底栖真核生物分布

为了解红树林不同潮位沉积物中底栖真核生物群落分布,基于18S rRNA基因采用高通量测序方法分析了广西北仑河口陆缘、林中和海缘3个潮位红树林沉积物中底栖生物群落结构。结果表明,北仑河口潮间带红树林沉积物中底栖生物多样性丰富,Shannon-Wiener指数变化范围在6. 08～6. 73之间; PCA分析表明潮间带中底栖生物群落差异较大,陆缘红树林中扁形动物、节肢动物和软体动物相对丰度较高,林中区域中纤毛虫、环节动物和轮虫相对丰度较高,海缘红树林中硅藻相对丰度较高;红树林中主要OTUs有桡足类的太平洋纺锤水蚤(Acartia pacifica)、硅藻类的海链藻(Thalassiosira sp.)、纤毛虫类的前管虫(Prorodon teres)、多毛类的小头虫(Capitella sp.)。高通量测序方法能较全面反映红树林区微型/小型底栖生物群落,研究结果为丰富红树林底栖生物群落研究和解析底栖生物在红树林生态系统发挥的作用提供基础数据。     

长江口盐度梯度下不同形态碳的分布、来源与混合行为

河口碳的生物地球化学过程是全球碳循环的重要组成。通过测定溶解无机碳（DIC）及其稳定同位素丰度（δ13C_DIC）,溶解有机碳（DOC）,有色溶解有机物（CDOM）,颗粒有机碳（POC）及其稳定同位素丰度（δ13C_POC）与元素比值（N/C）及相关指标,研究了2014年7月长江口盐度梯度下不同形态碳的分布、来源和混合行为。结果表明,DIC浓度、DOC浓度、POC含量分别为1 583.2~1 739.6 μmol/L,128.4~369.4 μmol/L和51.2~530.8 μmol/L,这些不同形态碳及CDOM的荧光组分的分布模式相似,均是从口内到口外,整体呈现先增大后减小的趋势,并与盐度呈现非保守混合行为。添加作用主要发生在在口门处最大浑浊带附近。与含量相反,从口内到口外,δ13C_DIC和δ13C_POC均呈现逐渐减小再增大的趋势,在口门附近达到最低值,分别为-9.7‰和-26.7‰。在口门附近不同形态碳含量上升及δ13C_DIC、δ13C_POC的降低可能主要与沉积物再悬浮及微生物作用有关。基于蒙特卡洛模拟的三端元混合模型的结果显示,河口内外POC来源变化明显,口内POC以陆源有机碳贡献为主,平均为62.3%,口外海源贡献逐渐增加。CDOM相关参数结果表明长江口CDOM主要来自陆源输入,海源及人类活动等也对其产生影响。     

九龙江口浮游植物群体对盐度胁迫的快速适应

Mixing of freshwater and seawater creates the well-known salinity gradients along the estuaries. In order to investigate how phytoplankton respond to the acute salinity changes, we exposed natural phytoplankton assemblies from the Jiulong River Estuary to differential saline field water while continuously monitoring their photosynthetic performances under both indoor-and outdoor-growth conditions. When the natural cell assemblies from salinity 30 field water were exposed to series low saline field water(salinity 25, 17, 13 and 7.5), the effective Photosystem II quantum yield(ΔF/F_m′) decreased sharply, e.g., to one-fifth of its initials after 5 min exposure to salinity 7.5 field water, and then increased fast during the following 40 min and almost completely recovered after 320 min. During such an exposure process, non-photochemical quenching(NPQ) sharply increased from 0 to 0.85 within 5 min, and then decreased to nearly 0 within the following 70 min. When these cells re-acclimated to salinity 7.5 field water were exposed to series high saline field water(salinity 13, 17, 25 and 30), a similar response pattern was observed, with the decreased ΔF/F_m′ accompanied with increased NPQ, and followed by the recovery-induced increase in ΔF/F_m′ and decrease in NPQ. A similar response pattern as ΔF/F_m′to the acute osmotic stress was also observed in the photosynthetic carbon fixation capacity according to radiocarbon(~(14)C) incorporation. Our results indicate that estuarine phytoplankton assemblies could rapidly recover from the acute osmotic stress, implying a potential cause for their frequent blooms in coastal-estuarine waters where despite drastically varying salinity, available nutrients are abundant due to the land-derived runoffs or mixing-caused relaxations from sediments.     

我国瓯江口和珠江口凤鲚洄游模式和生境利用研究

Habitat use of the tapertail anchovy(Coilia mystus Linnaeus, 1758) from the Oujiang River Estuary and the Zhujiang(Pearl) River Estuary was studied by examining the environmental signatures of Sr and Ca in otoliths using electron probe microanalysis. Individuals from the Oujiang River had higher and varied Sr:Ca ratios(expressed as(Sr:Ca)×1 000, 3.83–13.0 average) in the otolith core regions, suggesting that they were born in brackish or sea waters, and that a freshwater habitat might not be necessary for egg hatching and larval growth.While, individuals from the Zhujiang River had lower Sr:Ca ratios(0.39–2.51 average) in the core regions,suggesting a freshwater origin. After hatching, anchovies from the Zhujiang River migrate downstream to the river estuary close to brackish water. Our results demonstrated varied habitat use for spawning during stages of early life history between the two populations, and suggested that such variations are promoting diversity of life history strategies of this species.     

近50 a来辽河口水下三角洲演变特征研究

本文基于近50 a来的辽河口海域的水下地形资料,采用GIS技术手段研究了辽河口水下三角洲的演变特征。研究结果表明:近50 a来辽河水下三角洲外缘(蛤蜊岗子滩西缘和南缘、西滩西缘和南缘)出现较大幅度的侵蚀后退;蛤蜊岗子滩东缘淤积外移,南部滩尾东部有北移东偏趋势;近岸浅滩由淤涨变为基本稳定,局部有所侵蚀后退;受蛤蜊岗子滩向东淤进的影响,蛤蜊岗子滩东侧深槽有所缩窄;蛤蜊岗子滩和西滩之间深槽水域出现拓宽变浅的变化趋势。流域来水来沙减少是近年来辽河口水下三角洲外缘侵蚀后退的主要原因,外缘侵蚀泥沙在波流作用下向河口搬运是蛤蜊岗子滩东侧淤积、深槽缩窄的主要原因,近岸边滩人工围垦对水下三角洲演变的影响值得关注。     

长江口横沙东滩典型海堤越浪量现场和试验研究

越浪量是允许越浪海堤设计的重要参数。在对以往相关研究成果进行回顾和分析基础上,结合长江口横沙东滩促淤圈围五期工程现场波浪和越浪量实测资料,通过对横沙东滩典型断面型式进行整体和断面物理模型试验,研究结构各参数对越浪量的影响,进而提出适合长江口海堤的越浪量计算公式。公式计算结果与试验数据吻合良好,与Van der Meer公式相比具有较好的一致性。